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The main objective of this study was to determine effect of different heat pretreatments on chemical and antioxidant properties of Melon Manis Terengganu (MMT) seed oil. The seeds were treated with four different heat pretreatment which were untreated (control), roasting, steaming and microwaving. Then the MMT seed oil was extracted from each treated seed using conventional extraction method. The chemical tests determined on the MMT seed oil were free fatty acid (FFA), iodine value (IV), saponification value and fatty acid composition. The antioxidant properties for MMT seed oil evaluated was total phenolic content (TPC), 1,1- diphenyl-2-picrylhdrazyl (DPPH) scavenging capacity and Ferric reducing antioxidant power (FRAP). It was found that different heat pretreatments of MMT seed significantly (p