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Ecosystem services (ES) are a fundamental component of well-being and sustainable urban development with tremendous potential to enhance urban planning. Recently, several studies have evaluated the environmental performance of urban plans using the ES approach. To strengthen this science-policy integration, it is still necessary to perform ES assessments within the urban planning practice as well as to collect empirical evidence on the impacts of envisioned planning measures on the supply of ES in urban environments across the world. In this research, we analyzed the state-of-the-art of China’s new environmental governance, which aims to change China’s land use policy and particularly the role of Green Infrastructure (GI) regarding urban planning and ES. We focused on the Shanghai Baoshan district Master Plan as a case study, and analyzed it under the lenses of the supply of ES using the matrix approach. We ascertained the supply of ES as delineated in the ecological network plan for 2035, and developed an evaluation framework based on CICES v5.1 and two expert workshops. Our approach used an integrated preliminary ES-assessment, and evaluated the consequences for the supply of ES in Baoshan district, which is adaptable to varying urban geographies. The results of our assessment show that, if realized as planned, the district will increase the overall supply of ES, especially regulating and cultural services, that play an important role within GI on the urban level. In general, the land use plans should include fine-grained information within building blocks to allow for even better assessing of the spatial structure of the supply of ES

Pyruvate kinase (PK) is one of the three rate-limiting enzymes of glycolysis, and it plays a pivotal role in energy metabolism. In this study, we have identified 10 PK genes from the rice genome. Initially, these genes were divided into two categories: cytoplasmic pyruvate kinase (PKc) and plastid pyruvate kinase (PKp). Then, an expression analysis revealed that OsPK1, OsPK3, OsPK4, OsPK6, and OsPK9 were highly expressed in grains. Moreover, PKs can form heteropolymers. In addition, it was found that ABA significantly regulates the expression of PK genes (OsPK1, OsPK4, OsPK9, and OsPK10) in rice. Intriguingly, all the genes were found to be substantially involved in the regulation of rice grain quality and yield. For example, the disruption of OsPK3, OsPK5, OsPK7, OsPK8, and OsPK10 and OsPK4, OsPK5, OsPK6, and OsPK10 decreased the 1000-grain weight and the seed setting rate, respectively. Further, the disruption of OsPK4, OsPK6, OsPK8, and OsPK10 through the CRISPR/Cas9 system showed an increase in the content of total starch and a decrease in protein content compared to the WT. Similarly, manipulations of the OsPK4, OsPK8, and OsPK10 genes increased the amylose content. Meanwhile, the grains of all CRISPR mutants and RNAi lines, except ospk6, showed a significant increase in the chalkiness rate compared to the wild type. Overall, this study characterizes the functions of all the genes of the PK gene family and shows their untapped potential to improve rice yield and quality traits.

High levels of ammonium (NH

Industrial transfer is an important driving force for competitiveness promotion of the manufacturing at Zhejiang. Based on the theoretical model established, effects of industrial transfer on different dimensions of competitiveness were empirically analyzed from aspects of the direction and the hierarchy. The results showed that it was positively related to the industrial capacity competitiveness and the earning competitiveness, negatively related to the external competitiveness and not related to the internal competitiveness, with effect on the intensive being significantly stronger than that on the extensive. At the same time, there was breakage in transmission path of industry transfer on promotion of competitiveness of the manufacturing.

This research exhibits the design of a feasible, enzyme-free and sensitive fluorescent sensing assay for the detection of Staphylococcus aureus (S. aureus), using self-circulation of molecular beacons. With protein A on S. aureus as identifying target, the capture probe binds on the surface of S. aureus based on interaction between its aptamer section and protein A. Recognition of protein A by aptamer section in capture probe leads to allosterism of capture probe, exposing initiator section to activate the following self-circulation. After multiple circulation-based signal amplification, the method exhibits a favorable detection sensitivity and shows a promising prospect for the keratitis-related pathogenic bacteria detection. The highlights of the sensing assay are as follows: (i) capture probe is designed with aptamer section which endows the method a high selectivity; (ii) signal of bacteria is converted to nucleic acid signal after recognition of target bacteria by capture probe; and (iii) high sensitivity of method is derived from the self-circulation process. Therefore, we believe that the strategy can provide a useful platform for target bacteria detection and thus contribute to the diagnosis of infectious diseases.

Recreational green spaces are associated with human thriving and well-being. During the ongoing COVID-19 pandemic a spotlight has been shed on the interconnection between access to these spaces, human well-being and social equity. Containment measures enacted in many cities effectively precluded people from reaching distant recreational areas during the pandemic and consequently, recreational areas close to home became particularly important. Urban density is often associated with building or population density with the assumption that if either parameter has a high value, the availability of open (green) space is low. Certain densities are associated with specific spatial qualities. Addressing challenges of sustainable development, a detailed evaluation of density is necessary to allow evidence-based arguments, planning and decision-making. In this study we develop a multi-scale analysis method for quantifying and assessing green infrastructures from settlement unit to building level to reach a differentiated view on density, arguing that density can be organized in different ways achieving very different qualities. For this purpose, we use geospatial-data analysis and in-depth neighborhood studies to compare two cities in Asia and Europe, revealing different ways of organizing density in the built environment and identifying a derivation of approaches for sustainable development in dense urban regions.

Starch and storage proteins are the main components of rice (Oryza sativa L.) grains. Despite their importance, the molecular regulatory mechanisms of storage protein and starch biosynthesis remain largely elusive. Here, we identified a rice opaque endosperm mutant, opaque3 (o3), that overaccumulates 57-kDa proglutelins and has significantly lower protein and starch contents than the wild type. The o3 mutant also has abnormal protein body structures and compound starch grains in its endosperm cells. OPAQUE3 (O3) encodes a transmembrane basic leucine zipper (bZIP) transcription factor (OsbZIP60) and is localized in the endoplasmic reticulum (ER) and the nucleus, but it is localized mostly in the nucleus under ER stress. We demonstrated that O3 could activate the expression of several starch synthesis-related genes (GBSSI, AGPL2, SBEI, and ISA2) and storage protein synthesis-related genes (OsGluA2, Prol14, and Glb1). O3 also plays an important role in protein processing and export in the ER by directly binding to the promoters and activating the expression of OsBIP1 and PDIL1-1, two major chaperones that assist with folding of immature secretory proteins in the ER of rice endosperm cells. High-temperature conditions aggravate ER stress and result in more abnormal grain development in o3 mutants. We also revealed that OsbZIP50 can assist O3 in response to ER stress, especially under high-temperature conditions. We thus demonstrate that O3 plays a central role in rice grain development by participating simultaneously in the regulation of storage protein and starch biosynthesis and the maintenance of ER homeostasis in endosperm cells.

BACKGROUND In recent years, the incidence of gastrointestinal (GI) cancer in China has increased annually. Early detection and appropriate therapy are considered to be the key to treat GI cancer. DNMT1 takes an active part in the advancement of GI cancer, which will change as the disease progresses. But its expression characteristics in the dynamic variations of GI carcinogenesis are still unclear. AIM To investigate the expression characteristics of DNMT1 in different GI diseases. METHODS We detected the expression of DNMT1 in 650 cases of different GI diseases by immunohistochemistry, including 90 cases of chronic superficial gastritis (CSG), 72 cases of atrophic gastritis with intestinal metaplasia (AG/GIM), 54 cases of low-grade intraepithelial neoplasia (GLIN), 66 cases of high-grade intraepithelial neoplasia (GHIN), 71 cases of early gastric cancer (EGC), 90 cases of normal intestinal mucosa (NIM), 54 cases of intestinal low-grade intraepithelial neoplasia (ILIN), 71 cases of intestinal high-grade intraepithelial neoplasia (IHIN), and 82 cases of early colorectal cancer (ECRC). RESULTS In the CSG group, all cases showed weakly positive or negative expression of DNMT1. However, in other four groups (AG/GIM, GLIN, GHIN, and EGC), the positive expression rate gradually increased with the severity of the diseases; the negative or weakly positive cases accounted for 55.56% (40/72), 38.89% (21/54), 1.52% (1/66), and 1.41% (1/71), respectively. Besides, the moderately positive cases were 44.44% (32/72), 57.41% (31/54), 80.30% (53/66), and 43.66% (31/71), respectively. The strongly positive cases only existed in the GLIN (3.70%, 2/54), GHIN (18.18%, 12/66), and EGC (54.93%, 39/71) groups. The differences between any two groups were statistically significant (P < 0.05). Similarly, in the NIM group, cases with weakly positive expression of DNMT1 were predominant (91.11%, 82/90), and the rest were moderately positive cases (8.89%, 8/90). In the ILIN, IHIN, and ECRC groups, the rates of cases with weak or negative expression of DNMT1 were 46.30% (25/54), 12.68% (9/71), and 4.88% (4/82), respectively; with moderately positive expression were 53.70% (29/54), 71.83% (51/71), and 34.15% (28/82), respectively; and with strongly positive expression were 0.00% (0/54), 15.49% (11/71), and 60.98% (50/82), respectively. The differences between any two groups were also statistically significant (P < 0.05). CONCLUSION The overexpression of DNMT1 protein could effectively predict early GI cancers and severe precancerous lesions, which may have potential clinical application value.

Purpose: The study was conducted to investigate the relationship of serum pepsinogens PGI, PGII, gastrin-17, and Hp-IgG with colorectal cancer (CRC), aiming to explore the clinical significance of serum markers reflecting gastric function and H. pylori infection in CRC. Methods: A total of 569 CRC cases and 569 age and sex-matched controls were enrolled in this study between June 2012 and April 2016 from The First Hospital of China Medical University. The serum markers reflecting gastric function and H. pylori infection were detected using ELISA, including PGI, PGII, PGI/II ratio, G-17 and Hp-IgG. Information of clinicopathological parameters and tumor biomarkers was collected from the medical records of inpatients, including CEA, CA199, CA125, CA153 and AFP. Results: Serum PGII, G-17 levels and Hp-IgG were increased in CRC, while PGI and PGI/II ratio appeared no significant difference between CRC and controls. In subgroup analysis, PGII was more significant in males (P=0.014). Hp-IgG was demonstrated higher in age

Population ageing and shrinking are demographic phenomena with far-reaching implications for sustainability in the current context of extensive and rapid urbanization. This Perspective rationalizes their interface by (a) identifying the challenges and opportunities that ageing and shrinking urban populations will have for implementing the sustainable development goals (SDGs), and (b) discussing some emerging interventions to capitalise on the opportunities and reduce the challenges to achieving sustainability. We argue that a diverse set of context-specific technological, socioeconomic, institutional and governance interventions would be needed to leverage effectively the opportunities and minimize the risks posed by ageing and shrinking urban populations for long-term sustainability.

Sulforaphane (SFN) extracted from broccoli sprout has previously been investigated for its potential properties in cancers, however, the underlying mechanisms of the anticancer activity of SFN remain not fully understood. In the present study, we investigate the effects of SFN on cell proliferation, cell cycle, cell apoptosis, and also the expression of several cell cycle and apoptosis-related genes by MTT assay, flow cytometry and western blot analysis in gastric cancer (GC) cells. The results showed that SFN could impair the colony-forming ability in BGC-823 and MGC-803 cell lines compared with the control. In addition, SFN significantly suppressed cell proliferation by arresting the cell cycle at the S phase and enhancing cell apoptosis in GC cells in a dose-dependent manner. Western blot results showed that SFN treatment significantly increased the expression levels of p53, p21 and decreased CDK2 expression, which directly regulated the S phase transition. The Bax and cleaved-caspase-3 genes involved in apoptosis executive functions were significantly increased in a dose-dependent manner in BGC-823 and MGC-803 cells. These results suggested that SFN-induced S phase cell cycle arrest and apoptosis through p53-dependent manner in GC cells, which suggested that SFN has a potential therapeutic application in the treatment and prevention of GC.

Objective The aim of this study was to investigate the value of the combined expression of the gastric mucosal differentiation protein pepsinogen C (PGC) and gastric cancer (GC)-associated antigen MG7 for the diagnosis of GC and prediction of the development from precancerous conditions to GC. Methods The gastric mucosal biopsies of 285 subjects enrolled from a region with a high incidence of GC were obtained and histopathologically examined. Subjects testing negative for GC (n=208) were followed up from 1998 to 2015. The levels of PGC and MG7 in the biopsies were determined by immunohistochemistry. Results PGC was positive in 91.4% of the non-atrophic gastritis, 26.5% of the atrophic gastritis, and 0% of the GC. MG7 was positive in 15.0% of the non-atrophic gastritis, 82.4% of the atrophic gastritis, and 94.8% of the GC. The non-atrophic gastritis group was predominantly "PGC+MG7-". The atrophic gastritis and GC groups were predominantly "PGC-MG7+". The rate of GC in subjects with "PGC-MG7+" staining was 113.4-fold higher [95% confidence interval (95% CI): 15.3-869.4, P

This study examines the financial rights and responsibilities of China’s local villages and townships based on a field survey conducted in Xingguo County. This field study focussed on local revenues and expenditures from 2012 to 2014. Upon analyzing data from the survey, we noted numerous difficulties of local financial management due to a lack of well-configured financial authority and power among central and local governments. In addition, revenues and expenditures are unbalanced at the local government level in China. Rural financial management systems need to be improved, and the rural finance function should be strengthened.

Multidimensional interactions of multiple factors are more important in promoting cancer initiation. Gene‐gene interactions between protein‐coding genes have been paid great attention, while rare studies refer to the interactions between encoding and noncoding genes. Our research group previously found encoding gene PGC polymorphisms could affect the susceptibility to atrophic gastritis (AG) and gastric cancer (GC). Interestingly, several SNPs in long noncoding RNA (lncRNA) genes, just adjacent to PGC, were found to be associated with AG risk and GC prognosis afterward. This study aims to explore the SNP interactions between PGC and its neighbor lncRNAs on the risk of AG and GC. Genotyping for seven PGC SNPs and seven lncRNA SNPs was conducted using Sequenom MassARRAY platform in a total of 2228 northern Chinese subjects, including 536 GC cases, 810 AG cases, and 882 controls. We found 15 pairwise PGC‐lncRNAs SNPs had interactions: Five pairs were associated with AG risk, and ten pairs were associated with GC risk. Moreover, two GC‐related interactions PGC rs6939861 with lnc‐C6orf‐132‐1 rs7749023 and rs7747696 survived the Bonferroni correction (P correction = 0.049 and 0.007, respectively). Several combinations showed obvious epistasis and cumulative effects on disease risk. Some three‐way interactions of SNPs with smoking and drinking could also be observed. Besides, a few interacting SNPs showed correlations with the expression levels of PGC protein and related lncRNAs in serum. Our study would provide research clues for further screening combination biomarkers uniting both protein‐coding and noncoding genes with the potential in prediction of the susceptibility to GC and its precursor.

A compact optical fiber sensor based on a Fabry–Perot interferometer (FPI) with an integrated fiber Bragg grating is proposed to measure pressure and temperature simultaneously. The light reflectors of the proposed FPI comprise a graphene film and a fiber end face. The sensitivities of the FPI sensor for measuring pressure and temperature are 501.4 nm/kPa and 306.2 nm/°C, respectively. Conflicting signals of pressure and temperature can cause cross-impact errors at 610 Pa/°C. The introduction of a fiber Bragg grating can effectively prevent the cross-impact of the FPI sensor. The pressure sensitivity obtained is 7.96 to 12 times higher than the previous study. The proposed fiber sensor can simultaneously measure pressure and temperature using a sensitivity matrix.

This paper aims to make clear the effects of two important parameters, namely thickness of soil layer and leaf area index of plant layer, on green roof energy and thermal performance. Based on a coupled hygrothermal transfer model validated by field experiments in Shanghai area, energy balance of plant and soil layer is analyzed. Then 18 cases are simulated for different combinations of soil thickness and leaf area index, the results show that soil thickness has a significant effect on long term thermal performance of green roof in both summer and winter, while the effect of leaf area index is only great in summer. Compared with the hypothetical case without evaporation and transpiration, it is found that evaporation of soil layer makes a greater contribution in summer but a less one in winter compared with insulation effect of soil layer. For plant layer, shading is the main cooling way of roof deck while transpiration plays a minor role. Finally, based on the simulation results of long-term thermal performance, two new indexes are proposed to evaluate the relative thermal characteristic of green roof. And the influences of soil thickness, leaf area index and evapotranspiration on the two indexes are analyzed respectively.

It's widely accepted that green roof systems exhibit varying thermal performances compared with common roofs, nonetheless, there are lack of indexes to characterize this difference with regards to internal mechanisms. In light of this condition, this paper presents two new indexes, namely insulation factor ( φ ) and comprehensive temperature regulation factor ( β ) based on insulation and passive cooling effect of the green roof. In order to predict long-term thermal performance of green roof in Shanghai area, a coupled heat and moisture transfer model is developed and validated by measurement data. Based on this model, green roof thermal performances are simulated under different indoor temperatures, and two indexes under different seasons are calculated. The results demonstrate that φ changes little around the year while β varies greatly. To make clear the variation rule of these two indexes, a sensitivity test is carried out. It's observed that thermal resistance of substrate layer and corresponding common roof are the main influence parameters of φ , while β is affected mostly by leaf area index, surface reflectivity and emissivity of substrate layer and common roof. In practice, these two indexes could be used to evaluate the relative thermal performance of different types of green roof against corresponding common roof.

As a building component with long history, green envelope has received much attention for its thermal and energy benefits in the past decades. However, few studies have taken into account the two-way effect of green envelope on indoor and outdoor environment during thermal performance evaluation. In this paper, a coupled hygrothermal transfer model of green envelope is developed and validated against field measurements, and the results demonstrate that the model could reasonably reproduce temporal variations of temperature and moisture in green envelope. Then the green envelope model is implemented into an urban canopy model to predict its thermal performance under urban canopy context in Shanghai area. The simulations for a district of office buildings indicate that green envelope decreases sensible heat getting into the indoor and outdoor space significantly, and the maximum indoor temperature difference between buildings with and without green envelope is up to 0.34 °C under free-floating condition. Meanwhile, for outdoor environment, the maximum differences for air temperature, mean radiant temperature and SET* are up to 0.1,0.9 and 0.2 °C respectively under air conditioning condition. What’s more, sensitivity analysis shows that building density has a significant effect on the relative cooling benefit of green envelope against common envelope.