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5. Poster display I clinical general

Author

Gurgenyan, S. V. Svetlana, Vatinyan, S. K. H., Nicogosyan, K. G., Edilyan, L. B., Chobanyan, B. G., Graf, S., Beheshti, M., Kienast, O., Oezer, S., Angelberger, P., Dudczak, R., Li, S. Shuren, Azevedo, J. C. Jader, Félix, R. C., Corrêa, P. L., Barbirato, G. B., Mesquita, E. T., Volschan, A., Dohmann, H. F., Mesquita, C. T., Peix, A. Amalia, Hidalgo, J., Dorticos, E., Llerena, L., Paredes, A., Macias, C., Valle, L. Del, Cortinas, L., Torres, M., Ponce, F., Petersen, C. L. Claus Leth, Appel, J., Kjaer, A., Awadalla, H. Hany, Hamed, R., Jamal, M., Rayan, M., Mahmoodi, A., Awaad, O., Demerdash, S. El, Adel, A., Kader, M. Abdel, Sarda-Mantel, L. Laure, Michel, J. B., Rouzet, F., Martet, G., Louedec, L., Raguin, O., Vrigneaud, J. M., Saumon, G., Khaw, B. A., Guludec, D. Le, Delahaye, N. Nicolas, Dinanian, S., Slama, M. S., Sarda, L., Tamas, C., Samuel, D., Adams, D., Syrota, A., Moralidis, E. Efstratios, Spyridonidis, T., Arsos, G., Karatzas, N., Karakatsanis, K., Koutelou, M. Maria, Theodorakos, A., Kollaros, N., Manginas, A., Graphakos, S., Tsapaki, V., Kouzoumi, A., Cokkinos, D., Goussetis, E., Prassopoulos, V. Vassilios, Parthenakis, F., Patrianakos, A., Velidaki, A., Koukouraki, S., Papadimitriou, E., Diakakis, G., Maurakis, H., Karakavitsas, N., Vardas, P., Koszegi, Z. Zsolt, Jenei, C., Varga, J., Galuska, L., Edes, I., Ray, Soumendranath, Kundu Bijon, Sarma S K., Day N R., Skouse, D. Douglas, Florimonte, L., Maffioli, L., Piatti, L., Calegari, M., Mancini, R., Bossi, M., Carboni, G. P. Gian Piero, Toriyama, T., Takase, H. Hiroyuki, Okado, T, Tanaka, S., Dohi, Y., Yasuhi, W. Watanabe, Akihiro, S., Akio, Y., Yasunori, W., Kimio, T., Nariaki, E., Yasuhiko, T., Ryo, K., Kasama, S. Shu, Honjo, T., Ichikawa, S., Toyama, T., Kurabayashi, M., Furuhashi, T. Tatsuhiko, Moroi, M., Kunimasa, T., Fukuda, H., Sugi, K., Takeishi, Y. Yasuchika, Arimoto, T., Kubota, I., Kambara, N. Naoshige, Hosokawa, R., Ohba, M., Saito, K., Motomura, H., Kanoi, T., Kume, N., Saji, H., Kita, T., Nohara, R., Akashi, Y. J. Yoshihiro, Musha, H., Kida, K., Suzuki, K., Inoue, K., Kawasaki, K., Hashimoto, N., Miyake, F., Yokoyama, Y. Yasuhiro, Sato, A., Nozato, T., Takahashi, A., Isobe, M., Aonuma, K., Hiroe, M., Peovska, I. Irena, Bosevski, M., Pavlovic, J.Maksimovic, Vavlukis, M., Vallejo, E. Enrique, Rodriguez, G., Jimenez, L., Hernandez, S., Bialostozky, D., Orea, A. Arturo, Asensio, E., Castillo, L., Dorantes, J., Narvaez, R., Gonzalez, O., Zee, P. M. Marc Van der, Verberne, H. J., Straalen, J. P. Van, Fischer, J. C., Sanders, G. T. B., Eck-Smit, B. L. F. Van, Winter, R. J. De, Kostkiewicz, M. Magdalena, Szot, W., Mura, A., Lesniak-Sobelga, A., Olszowska, M., Tracz, W., Ferreira, M. J. Maria Joao, Ferrer-Antunes, A. I., Rodrigues, V., Santos, F., Lima, J., Cerqueira, M. D., Providencia, L. A., Rodrigues, V., Martins, E., Vasconcelos, M. Mariana, Faria, T., Oliveira, A., Garcia, M., Pereira, J., Rocha-Goncalves, F., Lourenço, C. Cândida, Ferrer-Antunes, A., Vieira, H., Martins, R., Franco, F., Providência, L. A., Flotats, A. Albert, Estorch, M., Estorch, M. Montserrat, Mena, E., Camacho, V., Fuertes, J., Rodriguez, A., Flotats, A., Hernandez, M. A., Kulisewsky, J., Carrio, I., Jose-Galcera, J., Caballero-Martínez, A., Jara-Perez, P., Villegas-Garcia, M., Iñigo-García, L., Rosa, J. A. Nuño de la, Florenciano-Sánchez, R., Castillo-Soria, J. F., Valentí-Aldeguer, J. A., Pico-Aracil, F., Martínez-Caballero, A., Villegas-García, M., Castillo-Soria, F., Sánchez-Villanueva, J. G., Rosa, J. A. Nuço de la, Içigo-García, L., Palop, R., Florenciano-Sanchez, R., Lopez-Martínez, I., Jara-Pñrez, P., Rosa, J. A. Nuno de la, Castillo-Soria, F. J., Jara-Pérez, P., Contreras, J., Villegas-Sánchez, M., Obrador, D., Puig, M., Serra-Grima, R., Santaló, M., Carrió, I., Sorensen, J. Jens, Frostfeldt, G., Lindahl, B., Valind, S., Wallentin, L., Somsen, G. A. Aernout, Burri, H., Zaza, S., Fleury, E., Righetti, A., Entok, E. Emre, Birdane, A., Cavusoglu, Y., Ak, I., Timuralp, B., Haworth, M. J., Mitchell, A. R. J. Andrew, Gardner, M. A., Ormerod, O. J. M., Clements, I. P. Ian, Mullan, B. P., MacGregor, C. G., Breen, J. F., Akinboboye, O. O., Nichols, K. J., Wang, Y., Dim, U. Uzodinma, Lachman, J., Nichols, K. J., Muratore, K. A., Aracely, N., Haag, E. S., Druz, R. S. Regina, Gopal, A. S., Borges, A., Ngai, K. C., Dim, U. R., Wiley, M., Freeman, J., Kitchen, S., Graham, D., Schindel, M., Clark, E., Speiser, D., Hackney, T., Feldkamp, M., and Rosamond, T. Thomas

Published
2005
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8. Poster display II clinical general

Author

Gurgenyan, S., Vatinyan, S., Nikogosyan, K., Edilyan, L., Chobanyan, B., Lacourcière, Y., Marcel Dumont, M., Lefebvre, J., Poirier, L., Côté, C., Peix, A., Alonso, O., Chae, I., Chung, J., Gutierrez, C., Kropp, J., Onsel, C., Silvasi, I., Llerena, L., Padhy, A., Garcia-Barreto, D., Trapaga, A., Asen, L., Infante, O., Ponce, F., Cabrera, L., Valiente, J., Tornes, F., Guerrero, I., Zayas, R., ones, M., Castro, J., Fayad, Y., Carrillo, R., Paz, A., Mehlsen, J., Hædersdal, C., Daou, D., Benada, A., Lebtahi, R., Idy-Peretti, I., Guludec, D., Coaguila, C., Vilain, D., Leenhardt, A., Heinicke, N., Benesch, B., Kaiser, T., Seegmüller, M., Schönberger, J., Eilles, C., Riegger, G., Holmer, S., Luchner, A., Kouris, N., Kontogianni, D., Goranitou, G., Sifaki, M., Kalkandi, E., Grassos, H., Papoulia, E., Babalis, D., Moralidis, E., Spyridonidis, T., Arsos, G., Karakatsanis, K., Karatzas, N., Parameswaran, R., Sundaram, P., Padma, S., Haridas, K., Zachariah, M., Kumar, S., Feola, M., Leonardi, G., Peano, S., Bianchi, A., Dutto, P., Guala, E., Biggi, A., Uslenghi, E., Filardi, P., Pace, L., Dellegrottaglie, S., Corrado, L., Cafiero, M., Camerino, R., Maglione, A., Polimeno, M., Zarrilli, A., Chiariello, M., Giorgetti, A., Gimelli, A., Marini, C., Schluter, M., Kusch, A., D'Aragona, I., Marzullo, P., Stanislao, M., Zanco, P., Inglese, E., Bertelli, P., Valle, G., Tassone, F., Pepino, R., Francini, A., Garrone, O., Occelli, M., Merlano, M., Florimonte, L., Pagani, L., Piatti, L., Butti, I., Maffioli, L., Casorelli, E., Dottore, F., Gentili, G., Agostini, M., Pieri, P., Milan, E., Giubbini, R., Mazzanti, M., Serenelli, M., Perna, G., Ferro, A., Duilio, C., Santomauro, M., Salvatore, M., Cuocolo, A., Bertagna, F., Bosio, G., Terzi, A., Paghera, B., Kaneta, T., Otani, H., Hakamatsuka, T., Fukuda, H., Nakazato, R., Moroi, M., Kunimasa, T., Furuhashi, T., Sugi, K., Yasuhi, W., Akihiro, S., Yukawa, A., Ryu, K., Kimio, T., Yasuhiko, T., Nariaki, E., Yasunori, W., Akashi, Y., Musha, H., Kida, K., Itoh, K., Inoue, K., Kawasaki, K., Hashimoto, N., Nakazawa, K., Miyake, F., Fukuzawa, S., Ozawa, S., Inagaki, M., Sugioka, J., Okino, S., Matsuo, S., Matsumoto, T., Nakae, I., Masuda, D., Horie, M., Mori, Y., Takahashi, K., Masai, M., Kawasaki, D., Kanemori, T., Okuda, S., Tanabe, K., Ohyanagi, M., OKuda, S., Toyama, T., Hoshizaki, H., Seki, R., Isobe, N., Kawaguchi, R., Oshima, S., Taniguchi, K., Nakagawa, K., Sekine, T., Yamazaki, M., Komuro, I., Kim, K., Teramoto, N., Jino, H., Ohta, Y., Watabe, H., Hayashi, T., Iida, H., Nishimura, T., Nagae, A., Morishima, K., Shigeyama, T., Shimoyama, K., Yoshino, H., Kawai, Y., Jeong, S., Lee, J., Seo, J., Bae, J., Ahn, B., Chae, S., Lee, K., Cho, I., Chun, K., Won, K., Lee, H., Hong, G., Park, J., Shin, D., Kim, Y., Shim, B., Pavlovic, J., Peovska, I., Vavlukis, M., Gorceva, D., Majstorov, V., Alexanderson, E., Meave, A., Ricalde, A., Teresinska, A., Sliwinski, M., Konieczna, S., Szymanska, M., Hendzel, P., Juraszynski, Z., Debski, A., Szumilak, B., Kostkiewicz, M., Wilkolek, P., Pasowicz, M., Klimeczek, P., Pieniazek, P., Przewlocki, T., Pieculewicz, M., Tracz, W., Szot, W., Trebacz, J., Zmudka, K., Podolec, P., Dziuk, M., Kazmierczak, A., Kot, E., Pietrzykowski, J., Cholewa, M., Coutinho, M., Correia, M., Cantinho, G., Conceição, I., Bernardes, A., Silva, A., Gaspar, F., Cunha, J., Lourenço, C., Roque, C., Ferrer-Antunes, A., Ferreira, M., Providência, L., Lima, J., Abreu, A., Castillejos, L., Henriksson, I., Oliveira, L., Rosário, L., Geão, A., Pereira, E., Colarinha, P., Romero-Farina, G., Candell-Riera, J., Aguadé-Bruix, S., Leon, G., Caresia, A., Mila-Lopez, M., Garcia-Alonso, C., Pifarre-Montaner, P., Negre-Buso, M., Castell-Conesa, J., Mestre-Fusco, A., Porta-Biosca, F., Muxi, A., Paredes, P., Ortin, J., Duch, J., Diaz-Infante, E., Fuertes, S., Orus, J., Mont, L., Pons, F., Pollack, C., Hellermann, J., Namdar, M., Siegrist, P., Koepfli, P., Bartenstein, N., Schurr, U., Jenni, R., Kaufmann, P., Hassad, R., Hamami, H., Sellem, A., Brahim, H., Caglar, M., Mahmoudian, B., Aytemir, K., Kahraman, S., Arýcý, M., Kabakcý, G., Karabulut, E., Akincioglu, C., Berman, D., Nishina, H., Hayes, S., Kavanagh, P., Friedman, J., Slomka, P., Germano, G., Entok, E., Cavusoglu, Y., Vardareli, E., Timuralp, B., Cheetham, A., Naylor, V., Ghiotto, F., McGhie, J., Al-Housni, M., Kelion, A., Hutchings, F., Hinton-Taylor, S., Birkbeck, P., Thatikonda, S., Feldkamp, M., Rosamond, T., Raza, M., Panjrath, G., Haider, A., Jain, D., Yang, A., Schumacher, R., Reynolds, J., Clark, E., Speiser, D., Schindel, M., Hackney, T., Vacek, J., Jindal, V., Dim, U., Hamburg, L., Mouradian, V., Nichols, K., Akinboboye, O., Snyder, K., Polepalle, D., DePuey, G., Khattak, H., Friedman, M., Thompson, L., Thompson, R., McGhie, A., Moser, K., O'Keefe, J., Fritsch, N., Bateman, T., Mut, F., Vidal, I., Rener, A., Nuñez, M., Alvarez, B., and Beretta, M.

Published
2018

10. Trends in microbiological epidemiology of orthopedic infections: a large retrospective study from 2008 to 2021

Author

Boyong Wang, Qiaojie Wang, Musha Hamushan, Jinlong Yu, Feng Jiang, Mingzhang Li, Geyong Guo, Jin Tang, Pei Han, and Hao Shen

Subjects
Orthopedic infections, Microbiological epidemiology, Antimicrobial susceptibilities, Retrospective study, Regional characteristics, Infectious and parasitic diseases, RC109-216
Abstract

Abstract Background This study assessed the distribution characteristics of pathogens isolated from cases of orthopedic infections and focused on the antimicrobial susceptibility of the main pathogens. Methods This retrospective study involved patients with orthopedic infection in a tertiary medical center located in Shanghai, China, from 2008 to 2021.Pathogen information and the basic information of patients were identified from clinical microbiology laboratory data and the institutional medical record system. Results In total, the pathogen information of 2821 patients were enrolled in the study. S. aureus (37.71%) was the main causative pathogen responsible for orthopedic infection. Gender, pathogens distribution and polymicrobial infection rates were significantly different (P

Published
2023
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11. Single-cell transcriptome reveals Staphylococcus aureus modulating fibroblast differentiation in the bone-implant interface

Author

Jinlong Yu, Boyong Wang, Feiyang Zhang, Zun Ren, Feng Jiang, Musha Hamushan, Mingzhang Li, Geyong Guo, and Hao Shen

Subjects
Staphylococcus aureus, scRNA-seq, Fibroblast, Implant-associated infection, Therapeutics. Pharmacology, RM1-950, Biochemistry, QD415-436
Abstract

Abstract Background This study aimed to delineate the cell heterogeneity in the bone-implant interface and investigate the fibroblast responses to implant-associated S. aureus infection. Methods Single-cell RNA sequencing of human periprosthetic tissues from patients with periprosthetic joint infection (PJI, n = 3) and patients with aseptic loosening (AL, n = 2) was performed. Cell type identities and gene expression profiles were analyzed to depict the single-cell landscape in the periprosthetic environment. In addition, 11 publicly available human scRNA-seq datasets were downloaded from GSE datasets and integrated with the in-house sequencing data to identify disease-specific fibroblast subtypes. Furthermore, fibroblast pseudotime trajectory analysis and Single-cell regulatory network inference and clustering (SCENIC) analysis were combined to identify transcription regulators responsible for fibroblast differentiation. Immunofluorescence was performed on the sequenced samples to validate the protein expression of the differentially expressed transcription regulators. Results Eight major cell types were identified in the human bone-implant interface by analyzing 36,466 cells. Meta-analysis of fibroblasts scRNA-seq data found fibroblasts in the bone-implant interface express a high level of CTHRC1. We also found fibroblasts could differentiate into pro-inflammatory and matrix-producing phenotypes, each primarily presented in the PJI and AL groups, respectively. Furthermore, NPAS2 and TFEC which are activated in PJI samples were suggested to induce pro-inflammatory polarization in fibroblasts, whereas HMX1, SOX5, SOX9, ZIC1, ETS2, and FOXO1 are matrix-producing regulators. Meanwhile, we conducted a CMap analysis and identified forskolin as a potential regulator for fibroblast differentiation toward matrix-producing phenotypes. Conclusions In this study, we discovered the existence of CTHRC1 + fibroblast in the bone-implant interface. Moreover, we revealed a bipolar mode of fibroblast differentiation and put forward the hypothesis that infection could modulate fibroblast toward a pro-inflammatory phenotype through NPAS2 and TFEC.

Published
2023
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12. Identification of Staphylococcus aureus virulence-modulating RNA from transcriptomics data with machine learning

Author

Jinlong Yu, Mingzhang Li, Jin Wang, Musha Hamushan, Feng Jiang, Boyong Wang, Yujie Hu, Pei Han, Jin Tang, Geyong Guo, and Hao Shen

Subjects
machine learning, Staphylococcus aureus, sRNA, independent component analysis, RNA-seq, Infectious and parasitic diseases, RC109-216
Abstract

ABSTRACTThe virulence factors of Staphylococcus aureus are tightly controlled by two-component systems (TCSs) and small RNA (sRNA). TCSs have been well studied over the past several decades, but our understanding of sRNA functions lags far behind that of TCS functions. Here, we studied the biological role of sRNA from 506 S. aureus RNA-seq datasets using independent component analysis (ICA). We found that a previously neglected sRNA, Sau-41, functions in the Agr system. Sau-41 is located within the PSMα operon and controlled by the Agr system. It was predicted to share 22-base complementarity with RNAIII, a major regulator of S. aureus virulence. The EMSA results demonstrated that Sau-41 directly binds to RNAIII. Furthermore, our results found that Sau-41 is capable of repressing S. aureus haemolysin activity by downregulating α-haemolysin and δ-toxin. The repression of α-haemolysin was attributed to the competition between the 5’ UTR of hla and Sau-41 for binding RNAIII. We observed that Sau-41 mitigated S. aureus virulence in an orthopaedic implant infection mouse model and alleviated osteolysis. Together, our results indicate that Sau-41 is a virulence-regulating RNA and suggest that Sau-41 might be involved in a negative feedback mechanism to control the Agr system. This work is a demonstration of using ICA in sRNA identification by mining high-throughput data and could be extended to other organisms as well.

Published
2023
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14. Role of Perforating Artery Pedicled Neurotrophic Flap in the Treatment of Compound Tissue Defect of Tibia Using the Ilizarov Technique

Author

Yuan‐jian Wu, Yu‐bo Zhang, Musha Hamushan, Wen‐jun Zhang, Tao Zhang, Xu‐jun Li, Pei Han, and Teng‐fei Lou

Subjects
Compound defect, Ilizarov technique, Pedicled flap, Reconstruction, Tibia, Orthopedic surgery, RD701-811
Abstract

Objective To describe our experience with the combined use of pedicled neurotrophic flap and distraction osteogenesis in the management of complex lower extremity injuries with composite bone and soft tissue defects and assess the functional and cosmetic results of this method. Methods A pedicled flap with a marked perforator artery was applied for soft tissue coverage after radical debridement and temporary external fixation. In the second stage, the Ilizarov external fixator was used in place of the temporary external fixator for reconstruction of the segmental bone defect by distraction osteogenesis. Twenty‐five patients (16 men and nine women; mean age, 39.2 years) were treated by using this combined technique between 2008 and 2016. All cases were graded initially as Gustilo–Anderson grade IIIB open fractures. The soft tissue defect after radical debridement ranged from 9 cm × 5 cm to 14 cm × 11 cm, and the average size of segmental defect was 5.2 (Range, 2.5–8.5) cm. Seventeen of these patients had a history of local infection. The bone structure and function were evaluated by two independent evaluators using Paley's criteria. Results Twenty‐five patients were followed up for an average of 28.96 (Range, 15–48) months. The distally based sural neurovascular flap was applied in 13 patients, and the greater saphenous neurocutaneous perforator flap in 12 patients. The flap area ranged from 10 cm × 5 cm to 14 cm × 12 cm. Sufficient coverage of soft tissue defect was achieved in all cases. All flaps survived completely without complications. The bone defects were corrected by a mean lengthening of 6.94 (Range, 4.5–9.5) cm. The residual discrepancy was

Published
2022
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16. 46P Identification of novel biomarkers distinguishing pancreatic head cancer from distal cholangiocarcinoma discovered by proteomics analysis

Author

Takenami, T., primary, Maeda, S., additional, Karasawa, H., additional, Mochizuki, Y., additional, Aizawa, T., additional, Jun, S., additional, Musha, H., additional, Morikawa, T., additional, Nakagawa, K., additional, Hayashi, H., additional, Motoi, F., additional, Naitoh, T., additional, and Unno, M., additional

Published
2016
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20. 2088 Clinical features and management of anorectal cancer in Crohn's disease: Japanese single centre study

Author

Watanabe, K., primary, Nagao, M., additional, Abe, T., additional, Imoto, H., additional, Tanaka, N., additional, Kudo, K., additional, Aoki, T., additional, Karasawa, H., additional, Tsuchiya, T., additional, Kobayashi, M., additional, Mizuma, M., additional, Hayashi, H., additional, Morikawa, T., additional, Nakagawa, K., additional, Sakata, N., additional, Ohnuma, S., additional, Musha, H., additional, Motoi, F., additional, Naitoh, T., additional, and Unno, M., additional

Published
2015
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22. High-purity magnesium pin enhances bone consolidation in distraction osteogenesis via regulating Ptch protein activating Hedgehog-alternative Wnt signaling

Author

Musha Hamushan, Weijie Cai, Yubo Zhang, Zun Ren, Jiafei Du, Shaoxiang Zhang, Changli Zhao, Pengfei Cheng, Xiaonong Zhang, Hao Shen, and Pei Han

Subjects
Materials of engineering and construction. Mechanics of materials, TA401-492, Biology (General), QH301-705.5
Abstract

Magnesium alloys are promising biomaterials for orthopedic implants because of their degradability, osteogenic effects, and biocompatibility. Magnesium has been proven to promote distraction osteogenesis. However, its mechanism of promoting distraction osteogenesis is not thoroughly studied. In this work, a high-purity magnesium pin developed and applied in rat femur distraction osteogenesis. Mechanical test, radiological and histological analysis suggested that high-purity magnesium pin can promote distraction osteogenesis and shorten the consolidation time. Further RNA sequencing investigation found that alternative Wnt signaling was activated. In further bioinformatics analysis, it was found that the Hedgehog pathway is the upstream signaling pathway of the alternative Wnt pathway. We found that Ptch protein is a potential target of magnesium and verified by molecular dynamics that magnesium ions can bind to Ptch protein. In conclusion, HP Mg implants have the potential to enhance bone consolidation in the DO application, and this process might be via regulating Ptch protein activating Hedgehog-alternative Wnt signaling.

Published
2021
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25. A General Map of Transcriptional Expression of Virulence, Metabolism, and Biofilm Formation Adaptive Changes of Staphylococcus aureus When Exposed to Different Antimicrobials

Author

Zun Ren, Jinlong Yu, Jiafei Du, Yubo Zhang, Musha Hamushan, Feng Jiang, Feiyang Zhang, Boyong Wang, Jin Tang, Hao Shen, and Pei Han

Subjects
Staphylococcus aureus, antimicrobials, biofilm formation, virulence, metabolism, Microbiology, QR1-502
Abstract

Biofilm formation of Staphylococcus aureus is the major cause of implant-associated infections (IAIs). Antimicrobial treatment is one of the most effective therapeutic options for S. aureus infections. However, it can also lead to adaptive transcriptomic changes due to extreme selective pressure, which may increase the risk of antimicrobial resistance. To study the transcriptional changes in S. aureus upon exposure to antimicrobial agents, we obtained expression profiles of S. aureus treated with six antimicrobials (flucloxacillin, vancomycin, ciprofloxacin, clindamycin, erythromycin, and linezolid, n = 6 for each group). We also included an untreated control group (n = 8) downloaded from the Gene Expression Omnibus (GEO) database (GSE70043, GSE56100) for integrated bioinformatic analyses. We identified 82 (44 up, 38 down) and 53 (17 up, 36 down) differentially expressed genes (DEGs) in logarithmic and stationary phases, respectively. When exposed to different antimicrobial agents, we found that manganese import system genes and immune response gene sbi (immunoglobulin G-binding protein Sbi) were upregulated in S. aureus at all stages. During the logarithmic phase, we observed adaptive transcriptomic changes in S. aureus mainly in the stability of protein synthesis, adhesion, and biofilm formation. In the stationary phase, we observed a downregulation in genes related to amino biosynthesis, ATP synthesis, and DNA replication. We verified these results by qPCR. Importantly, these results could help our understanding of the molecular mechanisms underlying the proliferation and antimicrobial resistance of S. aureus.

Published
2022
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30. Abstracts

Author

Barthelemy, O., primary, Silvain, J., additional, Brieger, D., additional, Bellemain-Appaix, A., additional, Cayla, G., additional, Beygui, F., additional, Lancar, R., additional, Collet, J. P., additional, Mercadier, A., additional, Montalescot, G., additional, Cha, K. S., additional, Nam, Y. H., additional, Kim, J. H., additional, Park, S. Y., additional, Park, T. H., additional, Kim, M. H., additional, Kim, Y. D., additional, Lee, H. C., additional, Ahn, M. S., additional, Hong, T. J., additional, Blanco, R., additional, Blanco, F., additional, Szarfer, J., additional, Garcia Escudero, A., additional, Gigena, G., additional, Gagliardi, J., additional, Rodriguez, A., additional, Sarmiento, R., additional, Affatatto, S., additional, Riccitelli, M., additional, Petris, A., additional, Datcu, M. D., additional, Pop, C., additional, Radoi, M., additional, Arsenescu-Georgescu, C., additional, Petrescu, I., additional, Petrescu, L., additional, Serban, L., additional, Nechita, E., additional, Tatu-Chitoiu, G., additional, Dorobantu, M., additional, Benedek, I., additional, Craiu, E., additional, Sinescu, C., additional, Ionescu, D. D., additional, Ginghina, C., additional, Minescu, B., additional, Izzo, A., additional, Mantovani, P., additional, Tomasi, L., additional, Dall'oglio, L., additional, Bonatti, S., additional, Rosiello, R., additional, Romano, M., additional, Agostini, F., additional, Zanini, R., additional, Zhao, Z. Y., additional, Wu, Y. J., additional, Li, J. J., additional, Yany, Y. J., additional, Qian, H. Y., additional, Tang, Y. D., additional, Timoteo, A. T., additional, Toste, A., additional, Lousinha, A., additional, Ramos, R., additional, Oliveira, J. A., additional, Ferreira, M. L., additional, Ferreira, R. C., additional, Cabades, C., additional, Diez Gil, J. L., additional, Aguar, P., additional, Sanmiguel, D., additional, Lopez-March, A., additional, Marmol, R., additional, Guerra, L., additional, Girbes, V., additional, Ferrando, J., additional, Rincon De Arellano, A., additional, Patricio, L., additional, Blondal, M., additional, Ainla, T., additional, Marandi, T., additional, Eha, J., additional, Oliveira, M. M., additional, Silva, M. N., additional, Cunha, P. S., additional, Feliciano, J., additional, Silva, S., additional, Kanovsky, J., additional, Kala, P., additional, Parenica, J., additional, Poloczek, M., additional, Prymusova, K., additional, Kubkova, L., additional, Spinar, J., additional, Olinic, D., additional, Homorodean, C., additional, Ober, M., additional, Olinic, M., additional, Andrioaia, C., additional, Condac, A., additional, Masmoudi, M., additional, Berdaoui, B., additional, Labidi, S., additional, Tapia Ballesteros, C., additional, Hernandez Luis, C., additional, Sandin, M. G., additional, Vegas, J. M., additional, Andion, R., additional, Martinez, N., additional, Gonzalez, I. A., additional, Alvarado, M., additional, Amat, I. J., additional, San Roman, J. A., additional, Garcia Gonzalez, M. 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F., additional, Faustino, C., additional, Ariza Sole, A., additional, Sanchez Salado, J. C., additional, Lorente Tordera, V., additional, Martinez Garcia, V., additional, Salazar Mendiguchia Y Garcia, J., additional, Gomez Hospital, J. A., additional, Maristany Daunert, J., additional, Berdejo Gago, F. J., additional, Esplugas Oliveras, E., additional, Brzozowska-Czarnek, A., additional, Urbanik, A., additional, Kakouros, N., additional, Kakouros, S., additional, Lekakis, J., additional, Rizos, J., additional, Kokkinos, D., additional, Venevtseva, J., additional, Melnikov, A., additional, Valiahmetov, M., additional, Gomova, T., additional, Perelomova, I., additional, Ferrer Hita, J. J., additional, Bosa-Ojeda, F., additional, Sanchez-Grande-Flecha, A., additional, Yanes-Bowden, G., additional, Vargas-Torres, M. 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A., additional, Savic, L., additional, Mrdovic, I., additional, Krljanac, G., additional, Perunicic, J., additional, Asanin, M., additional, Lasica, R., additional, Matic, M., additional, Vasiljevic, Z., additional, Ostojic, M., additional, Tichy, M., additional, Andrys, C., additional, Conti, A., additional, Poggioni, C., additional, Viviani, G., additional, Bulletti, F., additional, Boni, V., additional, Luzzi, M., additional, Vicidomini, S., additional, Donati, M., additional, Del Taglia, B., additional, Pini, R., additional, Sousa, O., additional, Fontes-Carvalho, R., additional, Caeiro, D., additional, Dias Ferreira, N., additional, Silva, G., additional, Pereira, E., additional, Ribeiro, J., additional, Albuquerque, A., additional, Gama Ribeiro, V., additional, Murai, M., additional, Takeda, Y., additional, Shinmyo, T., additional, Tanigawa, J., additional, Hazui, H., additional, Nakakohji, T., additional, Ohishi, Y., additional, Hoshiga, M., additional, Ishihara, T., additional, Hanafusa, T., additional, Belohlavek, J., additional, Rohn, V., additional, Kunstyr, J., additional, Lips, M., additional, Semrad, M., additional, Mlejnsky, F., additional, Tosovsky, J., additional, Linhart, A., additional, Lindner, J., additional, Sablik, Z., additional, Samborska-Sablik, A., additional, Drozdz, J., additional, Gaszynski, W., additional, Izquierdo-Gomez, M. 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J., additional, Gomez Salvador, I., additional, San Roman Calvar, J. A., additional, Mamarasulov, T. M., additional, Todorovic, L., additional, Cherneva, Z. C. H., additional, Denchev, S. D., additional, Heltai, K., additional, Boytsov, A., additional, Nikulina, N. N., additional, Zanna, D., additional, Marangelli, V., additional, Caiati, C., additional, Picon Heras, R., additional, Loureiro, M. J., additional, Urazovskaya, I., additional, Vinogradova, D., additional, Vasilieva, E., additional, Shpektor, A., additional, Conti, E., additional, Musumeci, M. B., additional, Lauri, F. M., additional, Dito, E., additional, De Giusti, M., additional, Lallo, A., additional, Fusco, D., additional, Davoli, M., additional, Volpe, M., additional, Autore, C., additional, Gamra, H., additional, Dridi, Z., additional, Hassine, M., additional, Addad, F., additional, Gherissi, I., additional, Reda, A., additional, Mahjoub, M., additional, Bouraoui, S., additional, Abdennadher, M., additional, Betbout, F., additional, Mota, P. M. F. P., additional, Silva, J. D., additional, Jankovic Tomasevic, R., additional, Djordjevic, V., additional, Djordjevic Radojkovic, D., additional, Scafa Udriste, A., additional, Fruntelata, A., additional, Gainoiu, E., additional, Bogdan, S., additional, Zamfir, D., additional, Teodorescu, C., additional, Guran, M., additional, Constantinescu, D., additional, Konopka, A., additional, Banaszewski, M., additional, Wojtkowska, I., additional, Stepinska, J., additional, Vidergold, J. V., additional, Osipova, I. V., additional, Tavrovskaya, T. V., additional, Galkina, J. V., additional, Timofeev, A. V., additional, Vorobyov, R. I., additional, Vorobyova, E. N., additional, Matos, L., additional, Carvalho, A. C. C., additional, Oliveira, W., additional, Cintra, F., additional, Poyares, D., additional, Andersen, M., additional, Martins, R., additional, Tufik, S., additional, Ostadal, P., additional, Brada, J., additional, Horakova, S., additional, Mlcek, M., additional, Hrachovina, V., additional, Kittnar, O., additional, Gorudko, I. V., additional, Buko, I. V., additional, Cherenkevich, S. N., additional, Polonetsky, L. Z., additional, Plotkin, V. Y., additional, Timoshina, M. A., additional, Azanchevskaya, S. V., additional, Chromov-Borisov, N. N., additional, Vorlat, A., additional, Snoep, L., additional, Claeys, M. J., additional, Vrints, C. J., additional, Palazzuoli, A., additional, Caputo, M., additional, Quatrini, I., additional, Calabro, A., additional, Antonelli, G., additional, Campagna, M. S., additional, Franci, B., additional, Nuti, R., additional, Maisel, A., additional, Negrini, M., additional, Minora, T., additional, Marino, P., additional, Seregni, R., additional, Tavlueva, E., additional, Barbarash, O., additional, Barbarash, L., additional, Janota, T., additional, Kudlicka, J., additional, Malik, K., additional, Wichterle, D., additional, Hradec, J., additional, Body, R., additional, Carley, S. D., additional, Mcdowell, G., additional, Nuttall, M., additional, Wibberley, C., additional, France, M., additional, Cruickshank, J. K., additional, Mackway-Jones, K., additional, Leon, M., additional, Cozma, C., additional, Mitu, F., additional, Almeida, D. R., additional, Dias, C. B., additional, Burazor, I., additional, Burazor, M., additional, Krstic, M., additional, Lazovic, M., additional, Vukmanovic, M., additional, Djordjevic, J., additional, Radovanovic, Z., additional, Ilic, D., additional, Bosnjakovic, P., additional, Ferreira, A. C., additional, Mateus, P. 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G., additional, Zatarain Nicolas, E., additional, Martinez Uruena, N., additional, Alvarado Montes De Oca, M., additional, Dytrych, V., additional, Kovarnik, T., additional, Smid, O., additional, Kral, A., additional, Aroutunov, A. G., additional, Intwala, S., additional, Jegere, I., additional, Shaalan, H. S. H., additional, Pagava, Z., additional, Agladze, R., additional, Shakarishvili, R., additional, Sharashidze, N., additional, Gujejiani, L., additional, Saatashvili, G., additional, Katova, T. 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A., additional, Adgey, J. A. A., additional, Caeiro Pereira, D., additional, Braga, P., additional, Fontes Carvalho, R., additional, Rodrigues, A., additional, Goncalves, M., additional, Simoes, L., additional, and Borisov, K. V., additional

Published
2010
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36. Thermonucleases Contribute to Staphylococcus aureus Biofilm Formation in Implant-Associated Infections–A Redundant and Complementary Story

Author

Jinlong Yu, Feng Jiang, Feiyang Zhang, Musha Hamushan, Jiafei Du, Yanjie Mao, Qiaojie Wang, Pei Han, Jin Tang, and Hao Shen

Subjects
Staphylococcus aureus, biofilm, thermonuclease, implant associated infections, periprosthetic joint infection, Microbiology, QR1-502
Abstract

Biofilms formed by Staphylococcus aureus are one of the predominant causes of implant-associated infections (IAIs). Previous studies have found that S. aureus nucleases nuc1 and nuc2 modulate biofilm formation. In this study, we found low nuc1/nuc2 expression and high biofilm-forming ability among IAI isolates. Furthermore, in a mouse model of exogenous IAIs, Δnuc1/2 exhibited higher bacterial load on the surface of the implant than that exhibited by the other groups (WT, Δnuc1, and Δnuc2). Survival analysis of the hematogenous IAI mouse model indicated that nuc1 is a virulence factor related to mortality. We then detected the influence of nuc1 and nuc2 on biofilm formation and immune evasion in vitro. Observation of in vitro biofilm structures with scanning electron microscopy and evaluation of bacterial aggregation with flow cytometry revealed that both nuc1 and nuc2 are involved in biofilm structuring and bacterial aggregation. Unlike nuc1, which is reported to participate in immune evasion, nuc2 cannot degrade neutrophil extracellular traps. Moreover, we found that nuc1/nuc2 transcription is negatively correlated during S. aureus growth, and a possible complementary relationship has been proposed. In conclusion, nuc1/nuc2 are complementary genes involved in biofilm formation in exogenous IAIs. However, nuc2 contributes less to virulence and is not involved in immune evasion.

Published
2021
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37. Chiral Supramolecular Hydrogel Loaded with Dimethyloxalyglycine to Accelerate Chronic Diabetic Wound Healing by Promoting Cell Proliferation and Angiogenesis

Author

Yubo Zhang, Weijie Cai, Zun Ren, Yuxiang Lu, Musha Hamushan, Pengfei Cheng, Zhengyu Xu, Hao Shen, Changli Zhao, Pei Han, and Wanrun Zhong

Subjects
supramolecular hydrogel, chirality, endothelial cells, wound healing, DMOG, Science, Chemistry, QD1-999, Inorganic chemistry, QD146-197, General. Including alchemy, QD1-65
Abstract

Chronic refractory wounds are one of the most serious complications of diabetes, and the effects of common treatments are limited. Chiral hydrogel combined with dimethyloxalyglycine (DMOG) as a dressing is a promising strategy for the treatment of chronic wounds. In this research, we have developed a DMOG-loaded supramolecular chiral amino-acid-derivative hydrogel for wound dressings for full-thickness skin regeneration of chronic wounds. The properties of the materials, the ability of sustained release drugs, and the ability to promote angiogenesis were tested in vitro, and the regeneration rate and repair ability of full-thickness skin were tested in vivo. The chiral hydrogel had the ability to release drugs slowly. It can effectively promote cell migration and angiogenesis in vitro, and promote full-thickness skin regeneration and angiogenesis in vivo. This work offers a new approach for repairing chronic wounds completely through a supramolecular chiral hydrogel loaded with DMOG.

Published
2022
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42. 123I-BMIPP delayed scintigraphic imaging in patients with chronic heart failure.

Author

Kida K, Akashi YJ, Yoneyama K, Shimokawa M, Musha H, Kida, Keisuke, Akashi, Yoshihiro J, Yoneyama, Kihei, Shimokawa, Mitsuhiro, and Musha, Haruki

Abstract

Objective: The objective of the present study was to clarify the ability of 123I-beta-methyl-iodophenylpentadecanoic acid (123I-BMIPP) to evaluate the heart-to-mediastinum (H/M) ratio and myocardial global washout rate (WR) in patients with chronic heart failure (CHF). Methods: The severity of CHF was evaluated on the basis of the New York Heart Association (NYHA) classification. Twenty patients with CHF (13 with idiopathic dilated cardiomyopathy and 7 with ischemic cardiomyopathy) and 11 age-matched controls underwent myocardial radionuclide imaging. Scintigraphic images were obtained from each participant at the early (30 min following radio-isotope injection) and late (4 h) phases using 123I-BMIPP. The H/M ratio and WR were calculated from planar images. Concentrations of plasma brain natriuretic peptide (BNP) were measured prior to the scintigraphic study. Results: The 123I-BMIPP uptake of early H/M and global WR did not significantly differ among groups, but uptake of delayed H/M was significantly lower in patients with NYHA class III than in controls (control 2.47 +/- 0.39; class III 1.78 +/- 0.28, P < 0.05). The uptake of delayed H/M and global WR correlated with plasma log BNP in all participants (r = -0.38, P < 0.05; 0.43, P < 0.05, respectively). Conclusions: These data suggest that 123I-BMIPP uptake of delayed H/M enhances the image of CHF severity. The myocardial WR of 123I-BMIPP also effectively depicted the severity of CHF. [ABSTRACT FROM AUTHOR]

Published
2008
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43. Decision for Allowing Patients With Heart Disease to Participate in Sports.

Author

Musha, H.

Abstract

Doctors can be asked to decide whether a patient should participate in sports in order to avoid health problems. However, safeguarding a patient's health may sometimes impose excessive restrictions and may impair the quality of life for sport-loving patients. I will introduce two cases that demonstrate the criteria for deciding whether patients with heart disease can participate in sports. Case 1. The patient was a 67-year-old man who was found to have ST depression during a medical checkup. He was a keen jogger and took part in a 10km municipal marathon competition. Recently, he noticed the occurrence of angina-like symptoms soon after starting to run. Investigations revealed that he developed ST depression during an exercise tolerance test. The patient had 75% stenosis of the right coronary artery (segment #2), as well as 90%, 90%, and 100% stenosis of segments #6, #7, and #9, respectively, in the left coronary artery. Coronary angioplasty was indicated. Three stents were placed in #2, #6 and #7, and revascularization was achieved. However, #9 remained 100% stenosis. Recommendations for participants in a marathon include a criterion concerning coronary artery disease, which reads "A person with a history of bypass surgery or coronary angioplasty may join a marathon if he/she has obtained complete revascularization". Therefore, this patient was allowed to go jogging, but was not given approval to take part in a marathon because he had residual coronary stenosis. Case 2: The patient was a 62-year-old man who showed ST-T abnormalities on an ECG recorded during health screening while he was asymptomatic. The patient liked to play golf and usually did so every week, completing one round of the course each time. He was diagnosed as having hypertrophic cardiomyopathy, based on echocardiography and MRI findings. Holter ECG monitoring revealed non-sustained ventricular tachycardia (several successive beats). The recommendation about cardiomyopathy patients playing sports is as follows . "Cardiomyopathy patients are recommended not to participate in competitive sports, regardless of whether they are symptomatic or asymptomatic and the type of disease, but can play class 1A sports for recreation". Therefore, because non-competitive golf utilizing a cart is not stressful exercise, the patient was given approval to play golf for his enjoyment. [ABSTRACT FROM AUTHOR]

Published
2008

44. Induction of automated external defibrillator (AED) to prevent sudden cardiac death in sports activities.

Author

Musha, H.

Abstract

Many sudden deaths including those in sports activities occur by lethal ventricular arrhythmias, for which cardioversion is the only method of treatment. Basic life support (BLS)embracing cardioversion by bystanders is the most effective for preventing sudden death. In the U.S.A., public access defibrillation(PAD) where a citizen performs cardioversion as a part of BLS is accepted legally, and automated external defibrillators(AED) are deployed in airports and locations with large public gatherings. The machinery and tools can by used by the average citizen, and AED does not require an electrocardiogram reading or medical technical knowledge, and operation is possible with a sound guide. In the U.S.A., the Good Samaritan Law was enacted to allow the average citizen to use AED, but in Japan, the government has just begun talks to introduce legislation that will allow persons other than doctors and co medical staff to use the AED within 2004. [ABSTRACT FROM AUTHOR]

Published
2004

47. Marked delay in indocyanine green plasma clearance with a near-normal bromosulphophthalein retention test: a constitutional abnormality?

Author

Okuda, K, Ohkubo, H, Musha, H, Kotoda, K, Abe, H, and Tanikawa, K

Abstract

Five patients showing a normal to near-normal BSP test and a marked delay in ICG plasma clearance, and two families with clustering of the same abnormality, are described. Two had Gilbert's syndrome, one was convalescing from acute hepatitis, and the other two had no detectable liver abnormality. Measured indices of ICG metabolism indicated a marked reduction in the hepatic uptake, storage capacity, biliary transport maximum, and an increased reflux into plasma. Biochemical studies on the binding of ICG by plasma proteins failed to demonstrate any difference from normal controls. Thus, the primary defect seems to be in the transport of ICG by the hepatocyte. Although the relationship of the defect in ICG metabolism to other constitutional hyperbilirubinaemias is not clear, it is possible that the defect in these patients is a constitutional one involving some steps in the hepatic disposal of organic anions. [ABSTRACT FROM PUBLISHER]

Published
1976

50. Sonographic detection of large spontaneous spleno-renal shunts and its clinical significance

Author

Takayasu, K., Moriyama, N., Shima, Y., Yamada, T., Kobayashi, C., Musha, H., and Okuda, K.

Abstract

The ultrasonographic findings of large spleno-renal shunts seen in five patients with liver cirrhosis and confirmed by CT and/or portography, are described. These spleno-renal shunts were seen in the left inter- or subcostal sections as transonic, mass-like lesions located between the splenic hilum and the left kidney, and by continuous scanning, they were found to be part of a tortuous, large tubular structure. Oesophageal varices were absent or minimal in these patients, and none of them had ever bled.

Published
1984
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