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Nowadays there is a water crisis, farmers usually use wastewater and well water for agricultural cultivation. A pot experiment was used to investigate the influence of Duhok municipal wastewater for irrigation of broccoli plants of the genus (Brassica oleracea var. Italica (Broccoli) as compared to fresh water on broccoli parts and morphology of the plant. Municipal wastewater can be a rich source of necessary nutrients for plant growth when used as irrigation water. In this investigation, the municipal wastewater was preserved in plastic bottles taken from sewage sludge called (Hishkero) river, while the utilized soil got around the Misuriké district under a loamy soil texture class. The result showed that the broccoli plant was significantly affected by wastewater compared to freshwater in increasing potassium, lead, and copper (1.92 ± 0.05 % ), (1.70 ± 0.05 %), (0.66 ± 0.02 mg/kg-1), (0.56 ± 0.02 mg/kg-1), (0.23 ± 0.03 mg/kg-1), (0.05 ± 0.03 mg/kg-1) respectively. While the broccoli plant for macro-nutrient accumulation (NPK) was significantly accumulated in shoot and head more than roots (1.45 ± 0.04 %), (1.41 ± 0.04 %), (0.24 ± 0.03 %), (0.33 ± 0.03 %), (2.17 ± 0.07 %), (2.11 ± 0.07 %) respectively. In conclusion, the nutrient content in broccoli was improved by wastewater as it has high nutrient contents with permissible levels of hazardous heavy metals of as lead and cadmium especially in the eaten part.

Nowadays, heavy metals are regarded as the most significant contaminants due to industrial activities and have an impact on their presence in milk. The presence of heavy metals in milk could have a serious negative impact on public health. The current study aims to determine the amount of various heavy metals (Pb, Cu, Co, Cd, Cr, Ni, Fe, and Zn) in raw Awassi ewe’s milk collected from Kwashe industrial area, Duhok province, Kurdistan Region, Iraq. The sheep were grazed in Sulaivany plain contaminated by drains of industrial effluents of several crude oil refineries and other 200 different factories. Atomic absorption spectrophotometer was utilized to analyze the milk specimens after wet digestion. Results displayed that the heavy metals concentration was in the range of 3.64-4.27 mg/L for Pb, 0.59-1.13 mg/L for Cu, 0.01-0.09 mg/L for Co, 0.12-1.46 mg/L for Cd, 0.24-0.29mg/L for Cr, 0.89-0.99 mg/L for Ni, 0.89-0.94 mg/L for Fe and 3.99-6.13 mg/L for Zn. Statistical analyses showed that excusing Cr, and all other studied heavy metals concentrations were higher than the human-health safety recommended. Furthermore, among the heavy metals in the current study, the Zn was the highest mean value recorded (4.99 mg/L) and it exceeded the limited value (3.8 mg/L) by WHO/FAO, (1999). The permissible level of pb in milk is 0.05 mg/L, and the mean concentration of pb in the milk sample (3.99 mg/L) was significantly higher than the permissible value. Moreover, the mean value of Cd (0.75 mg/L), Cu (0.79 mg/L), Co (0.038 mg/L), Ni (0.948 mg/L), and Fe (0.91 mg/L) were above the limited value (0.01 mg/L) for Cd, 0.03 mg/l for Cu, (0.001-0.008) for Co, (0.1mg/L) for Ni and 0.1 for Fe, respectively. While the Cr concentration (0.27 mg/L) was lower than the recommended level (0.3 mg/L). Therefore, it was observed that the amount of heavy metals in the sheep milk utilized in this investigation possesses a health risk. Hence, it is always needful to persuade the pollutants in milk in the current area.

The main objective of the present study is to spatially evaluate the water contamination of Khabur River, before it arrives in Zakho City, inside the city, and after it leaves the city of Zakho by heavy metals. Also, the seasonal effects of Zakho municipal wastewater discharged in the Khabur River were detected in this study. The results showed that some heavy metals, such as Ni, were not detected in all studied samples in this study due to their low concentration levels. It is indicated that heavy metals are statistically affected by spatial location and high amounts were detected after leaving, compared to before entering the city which indicates that municipal wastewater is the main source of metal pollution. However, all water tests met the WHO's authorized limits. The average detected concentrations of copper, iron, manganese, and lead ranges from 0.003 to 0.025 mg/L, 0.000 to 0.054 mg/L, and 0.057 to 0.112 mg/L, and 0.014 to 0.135 mg/L respectively, while the highest concentrations of copper and iron were recorded significantly in Bedare (0.025 mg/L, 0.054 mg/L) respectively. The highest concentration of Lead (Pb) was detected in Chamtre and Tawke 0.117 mg/L and 0.135 mg/L respectively and this increase may be due to the presence of oil in the discharged industrial wastewater, in this village. Regarding season’s effects, higher mean concentrations for Mn 0.13423 mg/L, Fe, and 0.04208 mg/L were recorded in the autumn season. However, copper and lead had higher mean concertation 0.02389 mg/L, and 0.097 mg/L respectively, during the winter season, while the lowest mean concentration of copper was recorded in autumn, and lead in the summer season had a minimum concentration. There was a significant difference (P

The high concentration of nitrogen (N) compounds in wastewater was found to be creating a reason of eutrophication, effect the biodiversity of aquatic ecosystems and human health. The aim of this study is to reduce harmful N forms from wastewater by biological nitrogen transforming (BNT) bacteria and to investigate the fluctuation of ammonium oxidizing bacteria (AOB), nitrite oxidizing bacteria (NOB), and pathogenic bacteria during this process, so compost representative. A sample of municipal wastewater was taken, and then subjected to the treatment process of BNT, which was subdivided into three stages of ammonification, nitrification, and denitrification. The result revealed that nitrifying bacteria were flocculated during biological nitrogen transformation, like AOB and NOB isolated until obtaining colonies during the nitrification process, by liquid mineral solution and Agar-Agar then calculated. The AOB count was 468 CFU/ml and NOB was 2130 CFU/ml in the sample. The existence of coliform bacteria, especially E. coli raises the possibility of water contamination by pathogens bacteria that cause many serious illnesses in human. Therefore, Mac-Conkey agar was used for the isolation of total coliform bacteria through the BNT process because it is selective and also differentiating agar that specialized in the growth of gram- negative bacteria. Total coliform bacteria decreased gradually, before ammonification (556 CFU/ml), after ammonification (226 CFU/ml), after nitrification (154 CFU/ml) and after denitrification (45 CFU/ml) that have been determined and indicated that further chemical disinfection like chlorination or ozonation were required for complete sterilization of pathogenic bacteria. The study concluded that all harmful forms of N and total coliform bacteria that cause human and ecosystem deleterious were reduced by BNT.