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Covid-19 has spread globally and causes severe acute respiratory syndrome. The symptoms of covid-19 have similarities with influenza, such as cough, fever, runny nose, and sore throat. Therefore, the internet sources tend to have an increasing search related to influenza symptoms drugs. This study aims to assess the search trend of influenza symptoms drugs using google trend analysis in Indonesia. We explore google trend analysis using search terms in the Indonesian language related to influenza symptoms drugs from December 6th, 2020 to November 30th, 2021. The positive confirmed cases were obtained from the Indonesian government website https://covid19.go.id/. Our results demonstrated the increasing search terms related to influenza drug symptoms during July and August. The highest term search was “obat batuk”. The positive covid-19 confirmed cases in Indonesia increased during July and August. During the peak of the covid-19 outbreak in Indonesia in July-August 2021, there was an increase in google trends searching related to influenza’s drug symptoms.

Heart failure is a progressive clinical syndrome that results in structural or functional abnormalities of the heart that disrupt the heart’s performance in pumping blood. The clinical manifestations are shortness of breath and cardiomegaly. The purpose of the study was to determine the Lisinopril therapy (dose, route of administration, frequency) in heart failure patients at the hospitalized installation of Sidoarjo Hospital. The method of collecting the data was retrospectively by health medical records at Sidoarjo Hospital from January 1 until December 31, 2020. The results showed that the health medical records sample got inclusion criteria of 20 patients. Heart failure was suffered by 12 patients (60%) male patients and 8 female patients (40%). The highest age range was 56- 65 years, with 8 patients (40%). In patients with the pattern of using combination 2, there were 9 patients (33%), combination 3 had 9 patients (33%), and combination 4 had 9 patients (33%). The highest combination of 2 was Lisinopril (1x 10 mg) po + Bisoprolol (1x 2.5 mg) po. There were 2 patients (22%). The highest combination of 3 was Lisinopril (1x5 mg) po + Bisoprolol (1x 2.5 mg) po + Furosemide (1 x 20 mg) iv. There were 2 patients (22%). The highest combination of 4 was Lisinopril (1x 10 mg) po + Bisoprolol (1x 2.5 mg) po + Furosemide (3x 40 mg) iv + Spironolactone (1x 25 mg) po. There were 3 patients (33%). The results show that there are 16 patterns of using switch therapy. Keywords: lisinopril, heart failure, hospitalization

Inadequate levels of 5-methyltetrahydrofolate (5-MTHF) and the T variant of MTHFR C677T have been suggested to be associated with an increased risk of developing mental illness, whereas the PON1 SNP variant provides a protective role. However, reports validating the methodology for plasma 5-MTHF levels in schizophrenia patients are limited. A sensitive LC–MS/MS system using an amide column and calibration curve was determined by dialyzed human plasma, and applied to schizophrenia patients and healthy controls in Taiwan, and the differences between the subgroups were discussed. This analysis system meets regulation criteria, and the lower limit of quantification for 5-MTHF levels was 4 nM from 200 μL plasma, within 7 min. The mean plasma 5-MTHF levels in schizophrenia patients (n = 34; 11.70 ± 10.37 nM) were lower than those in the healthy controls (n = 42; 22.67 ± 11.12 nM) significantly (p < 0.01). 5-MTHF concentrations were significantly lower in male carriers than in female carriers (18.30 ± 10.37 nM vs. 24.83 ± 11.01 nM, p < 0.05), especially in subjects who were MTHFR CT/PON1 Q allele carriers. In conclusion, this quantitative system, which employed sensitive and simple processing methods, was successfully applied, and identified that schizophrenic patients had significantly lower levels of 5-MTHF. Lower plasma 5-MTHF concentrations were observed in male subjects.

Protein phosphorylation can induce signal transduction to change sperm motility patterns during sperm capacitation. However, changes in the phosphorylation of sperm proteins in mice are still incompletely understood. Here, capacitation-related phosphorylation in mouse sperms were firstly investigated by label-free quantitative (LFQ) phosphoproteomics coupled with bioinformatics analysis using ingenuity pathway analysis (IPA) methods such as canonical pathway, upstream regulator, and network analysis. Among 1632 phosphopeptides identified at serine, threonine, and tyrosine residues, 1050 novel phosphosites, corresponding to 402 proteins, were reported. Gene heatmaps for IPA canonical pathways showed a novel role for GSK-3 in GP6 signaling pathways associated with capacitation for 60 min. At the same time, the reduction of the abundant isoform-specific GSK-3&alpha, expression was shown by western blot (WB) while the LFQ pY of this isoform slightly decreased and then increased. The combined results from WB and LFQ methods explain the less inhibitory phosphorylation of GSK-3&alpha, during capacitation and also support the predicted increases in its activity. In addition, pAKAP4 increased at the Y156 site but decreased at the Y811 site in a capacitated state, even though IPA network analysis and WB analysis for overall pAKAP revealed upregulated trends. The potential roles of GSK-3 and AKAP4 in fertility are discussed.

Additional file 2: Supplementary Information.

Stroke iskemik adalah sindrom yang berupa gejala hilangnya fungsi sistem saraf pusat yang berkembang pesat dapat karena atherothrombotis atau emboli, yang keduanya dapat mengganggu aliran darah serebral (CBF). Pemilihan terapi antihipertensi yang sesuai pada pasien stroke sangat berpengaruh dalam keberhasilan terapi yang dilakukan. Diuretik efektif untuk mengurangi tekanan intrakranial. Penelitian ini bertujuan untuk mengetahui pola penggunaan obat diuretik pada pasien stroke iskemik di RSU Dr Saiful Anwar Malang dan meninjau hubungan terapeutik terkait jenis obat, dosis, cara pemberian, interval, frekuensi, dan durasi dengan data klinis dan data laboratorium pasien. Penelitian ini merupakan penelitian observasional dan data dikumpulkan secara retrospektif pada pasien stroke iskemik dari 1 Juli hingga 31 Desember 2012. Profil diuretik tunggal yang digunakan adalah mannitol. Kombinasi diuretik yang ditemukan adalah furosemid dan spironolacton. Penggunaan terapi diuretik pada pasien dengan stroke iskemik sudah sesuai dengan pedoman. Kata kunci: Diuretik, stroke iskemik, rawat inap .

Cultural studies about treatment methods and herbal plants in an ethnic important to understand in order to know the different methods of treatment and medicinal plants used from each ethnicity. In addition, this study is also useful to know the drugs from natural ingredients that have not been identified. In this study, as the object of research is ethnic Using, Kemiren village, District Glagah Banyuwangi. The method used in this study include interviews with local community and the people who play a role in traditional medicine in these ethnic. The results obtained information on 62 commonly used medicinal plants for traditional medicine. From 62 plants were then performed classification based on the bioactivity.