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This study aims to develop, characterize, and evaluate the best statin-loaded Collagen Silver nanoparticle-coated polycaprolactone nanofibers by electrospinning technique. The characterization and evaluation results reveal that nanofibers had shown an aligned Collagen Silver nanoparticle-coated polycaprolactone nanofibers distribution of fibers and aliment of collagen and silver nanoparticle coating material on the surface of the nanofibers. Furthermore, the electrospun nanofibers have shown no interaction between the drug and collagen and PCL polymeric material and have demonstrated controlled drug release for 360 h and significant degradation for 60 days of the study. In vitro, among the nanofiber treatment groups and the control groups, Pravastatin Collagen Silver nanoparticle-coated polycaprolactone nanofibers have shown cell proliferation and migration of NHDF cells and blood vessel development in the CAM assay and potential anti-bacterial activity against S. aureus and E. coli. In vivo, the Pravastatin Collagen Silver nanoparticle-coated polycaprolactone nanofibers have demonstrated improved wound recovery, reepithelization, fibroblast cell proliferation, angiogenesis, and ECM remodeling and enhanced VEGF-sprouting in the excised tissues of streptozotocin-induced diabetic rats for 7, 14, and 21 days of the study. Therefore, the developed Pravastatin Collagen Silver nanoparticle-coated polycaprolactone nanofibers could be a promising wound-dressing platform for effectively treating and managing diabetic wounds. Highlights * Nanofiber wound dressings provide numerous structural features in biomaterials. * Polycaprolactone can release the repurposed statin molecules into the wound site in a controlled manner. * In diabetic wounds, ECM tissue remodeling stimulates repair and vessel growth. * Pravastatin has been demonstrated to improve angiogenesis and ECM secretion for wound healing in diabetic conditions. KEYWORDS diabetic wounds, electrospinning, hypoxic microenvironment, nanofibers, pro-angiogenesis, re-epithelization, wound dressing, 1 | INTRODUCTION The most prevalent cause of death in diabetic individuals is diabetic wounds. Unfortunately, the bulk of the treatment options in the market do not entirely address how [...]

Background: Karkataka Taila (KT) is a virgin coconut oil (VCO) based Rasayana formulation that is enriched with the flesh of freshwater edible crab, Scylla serrata, used to treat Parkinson's Disease (PD) or Kampavata by local Ayurveda practitioners of Kerala state. There is no scientific study carried out on its toxicological effects so far. Objective: To understand the ayurvedic preparation method for KT and assessment of the toxicological effects of the KT and VCO on SH-SY5Y cell lines and Drosophila melanogaster embryos. Materials and methods: The SH-SY5Y cell lines treated with different concentrations of KT and VCO range from 6.25 µg/ml to 100 µg/ml and Drosophila melanogaster embryos fed with food containing different concentrations of KT and VCO, ranging from 0.005 % to 10 %. Results: KT and VCO did not show any significant cytotoxicity effect on SH-SY5Y cell lines up to a dose concentration of 25 µg. But, at 50 µg and 100 µg concentrations, KT has shown a cytotoxic effect and it was higher than the VCO. The toxicological analysis in Drosophila has shown that the survival rate of the KT treated group at concentration ranges from 0.005 % to 10 % is significantly decreased from 78.8 % to 27.7 %, compared to the control group, whereas in VCO treated group, at 0.005 % to 10 %, the survival rate has decreased from 76.2 % to 66 %, which is marginally higher than the KT treated group. Conclusion: Our findings revealed that as the concentration of Rasayana in the medium increases, there is a noticeable adverse effect on the percentage viability in SH-SY5Y cell lines and in the number of offspring in Drosophila. The effect of vehicle, VCO, at the same concentration has shown a protective effect on cell lines and flies. It can be concluded that the toxic effect has been observed only at higher concentrations of KT and at the lower concentration, the toxic effect has been minimal. [ABSTRACT FROM AUTHOR]

'Karkataka Taila (KT), an ancient Ayurvedic Rasayana comprising the edible freshwater crab Scylla serrata Forskal flesh, is still used by local traditional practitioners in Kerala state to treat tremors and palsy. In the scientific community, it becomes less exposed due to the lack of adequate scientific validations and brief reports. There has been no published research on the effectiveness of KT in treating Parkinson's disease (PD). The purpose of the current research work was to investigate the anti-Parkison's potential of KT against rotenone-induced neurotoxicity in SH-SY5Y cell lines and rat model of PD and investigate underlying molecular mechanisms. The components of KT have been identified by gas chromatography-mass spectroscopy (GC-MS). The neuroprotective activity of KT was assessed using SH-SY5Y cell lines and rats against rotenone-induced PD. The parameters used for asses the neuroprotection are antioxidant markers (ROS and SOD), anti-inflammatory markers (IL-6, IL-1β, TNF-α, and nitrite), and dopamine levels. Behavioral evaluation and rat brain histopathology were carried out to further support the neuroprotection. Analysis using GC-MS revealed 36 constituents in KT. In vitro , the KT displayed considerable neuroprotective effects in terms of decreasing oxidative stress (ROS and SOD), neuroinflammation (IL-6, IL-1β, TNF-α, and nitrite), and elevating dopamine concentration. In vivo data showing improvements in histopathological and biochemical parameters confirmed the in vitro study findings, and in terms of behavioral assays, KT displayed significant activity. GC-MS profiling was used to identify the bioactive compounds of KT with antioxidant, anti-inflammatory, and neuroprotective properties. As a result, they may be responsible for the therapeutic effects of KT on PD. [Display omitted] [ABSTRACT FROM AUTHOR]