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GroundBIRD is a ground-based experiment for the precise observation of the polarization of the cosmic microwave background (CMB). To achieve high sensitivity at large angular scale, we adopt three features in this experiment: fast rotation scanning, microwave kinetic inductance detector (MKID) and cold optics. The rotation scanning strategy has the advantage to suppress $1/f$ noise. It also provides a large sky coverage of 40\%, which corresponds to the large angular scales of $l \sim 6$. This allows us to constrain the tensor-to-scalar ratio by using low $l$ B-mode spectrum. The focal plane consists of 7 MKID arrays for two target frequencies, 145 GHz and 220 GHz band. There are 161 pixels in total, of which 138 are for 144 GHz and 23 are for 220 GHz. This array is currently under development and the prototype will soon be evaluated in telescope. The GroundBIRD telescope will observe the CMB at the Teide observatory. The telescope was moved from Japan to Tenerife and is now under test. We present the status and plan of the GroundBIRD experiment., 7 pages, 5 figures, LTD18 proceeding, Published in JLTP

Background Endosalpingiosis in the lymph nodes of the mesocolon is very rare. We reported a case with appendiceal endometriosis who had endosalpingiosis in the lymph nodes of the mesocolon that was found during laparoscopic ileocecal resection. Case presentation The patient was a 44-year-old woman who had visited a physician for fever, bloody stool, and abdominal pain 1 year earlier. She was diagnosed with ulcerative colitis on colonoscopy, and symptoms improved with oral treatment. A colonoscopy performed 2 months after diagnosis detected a hard, 20-mm submucosal tumor (SMT) in the cecum. On abdominal contrast CT, an intensely stained mass, including a low-density region, was observed in the cecum. A boring biopsy was performed after mucosal resection of the cecal SMT at our hospital, but diagnosis could not be made. Since the possibility of a malignant lesion could not be ruled out, laparoscopic ileocecal resection was performed. In the resected specimen, a 29 × 27 × 21-mm mass was present in the appendicular root. On histopathological examination, appendiceal endometriosis and endosalpingiosis in the lymph nodes around the ileocolic artery were observed. The postoperative course was favorable, and the patient was discharged 7 days after surgery. Conclusion Differentiation of endosalpingiosis in lymph nodes in the mesocolon from lymph node metastasis of adenocarcinoma is important in patients with an abdominal mass.

GroundBIRD is a millimeter-wave telescope to observe the polarization patterns of the cosmic microwave background (CMB). The target science topics are primordial gravitational waves from cosmic inflation and reionization optical depth. Therefore, this telescope is designed to achieve the highest sensitivity at large angular scales, e = 6 - 300. For wide sky observations (~40% full-sky), scanning at a high rotation speed (120°/s) is important to remove atmospheric fluctuations. Microwave kinetic inductance detector (MKID) is utilized with the fast GroundBIRD rotation since its good time response. We have started the commissioning run at the Teide Observatory in the Canary Islands. We report the performance of the telescope, receiver, and data acquisition system, including cooling achievements, observations of astronomical objects, and observations taken during several days ahead of our main survey observations.

Made available in DSpace on 2021-06-25T10:37:29Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-15 United Nations Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) New sanitation systems have been developed to treat, recover energy and nutrients, and permit reuse processes at the source of generation, minimizing water use and flow segregation. Thus, this study was carried out with the objective of evaluating the potential of hybrid constructed wetlands in the removal of organic matter, nutrients, pathogenic microorganisms, and 12 antibiotics from blackwater previously treated by an upflow anaerobic sludge blanket reactor. A hybrid system of constructed wetlands was used, comprised of a horizontal subsurface flow constructed wetland with a total volume of 0.60 m3 followed by a vertical subsurface flow constructed wetland with a total volume of 0.20 m3. Three different hydraulic retention times were comparatively tested (1.0, 2.0, and 3.0 days for the horizontal subsurface flow constructed wetland, and 1.1, 0.9, and 0.4 days for the vertical subsurface flow constructed wetland) in four distinct stages. The plant species used was Canna x generalis. The results from this study demonstrate the potential of constructed wetlands as a suitable technology for post-treatment of segregated domestic wastewater (anaerobically-digested blackwater). Efficient reduction of COD, BOD5, total nitrogen, and total phosphorus (74, 93, 50, and 61%, respectively) was achieved, with a hydraulic retention time of 3.0 and 1.1 days for horizontal and vertical subsurface flow constructed wetland, respectively (stage IV). The presence of ciprofloxacin was confirmed by chromatographic and mass spectrometric analysis in an average concentration of 442.6 ng.L−1 at the inflow of the horizontal subsurface flow constructed wetland, but was not observed at the outflow. University of São Paulo (USP) São Carlos School of Engineering Department of Hydraulics and Sanitation São Paulo State University (UNESP) School of Engineering Bauru Department of Civil and Environmental Engineering Biological Processes Laboratory Center for Research Development and Innovation in Environmental Engineering São Carlos School of Engineering (EESC) University of São Paulo (USP) São Carlos Institute of Chemistry University of São Paulo São Paulo State University (UNESP) School of Engineering Bauru Department of Civil and Environmental Engineering CAPES: 001 FAPESP: 2013/50351-4

Microwave kinetic inductance detectors have a variety of potential applications in astronomical observations. We built a data acquisition system for kinetic inductance detectors combining a dedicated analog board and a commercially available digital board to meet the requirements of astronomical measurements, such as observation of the cosmic microwave background. This paper reports the status of the development of the data acquisition system. We have already achieved simultaneous readout through 120 channels using a direct down-conversion method to decode the signal. A variety of software has been developed and tested using the functionalities of the system and actual detectors.

The GroundBIRD telescope aims to detect B-mode polarization of the cosmic microwave background radiation using the kinetic inductance detector array as a polarimeter. For the readout of the signal from detector array, we have developed a frequency division multiplexing readout system based on a digital down converter method. These techniques in general have the leakage problems caused by the crosstalks. The window function was applied in the field programmable gate arrays to mitigate the effect of these problems and tested it in algorithm level.

Made available in DSpace on 2018-12-11T17:23:59Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-12-20 The use of anaerobic biomass attached to a support has been recently presented as a good prospect in the treatment of wastewater containing recalcitrant compounds, such as sulfamethazine (SMZ). SMZ has been found in swine wastewater and sewage treatment plants, which motivates assessing their degradation by new wastewater treatment technologies. Thus, this paper describes the use of a continuous fixed structured bed bioreactor for the purpose of evaluating SMZ removal kinetics present in lab-made wastewater. The analysis of SMZ used online solid-phase extraction coupled to liquid chromatography/tandem mass spectrometry (SPE online-LC-MS/MS). Chemical oxygen demand (COD) was also monitored to evaluate the organic matter removal. The bioreactor was operated under mesophilic conditions (30(Formula presented.)C), with a hydraulic retention time of 24 h. In order to evaluate SMZ removal, four different concentration levels were studied: 200, 400, 600, and 800 ng L−1. COD removal efficiency obtained for filtered effluent kept at 91.01% and there was no interference due to the increase of SMZ concentration. For SMZ, the removal efficiencies were of (Formula presented.) for 200 ng L−1 concentration level; (Formula presented.) for 400 ng L−1; (Formula presented.) for 600 ng L−1, and (Formula presented.) for 800 ng L−1. COD removal kinetics presented a first-order apparent removal rate constant ((Formula presented.)) of (Formula presented.) h−1. SMZ also showed a first-order apparent removal rate constant of (Formula presented.) h−1 for the following concentrations levels: 200, 400, 600, and 800 ng L−1. Biological Processes Laboratory, Center for Research, Development and Innovation in Environmental Engineering, São Carlos School of Engineering (EESC), University of São Paulo (USP), Environmental Engineering – Bloco 4-F, São Carlos, SP, Brazil Institute of Chemistry of São Carlos, University of São Paulo, São Carlos, SP, Brazil Institute of Chemistry, Department of Analytical Chemistry, São Paulo State University (UNESP), Araraquara, SP, Brazil

Made available in DSpace on 2019-10-06T16:04:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-03-05 Sulfamethazine (SMZ) is an antibiotic from sulfonamides class widely used in veterinary medicine and reported in wastewater and sewage. Thus, it is essential to study technologies to reduce SMZ present in the aquatic environment. Anaerobic bioreactors are a low-cost technology applied for wastewater treatment. The objective of this paper is to study kinetics parameters related to SMZ removal using a horizontal flow-anaerobic immobilized biomass reactor (HAIB) and to evaluate its transformation products formed during this treatment. The bioreactor was operated at mesophilic condition with a hydraulic retention time of 12 h. The removal of SMZ was evaluated at three different concentrations: 200 ng L−1 (phase I), 400 ng L−1 (phase II) and 600 ng L−1 (phase III). The apparent first-order removal constant obtained for chemical oxygen demand was 0.885 ± 0.094 h−1 while SMZ showed a removal constant of 0.356 h−1. SMZ was removed with an efficiency of 56.0 ± 13.0 % (phase I); 62.0 ± 12.0 % (phase II) and 62.0 ± 6.00 % (phase III). Seven transformation products were detected and one of these with m/z 233 is reported for the first-time. The HAIB bioreactor has a potential to assist in wastewater treatment to remove contaminants at ng L-1 concentration level. Biological Processes Laboratory Center for Research Development and Innovation in Environmental Engineering São Carlos School of Engineering (EESC) University of São Paulo (USP), Environmental Engineering – Bloco 4-F, Av. João Dagnone, 1100 – Santa Angelina, 13.563-120 Federal University of São Paulo Campus Baixada Santista Marine Department, Rua Doutor Carvalho de Mendonça, 144, Encruzilhada São Carlos Institute of Chemistry University of São Paulo, Postal Code 780 Institute of Chemistry Department of Analytical Chemistry São Paulo State University (UNESP), P.O. Box 355 Institute of Chemistry Department of Analytical Chemistry São Paulo State University (UNESP), P.O. Box 355

著者人数: 152名(所属. 宇宙航空研究開発機構宇宙科学研究所(JAXA)(ISAS): 羽澄, 昌史; 堂谷, 忠靖; 長谷部, 孝; 今田, 大皓; 満田, 和久; 坂井, 真一郎; 関本, 裕太郎; Tomida, T.; 辻本, 匡弘; Yamamoto, R.; 山崎, 典子), Accepted: 2019-01-18, 資料番号: SA1180402000

The kinetic inductance detector (KID) is a cutting-edge superconducting detector. The number of KID developers is growing. Most of them have switched from their previous study to superconducting technologies. Therefore, infrastructures for the fabrication of KIDs and cooling systems for their tests have already been established. However, readout electronics have to be newly prepared. Neither a commercial system nor low-cost standard electronics are available despite various attempts to create a standard one. We suggest the use of RedPitaya as readout electronics for the initial step of KID development, which is low cost ( $$\approx $$ 400 USD) and easy to set up. The RedPitaya consists of an all-programmable FPGA-CPU module and a dual-channel 14 bit DAC (ADC) to generate (measure) fast analog signals with 125 MSpS. Each port can be synchronized in-phase or quadrature-phase, and functions for generating and sampling analog signal are prepared. It is straightforward to construct vector network analyzer-like logic by using a combination of these default functions. Up-conversion and down-conversion of its frequency range are also possible by using commercial equipment, i.e., mixers, couplers, and a local oscillator. We implemented direct down-conversion logic on the RedPitaya, and successfully demonstrated KID signal measurements.

Precise measurements of polarization patterns in cosmic microwave background (CMB) provide deep knowledge about the begin of the Universe. The GroundBIRD experiment aims to measure the CMB polarization by using microwave kinetic inductance detector (MKID) arrays. The MKID is suited to multiplexing. One of our requirements is a MUX factor (the number of readout channels for a single wire pair) of at least 100. If we make frequency combs of the MKIDs with 2-MHz spacing, a bandwidth of 200 MHz satisfies 100 MUX. The analog electronics must consist of an analog-to-digital converter (ADC), digital-to-analog converter (DAC), and local oscillator. We developed our own analog electronics board “RHEA.” Two outputs/inputs of DAC/ADC with a 200-MHz clock provide an effective bandwidth of 200 MHz. The RHEA allows us to measure both the amplitude and phase responses of each MKID simultaneously. These data are continuously sampled at a high rate (e.g., 1 kSPS) and with no dead time. We achieved 12 and 14 bits resolution for ADC and DAC, respectively. This corresponds to achieve that our electronics achieved low noise: 1/1000 compared with the detector noise. We also achieved low power consumption compared with that of other electronics development for other experiments. Another important feature is that the board is completely separated from the digital part. Each user can choose their preferred field-programmable array. With the combination of the Kintex-7 evaluation kit from Xilinx, we demonstrated readout of MKID response.

Cosmic microwave background (CMB) is an important source of information about the origin of our universe. In particular, odd-parity large angular scale patterns in the CMB polarization, the primordial B-modes, are strong evidence for an inflationary universe, related to the accelerating expansion of the metric. We are developing a unique telescope, GroundBIRD, to take CMB polarization measurements. The telescope combines novel techniques: high-speed rotation scanning, cold optics, and microwave kinetic inductance detectors (MKIDs). We evaluated the response of MKIDs on the rotation stage. Method of shielding from the geo-magnetic field is established. We have also developed a receiver cryostat. We are able to maintain a sufficient cold status for observations on the optical configuration. We plan to start commissioning the system by observing CMB in Japan in 2015–2016. We will then deploy GroundBIRD in the Canary Islands for further scientific observations.

We search for hidden-photon cold dark matter (HP-CDM) using a spectroscopic system in a K-band frequency range. Our system comprises a planar metal plate and cryogenic receiver. This is the first time a cryogenic receiver has been used in the search for HP-CDM. Such use helps reduce thermal noise. We recorded data for 9.3 hours using an effective aperture area of 14.8 cm$^2$. No signal was found in the data. We set upper limits for the parameter of mixing between the photon and HP-CDM in the mass range from 115.79 to 115.85 $\mu$eV, $\chi < 1.8$-$4.3 \times 10^{-10}$, at a 95% confidence level. This is the most stringent upper limit obtained to date in the considered mass range., Comment: 14 pages, 7 figures

The cosmic microwave background (CMB) radiation is an afterglow of the Big Bang. It contains the crucial keys to understand the beginning of the universe. In particular, the odd-parity patterns of CMB polarization, B-modes, at more than degree-scale, are the best probe to detect primordial gravitational waves at the cosmic inflation. The GroundBIRD experiment aims to detect this large angular scale patterns from the ground. The experiment employs novel techniques; a high-speed rotational scanning system (20 revolution-per-minutes) with cold optics below 4K, and microwave kinetic inductance detectors (MKIDs) as the focal plane detectors. The fast scanning modulation is a crucial characteristic in our observation strategy to mitigate effects of the atmospheric fluctuation. The telescope rotates and scans the sky along the azimuth at the elevation angle of 60 degrees at Teide observatory in the Canary Islands. It allows us to measure CMB polarization patterns at a wide multipole range, 6 < \ell < 300, i.e. aiming to catch the reionization bump. We have developed a telescope mount with 3-axis rotation mechanism (azimuth, elevation, and boresight). We are evaluating the vibration at the focal plane position with rotating the telescope mount. The focal plane consists of seven hexagonal corrugated horn coupled MKIDs array: six hexagon units are for 145 GHz band (55 pixels/unit), and one unit is for 220 GHz band (112 pixels). Each pixel consists of a corrugated horn, a planner OMT, millimeter wave circuits for transmission of dual-polarization signals with the suppression of crosstalk modes, and two MKIDs for each polarization. Magnetic shields are also mounted so as to suppress the external magnetic fields. Trapped magnetic fields inside of the superconducting materials decrease the performance of the MKID. The geomagnetism is the static and large magnetic fields. The telescope motion makes modulation of the geomagnetism as well as the modulation of CMB signals. Therefore, we need careful evaluation associating with the telescope rotation. By using a small evaluation system with modulated magnetic fields, we understand impacts the magnetic shield as well as responses of the MKID for the modulated magnetic field. We design the shield based on them. In this presentation, we will report an evaluation of detector responses on the high-speed rotating system along the azimuth. We will also show demonstrations of our own readout electronics which is well matching with the rapid scan modulation strategy.

著者人数: 153名（所属. 宇宙航空研究開発機構宇宙科学研究所(JAXA)(ISAS): 堂谷, 忠靖; 羽澄, 昌史; 今田, 大皓; 満田, 和久; 坂井, 真一郎; Tomida, T.; 辻本, 匡弘; Yamamoto, R.; 山崎, 典子）, Accepted: 2018-04-30, 資料番号: SA1180210000

This study presents a column-switching solid-phase extraction online-coupled to a liquid chromatography/tandem mass spectrometry (SPE-LC-MS/MS) method for simultaneous analysis of 12 antibiotics (7 sulfonamides and 5 fluoroquinolones) and caffeine detected in the sewage and effluent of a pilot anaerobic reactor used in sewage treatment. After acidification and filtration, the samples were directly injected into a simple and conventional LC system. Backflush and foreflush modes were compared based on the theoretical plates and peak asymmetry observed. The method was tested in terms of detection (MDL) and quantification limit (MQL), linearity, relative recovery, and precision intra- and inter-day in lab-made sewage samples. The method presented suitable figures of merit in terms of detection, varying from 8.00 × 10(-5) to 6.00 × 10(-2) ng (0.800 up to 600 ng L(-1); caffeine) with direct injection volume of only 100 μL and 13 min of total analysis time (sample preparation and chromatographic run). When the method was applied in the analysis of sewage and effluent of the anaerobic reactor (n = 15), six antibiotics and caffeine were detected in concentrations ranging from 0.018 to 1097 μg L(-1). To guarantee a reliable quantification, standard addition was used to overcome the matrix effect.

著者人数: 152名（所属. 宇宙航空研究開発機構宇宙科学研究所(JAXA)(ISAS): 長谷部, 孝; 堂谷, 忠靖; 羽澄, 昌史; 今田, 大皓; 満田, 和久; 坂井, 真一郎; 関本, 裕太郎; Tomida, T.; 辻本, 匡弘; Yamamoto, R.; 山崎, 典子), Accepted: 2018-04-07, 資料番号: SA1180209000

Summary Esophageal squamous cell cancer (ESCC) is a high-grade carcinoma that is treated with multidisciplinary approaches, including chemoradiotherapy (CRT) followed by surgery. Despite some success with these therapies, overall survival remains poor. In order to investigate a newer CRT regimen, we designed a comparative study to evaluate preoperative CRT using docetaxel (DOC) or 5-Fluorouracil and cisplatin (FU+CDDP [FP] therapy) for treatment of resectable ESCC. In a retrospective review of patients with resectable, locally advanced ESCC, 95 patients received preoperative CRT between 2001 and 2007. CRT was administered using either FP (n = 40) or DOC (n = 55). Pathological response and clinical outcomes were compared between the two groups. Hazard ratios and time-to-event analyses were used to assess outcomes; the ratios were controlled by multivariate logistic regression analysis of potential prognostic factors, and survival was presented with Kaplan–Meier curves. In the FP group, a significant curative effect was observed on the basis of pathological examination of postoperative lesions. However, the DOC group presented a significantly better prognosis on the basis of cumulative survival rates. Logistic regression analysis revealed that the presence of five or more lymph node metastases was an independent predictor of reduced survival. Patients with lymph node metastasis exhibited a better prognosis in the DOC group than those in the FP group. Preoperative CRT for locally advanced esophageal cancer using DOC results in similar or better long-term outcomes compared with FP-based CRT. Therefore, CRT using DOC is a promising therapy option for esophageal cancer.

Polarized patterns in the cosmic microwave background (CMB) radiation contains rich knowledge for early stage of the universe. In particular their odd-parity patterns at large angular scale (> 1°), primordial B-modes, are smoking-gun evidence for the cosmic inflation. The GroundBIRD experiment aims to detect these B-modes with a ground-based apparatus that includes several novel devices: a high-speed rotational scan system, cold optics, and microwave kinetic inductance detectors (MKIDs). We plan to start observations in the Canary Islands in 2017. In this paper, we present the status of the development of our instruments. We established an environment that allows operation of our MKIDs in an optical configuration, in which the MKIDs observe radiations from the outside of the telescope aperture. We have also constructed MKID prototypes, and we are testing them in the optical configuration.

Visualization of pathogenic protein aggregates is crucial to elucidate pathomechanisms and to make an accurate diagnosis in many neurodegenerative conditions. Aggregates of the microtubule-binding protein, tau, are one of the most important pathogenic molecules in neurodegenerative disorders. Progressive supranuclear palsy (PSP) is characterized by the deposition of tau proteins in some specific area such as the basal ganglia and brainstem. We tried to detect tau lesions in the brains of living patients with PSP with a novel positron emission tomography (PET) tracer, [Paraffin-embedded brain sections of the patients with PSP were used for autoradiography with [Autoradiography in the brain sections of patients with PSP demonstrated [We conclude that [

To investigate the effects of pH on the structure and the properties of DNA anisotropic gels and their growth process, we have observed the morphology of DNA anisotropic gel films prepared from DNA solutions with various initial pH, and measured the pH dependences of the shrinking ratio, the birefringence, and the relaxation modulus of the gel as well as the time courses of the gel front and pH-change front lines. The gel films prepared from DNA solutions with high pH have inhomogeneous macroscopic structure, large shrinking ratio, and high optical anisotropy whereas those prepared from DNA solutions with low pH have homogeneous macroscopic structure, small shrinking ratio, and low optical anisotropy. The difference observed at different pH is attributed to the difference in the interaction between DNA molecules and aluminum cations. The time courses of the gel front and pH-change front lines were analyzed with theories based on assumptions for each condition. Both two-stage dynamics observed under high initial pH and one-stage dynamics under low initial pH were explained consistently with the theories.

Our understanding of physics at very early Universe, as early as 10−35 s after the Big Bang, relies on the scenario known as the inflationary cosmology. Inflation predicts a particular polarization pattern in the cosmic microwave background, known as the B-mode yet the strength of such polarization pattern is extremely weak. To search for the B-mode of the polarization in the cosmic microwave background, we are constructing an off-axis rotating telescope to mitigate systematic effects as well as to maximize the sky coverage of the observation. We will discuss the present status of the GroundBIRD telescope.

The antibiotics sulfamethoxazole (SMTX) and ciprofloxacin (CIP) are commonly used in human and veterinary medicine, which explains their occurrence in wastewater. Anaerobic reactors are low-cost, simple and suitable technology to wastewater treatment, but there is a lack of studies related to the removal efficiency of antibiotics. To overcome this knowledge gap, the objective of this study was to evaluate the removal kinetics of SMTX and CIP using a horizontal-flow anaerobic immobilized biomass reactor. Two different concentrations were evaluated, for SMTX 20 and 40 μg L(-1); for CIP 2.0 and 5.0 μg L(-1). The affluent and effluent analysis was carried out in liquid chromatography/tandem mass spectrometry (LC-MS/MS) with the sample preparation procedure using an off-line solid-phase extraction. This method was developed, validated and successfully applied for monitoring the affluent and effluent samples. The removal efficiency found for both antibiotics at the two concentrations studied was 97%. Chemical oxygen demand (COD) exhibited kinetic constants that were different from that observed for the antibiotics, indicating the absence of co-metabolism. Also, though the antibiotic concentration was increased, there was no inhibitory effect in the removal of COD and antibiotics.

A L-ascorbic acid biosensor based on ascorbate oxidase has been developed. The enzyme was extracted from the mesocarp of cucumber (Cucumis sativus) by using 0.05 mol L-1 phosphate buffer, pH 5.8 containing 0.5 mol L-1 NaCl. After the dialysis versus phosphate buffer 0.05 mol L-1 pH 5.8, the enzyme was immobilized onto nylon net through glutaraldehyde covalent bond. The membrane was coupled to an O2 electrode and the yielding reaction monitored by oxygen depletion at -600 mV using flow injection analysis optimized to 0.1 mol L-1 phosphate buffer pH 5.8, as the carrier solution and flow-rate of 0.5 mL min-1. The ascorbic acid calibration curve was linear from 1.2x10-4 to 1.0x10-3 mol L-1. The evaluation of biosensor lifetime leads to 500 injections. Commercial pharmaceutical samples were analyzed with the proposed method and the results were compared with those obtained by high-performance liquid chromatography (HPLC).Desenvolveu-se um biossensor para ácido L-ascórbico empregando ascorbato oxidase. A enzima foi extraída do mesocarpo de pepino com solução tampão fosfato 0,05 mol L-1, pH 5,8 contendo NaCl 0,5 mol L-1. Após diálise versus solução tampão fosfato 0,05 mol L-1, pH 5,8 a enzima foi imobilizada em rede de nylon através de ligação covalente com glutaraldeído. A membrana foi acoplada em eletrodo de O2 e a reação monitorada pelo consumo de oxigênio a -600 mV em análise em fluxo (solução tampão fosfato 0,05 mol L-1, pH 5,8 como carregador e vazão 0,5 mL min-1). A curva analítica apresentou-se linear entre 1,2x10-4 a 1,0x10-3 mol L-1. O tempo de vida do biossensor foi de 500 análises. Amostras de medicamentos foram analisadas com a metodologia proposta e os resultados comparados com os obtidos com HPLC.

The malate dehydrogenase (MDH) and ascorbate oxidase were immobilized independently, onto silanized controlled porous silica and packed in a tygon tube. The reactors were inserted in the flow system, and the malic acid was determined by measurement of NADH produced by enzymatic reaction. The NADH was reoxidized in a wall jet cell that consisted of spectrographic graphite, Ag/AgCl, KCl(sat), and steel needle as work, reference, and counter electrodes, respectively. The current intensities were measured at 390 mV. The malate calibration curve shows a linear range from 5.0 × 10−6 to 1.0 × 10−4 mol L−1, the lifetime was 40 analyses, after that a decrease of 20% on the response is observed. Three different citric juices were analyzed and a good correlation between the proposed method and spectrophotometric commercial kit were obtained.

MUSES-C, a Japanese sample return mission, is targeting a small near Earth asteroid, 1998SF36, which is considered an S-type asteroid and is similar in spectroscopy to LL class ordinary chondrite meteorite ( Binzel et al . 2001 ). Although this mission will bring us detailed photometric data, that is, disk-resolved bidirectional reflectance data of the asteroid, there were few bidirectional reflectance data of ordinary chondrite meteorites. For the purpose of comparison with the data obtained by the in-situ observation, we have performed measurements of bidirectional reflectance of ordinary chondrite samples. Here we summarize the result of our laboratory measurements of the bidirectional reflectance and compare them with the scattering property of 1998SF36. Although the geometric albedo of 1998SF36 is higher than the typical value of S-type asteroids, however, the laboratory data of ordinary chondrite are similar to or brighter than the model disk-resolved reflectance of 1998SF36 derived from disk-integrated ground-based data. We found in our laboratory data that there is a positive correlation between the surface roughness and the strength of the opposition effect. A stronger opposition effect in the reflectance of 1998SF36 than the laboratory data may therefore indicate that the surface of the asteroid has rougher structure than our laboratory samples.

SUMMARY. In 97 patients (60, chemotherapy; 22, chemoradiotherapy; 15, radiotherapy), histopathologic effects were evaluated microscopically, and histologic response rates were compared among three neoadjuvant treatment modalities. Predictive factors for neoadjuvant therapies were analyzed by logistic regression, including the results of p53 immunohistochemical staining. In the chemoradiotherapy group, the pathologic response rate was 86.4%, and was significantly higher than that for chemotherapy (P

The electrogenerated chemiluminescence (ECL) coupled to a flow injection analysis system was used for the determination of cefadroxil. In this study, the tris(2,2′-bipyridine)ruthenium(II) complex, whose luminescence increased with the presence of the cefadroxil was used. A linear equation between the luminescence peak intensity and the cefadroxil concentration, was obtained for deoxygenated solutions over the range of 5×10−8 to 1×10−4 mol l−1. No interference was observed and the luminescence method was successfully applied to the determination of cefadroxil in a commercial product, being the recoveries in the order of 100%.

The control system for stabilizing the lateral motion at landing the aerospace plane (ASP) onto moving ekranoplane is developed. As the disturbances character applied to ASP are being changed at different weather conditions, the system analysis and synthesis are made in a wide class of disturbances. This class may be represented by some numerical characteristics - the upper bounds of derivatives dispersions. The way for estimation of such bounds by means of current observations is proposed. The algorithm for adapting the controller in accordance with the current bounds of dispersions of the input signal. It allows to increase the accuracy and reliability of docking the ASP and ekranoplane.

Objective. Numerous cytokines are expressed in lesions of synovial hyperplasia of patients with rheumatoid arthritis (RA), and their pathophysiological contributions have been the subject of speculation. These genes are regulated by the transcription factor NFkB which in turn is activated by tumour necrosis factor-a (TNF-a) and cytokines. In this study we examined the inhibition of the production of pro-inflammatory cytokines, adhesion molecule and matrix metalloproteinase (MMP) from synovial tissue of patients with RA by the introduction of synthetic double-stranded DNA with high affinity for the NFkB binding site. Method. NFkB decoy oligonucleotides (ODN ) were introduced with the aid of the haemagglutinating virus of Japan (HVJ )-liposome method into synovial tissue or synovial cells derived from patients with RA. The levels of interleukin-1b (IL-1b), IL-6, TNF-a, intercellular adhesion molecule-1 (ICAM-1) and MMP-1 were determined by means of enzyme-linked immunosorbent assay ( ELISA) and Northern blotting analysis. A cell counting kit was used to study the effect of NFkB decoy ODN on synovial cell proliferation. Results. The production of these mediators was significantly inhibited by the introduction of NFkB decoy ODN compared with the effect of scrambled decoy ODN. Transfection of NFkB decoy ODN resulted in a significant inhibition of synovial cell proliferation as compared with that of scrambled decoy ODN. Conclusion. The results demonstrated in this study suggest the potential usefulness of NFkB decoy ODN for gene therapy of inflammatory synovitis of RA. K : Transcription factor decoy, Gene therapy, Rheumatoid arthritis, HVJ-liposome, Synovial cells.

The aim of this work was to develop an intraoperative image-guidance system for breast cancer surgery using three-dimensional (3D) ultrasound imaging. Using a 10-MHz annular array mechanical sector probe, ultrasound images were obtained from nine volunteer patients with breast cancer immediately before removal of the tumor in the operating room. A 3D tumor image was reconstructed using a workstation, then superimposed on the video image of the breast based on geometrical data. These data were obtained simultaneously by an optical 3D position sensor. The 3D images of the tumors were validated by the pathological data obtained after the surgery. In eight cases, the superimposed images were successfully obtained in approximately 15-20 min following scanning of the tumor. Scattered lesions around the main tumor were also visualized in the reconstructed tumor images, but artifacts of the ductal lesion caused by noise could not be eliminated in some cases. This system should be very effective in helping the surgeon to recognize the extent of a tumor within the breast itself and to determine the margin of surgical resection for breast conservation surgery.

By using a retrovirus-derived system we generated derivatives of the human colon adenocarcinoma cell line LS174T (ATCC CL 188) that stably overexpress a full-length cDNA encoding the beta 1 isoform of bovine phosphoinositides-specific phospholipase C (PI-PLC). This was confirmed by the elevated levels of catalytic activity to release phosphoinositides from phosphatidylinositol (PI-PLC) or phosphatidylinositol-bis-phosphate (PIP2-PLC), and the enhanced expressions of messenger RNA and protein. PI-PLC beta 1 overexpresser clones grew to form cell clumps floating in liquid medium, whereas the pMV7-introduced control clones displayed morphologic characteristics that were very similar to those of the parent LS174T cell line. Three individual PI-PLC beta 1 overexpresser cell lines displayed increased doubling time (18.0 h, 21.5 h, and 23.8 h) when compared with 4 individual pMV7-introduced control cell lines (13.1 h, 10.7 h, 12.9 h, and 9.3 h). Anchorage-independent growth ability in soft agar medium was dramatically suppressed by overexpression of PLC beta 1, and the ability of PLC-overproducer clones to form aggregates when cultured in liquid medium was dramatically enhanced when compared with that of pMV7-introduced control clones. Tumorigenicity of PLC beta 1-overproducers was much weaker than that of vector-transduced control clones. The spontaneous release of carcinoembryonic antigen from PLC beta 1-overproducer clones was much higher than that from pMV7 control clones. The ability of PLC beta 1-overproducer clones to form aggregates during suspension culture was much stronger than that of the control clones. These results provide the first evidence that elevated levels of endogenous PI-PLC beta 1 suppress tumor cell growth, but enhance the ability to form cell aggregates and to release carcinoembryonic antigen, an intercellular adhesion molecule.