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14. Cell Biological Assays for Measuring Odontogenic Activities of CCN Proteins.

Author

Muromachi K, Sugiya H, and Tani-Ishii N

Subjects
Blotting, Western, CCN Intercellular Signaling Proteins genetics, Cells, Cultured, Connective Tissue Growth Factor genetics, Connective Tissue Growth Factor metabolism, Dental Pulp cytology, Dental Pulp metabolism, Gene Expression, Humans, Immunohistochemistry, Odontoblasts drug effects, Odontoblasts metabolism, Odontogenesis genetics, Peroxidase metabolism, Biological Assay, CCN Intercellular Signaling Proteins metabolism, CCN Intercellular Signaling Proteins pharmacology, Odontogenesis drug effects
Abstract

In postnatal dentin formation, odontoblast differentiation occurs in the pulp tissue regenerative process under pathological condition. Odontoblasts and newly differentiated odontoblast-like cells beneath the caries lesion form tertiary dentin and are highly odontogenic. To observe the activity of dentinogenesis occur within the hard tissue, a combination of immunohistological analysis and immunodetection of dentinogenesis specific molecules, such as dentin sialophosphoprotein (DSPP) and/or its cleaved products dentin sialoprotein (DSP) and dentin phosphoprotein (DPP), is a reliable approach. Besides, recent studies have revealed that the expression of CCN family member 2 (CCN2), a member of the CCN family protein, is confirmed in accordance with tooth development and reparative dentin formation. Therefore, CCN2 could serve as a marker for dentinogenesis. Here, we describe a method for visualizing the CCN2 signal as an odontogenic activity in formalin-fixed paraffin-embedded (FFPE) sections of demineralized human teeth and human dental pulp cells.

Published
2017
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