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Shade nets can be an effective technology for producing bell pepper (Capsicum annuum L.) under hot climatic conditions. However, the effects of shading on fruit quality are still unclear. The objectives were to evaluate the effects of shade level on fruit mineral nutrient content, physiological disorders, and postharvest water loss. Trials were conducted in the spring–summer of 2016, 2017, and 2018 in Tifton, Georgia, USA, following a randomized complete block design with five shade levels: 0% (open field), 30%, 47%, 63%, and 80%. Shading increased the bell pepper fruit dimensions (length, diameter, and weight) in 2016 and mineral nutrient content in 2017. Fruit sunscald incidence decreased with increasing shade level, while blossom-end rot showed inconsistent responses. Postharvest fruit water loss and transpiration rates were highest in fruits from the unshaded treatment in 2016; there were no differences in fruit water loss among the shade levels. NONEXPRESSOR OF PATHOGENESIS-RELATED 1 (NPR1) and PATHOGENESIS-RELATED 1 (PR1) genes expressed more than 1.5-fold and 10-fold, respectively, at 47% shade level compared to 80%, though not significantly. Therefore, plants grown under shading had fruit with greater size, increased mineral nutrient content, and reduced sunscald incidence compared with the unshaded control.

BackgroundShoot branching is an important determinant of plant architecture and influences various aspects of growth and development. Selection on branching has also played an important role in the domestication of crop plants, including sunflower (Helianthus annuus L.). Here, we describe an investigation of the genetic basis of variation in branching in sunflower via association mapping in a diverse collection of cultivated sunflower lines.ResultsDetailed phenotypic analyses revealed extensive variation in the extent and type of branching within the focal population. After correcting for population structure and kinship, association analyses were performed using a genome-wide collection of SNPs to identify genomic regions that influence a variety of branching-related traits. This work resulted in the identification of multiple previously unidentified genomic regions that contribute to variation in branching. Genomic regions that were associated with apical and mid-apical branching were generally distinct from those associated with basal and mid-basal branching. Homologs of known branching genes from other study systems (i.e., Arabidopsis, rice, pea, and petunia) were also identified from the draft assembly of the sunflower genome and their map positions were compared to those of associations identified herein. Numerous candidate branching genes were found to map in close proximity to significant branching associations.ConclusionsIn sunflower, variation in branching is genetically complex and overall branching patterns (i.e., apical vs. basal) were found to be influenced by distinct genomic regions. Moreover, numerous candidate branching genes mapped in close proximity to significant branching associations. Although the sunflower genome exhibits localized islands of elevated linkage disequilibrium (LD), these non-random associations are known to decay rapidly elsewhere. The subset of candidate genes that co-localized with significant associations in regions of low LD represents the most promising target for future functional analyses.

SUMMARY: The composition and abundance of soluble sugars in mature pear (Pyrus) fruit are important for its acceptance by consumers. However, our understanding of the genes responsible for soluble sugar accumulation remains limited. In this study, a S1‐group member of bZIP gene family, PbrbZIP15, was characterized from pear genome through the combined analyses of metabolite and transcriptome data followed by experimental validation. PbrbZIP15, located in nucleus, was found to function in fructose, sucrose, and total soluble sugar accumulation in pear fruit and calli. After analyzing the expression profiles of sugar‐metabolism‐related genes and the distribution of cis‐acting elements in their promoters, the glucose isomerase 1 gene (PbrXylA1), whose corresponding protein catalyzed the isomerization of glucose and fructose in vitro, was identified as a downstream target gene of PbrbZIP15. PbrbZIP15 could directly bind to the G‐box element in PbrXylA1 promoter and activate its transcription, as evidenced by chromatin immunoprecipitation‐quantitative PCR, yeast one‐hybrid, electrophoretic mobility shift assay, and dual‐luciferase assay. PbrXylA1, featuring a leucine‐rich signal peptide in its N‐terminal, was localized to the endoplasmic reticulum. It was validated to play a significant role in fructose, sucrose, and total soluble sugar accumulation in pear fruit and calli, which was associated with the upregulated fructose/glucose ratio. Further studies revealed a positive correlation between the sucrose content and the expression levels of several sucrose‐biosynthesis‐related genes (PbrFRK3/8, PbrSPS1/3/4/8, and PbrSPP1) in PbrbZIP15‐/PbrXylA1‐transgenic fruit/calli. In conclusion, our results suggest that PbrbZIP15‐induced soluble sugar accumulation during pear development is at least partly attributed to the activation of PbrXylA1 transcription. Significance Statement: The composition and abundance of soluble sugars in mature pear fruit are important for its acceptance by consumers, but our understanding of the molecular mechanism underlying soluble sugar accumulation remains limited. Our findings suggest that PbrbZIP15 and its target gene PbrXylA1 participate in glucose isomerization and thus alter the composition and abundance of soluble sugars, ultimately resulting in the sweetness improvement in pear, making them potential targets for molecular breeding. [ABSTRACT FROM AUTHOR]

Background: Arbuscular mycorrhizal fungi (AMF) are beneficial soil fungi which can effectively help plants with acquisition of mineral nutrients and water and promote their growth and development. The effects of indigenous and commercial isolates of arbuscular mycorrhizal fungi on pear (Pyrus betulaefolia) trees, however, remains unclear. Methods: Trifolium repens was used to propagate indigenous AMF to simulate spore propagation in natural soils in three ways: 1. the collected soil was mixed with fine roots (R), 2. fine roots were removed from the collected soil (S), and 3. the collected soil was sterilized with 50 kGy 60Co γ-radiation (CK). To study the effects of indigenous AMF on root growth and metabolism of pear trees, CK (sterilized soil from CK in T. repens mixed with sterilized standard soil), indigenous AMF (R, soil from R in T. repens mixed with sterilized standard soil; S, soil from S in T. repens mixed with sterilized standard soil), and two commercial AMF isolates (Rhizophagus intraradices(Ri) and Funneliformis mosseae (Fm)) inoculated in the media with pear roots. Effects on plant growth, root morphology, mineral nutrient accumulation, metabolite composition and abundance, and gene expression were analyzed. Results: AMF treatment significantly increased growth performance, and altered root morphology and mineral nutrient accumulation in this study, with the S treatment displaying overall better performance. In addition, indigenous AMF and commercial AMF isolates displayed common and divergent responses on metabolite and gene expression in pear roots. Compared with CK, most types of flavones, isoflavones, and carbohydrates decreased in the AMF treatment, whereas most types of fatty acids, amino acids, glycerolipids, and glycerophospholipids increased in response to the AMF treatments. Further, the relative abundance of amino acids, flavonoids and carbohydrates displayed different trends between indigenous and commercial AMF isolates. The Fm and S treatments altered gene expression in relation to root metabolism resulting in enriched fructose and mannose metabolism (ko00051), fatty acid biosynthesis (ko00061) and flavonoid biosynthesis (ko00941). Conclusions: This study demonstrates that indigenous AMF and commercial AMF isolates elicited different effects in pear plants through divergent responses from gene transcription to metabolite accumulation. [ABSTRACT FROM AUTHOR]

Blueberry fruit are perishable after harvesting due to fruit softening, water loss and susceptibility to pathogens. Light, especially blue light, increases the accumulation of anthocyanins and reduces postharvest decay in some fruits, but the effect of blue light on postharvest fruit quality attributes in blueberries is unknown. In this study, we evaluated the effect of blue light on fruit quality, anthocyanin accumulation and disease development during postharvest cold storage (2 °C-4 °C) in two experiments with southern highbush blueberry 'Star' and rabbiteye blueberry 'Alapaha'. Overall, diurnal blue light did not affect postharvest fruit quality attributes, such as visual defects, fruit compression, skin puncture, total soluble solid content and titratable acidity, in the two cultivars compared with their respective controls (diurnal white light or continuous darkness). Further, there was no effect of blue light on fruit color and anthocyanin accumulation. Fruit disease incidence in 'Star' ranged from 19.0% to 27.3% after 21 days and in 'Alapaha' from 44.9% to 56.2% after 24 days in postharvest storage, followed by 4 days at room temperature, but blue light had no consistent effect on postharvest disease incidence for either cultivar. Disease progression following artificial inoculations with Alternaria tenuissima and Colletotrichum acutatum in 'Star' was not influenced by light treatment prior to inoculation and during fruit storage. In a separate experiment, we tested the effect of blue light on color development in 'Farthing', a southern highbush blueberry cultivar with fruit prone to non-uniform ripening, whereby the stem-end remains green as the rest of the fruit turns blue. Although green stem-end spots turned blue over time, there was no statistically significant effect of the blue light treatment. Overall, these data indicate that blue light does not affect fruit quality attributes or disease development in ripe blueberry fruit during postharvest storage in the conditions investigated here. [ABSTRACT FROM AUTHOR]