Search

Currently, special attention is paid to the study of the effectiveness of the immobilization method—microencapsulation. The aim of the research is to obtain a complex enzyme preparation from pepsin and papain by sequential microencapsulation of enzymes in a pseudo-boiling layer and to evaluate its tenderizing effect on pork. The objects of research were enzymes: pepsin and papain, which were microencapsulated in a protective coating of maltodextrin. It was found that the biocatalytic activity of the complex enzyme preparation is higher than that of pure enzymes. Microencapsulation allows maintaining the high proteolytic activity of enzymes for a long storage period. It has been shown that the thickness of the protective layer during microencapsulation of pepsin and papain in the pseudo-boiling layer of maltodextrin should be in the range of 4–6 microns. During the research, the physicochemical properties of pork were studied depending on the duration of fermentation. It was found that the maximum activity of immobilized enzymes is shifted to the alkaline side. Pork salting with the use of a microencapsulated enzyme preparation in the brine increases the water-binding capacity of proteins to a greater extent in comparison with brine with pure enzymes. The presented data show the high efficiency of sequential microencapsulation of the enzyme pepsin and then papain into a protective layer of maltodextrin in order to preserve their activity during storage.

Currently, various adverse factors can lead to disruption of the immune system. In order to restore the functional state of immunity, it is necessary to use immunomodulators. One such immunomodulators is an enzymatic hydrolyzate of the lymphoid tissue of broiler chickens. The aim of this work was to study the effect of an enzymatic hydrolyzate of the lymphoid tissue of broiler chickens on the morphofunctional state of normal and tumor cells. The object of the research was stem cell culture, human dermal fibroblasts and tumor cell cultures: human cervical carcinoma cells HeLa and adenocarcinoma MCF-7 human breast. The cells were incubated in 96-hole tripod in CO2 incubator at 37°C. These cultures were supplemented with dietary supplements in concentrations of 0.05 %; 0.1 %; 0.5 %; 1 %; 5 %; 10 %; 20 %; 25 %; 40 % and 50 %. After 48 hours, the viability of the cells was examined using MTT-analysis. The experiment revealed cytotoxic effects on mesenchymal stem cells and on MCF-7 breast adenocarcinoma cells. An enzymatic hydrolyzate of the lymphoid tissue of broiler chickens did not have a pronounced effect on viability and did not show a cytotoxic effect in relation to human dermal fibroblasts. In the case of HeLa cells, dietary supplements stimulate their proliferative activity. The response from tumor and stem cells requires further study to identify possible action mechanisms of an enzymatic hydrolyzate of the lymphoid tissue of broiler chickens.