1. The extracellular matrix differentially directs myoblast motility and differentiation in distinct forms of muscular dystrophy: Dystrophic matrices alter myoblast motility.
- Author
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Long, Ashlee, Kwon, Jason, Lee, GaHyun, Reiser, Nina, Vaught, Lauren, OBrien, Joseph, Page, Patrick, Hadhazy, Michele, Demonbreun, Alexis, McNally, Elizabeth, Crosbie, Rachelle, and Reynolds, Joseph
- Subjects
Annexin ,Duchenne muscular dystrophy ,Dysferlin ,Extracellular matrix ,Limb girdle muscular dystrophy ,Muscle ,Myoblast ,Animals ,Myoblasts ,Extracellular Matrix ,Mice ,Cell Differentiation ,Sarcoglycans ,Cell Movement ,Dysferlin ,Muscular Dystrophies ,Dystrophin ,Annexin A2 ,Decorin ,Cell Line ,Disease Models ,Animal ,Muscle ,Skeletal - Abstract
Extracellular matrix (ECM) pathologic remodeling underlies many disorders, including muscular dystrophy. Tissue decellularization removes cellular components while leaving behind ECM components. We generated on-slide decellularized tissue slices from genetically distinct dystrophic mouse models. The ECM of dystrophin- and sarcoglycan-deficient muscles had marked thrombospondin 4 deposition, while dysferlin-deficient muscle had excess decorin. Annexins A2 and A6 were present on all dystrophic decellularized ECMs, but annexin matrix deposition was excessive in dysferlin-deficient muscular dystrophy. Muscle-directed viral expression of annexin A6 resulted in annexin A6 in the ECM. C2C12 myoblasts seeded onto decellularized matrices displayed differential myoblast mobility and fusion. Dystrophin-deficient decellularized matrices inhibited myoblast mobility, while dysferlin-deficient decellularized matrices enhanced myoblast movement and differentiation. Myoblasts treated with recombinant annexin A6 increased mobility and fusion like that seen on dysferlin-deficient decellularized matrix and demonstrated upregulation of ECM and muscle cell differentiation genes. These findings demonstrate specific fibrotic signatures elicit effects on myoblast activity.
- Published
- 2024