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27 results on '"Murgoci, Adriana-Natalia"'

5. ARG1-expressing microglia show a distinct molecular signature and modulate postnatal development and function of the mouse brain

Author

Stratoulias, Vassilis, primary, Ruiz, Rocío, additional, Kanatani, Shigeaki, additional, Osman, Ahmed M., additional, Keane, Lily, additional, Armengol, Jose A., additional, Rodríguez-Moreno, Antonio, additional, Murgoci, Adriana-Natalia, additional, García-Domínguez, Irene, additional, Alonso-Bellido, Isabel, additional, González Ibáñez, Fernando, additional, Picard, Katherine, additional, Vázquez-Cabrera, Guillermo, additional, Posada-Pérez, Mercedes, additional, Vernoux, Nathalie, additional, Tejera, Dario, additional, Grabert, Kathleen, additional, Cheray, Mathilde, additional, González-Rodríguez, Patricia, additional, Pérez-Villegas, Eva M., additional, Martínez-Gallego, Irene, additional, Lastra-Romero, Alejandro, additional, Brodin, David, additional, Avila-Cariño, Javier, additional, Cao, Yang, additional, Airavaara, Mikko, additional, Uhlén, Per, additional, Heneka, Michael T., additional, Tremblay, Marie-Ève, additional, Blomgren, Klas, additional, Venero, Jose L., additional, and Joseph, Bertrand, additional

Published
2023
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7. ARG1-expressing microglia show a distinct molecular signature and modulate postnatal development and function of the mouse brain

Author

Stratoulias, Vassilis, Ruiz, Rocío, Kanatani, Shigeaki, Osman, Ahmed M., Keane, Lily, Armengol, Jose A., Rodríguez-Moreno, Antonio, Murgoci, Adriana-Natalia, García-Domínguez, Irene, Alonso-Bellido, Isabel, González Ibáñez, Fernando, Picard, Katherine, Vázquez-Cabrera, Guillermo, Posada-Pérez, Mercedes, Vernoux, Nathalie, Tejera, Dario, Grabert, Kathleen, Cheray, Mathilde, González-Rodríguez, Patricia, Pérez-Villegas, Eva M., Martínez-Gallego, Irene, Lastra-Romero, Alejandro, Brodin, David, Avila-Cariño, Javier, Cao, Yang, Airavaara, Mikko, Uhlén, Per, Heneka, Michael T., Tremblay, Marie-Ève, Blomgren, Klas, Venero, Jose L., Joseph, Bertrand, Stratoulias, Vassilis, Ruiz, Rocío, Kanatani, Shigeaki, Osman, Ahmed M., Keane, Lily, Armengol, Jose A., Rodríguez-Moreno, Antonio, Murgoci, Adriana-Natalia, García-Domínguez, Irene, Alonso-Bellido, Isabel, González Ibáñez, Fernando, Picard, Katherine, Vázquez-Cabrera, Guillermo, Posada-Pérez, Mercedes, Vernoux, Nathalie, Tejera, Dario, Grabert, Kathleen, Cheray, Mathilde, González-Rodríguez, Patricia, Pérez-Villegas, Eva M., Martínez-Gallego, Irene, Lastra-Romero, Alejandro, Brodin, David, Avila-Cariño, Javier, Cao, Yang, Airavaara, Mikko, Uhlén, Per, Heneka, Michael T., Tremblay, Marie-Ève, Blomgren, Klas, Venero, Jose L., and Joseph, Bertrand

Abstract

Molecular diversity of microglia, the resident immune cells in the CNS, is reported. Whether microglial subsets characterized by the expression of specific proteins constitute subtypes with distinct functions has not been fully elucidated. Here we describe a microglial subtype expressing the enzyme arginase-1 (ARG1; that is, ARG1+ microglia) that is found predominantly in the basal forebrain and ventral striatum during early postnatal mouse development. ARG1+ microglia are enriched in phagocytic inclusions and exhibit a distinct molecular signature, including upregulation of genes such as Apoe, Clec7a, Igf1, Lgals3 and Mgl2, compared to ARG1– microglia. Microglial-specific knockdown of Arg1 results in deficient cholinergic innervation and impaired dendritic spine maturation in the hippocampus where cholinergic neurons project, which in turn results in impaired long-term potentiation and cognitive behavioral deficiencies in female mice. Our results expand on microglia diversity and provide insights into microglia subtype-specific functions.

Published
2023
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8. ARG1-expressing microglia show a distinct molecular signature and modulate postnatal development and function of the mouse brain

Author

Karolinska Institute, Swedish Research Council, Swedish Brain Foundation, Sigrid Juselius Foundation, Academy of Finland, Swedish Cultural Foundation, Swedish Cancer Society, Consejo Nacional de Ciencia y Tecnología (México), Fonds de la Recherche en Sante du Québec, Wenner-Gren Foundation, Ake Wiberg Foundation, Ministerio de Ciencia e Innovación (España), European Commission, Junta de Andalucía, Swedish Childhood Cancer Foundation, Canada Research Chairs, Stratoulias, Vassilis, Ruiz, Rocío, Kanatani, Shigeaki, Osman, Ahmed M., Keane, Lily, Armengol, José Ángel, Rodríguez-Moreno, Antonio, Murgoci, Adriana-Natalia, García-Domínguez, Irene, Alonso-Bellido, Isabel María, González Ibáñez, Fernando, Picard, Katherine, Vázquez Cabrera, Guillermo, Posada-Pérez, Mercedes, Vernoux, Nathalie, Tejera, Dario, Grabert, Kathleen, Cheray, Mathilde, González-Rodríguez, Patricia, Pérez-Villegas, Eva María, Martínez-Gallego, Irene, Lastra-Romero, Alejandro, Brodin, David, Ávila-Cariño, Javier, Cao, Yang, Airavaara, Mikko, Uhlén, Per, Heneka, Michael T., Tremblay, Marie-Ève, Blomgren, Klas, Venero, José L., Joseph, Bertrand, Karolinska Institute, Swedish Research Council, Swedish Brain Foundation, Sigrid Juselius Foundation, Academy of Finland, Swedish Cultural Foundation, Swedish Cancer Society, Consejo Nacional de Ciencia y Tecnología (México), Fonds de la Recherche en Sante du Québec, Wenner-Gren Foundation, Ake Wiberg Foundation, Ministerio de Ciencia e Innovación (España), European Commission, Junta de Andalucía, Swedish Childhood Cancer Foundation, Canada Research Chairs, Stratoulias, Vassilis, Ruiz, Rocío, Kanatani, Shigeaki, Osman, Ahmed M., Keane, Lily, Armengol, José Ángel, Rodríguez-Moreno, Antonio, Murgoci, Adriana-Natalia, García-Domínguez, Irene, Alonso-Bellido, Isabel María, González Ibáñez, Fernando, Picard, Katherine, Vázquez Cabrera, Guillermo, Posada-Pérez, Mercedes, Vernoux, Nathalie, Tejera, Dario, Grabert, Kathleen, Cheray, Mathilde, González-Rodríguez, Patricia, Pérez-Villegas, Eva María, Martínez-Gallego, Irene, Lastra-Romero, Alejandro, Brodin, David, Ávila-Cariño, Javier, Cao, Yang, Airavaara, Mikko, Uhlén, Per, Heneka, Michael T., Tremblay, Marie-Ève, Blomgren, Klas, Venero, José L., and Joseph, Bertrand

Abstract

Molecular diversity of microglia, the resident immune cells in the CNS, is reported. Whether microglial subsets characterized by the expression of specific proteins constitute subtypes with distinct functions has not been fully elucidated. Here we describe a microglial subtype expressing the enzyme arginase-1 (ARG1; that is, ARG1+ microglia) that is found predominantly in the basal forebrain and ventral striatum during early postnatal mouse development. ARG1+ microglia are enriched in phagocytic inclusions and exhibit a distinct molecular signature, including upregulation of genes such as Apoe, Clec7a, Igf1, Lgals3 and Mgl2, compared to ARG1- microglia. Microglial-specific knockdown of Arg1 results in deficient cholinergic innervation and impaired dendritic spine maturation in the hippocampus where cholinergic neurons project, which in turn results in impaired long-term potentiation and cognitive behavioral deficiencies in female mice. Our results expand on microglia diversity and provide insights into microglia subtype-specific functions.

Published
2023

11. Arg1+ microglia are critical for shaping cognition in female mice

Author

Stratoulias, Vassilis, primary, Ruiz, Rocío, additional, Kanatani, Shigeaki, additional, Osman, Ahmed M., additional, Armengol, Jose A., additional, Rodríguez-Moreno, Antonio, additional, Murgoci, Adriana-Natalia, additional, García-Domínguez, Irene, additional, Keane, Lily, additional, Vázquez-Cabrera, Guillermo, additional, Alonso-Bellido, Isabel, additional, Vernoux, Nathalie, additional, Tejera, Dario, additional, Grabert, Kathleen, additional, Cheray, Mathilde, additional, González-Rodríguez, Patricia, additional, Pérez-Villegas, Eva M., additional, Martinez-Gallego, Irene, additional, Brodin, David, additional, Avila-Cariño, Javier, additional, Airavaara, Mikko, additional, Uhlén, Per, additional, Heneka, Michael T., additional, Tremblay, Marie-Ève, additional, Blomgren, Klas, additional, Venero, Jose L., additional, and Joseph, Bertrand, additional

Published
2021
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14. Location of neonatal microglia drives small extracellular vesicles content and biological functions in vitro

Author

Murgoci, Adriana‐Natalia, primary, Duhamel, Marie, additional, Raffo‐Romero, Antonella, additional, Mallah, Khalil, additional, Aboulouard, Soulaimane, additional, Lefebvre, Christophe, additional, Kobeissy, Firas, additional, Fournier, Isabelle, additional, Zilkova, Monika, additional, Maderova, Denisa, additional, Cizek, Milan, additional, Cizkova, Dasa, additional, and Salzet, Michel, additional

Published
2020
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16. Paclitaxel Treatment and Proprotein Convertase 1/3 (PC1/3) Knockdown in Macrophages is a Promising Antiglioma Strategy as Revealed by Proteomics and Cytotoxicity Studies

Author

Duhamel, Marie, Rose, Mélanie, Rodet, Franck, Murgoci, Adriana Natalia, Zografidou, Lea, Régnier-Vigouroux, Anne, Abeele, Fabien Vanden, Kobeissy, Firas, Nataf, Serge, Pays, Laurent, Wisztorski, Maxence, Cizkova, Dasa, Fournier, Isabelle, Salzet, Michel, Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Banque de tissus et de cellules, Hôpital Edouard Herriot [CHU - HCL], Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Institut National de la Recherche Agronomique (INRA)

Subjects
Biochemistry & Molecular Biology, Calcium Signaling, Paclitaxel, Cell Survival, growth, [SDV]Life Sciences [q-bio], human gliomas, in-vitro, Exosomes, Cell Line, proteomics, glioma, Animals, proprotein convertase 1, breast-cancer, Secretome, taxol, Immunology, Brain Neoplasms, tumor-associated macrophages, Research, Antineoplastic Agents, Phytogenic, Coculture Techniques, peptide, quantification, Rats, macrophages, Cytokines, cells, nanoparticles, Cancer therapeutics
Abstract

International audience; High grade gliomas are the most common brain tumors in adult. These tumors are characterized by a high infiltration in microglial cells and macrophages. The immunosuppressive tumor environment is known to orient immune cells toward a pro-tumoral and anti-inflammatory phenotype. Therefore, the current challenge for cancer therapy is to find a way to reorient macrophages toward an antitumoral phenotype. Previously, we demonstrated that macrophages secreted antitumoral factors when they were invalidated for the proprotein converstase 1/3 (PC1/3) and treated with LPS. However, achieving an activation of macrophages via LPS/TLR4/Myd88-dependent pathway appears yet unfeasible in cancer patients. On the contrary, the antitumor drug Paclitaxel is also known to activate the TLR4 MyD88-dependent signaling pathway and mimics LPS action. Therefore, we evaluated if PC1/3 knock-down (KD) macrophages could be activated by Paclitaxel and efficient against glioma. We report here that such a treatment of PC1/3 KD macrophages drove to the overexpression of proteins mainly involved in cytoskeleton rearrangement. In support of this finding, we found that these cells exhibited a Ca2+ increase after Paclitaxel treatment. This is indicative of a possible depolymerization of microtubules and may therefore reflect an activation of inflammatory pathways in macrophages. In such a way, we found that PC1/3 KD macrophages displayed a repression of the anti-inflammatory pathway STAT3 and secreted more pro-inflammatory cytokines. Extracellular vesicles isolated from these PC1/3 KD cells inhibited glioma growth. Finally, the supernatant collected from the coculture between glioma cells and PC1/3 KD macrophages contained more antitumoral factors. These findings unravel the potential value of a new therapeutic strategy combining Paclitaxel and PC1/3 inhibition to switch macrophages toward an antitumoral immunophenotype.

Published
2018

17. Canine Bone Marrow-derived Mesenchymal Stem Cells: Genomics, Proteomics and Functional Analyses of Paracrine Factors

Author

Humenik, Filip, primary, Cizkova, Dasa, additional, Cikos, Stefan, additional, Luptakova, Lenka, additional, Madari, Aladar, additional, Mudronova, Dagmar, additional, Kuricova, Maria, additional, Farbakova, Jana, additional, Spirkova, Alexandra, additional, Petrovova, Eva, additional, Cente, Martin, additional, Mojzisova, Zuzana, additional, Aboulouard, Soulaimane, additional, Murgoci, Adriana-Natalia, additional, Fournier, Isabelle, additional, and Salzet, Michel, additional

Published
2019
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21. Shedding new light on spinal cord injury

Author

Salzet, Michel, primary, Quanico, Jusal, additional, Cizkova, Dasa, additional, Karnoub, Melodie Anne, additional, Laouby, Zarha, additional, Mériaux, Celine, additional, Murgoci, Adriana Natalia, additional, Rodet, Franck, additional, and Fournier, Isabelle, additional

Published
2018
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22. Correction: Cizkova, D., et al. Localized Intrathecal Delivery of Mesenchymal Stromal Cells Conditioned Media Improves Functional Recovery in A Rat Model of Contusive Spinal Cord Injury. Int. J. Mol. Sci. 2018, 19, 870

Author

Cizkova, Dasa, primary, Cubinkova, Veronika, additional, Smolek, Tomas, additional, Murgoci, Adriana-Natalia, additional, Danko, Jan, additional, Vdoviakova, Katarina, additional, Humenik, Filip, additional, Cizek, Milan, additional, Quanico, Jusal, additional, Fournier, Isabelle, additional, and Salzet, Michel, additional

Published
2018
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23. Localized Intrathecal Delivery of Mesenchymal Stromal Cells Conditioned Medium Improves Functional Recovery in a Rat Model of Spinal Cord Injury

Author

Cizkova, Dasa, primary, Cubinkova, Veronika, additional, Smolek, Tomas, additional, Murgoci, Adriana-Natalia, additional, Danko, Jan, additional, Vdoviakova, Katarina, additional, Humenik, Filip, additional, Cizek, Milan, additional, Quanico, Jusal, additional, Fournier, Isabelle, additional, and Salzet, Michel, additional

Published
2018
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26. Correction: Cizkova, D., et al. Localized Intrathecal Delivery of Mesenchymal Stromal Cells Conditioned Media Improves Functional Recovery in A Rat Model of Contusive Spinal Cord Injury. Int. J. Mol. Sci. 2018, 19, 870.

Author

Cubinkova, Veronika, Smolek, Tomas, Cizkova, Dasa, Murgoci, Adriana-Natalia, Danko, Jan, Vdoviakova, Katarina, Humenik, Filip, Quanico, Jusal, Fournier, Isabelle, Salzet, Michel, and Cizek, Milan

Subjects
STROMAL cells, SPINAL cord injuries
Abstract

A correction to the article "Localized Intrathecal Delivery of Mesenchymal Stromal Cells Conditioned Media Improves Functional Recovery in A Rat Model of Contusive Spinal Cord Injury" is presented.

Published
2018
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27. Paclitaxel Treatment and Proprotein Convertase 1/3 (PC1/3) Knockdown in Macrophages is a Promising Antiglioma Strategy as Revealed by Proteomics and Cytotoxicity Studies.

Author

Duhamel M, Rose M, Rodet F, Murgoci AN, Zografidou L, Régnier-Vigouroux A, Vanden Abeele F, Kobeissy F, Nataf S, Pays L, Wisztorski M, Cizkova D, Fournier I, and Salzet M

Subjects
Animals, Brain Neoplasms metabolism, Cell Line, Cell Survival drug effects, Coculture Techniques, Cytokines metabolism, Glioma metabolism, Macrophages drug effects, Macrophages metabolism, Proteomics, Rats, Antineoplastic Agents, Phytogenic pharmacology, Brain Neoplasms therapy, Glioma therapy, Paclitaxel pharmacology, Proprotein Convertase 1 genetics
Abstract

High grade gliomas are the most common brain tumors in adult. These tumors are characterized by a high infiltration in microglial cells and macrophages. The immunosuppressive tumor environment is known to orient immune cells toward a pro-tumoral and anti-inflammatory phenotype. Therefore, the current challenge for cancer therapy is to find a way to reorient macrophages toward an antitumoral phenotype. Previously, we demonstrated that macrophages secreted antitumoral factors when they were invalidated for the proprotein converstase 1/3 (PC1/3) and treated with LPS. However, achieving an activation of macrophages via LPS/TLR4/Myd88-dependent pathway appears yet unfeasible in cancer patients. On the contrary, the antitumor drug Paclitaxel is also known to activate the TLR4 MyD88-dependent signaling pathway and mimics LPS action. Therefore, we evaluated if PC1/3 knock-down (KD) macrophages could be activated by Paclitaxel and efficient against glioma. We report here that such a treatment of PC1/3 KD macrophages drove to the overexpression of proteins mainly involved in cytoskeleton rearrangement. In support of this finding, we found that these cells exhibited a Ca 2+ increase after Paclitaxel treatment. This is indicative of a possible depolymerization of microtubules and may therefore reflect an activation of inflammatory pathways in macrophages. In such a way, we found that PC1/3 KD macrophages displayed a repression of the anti-inflammatory pathway STAT3 and secreted more pro-inflammatory cytokines. Extracellular vesicles isolated from these PC1/3 KD cells inhibited glioma growth. Finally, the supernatant collected from the coculture between glioma cells and PC1/3 KD macrophages contained more antitumoral factors. These findings unravel the potential value of a new therapeutic strategy combining Paclitaxel and PC1/3 inhibition to switch macrophages toward an antitumoral immunophenotype., (© 2018 by The American Society for Biochemistry and Molecular Biology, Inc.)

Published
2018
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