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357 results on '"Nachum-Biala, Yaarit"'

4. Genomic Characterization of Three Novel Bartonella Strains in a Rodent and Two Bat Species from Mexico

Author

Gonçalves-Oliveira, Jonathan, Gutierrez, Ricardo, Schlesener, Cory Lee, Jaffe, David A, Aguilar-Setién, Alvaro, Boulouis, Henri-Jean, Nachum-Biala, Yaarit, Huang, Bihua C, Weimer, Bart C, Chomel, Bruno B, and Harrus, Shimon

Subjects
Microbiology, Biological Sciences, Evolutionary Biology, Genetics, Human Genome, Biotechnology, Bartonella, genomes, rodents, bats, Mexico, Medical microbiology
Abstract

Rodents and bats are the most diverse mammal group that host Bartonella species. In the Americas, they were described as harboring Bartonella species; however, they were mostly characterized to the genotypic level. We describe here Bartonella isolates obtained from blood samples of one rodent (Peromyscus yucatanicus from San José Pibtuch, Yucatan) and two bat species (Desmodus rotundus from Progreso, and Pteronotus parnellii from Chamela-Cuitzmala) from Mexico. We sequenced and described the genomic features of three Bartonella strains and performed phylogenomic and pangenome analyses to decipher their phylogenetic relationships. The mouse-associated genome was closely related to Bartonella vinsonii. The two bat-associated genomes clustered into a single distinct clade in between lineages 3 and 4, suggesting to be an ancestor of the rodent-associated Bartonella clade (lineage 4). These three genomes showed

Published
2023

24. Phylogenetic and network analysis of Pediculus humanus in Nigeria reveal the presences of clade E body lice and novel haplotypes

Author

Kamani, Joshua [0000-0003-1133-2281], Sacristán, Irene [0000-0002-4169-4884], Nachum-Biala, Yaarit [0000-0001-7336-4698], Harrus, Shimon [0000-0003-0542-207X], Kamani, Joshua, Sacristán, Irene, Nachum-Biala, Yaarit, Shand, Mike, Harrus, Shimon, Kamani, Joshua [0000-0003-1133-2281], Sacristán, Irene [0000-0002-4169-4884], Nachum-Biala, Yaarit [0000-0001-7336-4698], Harrus, Shimon [0000-0003-0542-207X], Kamani, Joshua, Sacristán, Irene, Nachum-Biala, Yaarit, Shand, Mike, and Harrus, Shimon

Abstract

An investigation was conducted for the first time to determine the prevalence and genetic diversity of human lice, for the first time in Nigeria, using conventional PCR and sequencing methods. Three mitochondrial genes, cytochrome oxidase subunit 1 (cox1), cytochrome b (cytb), and 12S rRNA of Nigerian human lice, were amplified, sequenced, and analyzed. Overall, high prevalence (72.5%; 103/142) of lice infestation was recorded among the examined volunteers. Head lice infestation was more common 63 (61.2%) than body lice infestation 34 (33.0%). Co-infestation with both head and body lice was recorded in six humans (5.8%). The Nigerian human lice specimens were placed mostly into clade A with few in clade E, including body lice for the first time. Six, three, and eight haplotypes of Nigerian human lice were obtained for the cytb, cox1, and 12S rRNA genes, respectively. Additionally, one (E51), three (A31, A32, and E5), and six (A20, A21, A23, A24, A30, and E1) novel haplotypes were recorded for cox1, cytb, and 12S rRNA, respectively, from the Nigerian specimens which were corroborated by the ML phylogenetic trees and MJ network analyses. Genetic diversity indices indicate minimal variation in the parameters analyzed among the clades of the three genes. However, a statistically significant Snn test, negative Tajima's D test for clade A (cox1 and 12S rRNA genes), and negative Fu and Li's D test in clade A for cox1 gene indicate a geographical structure and the signature of population expansion of the Nigerian human lice. The findings from this study provide additional data on the human lice structure in Africa.

Published
2023

25. Isolation and Genetic Characterization of the Human Relapsing Fever Spirochete Borrelia persica from a Dog with Improved Cultivation Techniques.

Author

Shwartz, Dor, Nachum-Biala, Yaarit, Ben-Shitrit, Bar, Straubinger, Reinhard K., and Baneth, Gad

Abstract

Borrelia persica is the causative agent of tick-borne relapsing fever in Israel and is prevalent in the Eastern Mediterranean basin and parts of Asia. Infection with B. persica causes severe illness and potentially life-threatening complications in humans and companion animals. Isolation and in vitro cultivation of B. persica in culture medium is difficult and there are only two previous isolates of this spirochete. Here, we describe the first cultivation of B. persica from an ill dog. Isolation from the dog's blood was performed with the Pettenkofer-LMU Bp medium and spirochetes with vibrant motility and cell density of 2 × 106/ml were observed in culture by dark-field microscopy 3 days after sampling. The isolate was passaged every 3–4 days with cell densities reaching up to 3 × 107/ml achieved over 30 passages. Cryopreservation was made at −80°C without any cryoprotectant additive, and successful growth from thawed culture samples was observed up to 3 months after freezing with repeated freeze and thaw cycles. Generation time during the exponential growth phase was 14.6 hr. Genetic characterization by polymerase chain reaction amplification and DNA sequencing of the flagellin, glycerophosphodiester phosphodiesterase, and 16S rRNA genes indicated that the isolate is a B. persica genotype I strain associated with human relapsing fever. The new canine isolate would be helpful for research on the pathogenesis of relapsing fever and the new modifications in cultivation and preservation methods may assist in future studies of relapsing fever by simplifying previously reported in vitro methods. [ABSTRACT FROM AUTHOR]

Published
2024
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34. Molecular detection of Bartonella quintana, Acinetobacter baumannii and Acinetobacter haemolyticus in Pediculus humanus lice in Nigeria, West Africa.

Author

Kamani, Joshua, Nachum‐Biala, Yaarit, Bukar, Laminu, Shand, Mike, and Harrus, Shimon

Subjects
*ACINETOBACTER baumannii, *LICE, *BARTONELLA, *CYTOCHROME b, *HUMAN DNA, *MEDICAL care
Abstract

The human lice Pediculus humanus is distributed worldwide but, it thrives and flourishes under conflict situations where people are forced to live in crowded unhygienic conditions. Molecular methods were used to identify and screen human lice for the DNA of pathogens of public health importance in an area that has been under insurgency related to religious and political conflicts with tens of thousands of internally displaced people (IDP). DNA of Bartonella quintana, Acinetobacter baumannii and Acinetobacter haemolyticus was detected in 18.3%, 40.0% and 1.7%, respectively, of human lice collected from children in Maiduguri, Nigeria. More body lice than head lice were positive for pathogen's DNA (64.3% vs. 44.4%; χ2 = 1.3, p = 0.33), but the difference was not significant. Two lice samples were found to harbour mixed DNA of B. quintana and A. baumannii. Phylogenetic analysis of the cytochrome b (cytb) gene sequences of the positive lice specimens placed them into clades A and E. This is the first report on the molecular identification of human lice and the detection of the DNA of pathogens of public health importance in lice in Nigeria, West Africa. The findings of this study will assist policy makers and medical practitioners in formulating a holistic healthcare delivery to IDPs. [ABSTRACT FROM AUTHOR]

Published
2024
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35. Haplotypes of Dirofilaria repens from Poland and selected countries of Central, North-Eastern Europe and the Middle East: An evaluation on the relation between the genetic diversity and the geographic distribution of the fast-spreading parasite

Author

Alsarraf, Mustafa, primary, Baneth, Gad, additional, Bogucka-Kocka, Anna, additional, Ciuca, Lavinia, additional, Dwużnik-Szarek, Dorota, additional, Fuehrer, Hans-Peter, additional, Kloch, Agnieszka, additional, Kołodziej, Przemysław, additional, Levytska, Viktoria, additional, Mierzejewska, Ewa Julia, additional, Mihalca, Andrei D., additional, Ionică, Angela Monica, additional, Mushynskyi, Andrii, additional, Nachum-Biala, Yaarit, additional, Alsarraf, Mohammed, additional, and Bajer, Anna, additional

Published
2023
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40. Evaluation of a treatment protocol in dogs with intraspinal spirocercosis

Author

Asiag, Nimrod, primary, Chai, Orit, additional, Yodovner, Sapir, additional, Ruggeri, Marco, additional, Rapaport, Kira, additional, Baneth, Gad, additional, Nachum-Biala, Yaarit, additional, Konstantin, Lilach, additional, Rojas, Alicia, additional, Brenner, Ori, additional, Bruchim, Yaron, additional, and Shamir, Merav H., additional

Published
2022
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50. Genomic structural plasticity of rodent‐associated Bartonella in nature

Author

de Sousa, Keyla Carstens Marques, primary, Gutiérrez, Ricardo, additional, Yahalomi, Dayana, additional, Shalit, Tali, additional, Markus, Barak, additional, Nachum‐Biala, Yaarit, additional, Hawlena, Hadas, additional, Marcos‐Hadad, Evgeniya, additional, Hazkani‐Covo, Einat, additional, de Rezende Neves, Haroldo Henrique, additional, Covo, Shay, additional, and Harrus, Shimon, additional

Published
2022
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