Search

Objective: To investigate the expression of long non-coding RNA LOC101927476 (LncRNA LOC101927476) in ovarian cancer and its effect on the biological characteristics of ovarian cancer. Methods: Patients with ovarian cancer who underwent surgery in Cancer Hospital of Chinese Academy of Medical Sciences from 2018 to 2019 were selected. The expressions of LOC101927476 in ovarian cancer cells 3AO, OVCA429, TOV21G, A2780, SKOV3, as well as 22 primary tumor tissues and their matched metastatic tumor tissues were detected by real-time quantitative polymerase chain reaction (RT-PCR). Ovarian cancer transcriptome sequencing data from the TCGA database was used to verify the expressions of LOC101927476 and GATA4. 3AO and OVCA429 cells were infected with lentivirus plasmid containing OE-LOC101927476 and single guide RNA (sg-RNA) targeting LOC101927476, respectively. The effects of LOC101927476 on migration and invasion were detected by Transwell and wound healing assay. The effect of LOC101927476 on cell proliferation was detected by cell counting kit-8 (CCK-8) assay. Results: RT-PCR assay showed that 20 out of 22 patients had significantly lower expression of LOC101927476 in their metastatic tumors compared with primary tumors. Transwell assay showed that overexpression of LOC101927476 significantly inhibited the invasion and migration capacities of 3AO cells. The numbers of invading and migrating 3AO cells infected with OE-LOC101927476 lentivirus were (357±63) and (699±65), respectively, lower than (661±95) and (1 024±76) in OE-EV group ( P <0.050). In contrast, the numbers of invading and migrating OVCA429 cells with LOC101927476 knockdown were (512±72) and (472±40), respectively, higher than (309±13) and (363±27) in sg-Control group ( P <0.050). Wound healing assay results showed that after 48 hours, the percentage of scratch healing of 3AO cells in OE-LOC101927476 group was (10.86±0.63)%, significantly lower than (57.38±4.42)% of OE-EV group ( P =0.009). After 24 hours, the percentage of scratch healing of OCVA429 cells in sg-LOC101927476 group was (59.98±1.34)%, significantly higher than (23.15±2.03)% of sg-Control group ( P =0.004). CCK-8 assays showed that the OD value of 3AO cells in OE-LOC101927476 group was (2.07±0.08), significantly lower than (2.29±0.04) of OE-EV group ( P =0.009). The OD value of OVCA429 cells in sg-LOC101927476 group was (2.13±0.03), significantly higher than (1.93±0.03) of sg-Control group ( P =0.001). The relative expression of GATA4 in OE-LOC101927476 group was (1.86±0.25), significantly higher than 1.00 of OE-EV group ( P =0.001). In patients with high expression of LncRNA LOC101927476, the expression level of GATA4 was (2.93±0.35), which was higher than (0.29±0.06) of LOC101927476 low expression group ( P =0.001). Conclusion: LncRNA LOC101927476 can inhibit the invasion, migration and proliferation of ovarian cancer cells.

The effect of hydraulic retention time (HRT) on denitrifying phosphorus and nitrogen removal in a modified two sludge A 2 /O-BAF system was studied. The influent COD, NH 4 + -N, and TP were 189.6, 60.4, and 5.1 mg·L -1 , respectively. When HRT was 9, 8, 7, and 6 h, the average effluent COD was less than 42 mg·L -1 . The average effluent NH 4 + -N levels were 2.4, 2.8, 3.3, and 6.5 mg·L -1 , respectively. The average effluent TP values were 0.3, 0.4, 0.7, and 0.8 mg·L -1 , respectively. The ratio of the denitrifying phosphorus accumulation organisms (DPAOs/PAOs) in the system of anoxic zone was reduced from 76.8% to 48.8%. When HRT was 8 h, the ratio of denitrifying phosphorus to nitrogen (ΔPO 4 3- /ΔNO 3 - -N) was increased by 37.5% by a mathematical statistics method. The ΔPO 4 3- /ΔNO 3 - -N in the anoxic zone was 1.24 (the theoretical value is 1.41). At this time, the effect of denitrifying phosphorus to nitrogen was the best. The SVI value was lower than 100 mL·g -1 throughout the experiment, and the MLVSS/MLSS gradually decreased from 0.74 to 0.63, indicating that the sludge activity was reduced.

A modification of the two sludge A 2 /O-BAF system was used to treat low C/N real domestic sewage. In order to improve the utilization of the carbon source, the effects of two step feeds (pre-anoxic zone and anoxic zone) on denitrifying phosphorus and nitrate removal were studied. According to the formula of material balance for COD, the utilization of carbon source was analyzed and evaluated under different ratios of step feed, simultaneously. The results showed that when the ratio of step feed was 7:3 and the influent concentrations of COD, NH 4 + -N, TN, and TP were 174.99, 58.19, 59.10, and 5.15 mg·L -1 , respectively, their effluent concentrations were 29.48, 4.07, 14.10, and 0.40 mg·L -1 , and the removal rates were 82.12%, 92.76%, 75.45%, and 91.20%, respectively. It was found that when the ratio of the denitrifying phosphorus accumulation organisms to the phosphorus accumulation organisms(DPAOs/PAOs) was 98.81%, the efficiencies of denitrifying phosphorus and nitrate removal were optimum. By optimizing step feed, the carbon source was utilized effectively, and the efficiencies of nitrogen and phosphorus removal were improved simultaneously. The theoretical basis has thus been provided for the modification of the two sludge A 2 /O-BAF system to treat low C/N waste water.

Shortcut nitrification sludge, which was set aside for two months, was recovered using Reactors Ⅰ, Ⅱ, Ⅲ, and Ⅳ. The aeration rates of Reactors Ⅰ, Ⅱ, Ⅲ, and Ⅳ were 120, 100, 80, and 60 L·h -1 , respectively, while treating real domestic sewage, and the ratio of aerobic/anoxic was 30 min/30 min at the temperature of 25℃. The influent of ammonia was 50-80 mg·L -1 , and the concentration of effluent ammonia was stable, at below 5 mg·L -1 , after the 12 th , 18 th , 21 st , and 21 st cycles. The removal ratio of ammonia nitrogen was about 95%. The highest concentrations of nitrite for Reactors Ⅰ, Ⅱ, Ⅲ, and Ⅳ were 20.83, 22.81, 21.50, and 20.73 mg·L -1 , respectively, which occurred in the 30 th , 35 th , 38 th , and 42 nd cycles, respectively. The concentrations of effluent nitrate were lower than 0.5 mg·L -1 , and the nitrite accumulation rates were higher than 99%. The activity of ammonia-oxidizing bacteria (AOB) increased gradually and finally stabilized at 100.00%; however, the activity of nitrite-oxidizing bacteria (NOB) was gradually inhibited. The recovery of shortcut nitrification was achieved successfully in the different aeration modes.

Real domestic sewage was treated with sequencing batch reactors (SBR). The aerobic/anoxic modes were alternated 4 times at 30℃ and 7 times at 18℃ in a single-cycle, respectively, and the ratio of aerobic/anoxic cycles was 30 min/30 min. The influent concentration of ammonia and nitrite was 61.44 mg·L -1 and 0.77 mg·L -1 , respectively. After the experiments were run for 61 and 90 cycles, the effluent of the ammonia was 0.68 mg·L -1 and 1.28 mg·L -1 and the removal rate for ammonia was 98.94% and 99.57%, respectively. The nitrite concentrations were 20.57 mg·L -1 and 20.18 mg·L -1 , and the nitrite accumulation rate reached 95.92% and 99.58%, respectively. During shortcut nitrification, the activity of the ammonia oxidizing bacteria (AOB) increased gradually before finally stabilizing at 100.00%, however the activity of nitrite oxidizing bacteria (NOB) increased first and then decreased gradually, the activity of AOB exceeded NOB at 32 and 74 cycles respectively, where the AOB became the dominant bacteria and the activity of NOB was completely inhibited at 61 and 90 cycles.

In the imaging process of satellite-based pushbroom hyperspectral imager, attitude motions of satellite platform, represented by vibrations, will cause aliasing of the object information comes from different sub-areas of the detector, leading to degradation of hyperspectral image quality. In order to suppress and correct the imaging errors caused by satellite vibrations more effectively, spatial and spectral degradation mechanisms of typical dispersive pushbroom imaging spectrometer caused by satellite vibration are studied in this paper, including theoretical simulation and experimental study. With the analysis of spectral mixing process during exposure, the relationship between spectrum of ground object and satellite attitude is obtained, and a degradation model of pushbroom spectral imaging is presented. The effects of different attitudes of vibration are considered in the degradation model. Mean mixing ratios of each pixel are easy to calculate with a universal coefficient matrix, as long as the satellite attitude parameters of each moment are known. Then the simulation degraded spectral image data cube is achieved. The common expression of mean mixing ration is derived in detail. More important, the effects of vibration amplitude and frequency are quantitative analyzed separately. Degraded simulation and ground simulation experiments are carried out based on real hyperspectral data cubes, then the quality of the cubes before and after degradation are evaluated. Results show that simulation is in good agreement with reality. Mean mixing ratio can reflect the degradation extent of hyperspectral data directly. The satellite vibrations bring about spatial quality deteriorate of hyperspectral image, and lead to the aliasing of spectrum comes from different ground object. The degradation extent of hyperspectral data is determined mainly by vibration amplitude. The influence of frequency is limited.

Thermospheric O/N2 can be retrieved from the radiance of OI 130.4 nm dayglow. In the paper, the algebraic relationship between the two variables is established, and the characteristics of the radiance varying with observing angle and solar zenith angles are discussed, based on which the look-up tables of the regularization factors are made up, thus putting up a method for retrieving thermospheric O/N2 from measurements of OI 130.4 nm dayglow and meanwhile introducing a correcting coefficient in the algorithm to dismiss the error brought about by the difference between the ideal atmosphere representative by NRLMSISE-00 and the real atmosphere. Finally, the simulated dayglow radiance data of Oct 1, 2002 are used as an example to testify the validation of the algorithm. The results demonstrated that the algorithm is able to reconstruct most of the features of O/N2 and has an obvious advantage over the conventional algorithm.

The present paper proposed a spectral degradation theory based on the point spread matrix, which is developed from the simulation algorithm of degradation based on differential dynamic spectral imaging for moving imaging. According to the eight neighborhood mixing model, considering the varying movement with time of the satellite platform, the situation that different object pixel has different point spread matrix is corresponding to the concept of the pseudo cross-correlation theory. Thus, the pseudo cross-correlation degradation theory of moving spectral imaging is presented. In this method, the point spread matrix is constructed by the mean mixing ratio of eight neighborhoods, which can be calculated by the POS data on satellite using the differential imaging theory based on image motion matrix. When calculating the point spread matrix with the simulating POS data curve, we found out that the point spread matrix can be simplified according to the importance of mixing effect to reduce calculation. Then, the presentation and computing method of the pseudo cross-correlation degradation theory of moving spectral imaging is clearly articulated, and the result of degradation simulating and calculating is evaluated qualitatively and quantitatively from imaging dimension and spectral dimension respectively, and the similarity between the degradation image and the initial image is revealed using the SSIM parameter. The pseudo cross-correlation degradation theory of moving spectral imaging improves the existing problem of spectral imaging degradation based on the complex movement of satellite platform. The simulation results show that this kind of degradation theory is suitable for solving the problem of spectral imaging degradation based on the complex satellite platform movement completely.

Magnetic storms usually cause significant departures of thermospheric O and N2 from their normal values. To study the effects on thermospheric neutral species caused by magnetic storms, a method to retrieve thermospheric O/N2 based on the data obtained from global ultraviolet imager on board TIMED is presented. With the help of AURIC, the normalizations of observing angles and SZAs were preformed to the measurements and a relationship between 135.6/LBHs and O/N2 was established. Finally, applying the proposed method to retrieve O/N2 during a magnetic period (29, September - 4, October, 2002), it was shown that magnetic storms could induce significant O/N2 depletion, extending from the polar regions towards the equator.