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15 results on '"NEOFYTOU, Maria"'

4. Contributors

Author

Allen, Stephanie, primary, Beaudet, Arthur L., additional, Charnock-Jones, D. Stephen, additional, Cheng, S.H., additional, Clarke, Angus, additional, Clarke, Caroline S., additional, Clausen, Frederik B., additional, de Wert, Guido, additional, Deans, Zandra C., additional, Dennis Lo, Y.M., additional, Dondorp, Wybo, additional, Fisher, Jane, additional, Gaccioli, Francesca, additional, Gerrish, Amy, additional, Harasim, T., additional, Haselmann, Verena, additional, Hastings, Ros J., additional, Henneman, Lidewij, additional, Hudson, Emma, additional, Kilby, Mark D., additional, Mackie, Fiona L., additional, Morris, Stephen, additional, Muzzey, Dale, additional, Neofytou, Maria, additional, Oepkes, D., additional, Pandya, Pranav, additional, Pertile, Mark D., additional, Quinlan-Jones, Elizabeth, additional, Sacco, Adalina, additional, Schenk, Peter W., additional, Smith, Gordon C.S., additional, van der Schoot, C. Ellen, additional, van El, Carla, additional, Vermeesch, Joris Robert, additional, Verweij, E.J.T., additional, Vossaert, Liesbeth, additional, Wagner, A., additional, Winkelhorst, Dian, additional, Wolstenholme, Nicola, additional, and Young, Elizabeth, additional

Published
2018
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6. Breast Cancer Detection and Treatment Monitoring Using a Noninvasive Prenatal Testing Platform: Utility in Pregnant and Nonpregnant Populations

Author

Lenaerts, Liesbeth, primary, Che, Huiwen, additional, Brison, Nathalie, additional, Neofytou, Maria, additional, Jatsenko, Tatjana, additional, Lefrère, Hanne, additional, Maggen, Charlotte, additional, Villela, Darine, additional, Verheecke, Magali, additional, Dehaspe, Luc, additional, Croitor, Anca, additional, Hatse, Sigrid, additional, Wildiers, Hans, additional, Neven, Patrick, additional, Vandecaveye, Vincent, additional, Floris, Giuseppe, additional, Vermeesch, Joris Robert, additional, and Amant, Frédéric, additional

Published
2020
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8. MeDIP combined with in-solution targeted enrichment followed by NGS: Inter-individual methylation variability of fetal-specific biomarkers and their implementation in a proof of concept study for NIPT

Author

Keravnou, Anna, primary, Ioannides, Marios, additional, Loizides, Charalambos, additional, Tsangaras, Kyriakos, additional, Achilleos, Achilleas, additional, Mina, Petros, additional, Kypri, Elena, additional, Hadjidaniel, Michael D., additional, Neofytou, Maria, additional, Kyriacou, Skevi, additional, Sismani, Carolina, additional, Koumbaris, George, additional, and Patsalis, Philippos C., additional

Published
2018
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9. Predicting fetoplacental chromosomal mosaicism during non‐invasive prenatal testing

Author

Brison, Nathalie, primary, Neofytou, Maria, additional, Dehaspe, Luc, additional, Bayindir, Baran, additional, Van Den Bogaert, Kris, additional, Dardour, Leila, additional, Peeters, Hilde, additional, Van Esch, Hilde, additional, Van Buggenhout, Griet, additional, Vogels, Annick, additional, de Ravel, Thomy, additional, Legius, Eric, additional, Devriendt, Koen, additional, and Vermeesch, Joris R., additional

Published
2018
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10. Whole-genome fetal and maternal DNA methylation analysis using MeDIP-NGS for the identification of differentially methylated regions

Author

Keravnou, Anna, Ioannides, Marios, Tsangaras, Kyriakos, Loizides, Charalambos, Hadjidaniel, Michael D., Papageorgiou, Elisavet A., Kyriakou, Skevi, Antoniou, Pavlos, Mina, Petros, Achilleos, Achilleas P., Neofytou, Maria C., Kypri, Elena, Sismani, Carolina, Koumbaris, George L., and Patsalis, Philippos C.

Subjects
DNA Methylation, Pregnancy Complications/*diagnosis, Fetal Diseases/*diagnosis, DNA/blood, Fetus/metabolism, High-Throughput Nucleotide Sequencing/*methods, Pregnancy Complications/blood, Epigenesis, Genetic, Pregnancy Trimester, First, Chorionic Villi Sampling, Pregnancy, Genome, Human, Fetal Diseases/genetics, Placenta/metabolism, Humans, Biomarkers/*analysis, Fetal Diseases/blood, Immunoprecipitation, Female, Pregnancy Complications/genetics, DNA/*genetics, Maternal Serum Screening Tests
Abstract

DNA methylation is an epigenetic marker that has been shown to vary significantly across different tissues. Taking advantage of the methylation differences between placenta-derived cell-free DNA and maternal blood, several groups employed different approaches for the discovery of fetal-specific biomarkers. The aim of this study was to analyse whole-genome fetal and maternal methylomes in order to identify and confirm the presence of differentially methylated regions (DMRs). We have initially utilized methylated DNA immunoprecipitation (MeDIP) and next-generation sequencing (NGS) to identify genome-wide DMRs between chorionic villus sampling (CVS) and female non-pregnant plasma (PL) and peripheral blood (WBF) samples. Next, using specific criteria, 331 fetal-specific DMRs were selected and confirmed in eight CVS, eight WBF and eight PL samples by combining MeDIP and in-solution targeted enrichment followed by NGS. Results showed higher enrichment in CVS samples as compared to both WBF and PL samples, confirming the distinct methylation levels between fetal and maternal DNA for the selected DMRs. We have successfully implemented a novel approach for the discovery and confirmation of a significant number of fetal-specific DMRs by combining for the first time MeDIP and in-solution targeted enrichment followed by NGS. The implementation of this double-enrichment approach is highly efficient and enables the detailed analysis of multiple DMRs by targeted NGS. Also, this is, to our knowledge, the first reported application of MeDIP on plasma samples, which leverages the implementation of our enrichment methodology in the detection of fetal abnormalities in maternal plasma. 98 e15 e15 ID: 27834155 Accession Number: 27834155. Language: English. Date Revised: 20170316. Date Created: 20161111. Date Completed: 20170316. Update Code: 20170317. Publication Type: Journal Article. Journal ID: 101550220. Publication Model: Electronic. Cited Medium: Internet. NLM ISO Abbr: Genet Res (Camb). Linking ISSN: 00166723. Subset: IM Date of Electronic Publication: 2016 Nov 11. Original Imprints: Publication: Cambridge New York : Cambridge University Press, c2008

Published
2016

11. Targeted capture enrichment assay for non-invasive prenatal testing of large and small size sub-chromosomal deletions and duplications

Author

Neofytou, Maria C., primary, Tsangaras, Kyriakos, additional, Kypri, Elena, additional, Loizides, Charalambos, additional, Ioannides, Marios, additional, Achilleos, Achilleas, additional, Mina, Petros, additional, Keravnou, Anna, additional, Sismani, Carolina, additional, Koumbaris, George, additional, and Patsalis, Philippos C., additional

Published
2017
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12. Cell-Free DNA Analysis of Targeted Genomic Regions in Maternal Plasma for Non-Invasive Prenatal Testing of Trisomy 21, Trisomy 18, Trisomy 13, and Fetal Sex

Author

Koumbaris, George, primary, Kypri, Elena, primary, Tsangaras, Kyriakos, primary, Achilleos, Achilleas, primary, Mina, Petros, primary, Neofytou, Maria, primary, Velissariou, Voula, primary, Christopoulou, Georgia, primary, Kallikas, Ioannis, primary, González-Liñán, Alicia, primary, Benusiene, Egle, primary, Latos-Bielenska, Anna, primary, Marek, Pietryga, primary, Santana, Alfredo, primary, Nagy, Nikoletta, primary, Széll, Márta, primary, Laudanski, Piotr, primary, Papageorgiou, Elisavet A, primary, Ioannides, Marios, primary, and Patsalis, Philippos C, primary

Published
2016
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13. Whole-genome fetal and maternal DNA methylation analysis using MeDIP-NGS for the identification of differentially methylated regions

Author

KERAVNOU, ANNA, primary, IOANNIDES, MARIOS, additional, TSANGARAS, KYRIAKOS, additional, LOIZIDES, CHARALAMBOS, additional, HADJIDANIEL, MICHAEL D., additional, PAPAGEORGIOU, ELISAVET A., additional, KYRIAKOU, SKEVI, additional, ANTONIOU, PAVLOS, additional, MINA, PETROS, additional, ACHILLEOS, ACHILLEAS, additional, NEOFYTOU, MARIA, additional, KYPRI, ELENA, additional, SISMANI, CAROLINA, additional, KOUMBARIS, GEORGE, additional, and PATSALIS, PHILIPPOS C., additional

Published
2016
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14. Effect of temperature, stocking density, feeding conditions and experimental day on the horizontal and vertical distribution of sea bass fry Dicentrarchus labrax (Linnaeus, 1758).

Author

NEOFYTOU, Maria, SOMARAKIS, Stylianos, PAPADAKIS, Vassilis, DIVANACH, Pascal, STERIOTI, Aspasia, and KENTOURI, Maroudio

Subjects
*SEA basses, *AQUARIUMS, *FISH farming, *EFFECT of temperature on fishes, *FISH feeds, *FISH food
Abstract

The working hypothesis of the present study is to verify whether, the spatial distribution of sea bass fry remains invariable under the pressure of certain rearing conditions, due to its extended plasticity or, certain situations lead to specific distributions. for this reason, we investigated and com pared horizontal and vertical distribution of sea bass reared in aquaria, at three different stocking densities (5 ind. l-1, 10 ind. l-1 and 20 ind. l-1), two different temperatures (16 and 23°C) and two different feeding conditions (feeding fish and starving fish). the analysis of the video records and General Linear Models revealed a strong effect of stocking density, feeding condition, and day of experimentation on fish behavior interpreted by the vertical and horizontal distribution of fish. temperature also affected distribution when it was combined with stocking density and feeding condition. however, this effect seemed to be lessened by the effect of the other variables (i.e. stocking density, feeding condition and day of experimentation). the exposure time (chronic stress) to the stocking density, temperature and feeding condition as well as to their additive cumulative effect (variables interactions) was the cause of the development of stereotypic behaviors that increased in occurrence frequency and over an experimental time lapse and gradually led to the weakening and death of the fry (bad welfare). thus, it is assumed that stocking density, feeding condition, day of experimentation and temperature can have negative effects on sea bass "well being" and in order to maintain its welfare during rearing it is important to take all necessary pre cautions of handling when dealing with these variables in the fish environment. [ABSTRACT FROM AUTHOR]

Published
2013

15. Targeted capture enrichment assay for non-invasive prenatal testing of large and small size sub-chromosomal deletions and duplications

Author

Neofytou, Maria C., Tsangaras, Kyriakos, Kypri, Elena, Loizides, Charalambos, Ioannides, Marios, Achilleos, Achilleas P., Mina, Petros, Keravnou, Anna, Sismani, Carolina, Koumbaris, George L., and Patsalis, Philippos C.

Subjects
0301 basic medicine, Male, Physiology, Maternal Health, Aneuploidy, lcsh:Medicine, Chromosome Disorders, Genome, Database and Informatics Methods, 0302 clinical medicine, Pregnancy, Prenatal Diagnosis, Chromosome Duplication, False positive paradox, Medicine and Health Sciences, Genome Sequencing, lcsh:Science, 030219 obstetrics & reproductive medicine, Multidisciplinary, Obstetrics and Gynecology, Genomics, Hematology, 3. Good health, Body Fluids, Blood, Cell-free fetal DNA, Female, Chromosome Deletion, Anatomy, Sequence Analysis, Research Article, Bioinformatics, Prenatal diagnosis, Computational biology, Biology, Research and Analysis Methods, Sensitivity and Specificity, DNA sequencing, Blood Plasma, 03 medical and health sciences, Diagnostic Medicine, medicine, Genetics, Humans, Abnormalities, Multiple, Molecular Biology Techniques, Sequencing Techniques, Management of High-Risk Pregnancies, Molecular Biology, Sequence Assembly Tools, lcsh:R, Reproducibility of Results, Biology and Life Sciences, Computational Biology, medicine.disease, Genome Analysis, 030104 developmental biology, Women's Health, lcsh:Q, Trisomy, Departures from Diploidy, Sequence Alignment
Abstract

Noninvasive prenatal testing (NIPT) using whole genome and targeted sequencing has become increasingly accepted for clinical detection of Trisomy 21 and sex chromosome aneuploidies. Few studies have shown that sub-chromosomal deletions or duplications associated with genetic syndromes can also be detected in the fetus noninvasively. There are still limitations on these methodologies such as the detection of variants of unknown clinical significance, high number of false positives, and difficulties to detect small aberrations. We utilized a recently developed targeted sequencing approach for the development of a NIPT assay, for large and small size deletions/duplications, which overcomes these existing limitations. Artificial pregnancies with microdeletion/microduplication syndromes were created by spiking DNA from affected samples into cell free DNA (cfDNA) from non-pregnant samples. Unaffected spiked samples and normal pregnancies were used as controls. Target Capture Sequences (TACS) for seven syndromes were designed and utilized for targeted capture enrichment followed by sequencing. Data was analyzed using a statistical pipeline to identify deletions or duplications on targeted regions. Following the assay development a proof of concept study using 33 normal pregnancies, 21 artificial affected and 17 artificial unaffected pregnancies was carried out to test the sensitivity and specificity of the assay. All 21 abnormal spiked-in samples were correctly classified as subchromosomal aneuploidies while the 33 normal pregnancies or 17 normal spiked-in samples resulted in a false positive result. We have developed an NIPT assay for the detection of sub-chromosomal deletions and duplications using the targeted capture enrichment technology. This assay demonstrates high accuracy, high read depth of the genomic region of interest, and can identify deletions/duplications as small as 0.5 Mb. NIPT of fetal microdeletion/microduplication syndromes can be of enormous benefit in the management of pregnancies at risk both for prospective parents and health care providers. 12 e0171319 e0171319 ID: 28158220 Accession Number: 28158220. Language: English. Date Revised: 20170224. Date Created: 20170203. Update Code: 20170225. Publication Type: Journal Article. Journal ID: 101285081. Publication Model: Electronic-eCollection eCollection. Cited Medium: Internet. NLM ISO Abbr: PLoS ONE. Comment: Cites: PLoS One. 2010 Nov 16 5(11):e14004. (PMID: 21103372). Cites: PLoS One. 2014 Apr 21 9(4):e95633. (PMID: 24752422). Cites: Prenat Diagn. 2010 Dec 30(12-13):1131-7. (PMID: 20925131). Cites: Clin Chem. 2015 Apr 61(4):608-16. (PMID: 25710461). Cites: Obstet Gynecol. 2015 Sep 126(3):691-2. (PMID: 26287784). Cites: Genet Med. 2006 Nov 8(11):719-27. (PMID: 17108764). Cites: Cold Spring Harb Protoc. 2010 Jun 2010(6):pdb.prot5448. (PMID: 20516186). Cites: Clin Chem. 2012 Jul 58(7):1148-51. 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