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Your search keyword '"Nagaya, Hidekazu"' showing total 21 results
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21 results on '"Nagaya, Hidekazu"'

11. Biological activities of single-chain recombinant goldfish follicle-stimulating hormone and luteinizing hormone

Author

Hayakawa, Youichi, Morita, Tetsuro, Kitamura, Wataru, Kanda, Shinji, Banba, Akira, Nagaya, Hidekazu, Sohn, Young Chang, Yoshizaki, Goro, and Kobayashi, Makito

Subjects
endocrine system, fungi, FSH, Goldfish, GTH, LH, Recombinant, Single-chain, sense organs
Abstract

We produced single-chain recombinant gonadotropins (rGTHs), follicle-stimulating hormone (rFSH) and luteinizing hormone (rLH) of goldfish Carassius auratus, in which the FSHβ or LHβ subunit was fused to the α subunit, by baculovirus in silkworm larvae Bombyx mori. Biological activities of rGTHs were confirmed in vivo by using male goldfish, female bitterling Rhodeus ocellatus ocellatus, and male Japanese eel Anguilla japonica. Administration of rFSH or rLH induced milt production in male goldfish. rLH induced ovulation in bitterling but rFSH showed lower potency. Both rLH and rFSH induced spermatogenesis in male eels after 6 administrations of rGTHs at seven days interval. Spermatozoa were observed in rLH-administered fish. It is suggested that the single-chain goldfish GTHs could be used for the induction of gonadal development in aquaculture fishes.

Published
2008
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19. Cytotoxic triterpenes from Cleome africana

Author

Nagaya, Hidekazu, primary, Tobita, Yoneko, additional, Nagae, Toshiki, additional, Itokawa, Hideji, additional, Takeya, Koichi, additional, Halim, Ahmed F., additional, and Abdel-Halim, Osama B., additional

Published
1997
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20. Recombinant protein production by a Kaiko-baculovirus system.

Author

Nagaya H

Subjects
Animals, High-Throughput Screening Assays methods, Molecular Biology methods, Plasmids genetics, Recombinant Proteins isolation & purification, Transfection, Bombyx genetics, Nucleopolyhedroviruses genetics, Recombinant Proteins biosynthesis, Recombinant Proteins genetics
Abstract

Nucleopolyhedrovirus, a baculovirus, generates many intranuclear polyhedra in lepidopterous insects. The replacement of the polyhedra gene with a target gene, under a potent polyhedrin promoter, is widely used to express recombinant proteins. In this chapter, we describe the application of a highly efficient and reproducible baculovirus expression system with high throughput using Kaiko (silkworm).

Published
2009
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21. N-linked glycan structures of mouse interferon-beta produced by Bombyx mori larvae.

Author

Misaki R, Nagaya H, Fujiyama K, Yanagihara I, Honda T, and Seki T

Subjects
Animals, Bombyx, Interferon-beta genetics, Larva, Mice, Molecular Weight, Protein Conformation, Protein Engineering methods, Protein Isoforms chemistry, Protein Isoforms metabolism, Protein Structure, Tertiary, Recombinant Proteins, Species Specificity, Structure-Activity Relationship, Interferon-beta chemistry, Interferon-beta metabolism, Polysaccharides chemistry, Polysaccharides metabolism
Abstract

The full-length mouse interferon-beta (mIFN-beta) cDNA, including the secretion signal peptide coding region under control of the polyhedrin promoter, was introduced into Bombyx mori nucleopolyhedrovirus (BmNPV). Recombinant mIFN-beta (rmIFN-beta) was accumulated in the haemolymph of infected silkworm larvae. Western blot analysis showed isoforms of rmIFN-beta, suggesting that rmIFN-beta is glycosylated. The glycan structures of purified rmIFN-beta were determined. The N-glycans were liberated by hydrazinolysis and the resulting oligosaccharides were labeled with 2-aminopyridine. The pyridylaminated (PA) glycans were purified by gel filtration, reversed-phase HPLC, and size-fractionation HPLC. The structures of the PA-sugar chains were identified by a combination of two-dimensional PA-sugar chain mapping, MS analysis, and exoglycosidase digestions.

Published
2003
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