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Aim: To evaluate and compare the antimicrobial efficacy of Ca(OH) 2 , 25% propolis, and 25% Glycyrrhiza glabra as intracanal medicaments in root canal treatment., Materials and Methods: Total 60 freshly extracted permanent incisors were decoronated and chemomechanical preparation of root canal was performed. Samples were inoculated with a pure culture of Enterococcus faecalis and incubated for 21 days. Colony-forming units (CFUs) were recorded before medication. Incubated samples were randomly categorized into three groups, namely, Ca(OH) 2 , propolis, and G. glabra , with 20 samples in each group. Antibacterial activity was assessed by evaluating the variance in the CFUs on Day 7. Paired " t " test and Post-hoc Tukey's test were applied to analyze the data., Results: Reduction of CFUs was noticed in all the groups ( p <0.001), however the reduction was more predominant in the propolis group., Conclusion: Propolis is more effective against E. faecalis , when compared to G. glabra and Ca(OH) 2 ., Clinical Significance: Propolis could be used as an effective medicament in root canal treatment.

Purpose: New Competency-Based Medical Education (CBME) curriculum has emphasized on the acquisition of core competencies by an Indian Medical Graduate (IMG). Likewise ability to perform basic microbiological investigations, and diagnose infectious disease is deemed critical for a doctor of first contact. In order to prepare students to achieve these competencies, effective skill training and assessment is paramount. However, microbiology skill training is known to vary across Indian medical schools. This survey has explored faculty perceptions and current practices across the country, to suggest measures for strengthening skill acquisition., Methods: Online survey was conducted through Google form questionnaire. Faculty shared their perceptions and practices on the Likert scale about teaching, learning and assessment of microbiology skills., Results: Sixty faculty members from 58 medical colleges from 17 states of India participated. Majority of the faculty considered interpretational skills as more important than technical skills. Faculty perceptions and practices varied. Although most of the skills are being taught and assessed on one or more occasions, some important clinical microbiology skills were not assessed. Feasibility was an issue for new set of CBME competencies. Blue print and skill laboratory was adopted by 10% and 31.66% colleges respectively., Conclusions: Variation in perceptions and practices in teaching and assessment of microbiology skills in Indian context is now documented. Skill training requires a standardised and robust program with ample opportunities for practice and feedback. Faculty orientation and use of innovative strategies are overriding to augment skill acquisition and thus, successful implementation of new CBME curriculum., Competing Interests: Declaration of competing interest Nil., (Copyright © 2021 Indian Association of Medical Microbiologists. Published by Elsevier B.V. All rights reserved.)

Background and Objectives: The mitochondrial electron transport chain (mtETC) of Plasmodium falciparum is an important drug target. Identification and functional validation of putative mitochondrial proteins of the mtETC is critical for drug development. Many of the regulatory subunits and assembly factors of cytochrome c oxidase readily identifiable in humans and yeast are missing in P. falciparum. Here, we describe our efforts to identify and validate the function of putative Pfsurf1, a key assembly factor of complex IV of the mtETC., Methods: Multiple sequence alignment of SURF 1/Shy 1 was carried out in Clustal X 2.1. Phylogenetic tree was constructed using "Draw tree" option in Clustal X, and was analyzed using interactive Tree of Life software. To identify the conserved sequences, domain search was done using Jalview version 2.8.2 (BLOSUM 62 scoring). The haploid Saccharomyces cerevisiae strain (BY4741) containing the null allele shy1 (Orf: YGR112w) (shy1::Kan) was complemented with putative Pfsurf1 to study its ability to rescue the growth defect., Results: Similarity searches of PfSURF1-like protein in the Pfam shows statistically significant E = 4.7e-10 match to SURF1 family. Sequence alignment of PfSURF1 with other SURF1-like proteins reveals the conservation of transmembrane domains, α-helices and β-pleated sheets. Phylogenetic analysis clusters putative PfSURF1 with apicomplexan SURF1-like proteins. Yeast complementation studies show that Pfsurf1 can partially rescue the yeast shy1 mutant, YGR112w., Interpretation & Conclusion: Bioinformatics and complementation studies in yeast show that P. falciparum's SURF1 is the functional ortholog of human SURF1 and yeast Shy1., Competing Interests: None

Prohibitins (PHBs) are evolutionarily conserved mitochondrial integral membrane proteins, shown to regulate mitochondrial structure and function, and can be classified into PHB1 and PHB2. PHB1 and PHB2 have been shown to interact with each other, and form heterodimers in mitochondrial inner membrane. Plasmodium falciparum has orthologues of PHB1 and PHB2 in its genome, and their role is unclear. Here, by homology modelling and yeast two-hybrid analysis, we show that putative Plasmodium PHBs (Pf PHB1 and Pf PHB2) interact with each other, which suggests that they could form supercomplexes of heterodimers in Plasmodium, the functional form required for optimum mitochondrial function., Competing Interests: None

Purpose: PowerPoint (PPT™) presentation has become an integral part of day-to-day teaching in medicine. Most often, PPT™ is used in its default mode which in fact, is known to cause boredom and ineffective learning. Research has shown improved short-term memory by applying multimedia principles for designing and delivering lectures. However, such evidence in medical education is scarce. Therefore, we attempted to evaluate the effect of multimedia principles on enhanced learning of parasitology., Methodology: Second-year medical students received a series of lectures, half of the lectures used traditionally designed PPT™ and the rest used slides designed by Mayer's multimedia principles. Students answered pre and post-tests at the end of each lecture (test-I) and an essay test after six months (test-II) which assessed their short and long term knowledge retention respectively. Students' feedback on quality and content of lectures were collected., Results: Statistically significant difference was found between post test scores of traditional and modified lectures (P = 0.019) indicating, improved short-term memory after modified lectures. Similarly, students scored better in test II on the contents learnt through modified lectures indicating, enhanced comprehension and improved long-term memory (P < 0.001). Many students appreciated learning through multimedia designed PPT™ and suggested for their continued use., Conclusions: It is time to depart from default PPT™ and adopt multimedia principles to enhance comprehension and improve short and long term knowledge retention. Further, medical educators may be trained and encouraged to apply multimedia principles for designing and delivering effective lectures.

Background . A Nugent score > 7 has been defined as the gold standard for the diagnosis for bacterial vaginosis (BV), though it is resource intensive and impractical as point of care testing. We sought to determine if colorimetric assessment of vaginal pH can accurately predict the occurrence of BV. Methods . We performed a planned subanalysis of 1,216 pregnant women between 13 0/7 and 19 6/7 weeks who underwent vaginal examination as part of a randomized controlled trial. Using a standardized technique, specimens were obtained for colorimetric assessment and two separate slides for Gram staining. These slides were subsequently evaluated by two independent blinded microbiologists for Nugent scoring. Results . Interrater reliability of the interpretation of the Nugent score was excellent (intraclass correlation-individual 0.93 (95 CI 0.92 to 0.94) and average 0.96 (95% CI 0.95 to 0.97)). The sensitivity of an elevated pH > 5 for a Nugent score > 7 was 21.9% while the specificity was 84.5%. The positive predictive value in our population was 33.7% with a negative predictive value of 75.0%. Conclusion . Though the Nugent score is internally accurate, the prediction of BV using vaginal pH alone has poor sensitivity and specificity., Competing Interests: None of the authors are aware of any financial relationships that would have influenced the conduct, analysis, or outcomes of this study.

One hundred children with primary pyoderma and 50 healthy children were included in the study. The swabs collected from the lesions were cultured on various media. The colonies were identified by conventional methods. Commonest isolate was Staph. aureus (45%), followed by Strep. pyogens (35%), E. coli (5%), Citrobacter (1%) and Staph. and Strept. together (14%). Staphylococci showed highest resistance to ampicillin (85%), followed by penicillin (78%), tetracycline (40%) and ciprofloxacin (15%). Streptococci and other Gram-negative isolates were sensitive to most of the drugs. Most strains of staphylococci were nontypable (42.2%) suggesting the possible emergence of new strains. Among typable ones, phage group-1 was commonest.