1. Enhanced homeostatic sleep response and decreased neurodegenerative proteins in cereblon knock-out mice
- Author
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Jun-Hyung Jung, Jinhong Kim, Uroos Akber, Na Young Lee, Jeong-won Baek, Jieun Jung, Mincheol Park, Jiseung Kang, Seungje Jeon, Chul-Seung Park, and Tae Kim
- Subjects
Biology (General) ,QH301-705.5 - Abstract
Abstract Energy homeostasis and sleep have a bidirectional relationship. Cereblon (CRBN) regulates energy levels by ubiquitinating the AMP-activated protein kinase(AMPK), an energy sensor. However, whether CRBN participates in sleep is unclear. Here, we examine sleep–wake patterns in Crbn +/+ and Crbn −/− mice during 24-h baseline, 6-h sleep deprivation (SD), and following 6-h recovery sleep (RS). At baseline, overall sleep patterns are similar between genotypes. However, SD decreases CRBN expression in Crbn +/+ mice and increases phospho-Tau, phospho-α-synuclein, DNAJA1 (DJ2), and DNAJB1 (DJ1) in both genotypes, with Crbn −/− mice showing a lesser extent of increase in p-Tau and p-α-synuclein and a higher level of heat shock protein 70 (HSP70), DJ2, and DJ1. During RS, Crbn −/ − mice show increased slow-wave activity in the low-delta range (0.5–2.5 Hz), suggesting higher homeostatic sleep propensity associated with AMPK hyperactivation. By illuminating the role of CRBN in regulating sleep–wake behaviors through AMPK, we suggest CRBN as a potential therapeutic target for managing sleep disorders and preventing neurodegeneration.
- Published
- 2024
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