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20. Proceedings of The 28th Annual Meeting of the Japan Human Cell Society 23 August 2009 Tsukuba, Ibaraki, Japan

Author

Abei, Masato, Kameoka, Yosuke, Nakamura, Yukio, Era, Takumi, Masui, Tohru, Akaike, Toshihiro, Akutsu, Hidenori, Eto, Koji, Tamagawa, Tomoharu, Ishiwata, Isamu, Tokieda, Yuko, Iguchi, Megumi, Nakahara, Taka, Kurihara, Kunihiro, Watanabe, Yoshitaka, Kuwahata, Norihiro, Oyama, Akihiro, Ishikawa, Hiroshi, Fukushima, Tsuyoshi, Yokogami, Kiyotaka, Takeshima, Hideo, Kataoka, Hiroaki, Danjoh, Inaho, Araki, Yasuyuki, Sato, Takuya, Katagiri, Kumiko, Kubota, Yoshinobu, Araki, Yasuhisa, Ogawa, Takehiko, Ide, Yoshiaki, Tamaki, Yuichi, Tominaga, Noriko, Kawakami, Miyuki, Yasuda, Makoto, Suzuki, Minako, Nasu, Masanori, Ueda, Masatsugu, Toji, Eisaku, Yamamoto, Michiko, Kunifuji, Noriko, Nishiyama, Hiromi, Nakagawa, Tomomi, Nunobiki, Osamu, Sato, Naomi, Izuma, Shinji, Torii, Kiyo, Okamoto, Yoshiaki, Noda, Sadamu, Li, Yi, Qiang, Li, Kawaguchi, Takuya, Yamahara, Takahiro, Seno, Toshitaka, Numa, Yoshihiro, Asai, Akio, Kawamoto, Keiji, Tanaka, Nobuyuki, Miyajima, Akira, Kosaka, Takeo, Shirotake, Suguru, Hasegawa, Masanori, Kosugi, Michio, Kikuchi, Eiji, Oya, Mototsugu, Gondo, Tatsuo, Horiguchi, Yutaka, Hashimoto, Takeshi, Suzuki, Eriko, Nakashima, Jun, Umezawa, Kazuo, Tachibana, Masaaki, Tanaka, Masamitsu, Kinoshita, Manabu, Yoshihara, Yasuo, Shinomiya, Nariyoshi, Seki, Shuji, Nemoto, Koichi, Morimoto, Yuji, Nakayama, Akiyoshi, Matsuo, Hirotaka, Fujita, Masanori, Suzuki, Shinya, Ishikawa, Mayumi, Ito, Toshihide, Tsuboi, Kumiko, Tachibana, Toshiaki, Shibuya, Kazutoshi, and Yoshino, Gen

Published
2010
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38. FGF9 monomer–dimer equilibrium regulates extracellular matrix affinity and tissue diffusion

Author

Harada, Masayo, primary, Murakami, Hirotaka, additional, Okawa, Akihiko, additional, Okimoto, Noriaki, additional, Hiraoka, Shuichi, additional, Nakahara, Taka, additional, Akasaka, Ryogo, additional, Shiraishi, Yo-ichi, additional, Futatsugi, Noriyuki, additional, Mizutani-Koseki, Yoko, additional, Kuroiwa, Atsushi, additional, Shirouzu, Mikako, additional, Yokoyama, Shigeyuki, additional, Taiji, Makoto, additional, Iseki, Sachiko, additional, Ornitz, David M, additional, and Koseki, Haruhiko, additional

Published
2009
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39. Patterning of Molar Tooth Roots in Mammals

Author

S. Ota, Masato, primary, Nakahara, Taka, additional, Kanri, Yoriaki, additional, Kozawa, Yukishige, additional, Ohazama, Atsushi, additional, Aoba, Takaaki, additional, Kondo, Takashi, additional, and Iseki, Sachiko, additional

Published
2009
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46. IN SITU TISSUE ENGINEERING OF THE TRACHEA

Author

Nakamura, Tatsuo, primary, Kawanami, Eriko, additional, Fukuda, Seijunn, additional, Ueda, Hiroki, additional, Kimura, Dai, additional, Kaino, Kenji, additional, Kobayashi, Eizaburou, additional, Yoshitani, Makoto, additional, Toba, Toshinari, additional, Shimizu, Yasuhiko, additional, Kanemaru, Shinnichi, additional, Itoh, Tadao, additional, Nakahara, Taka, additional, Hori, Yoshito, additional, and Itoi, Shinnichi, additional

Published
2002
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48. Development of β-tricalcium Phosphate/Collagen Sponge Composite for Bone Regeneration.

Author

MATSUNO, Tomonori, NAKAMURA, Tatsuo, KUREMOTO, Koh-ichi, NOTAZAWA, Syunsuke, NAKAHARA, Taka, HASHIMOTO, Yoshiya, SATOH, Tazuko, and SHIMIZU, Yasuhiko

Subjects
BIOMEDICAL materials, TISSUE engineering, COLLAGEN, COMPOSITE materials, ORAL surgery, MAXILLOFACIAL surgery
Abstract

Synthetic biomaterials have been developed and used for bone grafting. Here, we developed a biodegradable sponge composite for bone tissue engineering by combining β-tricalcium phosphate (β-TCP) and collagen. In addition, we sought to determine the optimal β-TCP granules/collagen ratio by evaluating and bone formation in vivo. Porous β-TCP granules were mixed with atelocollagen hydrochloride solution at various ratios -- 0.02, 0.05, 0.1, and 0.2 g/mL. The resultant mixtures were freeze-dried and subjected to dehydrothermal treatment in vacuo. The final composites obtained were designated β-TCP/collagen sponge composites (β-TCP/CS). Through compression testing, it was found that the stress values for β-TCP/CS (0.2 g/mL) were higher than those of the other three composites over the whole strain range. Histological evaluation at four weeks after implantation revealed that the collagen sponge had degraded and newly formed bone was present on the surface of the β-TCP granules. At 12 weeks, the β-TCP granules were completely degraded and remodeling of the lamellar bone was observed. [ABSTRACT FROM AUTHOR]

Published
2006
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49. Isolation and characterization of epithelial and myogenic cells by “fishing” for the morphologically distinct cell types in rat primary periodontal ligament cultures

Author

Tominaga, Noriko, Nakahara, Taka, Nasu, Masanori, and Satoh, Tazuko

Abstract

The periodontal ligament (PDL) contains various cell populations and plays a central role in the maintenance, repair, and regeneration of the periodontium, i.e., tooth-supporting structures. Because primary cells isolated from PDL tissue are heterogeneous, the establishment of an effective isolation method for cells of interest is desired. In the present study, two morphologically distinct cell types were identified in confluent primary cultures derived from rat PDL. To isolate these cell populations, a small piece of filter paper soaked with trypsin–EDTA was placed directly onto the target cell population, enabling the cells to detach from the culture dish. The filter papers were then transferred into fresh culture dishes to establish outgrowth cultures; these two steps constitute the “cell fishing” method. The “fished” cell types were propagated and subcultured for further analyses. In morphological evaluation, immunocytochemical analyses, and reverse transcription–polymerase chain reaction, the isolated cells exhibited a polygonal appearance or a mono- or multinucleated appearance, with a high cytoplasm-to-nucleus ratio, leading to their being characterized as epithelial or myogenic cell populations, respectively. Surprisingly, a notable proportion of the multinuclear cells in the primary and subsequent isolated cultures demonstrated dramatic, spontaneous contractions, a feature typical of skeletal muscle cells. Finally, the isolated cell populations maintained a normal karyotype with a diploid chromosomal number. These results demonstrated that physiological epithelial and skeletal muscle cells can be obtained from primary PDL cultures without artificial induction using growth factors or chemicals, and can be propagated as individual lineage-committed cell populations; the populations consisted of differentiated and progenitor cells that maintained chromosomal stability. This simple, classical culture procedure provides new insights into the biological properties of PDL cells, which are potentially important for the differentiation of tissue or somatic stem cells and for the development of future cell-based therapies for dental and muscular diseases.

Published
2013
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50. In Situ Tissue Engineering of Periodontal Tissues by Seeding with Periodontal Ligament-Derived Cells

Author

Nakahara, Taka, Nakamura, Tatsuo, Kobayashi, Eizaburo, Kuremoto, Koh-Ichi, Matsuno, Tomonori, Tabata, Yasuhiko, Eto, Kazuhiro, and Shimizu, Yasuhiko

Abstract

The feasibility of an in situ tissue-engineering method employing cell-based therapy with autologous periodontal ligament-derived cells was investigated. Periodontal ligament cells were obtained from six beagle dogs. Periodontal fenestration defects (6 × 4 mm) were created bilaterally at a location 6 mm apical to the marginal alveolar crest in the maxillary canines. Alkaline phosphatase-positive periodontal ligament cells (3 × 105 cells) were seeded onto a collagen sponge scaffold just before implantation. One defect was filled with the cell-scaffold construct, and another was left empty as the control. All animals were killed 4 weeks after surgery, and specimens were evaluated histomorphometrically. All the histomorphometrical data were analyzed by three-way analysis of variance with the Bonferroni multiple comparisons test. Regeneration of apical tissue was faster than that of coronal and isolated tissues on the control side (apical > coronal > isolated; p < 0.0001). On the other hand, on the cell-seeded side, regeneration of the cementum was observed uniformly on the root surface. Our data suggest that the seeded cells induced cementum regeneration on the root surface, indicating the potential of in situ tissue engineering using autologous cells for the regeneration of periodontal tissues.

Published
2004
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