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The effects of amending soil with gypsum and biochar on groundnut chlorophyll concentration, water use efficiency (WUE), biomass yield and selected soil properties were investigated under water stress. Gypsum (CaSO4.2H2O) was applied at 0 and 200 kg/ha, groundnut shell biochar at 1, 2 and 4% w/w of soil, and water at 100, 70 and 40% of daily plant water requirement (PWR) as main, sub and sub-sub plots, respectively, in a split-split-plot design. Biochar neutralized the acid soil, significantly raising soil pH from 5 to 7.15 and increasing cation exchange capacity by 75%. Biochar amended at 1 and 2%, increased groundnut dry matter yield by 28%. The optimum biochar application rate for dry matter yield was 1.4% w/w. Biochar application at 4% and irrigation at 40% of PWR reduced the WUE by 45 and 50%, respectively. Chlorophyll concentration index was highest at 40% of PWR. The results suggest that biochar has potential to raise soil pH, increase moisture retention and improve crop performance. Applying water at 100% PWR can increase groundnut dry matter yields, while higher gypsum application rates may be required to affect crop performance.

The paper reports findings from an evaluation of the effects of selected chemical and biological properties of soils under maize-cowpea cropping systems in Conservation Agriculture (CA) and their relationship to biological nitrogen fixation capabilities of cowpea. Soils from Kayowozi Agriculture Camp of Chipata District of Zambia where CA had been practiced for six years were evaluated. Cropping systems studied included conventional tillage (control), maize monocropping (sole maize), maize-cowpea intercrop, maize-cowpea rotation: maize phase and maize-cowpea: cowpea phase. Standard laboratory procedures were used to determine the changes in the selected soil properties as a result of these cropping sequences under CA. The study showed that maize- cowpea intercrop and rotation-maize phase under conservation agriculture could result in a significant increase in soil organic carbon, total nitrogen and exchangeable calcium after six years of practice. This increase can be associated with the amount and type of residue retained and the contribution of biologically fixed nitrogen from the cowpea. Having cowpea as the immediate previous crop in sequence can result in a depression of soil pH. Soil pH, total nitrogen, available phosphorus and exchangeable calcium in maize-cowpea cropping sequences can influence the amount of biologically fixed nitrogen. Changes in soil activity and microbial biomass might need more than six years to be apparent. The sequencing pattern of crops in a rotation, the choices and characteristics of crops, and the length of time of practice, all play an important role in determining interactions and processes leading to changes in soil properties and crop performance over time.

Soybean [Glycine max (L.) Merrill] is known for nitrogen fixation by rhizobia present in the soil with which it establishes an efficient symbiosis. In Zambia, current rhizobial inoculants used in soybeans production are based on non-indigenous strains; this creates a need to isolate local strains that can be used for the development of local inoculants for soybeans in Zambian soils. This paper reports the isolation and characterization of rhizobial isolates from virgin and cultivated soils of the three agro-ecological regions of Zambia. Rhizobia were isolated using the Trap Method and characterized using selected morphological and biochemical markers. A total of 61 isolates were isolated on Yeast Extract Mannitol (YEM) agar medium. Isolates varied in colony form, color, margin and texture. From the 61 isolates from the three regions, 87 % were circular, 8 % irregular and 5 % punctuate in form with 100 % convex elevation. The isolates had 88% entire, 10% undulate and 2 % lobate colony margins with different colors – 56 % cream, 24 % white, 11 % yellow, 5 % transparent and 3 % pink. Transparent colonies were peculiar to Region I and III while pink colonies were peculiar to Region III. All isolates produced mucous, were gram negative and rod shaped, a characteristic of rhizobial cells. None of the isolates could tolerate extremes of pH (4 and 9) in growth medium but grew well at pH 6.8. All isolates utilized glucose as a source of carbon. Based on the Bromothymol Blue (BTB) assay, 59 isolates were fast growing while two isolates from cultivated soils of region II were slow growing. The fast growing 59 isolates showed an acidic reaction changing the medium from green to yellow, while the others showed an alkaline reaction. Based on the results, the 59 fast-growers could be Ensifer fredii or/and Rhizobium tropici rather than Bradyrhizobium. However, further tests to confirm these findings using ketolactose, genetic characterization and inclusion of reference strains, are still needed and are being recommended here.

The use of inoculants is a critical strategy in legume production. In Zambia, inoculants are particularly used for the production of non-promiscuous genotypes of soya beans, but rarely for cowpeas and groundnuts. This study evaluated the response of soya beans, cowpeas and groundnuts to Biofix legume inoculants. Seeds were inoculated at the recommended or double the recommended rate at planting. Plants were grown under greenhouse conditions in a Completely Randomized Design for 7 weeks. Control, non-inoculated seeds were also planted and plants grown under the same conditions. At 7 weeks, nodule number and fresh weight per plant, nodule effectiveness (pinkness/redness), and above ground biomass were determined. Biologically fixed nitrogen was determined using the Nitrogen Difference Method. Nodule number and fresh weight per plant were higher at the recommended rate of Biofix application for soya beans and at both rates for groundnuts, while there was no effect on nodule fresh weight at either rate in cowpeas. All representative nodules assessed were effective. There was no significant benefit in inoculating seeds of the three legumes with Biofix on above ground biomass and biological nitrogen fixation. These results could suggest that possibly, the introduced strains though with a stronger nodulation competitiveness, were not as effective at fixing nitrogen as the indigenous strains in the soils in which soya beans, cowpeas and groundnuts have been repeatedly grown before. This could be an indication that sufficient and appropriate effective strains are already present in this soil. In general, the results suggest that to obtain the full benefits of biological nitrogen fixation, legume growers need to be provided with the correct inoculant, where required. Further work under field conditions is recommended to confirm these findings.