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Willow trees (Salix spp.) exhibit remarkable genetic and phenotypic diversity, yielding a broad spectrum of bioactive compounds, notably valuable phenolic compounds such as condensed tannins (phenolic polymers), flavonoids, salicylic glucosides, and phenolic compounds. These enhance the economic value of willow crops and make them suitable for circular bioeconomy. Phenolic compounds known for their diverse applications as antioxidants, antimicrobial agents, pharmaceuticals, nutraceuticals and antiseptics and more, find a natural source in willow. This study aimed to elucidate the composition of 12 flavonoids and salicylic acid in different segments of five organic winter dormant willow species (S. daphnoides, S. fragilis, S. dasyclados, S. viminalis, and S. dasyclados x viminalis) using quantitative analysis and providing valuable insights into their high-value phenolic compounds. Separation into buds, wood and bark segments allowed for a precise characterization of the location of certain phenolic compounds and quantification using LC-MS/MS techniques. LC-MS/MS is an analytical technique known for its increased sensitivity and chromatographic precision. Among the findings, catechin emerged as the predominant flavan-3-ol in all Salix species, with the highest concentration in the buds of Salix viminalis (7.26 mg/g DM). Naringenin exhibited species-specific variations, with S. dasyclados and S. viminalis recording the highest levels. Salicylic acid concentrations peaked in S. dasyclados (5.38 mg/g DM) and S. daphnoides (4.43 mg/g DM), particularly within the bark. When evaluating other individual flavonoids and total polyphenol content (TPC), disparities between buds, bark, and wood became evident, with wood consistently displaying the lowest content. Notably, the higher concentration of polyphenolic compounds in willow bark can be attributed to its susceptibility to external threats and its role as a robust defense mechanism against pathogens and herbivores. This st

The primary idea is to develop a new feed additive as an important climate initiative for organic cattle producers to reduce the enteric methane production in dairy cows with 30%. This is expected to be achieved by adding plant extracts from organically grown willow and hemp, plants with a high content of polyphenols such as tannins and flavonoids that inhibit methanogenic microorganisms.

Ruminants contribute to global warming by emitting greenhouse gasses, particularly methane (CH4) which is a product of rumen fermentation. The use of feed additives able to modulate rumen fermentation is a promising strategy to reduce enteric CH4 and ammonia (NH3) emissions. Among the various strategies investigated, plant secondary metabolites (PSMs) have attracted attention due to their apparent potential to reduce enteric CH4 and NH3 emissions, and it would be possible to use such compounds as feed additives in organic production systems. In an in vitro system simulating rumen fermentation, we have tested the impact of different classes of naturally occurring PSMs; catechin and quercetin (flavonoids), salicylic acid (phenolic acid) and tannic acid (hydrolysable tannin). The PSMs were added to two different basal feeds (maize and grass silages) at three inclusion doses 1.5, 3 and 6% of the feed dry matter (DM). CH4 production was significantly lowered upon addition of quercetin to two basal feeds at doses of 3 and 6%, and this without changes in concentrations of total volatile fatty acid (VFA) produced during fermentation. Quercetin, as the only tested additive, reduced CH4 production, and when added to maize silage and grass silage, the reduction increased linearly with increasing dose, ie., by 51 and 43%, respectively, at a dose of 3% of feed DM and by 86 and 58%, respectively, at a dose of 6% of feed DM. Moreover, quercetin significantly reduced NH3 concentration by >12% at doses of 3 and 6% in feed DM irrespective of the basal feed used as compared to when the basal feeds were incubated alone. Although none of the other additives affected CH4 formation, several additives had significant impacts on concentrations of NH3 and VFAs in the incubated fluid after fermentation. This study demonstrated a dose-dependent ability of quercetin to reduce CH4 emission from rumen fermentation, however, the magnitude of the suppression of CH4 depended on the basal feed. Furth

Projektets primære formål er at udvikle et nyt foderadditiv som et vigtigt klimatiltag for økologiske kvægproducenter for at kunne reducere den enteriske metanproduktion hos malkekøer med 30%. Dette forventes opnået gennem tilsætning af planteekstrakter fra økologisk dyrket pil og hamp, som er planter med et højt indhold af kondenserede tanniner, der hæmmer metanogene mikroorganismer. Produkterne screenes in vitro, hvorefter de mest effektive ekstrakter eller kombination af disse indgår i forskningsforløb med emissionsmålinger i klimakamre med levende køer - og senere hos en mindre gruppe dyr under praksisnære forhold.

Background A whole-grain (WG)–rich diet has shown to have potential for both prevention and treatment of the metabolic syndrome (MetS), which is a cluster of risk factors that increase the risk of type 2 diabetes and cardiovascular disease. Different WGs may have different health effects. WG rye, in particular, may improve glucose homeostasis and blood lipids, possibly mediated through fermentable dietary fiber and lignans. Recent studies have also suggested a crucial role of the gut microbiota in response to WG. Objectives The aim was to investigate WG rye, alone and with lignan supplements [secoisolariciresinol diglucoside (SDG)], and WG wheat diets on glucose tolerance [oral-glucose-tolerance test (OGTT)], other cardiometabolic outcomes, enterolignans, and microbiota composition. Moreover, we exploratively evaluated the role of gut microbiota enterotypes in response to intervention diets. Methods Forty men with MetS risk profile were randomly assigned to WG diets in an 8-wk crossover study. The rye diet was supplemented with 280 mg SDG at weeks 4–8. Effects of treatment were evaluated by mixed-effects modeling, and effects on microbiota composition and the role of gut microbiota as a predictor of response to treatment were analyzed by random forest plots. Results The WG rye diet (± SDG supplements) did not affect the OGTT compared with WG wheat. Total and LDL cholesterol were lowered (−0.06 and −0.09 mmol/L, respectively; P < 0.05) after WG rye compared with WG wheat after 4 wk but not after 8 wk. WG rye resulted in higher abundance of Bifidobacterium [fold-change (FC) = 2.58, P < 0.001] compared with baseline and lower abundance of Clostridium genus compared with WG wheat (FC = 0.54, P = 0.02). The explorative analyses suggest that baseline enterotype is associated with total and LDL-cholesterol response to diet. Conclusions WG rye, alone or with SDG supplementation, compared with WG wheat did not affect glucose metabolism but caused transient LDL-choleste

Aims/hypothesis: The phytoestrogen enterolactone is a gut microbiota-derived metabolite of plant lignans with suggested beneficial properties for health. In the current study, we investigated the association between pre-diagnostic plasma enterolactone concentrations and mortality among individuals diagnosed with type 2 diabetes.Methods: In a population of people diagnosed with diabetes, nested within the Danish Diet, Cancer and Health cohort, we conducted a case-cohort study including a random sample of n = 450 cases (deceased) and a randomly selected subcohort of n = 850 (in total n = 617 deaths). Information on diagnosis, vital status and cause of death was obtained from Danish registers. Cox proportional hazard models with special weighting were applied to assess all-cause and cause-specific mortality.Results: The median enterolactone concentration of the current population was low, 10.9 nmol/l (5th percentile to 95th percentile: 1.3–59.6), compared with previously reported concentrations from the Diet, Cancer and Health cohort. Pre-diagnostic enterolactone concentrations were associated with lower all-cause mortality when assessed linearly per doubling in concentration (log 2 ) (HR 0.91 [95% CI 0.85, 0.96]) and according to quartiles (HR 0.63 [95% CI 0.48, 0.84]) for the highest quartile of enterolactone compared with the lowest quartile. For cause-specific mortality, only death from diabetes (registered as underlying cause of death) reached statistical significance. Conclusions/interpretation: Based on this large cohort of people with diabetes with detailed and complete baseline and follow-up information, pre-diagnostic enterolactone concentrations were inversely associated with mortality. To our knowledge, this is the first study on enterolactone and type 2 diabetes mortality. Our findings call for further exploration of enterolact