1. Distribution, quantification, and characterization of substance P enteric neurons in the submucosal and myenteric plexuses of the porcine colon.
- Author
-
Mazzoni, Maurizio, Cabanillas, Luis, Costanzini, Anna, Caremoli, Filippo, Million, Mulugeta, Larauche, Muriel, Clavenzani, Paolo, De Giorgio, Roberto, and Sternini, Catia
- Subjects
Cholinergic and nitrergic transmission ,Excitatory motor neurons ,Inhibitory motor neurons ,Interneurons ,Secretomotor neurons ,Humans ,Swine ,Animals ,Myenteric Plexus ,Submucous Plexus ,Substance P ,Neurons ,Colon ,Choline O-Acetyltransferase - Abstract
The pig is an important translational model for studying intestinal physiology and disorders for its many homologies with humans, including the organization of the enteric nervous system (ENS), the major regulator of gastrointestinal functions. This study focused on the quantification and neurochemical characterization of substance P (SP) neurons in the pig ascending (AC) and descending colon (DC) in wholemount preparations of the inner submucosal plexus (ISP), outer submucosal plexus (OSP), and myenteric plexus (MP). We used antibodies for the pan-neuronal marker HuCD, and choline acetyltransferase (ChAT) and neuronal nitric oxide synthase (nNOS), markers for excitatory and inhibitory transmitters, for multiple labeling immunofluorescence and high-resolution confocal microscopy. The highest density of SP immunoreactive (IR) neurons was in the ISP (222/mm2 in the AC, 166/mm2 in the DC), where they make up about a third of HuCD-IR neurons, compared to the OSP and MP (19-22% and 13-17%, respectively, P
- Published
- 2024