1. Association of the blood serum cytokines' rate and lymphocytes' apoptosis with polymorphic variants of the BCL-2 (rs17759659), CTLA-4 (rs231775) and APO-1÷FAS (rs2234767) genes in patients with nodular goiters in autoimmune thyroiditis and thyroid adenoma.
- Author
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Sheremet MI, Sydorchuk LP, Shidlovskyi VO, Sydorchuk RI, Shidlovskyi OV, Maksymyuk VV, Bezruk VV, Tkachuk NP, Batig VM, Mytchenok OV, Bezruk TO, and Chympoi KA
- Subjects
- Adult, Aged, Apoptosis physiology, CTLA-4 Antigen blood, Female, Goiter, Nodular blood, Goiter, Nodular pathology, Humans, Lymphocytes metabolism, Middle Aged, Polymorphism, Genetic, Proto-Oncogene Proteins c-bcl-2 blood, Thyroid Neoplasms blood, Thyroid Neoplasms pathology, Thyroiditis, Autoimmune blood, Thyroiditis, Autoimmune pathology, Young Adult, fas Receptor blood, CTLA-4 Antigen genetics, Cytokines blood, Goiter, Nodular genetics, Lymphocytes pathology, Proto-Oncogene Proteins c-bcl-2 genetics, Thyroid Neoplasms genetics, Thyroiditis, Autoimmune genetics, fas Receptor genetics
- Abstract
The paper analyses results of serum cytokines and lymphocyte apoptosis study in patients with nodular goiter against the background of autoimmune thyroiditis (NGAIT) and thyroid adenoma (TA) based on the cell preparedness to apoptosis (content of lymphocytes carrying apoptosis marker - CD95+-receptor), the number of apoptotic lymphocytes (annexin V+-lymphocytes) and the content of proapoptotic tumor necrosis factor-alpha (TNF-α), interleukin (IL)-1β and IL-6, as well as anti-inflammatory IL-4 cytokine in serum, considering the polymorphism of BCL-2 (rs17759659), CTLA-4 (rs231775) and APO-1÷FAS (rs2234767) genes. The results show that under the damaging action of peroxidation products in the thyroid structures, activation of Fas- and caspase-dependent mechanisms of influence on pro- and anti-apoptotic targets, the induced hyperproduction and release of TNF-α from thyroid-stimulated lymphocytes stimulate an additional synthesis of other pro-inflammatory cytokines IL-1β and IL-6, as well as compensatory anti-inflammatory proteins including IL-4. There is a synchronized increase in secretion of the soluble form of TNF-α receptor (sTNFR), which prevents binding the corresponding cytokine to a specific membranous shedding of a number of receptors and separates the apoptotic signals. The above-mentioned changes associate with the polymorphic variants of BCL-2 (rs17759659), CTLA-4 (rs231775) genes and only for some figures, they are almost three times weaker with FAS (rs2234767).
- Published
- 2017