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Two new species of endemic and relict cave-dwellers of genus Neobisium Chamberlin, have been described from Turkey and Macedonia (FYROM), respectively. From its phenetically close congener, N. granulatum Beier, N. yozgati n. sp. (from Yozgat, Turkey) is distinguished in many important respects. In addition, N. anaisae n. sp. (from a cave in Krapa, FYROM) differs from its closest forms (N. korabense Ćurčić, N. ohridanum Hadži, and N. vladimirpantici Ćurčić) by many morphological characters and form of different body parts. Both species are illustrated, diagnosed, and thoroughly described. They both represent endemic and relict forms.