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Additional file 1: Figure S1. SEM imaging of ‘Hippo stomach fluke type 1’. Figure S2. The morphometric approach to identify the hippo liver flukes. Figure S3. F. nyanzae metacercariae isolated from P. columella. Figure S4. Primers used to amplify the partial rDNA region of F. nyanzae. Figure S5. COI-based Phylogeny of the Radix genus. Figure S6. COI-based Phylogenies of the genera Bulinus and Biomphalaria. Table S1. Corrected COI genetic distances for the subfamily Fasciolinae. Table S2. Corrected rDNA genetic distances for the subfamily Fasciolinae. Table S3. Corrected COI genetic distances for the genus Radix. Table S4. Corrected COI genetic distances for the genus Biomphalaria. Table S5. Corrected COI genetic distances for the genus Bulinus. Table S6. Accession numbers generated in this study, Carolus et al. [5] and Muzarabani et al. (preprint, [33]).