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16 results on '"Nagpal, Sonal"'

7. Metabolic and lipidomic profiling of steatotic human livers during ex situ normothermic machine perfusion guides resuscitation strategies

Author

Raigani, Siavash, primary, Karimian, Negin, additional, Huang, Viola, additional, Zhang, Anna M., additional, Beijert, Irene, additional, Geerts, Sharon, additional, Nagpal, Sonal, additional, Hafiz, Ehab O. A., additional, Fontan, Fermin M., additional, Aburawi, Mohamed M., additional, Mahboub, Paria, additional, Markmann, James F., additional, Porte, Robert J., additional, Uygun, Korkut, additional, Yarmush, Martin, additional, and Yeh, Heidi, additional

Published
2020
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8. Supercooling Of Human Livers To Extend The Preservation Time For Transplantation

Author

De Vries, Reinier, primary, Tessier, Shannon, additional, Banik, Peony, additional, Nagpal, Sonal, additional, Cronin, Stephanie, additional, Ozer, Sinan, additional, Hafiz, Ehab, additional, Van Gulik, Thomas, additional, Yarmush, Martin, additional, Markmann, James, additional, Toner, Mehmet, additional, Yeh, Heidi, additional, and Uygun, Korkut, additional

Published
2019
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9. Partial Freezing: A New Integrated Approach To Organ Banking

Author

Tessier, Shannon, primary, Cronin, Stephanie, additional, Pendexter, Casie, additional, De Vries, Reinier, additional, Kimura, Shoko, additional, Ozer, Sinan, additional, Yeh, Heidi, additional, Uygun, Korkut, additional, Toner, Mehmet, additional, Banik, Peony, additional, and Nagpal, Sonal, additional

Published
2019
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10. Bulk Droplet Vitrification Of Primary Hepatocytes

Author

De Vries, Reinier, primary, Banik, Peony, additional, Nagpal, Sonal, additional, Weng, Lindong, additional, Ozer, Sinan, additional, Van Gulik, Thomas, additional, Toner, Mehmet, additional, Tessier, Shannon, additional, and Uygun, Korkut, additional

Published
2019
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12. Subnormothermic Machine Perfusion of Steatotic Livers Results in Increased Energy Charge at the Cost of Anti-Oxidant Capacity Compared to Normothermic Perfusion

Author

Karimian, Negin, primary, Raigani, Siavash, additional, Huang, Viola, additional, Nagpal, Sonal, additional, Hafiz, Ehab O. A., additional, Beijert, Irene, additional, Mahboub, Paria, additional, Porte, Robert J., additional, Uygun, Korkut, additional, Yarmush, Martin, additional, and Yeh, Heidi, additional

Published
2019
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16. Bulk Droplet Vitrification: An Approach to Improve Large-Scale Hepatocyte Cryopreservation Outcome

Author

de Vries, Reinier J., Banik, Peony D., Nagpal, Sonal, Weng, Lindong, Ozer, Sinan, van Gulik, Thomas M., Toner, Mehmet, Tessier, Shannon N., and Uygun, Korkut

Abstract

Loss of hepatocyte viability and metabolic function after cryopreservation is still a major issue. Although vitrification is a promising alternative, it has generally been proven to be unsuitable for vitrification of large cell volumes which is required for clinical applications. Here, we propose a novel bulk droplet (3–5 mm diameter) vitrification method which allows high throughput volumes (4 mL/min), while using a low preincubated CPA concentration (15% v/v) to minimize toxicity and loss of cell viability and function. We used rapid (1.25 s) osmotic dehydration to concentrate a low preincubated intracellular CPA concentration ahead of vitrification, without the need of fully equilibrating toxic CPA concentrations. We compared direct postpreservation viability, long-term viability, and metabolic function of bulk droplet vitrified, cryopreserved, and fresh hepatocytes. Simulations and cooling rate measurements confirmed an adequate concentration of the intracellular CPA concentration (up to 8.53 M) after dehydration in combination with high cooling rates (960–1320 °C/min) for successful vitrification. In comparison to cryopreserved hepatocytes, bulk droplet vitrified hepatocytes had a significantly higher viability, directly after preservation and after 1 day in culture. Moreover, bulk droplet vitrified hepatocytes had evidently better morphology and showed significantly higher metabolic activity than cryopreserved hepatocytes in long-term collagen sandwich cultures. In conclusion, we developed a novel bulk droplet vitrification method of which we validated the theoretical background and demonstrated the feasibility to use this method to vitrify large cell volumes. Moreover, we showed that this method results in improved hepatocyte viability and metabolic function as compared to cryopreservation.

Published
2019
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