1. Experimental conical-head abutment screws on the microbial leakage through the implant-abutment interface: an in vitro analysis using target-specific DNA probes.
- Author
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Pita, Murillo S., Nascimento, Cássio, Santos, Carla G. P., Pires, Isabela M., and Pedrazzi, Vinícius
- Subjects
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DENTAL abutments , *ORAL microbiology , *DENTAL screws , *DENTAL implant complications , *LEAKAGE , *HUMAN DNA , *TOOL design & construction - Abstract
Objectives The aim of this in vitro study was to identify and quantify up to 38 microbial species from human saliva penetrating through the implant-abutment interface in two different implant connections, external hexagon and tri-channel internal connection, both with conventional flat-head or experimental conical-head abutment screws. Material and methods Forty-eight two-part implants with external hexagon ( EH; n = 24) or tri-channel internal ( TI; n = 24) connections were investigated. Abutments were attached to implants with conventional flat-head or experimental conical-head screws. After saliva incubation, Checkerboard DNA- DNA hybridization was used to identify and quantify up to 38 bacterial colonizing the internal parts of the implants. Kruskal-Wallis test followed by Bonferroni's post-tests for multiple comparisons was used for statistical analysis. Results Twenty-four of thirty-eight species, including putative periodontal pathogens, were found colonizing the inner surfaces of both EH and TI implants. Peptostreptococcus anaerobios ( P = 0.003), Prevotella melaninogenica ( P < 0.0001), and Candida dubliniensis ( P < 0.0001) presented significant differences between different groups. Means of total microbial count (×104, ± SD) for each group were recorded as follows: G1 (0.27 ± 2.04), G2 (0 ± 0), G3 (1.81 ± 7.50), and G4 (0.35 ± 1.81). Conclusions Differences in the geometry of implant connections and abutment screws have impacted the microbial leakage through the implant-abutment interface. Implants attached with experimental conical-head abutment screws showed lower counts of microorganisms when compared with conventional flat-head screws. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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