Search

Your search keyword '"Nagy, Gabe"' showing total 175 results
Start Over Author "Nagy, Gabe"
175 results on '"Nagy, Gabe"'

5. Recent advances in high‐resolution traveling wave‐based ion mobility separations coupled to mass spectrometry.

Author

Naylor, Cameron N. and Nagy, Gabe

Subjects
*ION mobility, *INFRARED spectroscopy, *IONIC mobility, *ION acoustic waves, *IONIC structure
Abstract

Recently, ion mobility spectrometry‐mass spectrometry (IMS‐MS) has become more readily incorporated into various omics‐based workflows. These growing applications are due to developments in instrumentation within the last decade that have enabled higher‐resolution ion mobility separations. Two such platforms are the cyclic (cIMS) and structures for lossless ion manipulations (SLIM), both of which use traveling wave ion mobility spectrometry (TWIMS). High‐resolution separations achieved with these techniques stem from the drastically increased pathlengths, on the order of 10 s of meters to >1 km, in both cIMS‐MS and SLIM IMS‐MS, respectively. Herein, we highlight recent developments and advances, for the period 2019–2023, in high‐resolution traveling wave‐based IMS‐MS through instrumentation, calibration strategies, hyphenated techniques, and applications. Specifically, we will discuss applications including CCS calculations in multipass IMS‐MS separations, coupling of IMS‐MS with chromatography, imaging, and cryogenic infrared spectroscopy, and isomeric separations of glycans, lipids, and other small metabolites. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

6. Development of a cyclic ion mobility spectrometry‐mass spectrometry‐based collision cross‐section database of permethylated human milk oligosaccharides.

Author

Habibi, Sanaz C., Bradford, Victoria R., Baird, Sophie C., Lucas, Shadrack Wilson, Chouinard, Christopher D., and Nagy, Gabe

Subjects
LIQUID chromatography-mass spectrometry, ION mobility, MASS spectrometry, BREAST milk, IONIC mobility, ION mobility spectroscopy
Abstract

Human milk oligosaccharides (HMOs) are an important class of biomolecules responsible for the healthy development of the brain‐gut axis of infants. Unfortunately, their accurate characterization is largely precluded due to a variety of reasons – there are over 200 possible HMO structures whereas only 10s of these are available as authentic analytical standards. Furthermore, their isomeric heterogeneity stemming from their many possible glycosidic linkage positions and corresponding α/β anomericities further complicates their analyses. While liquid chromatography coupled to tandem mass spectrometry remains the gold standard for HMO analyses, it often times cannot resolve all possible isomeric species and thus warrants the development of other orthogonal approaches. High‐resolution ion mobility spectrometry coupled to mass spectrometry has emerged as a rapid alternative to condensed‐phase separations but largely has remained limited to qualitative information related to the resolution of isomers. In this work, we have assessed the use of permethylation to improve both the resolution and sensitivity of HMO analyses with cyclic ion mobility separations coupled with mass spectrometry. In addition to this, we have developed the first‐ever high‐resolution collision cross‐section database for permethylated HMOs using our previously established calibration protocol. We envision that this internal reference database generated from high‐resolution cyclic ion mobility spectrometry‐mass spectrometry will greatly aid in the accurate characterization of HMOs and provide a valuable, orthogonal, approach to existing liquid chromatography–tandem mass spectrometry‐based methods. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

12. Non-threaded isomers of sungsanpin and ulleungdin lasso peptides inhibit H1299 cancer cell migration

Author

Digal, Lori, primary, Samson, Shiela, additional, Stevens, Mark, additional, Ghorai, Abhijit, additional, Kim, Hyungyu, additional, Mifflin, Marcus, additional, Carney, Keith, additional, Williamson, David, additional, Um, Soohyun, additional, Nagy, Gabe, additional, Oh, Dong-Chan, additional, Mendoza, Michelle, additional, and Roberts, Andrew, additional

Published
2023
Full Text
View/download PDF

17. Nonthreaded Isomers of Sungsanpin and Ulleungdin Lasso Peptides Inhibit H1299 Cancer Cell Migration

Author

Digal, Lori, Samson, Shiela C., Stevens, Mark A., Ghorai, Abhijit, Kim, Hyungyu, Mifflin, Marcus C., Carney, Keith R., Williamson, David L., Um, Soohyun, Nagy, Gabe, Oh, Dong-Chan, Mendoza, Michelle C., and Roberts, Andrew G.

Abstract

Lasso peptides are a structurally distinct class of biologically active natural products defined by their short sequences with impressively interlocked tertiary structures. Their characteristic peptide [1]rotaxane motif confers marked proteolytic and thermal resiliency, and reports on their diverse biological functions have been credited to their exceptional sequence variability. Because of these unique properties, taken together with improved technologies for their biosynthetic production, lasso peptides are emerging as a designable scaffold for peptide-based therapeutic discovery and development. Although the defined structure of lasso peptides is recognized for its remarkable properties, the role of the motif in imparting bioactivity is less understood. For example, sungsanpin and ulleungdin are natural lasso peptides that similarly exhibit encouraging cell migration inhibitory activities in A549 lung carcinoma epithelial cells, despite sharing only one-third of the sequence homology. We hypothesized that the shape of the lasso motif is beneficial for the preorganization of the conserved residues, which might be partially retained in variants lacking the threaded structure. Herein, we describe solid-phase peptide synthesis strategies to prepare acyclic, head-to-side chain (branched), and head-to-tail (macrocyclic) cyclic variants based on the sungsanpin (Sun) and ulleungdin (Uln) sequences. Proliferation assays and time-lapse cell motility imaging studies were used to evaluate the cell inhibitory properties of natural Sun compared with the synthetic Sun and Uln isomers. These studies demonstrate that the lasso motif is not a required feature to slow cancer cell migration and more generally show that these nonthreaded isomers can retain similar activity to the natural lasso peptide despite the differences in their overall structures.

Published
2024
Full Text
View/download PDF

22. Implementation of Ion Mobility Spectrometry-Based Separations in Structures for Lossless Ion Manipulations (SLIM)

Author

Hollerbach, Adam, Conant, Christopher R., Nagy, Gabe, and Ibrahim, Yehia M.

Subjects
Ions, Isomerism, Ion Mobility Spectrometry, Microelectrodes, Article, Mass Spectrometry
Abstract

Structures for Lossless Ion Manipulations (SLIM) is a powerful variant of traveling wave ion mobility spectrometry (TW-IMS) that uses a serpentine pattern of microelectrodes deposited onto printed circuit boards to achieve ultralong ion path lengths (13.5 m). Ions are propelled through SLIM platforms via arrays of TW electrodes while RF and DC electrodes provide radial confinement, establishing near lossless transmission. The recent ability to cycle ions multiple times through a SLIM has allowed ion path lengths to exceed 1000 m, providing unprecedented separation power and the ability to observe ion structural conformations unobtainable with other IMS technologies. The combination of high separation power, high signal intensity, and the ability to couple with mass spectrometry places SLIM in the unique position of being able to address longstanding proteomics and metabolomics challenges by allowing the characterization of isomeric mixtures containing low abundance analytes.

Published
2022

24. Measurement and Theory of Gas-Phase Ion Mobility Shifts Resulting from Isotopomer Mass Distribution Changes

Author

Harrilal, Christopher P., primary, Gandhi, Viraj D., additional, Nagy, Gabe, additional, Chen, Xi, additional, Buchanan, Michael G., additional, Wojcik, Roza, additional, Conant, Christopher R., additional, Donor, Micah T., additional, Ibrahim, Yehia M., additional, Garimella, Sandilya V. B., additional, Smith, Richard D., additional, and Larriba-Andaluz, Carlos, additional

Published
2021
Full Text
View/download PDF

27. Implementation of charged microdroplet-based derivatization of bile acids on a cyclic ion mobility spectrometry-mass spectrometry platformElectronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d3ay01447a

Author

Rciš, Olavs and Nagy, Gabe

Abstract

Herein, we report the first implementation of charged microdroplet-based derivatization on a commercially-available cyclic ion mobility spectrometry-mass spectrometry platform. We have demonstrated the potential of our approach to improve separability of challenging isomers, but more importantly to rapidly screen derivatization reactions through droplet chemistry. Additionally, the use of cyclic ion mobility separations and tandem mass spectrometry reveals insights into product formation that would be lost with single stage mass spectrometry. Overall, we anticipate broad utility of our methodology owing to the simple design and setup for performing these droplet-based reactions and future work coupling these reactions online with liquid chromatography.

Published
2023
Full Text
View/download PDF

28. Dynamic Time-Warping Correction for Shifts in Ultrahigh Resolving Power Ion Mobility Spectrometry and Structures for Lossless Ion Manipulations

Author

Hollerbach, Adam L., primary, Conant, Christopher R., additional, Nagy, Gabe, additional, Monroe, Matthew E., additional, Gupta, Khushboo, additional, Donor, Micah, additional, Giberson, Cameron M., additional, Garimella, Sandilya V. B., additional, Smith, Richard D., additional, and Ibrahim, Yehia M., additional

Published
2021
Full Text
View/download PDF

30. Assessing Collision Cross Section Calibration Strategies for Traveling Wave-Based Ion Mobility Separations in Structures for Lossless Ion Manipulations

Author

Li, Ailin, primary, Conant, Christopher R., additional, Zheng, Xueyun, additional, Bloodsworth, Kent J., additional, Orton, Daniel J., additional, Garimella, Sandilya V.B., additional, Attah, Isaac K., additional, Nagy, Gabe, additional, Smith, Richard D., additional, and Ibrahim, Yehia M., additional

Published
2020
Full Text
View/download PDF

32. Ion Mobility Spectrometry with High Ion Utilization Efficiency Using Traveling Wave-Based Structures for Lossless Ion Manipulations

Author

Li, Ailin, primary, Nagy, Gabe, additional, Conant, Christopher R., additional, Norheim, Randolph V., additional, Lee, Joon Yong, additional, Giberson, Cameron, additional, Hollerbach, Adam L., additional, Prabhakaran, Venkateshkumar, additional, Attah, Isaac K., additional, Chouinard, Christopher D., additional, Prabhakaran, Aneesh, additional, Smith, Richard D., additional, Ibrahim, Yehia M., additional, and Garimella, Sandilya V.B., additional

Published
2020
Full Text
View/download PDF

33. Ultra-High-Resolution Ion Mobility Separations Over Extended Path Lengths and Mobility Ranges Achieved using a Multilevel Structures for Lossless Ion Manipulations Module

Author

Hollerbach, Adam L., primary, Li, Ailin, additional, Prabhakaran, Aneesh, additional, Nagy, Gabe, additional, Harrilal, Christopher P., additional, Conant, Christopher R., additional, Norheim, Randolph V., additional, Schimelfenig, Colby E., additional, Anderson, Gordon A., additional, Garimella, Sandilya V. B., additional, Smith, Richard D., additional, and Ibrahim, Yehia M., additional

Published
2020
Full Text
View/download PDF

34. Rapid and Simultaneous Characterization of Drug Conjugation in Heavy and Light Chains of a Monoclonal Antibody Revealed by High-Resolution Ion Mobility Separations in SLIM

Author

Nagy, Gabe, primary, Attah, Isaac K., additional, Conant, Christopher R., additional, Liu, Weijing, additional, Garimella, Sandilya V.B., additional, Gunawardena, Harsha P., additional, Shaw, Jared B., additional, Smith, Richard D., additional, and Ibrahim, Yehia M., additional

Published
2020
Full Text
View/download PDF

37. SLIM Ultrahigh Resolution Ion Mobility Spectrometry Separations of Isotopologues and Isotopomers Reveal Mobility Shifts due to Mass Distribution Changes

Author

Wojcik, Roza, primary, Nagy, Gabe, additional, Attah, Isaac. K., additional, Webb, Ian K., additional, Garimella, Sandilya V. B., additional, Weitz, Karl K., additional, Hollerbach, Adam, additional, Monroe, Matthew E., additional, Ligare, Marshall R., additional, Nielson, Felicity F., additional, Norheim, Randolph V., additional, Renslow, Ryan S., additional, Metz, Thomas O., additional, Ibrahim, Yehia M., additional, and Smith, Richard D., additional

Published
2019
Full Text
View/download PDF

44. Isolation of Tryptanthrin and Reassessment of Evidence for Its Isobaric Isostere Wrightiadione in Plants of the Wrightia Genus

Author

Garcellano, Rhea C., primary, Moinuddin, Syed G. A., additional, Young, Robert P., additional, Zhou, Mowei, additional, Bowden, Mark E., additional, Renslow, Ryan S., additional, Yesiltepe, Yasemin, additional, Thomas, Dennis G., additional, Colby, Sean M., additional, Chouinard, Christopher D., additional, Nagy, Gabe, additional, Attah, Isaac K., additional, Ibrahim, Yehia M., additional, Ma, Rui, additional, Franzblau, Scott G., additional, Lewis, Norman G., additional, Aguinaldo, Alicia M., additional, and Cort, John R., additional

Published
2018
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results