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Endophytic bacteria, which are the subject of this study, serve as natural antifungal agents in the struggle against fungal infections, offering an eco-friendly alternative to chemical fungicides. So, it was aimed to determine the antifungal capacities of endophytic bacteria from Aronia ×prunifolia roots in the study. 25 endophytic bacteria were isolated, and their ability to act as biocontrol agents was evaluated by measuring fungal growth inhibition and chemical properties. Later, bacteria that showed a positive effect were identified through 16S gene sequencing. The results showed that the LB2 bacteria had the greatest ability to inhibit the selected fungi and the biochemical tests showed that the bacteria were Gramnegative, did not form spores, their colonies were well defined, and they could break down starch and gelatin, which was later diagnosed as Pseudomonas brassicacearum according to phylogenetic relationships. This study is the first report on which P. brassicacearum was isolated from A. ×prunifolia roots for the first time. These findings contribute to our understanding of the potential of endophytic bacteria, particularly P. brassicacearum, as natural antifungal agents in plant and human protection, offering a promising and sustainable approach to combat fungal infections while reducing the use of chemical fungicides. [ABSTRACT FROM AUTHOR]

Endophytes play a pivotal role in sustainable agriculture due to their capacity to generate numerous agriculturally significant metabolites. This study focuses on isolating a salt-tolerant, fluorescent green pigment-producing endophytic bacterium from the leaf samples of Aronia prunifolia cultivated on 2.5% Sodium chloride (NaCl) supplemented nutrient agar. After thoroughly examining the roots' isolated strain, EB-3, it has been identified as Pseudomonas putida. This identification was based on a detailed analysis of its morphological and biochemical characteristics, coupled with the scrutiny of its 16S rDNA sequence. Extensive biochemical and functional studies have revealed the diverse capabilities of P. putida. This bacterium excels in producing hydrogen cyanide (HCN), siderophore, indole acetic acid (IAA), and demonstrates proficiency in phosphate solubilization. These findings emphasize the potential of P. putida as a valuable bacterial inoculant for sustainable agriculture, especially in challenging environments. The versatility of this strain in producing beneficial metabolites underscores its crucial role in promoting agricultural resilience and productivity. Consequently, P. putida emerges as a promising ally in advancing sustainable agricultural practices, particularly in stressful conditions. [ABSTRACT FROM AUTHOR]