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Sidewall transfer lithography (STL) is demonstrated as a method for parallel fabrication of 2D nanostructured cellular solids in single-crystal silicon. The linear mechanical properties of four lattices (perfect and defected diamond; singly and doubly periodic honeycomb) with low effective Young’s moduli and effective Poisson’s ratio ranging from positive to negative are modelled using analytic theory and the matrix stiffness method with an emphasis on boundary effects. The lattices are fabricated with a minimum feature size of 100 nm and an aspect ratio of 40:1 using single- and double-level STL and deep reactive ion etching of bonded silicon-on-insulator. Nanoelectromechanical systems (NEMS) containing cellular materials are used to demonstrate stretching, bending and brittle fracture. Predicted edge effects are observed, theoretical values of Poisson’s ratio are verified and failure patterns are described. [ABSTRACT FROM AUTHOR]

Antimony sulfoselenide (Sb 2 (S,Se) 3 ) is a promising sunlight absorber material for solar energy conversion in photovoltaic (PV) cells and photoelectrochemical (PEC) photoelectrodes due to its excellent photoelectric properties. However, the obtained thin-film and back contact properties significantly influence the PEC performance of photocathodes, causing severe bulk recombination, carrier transport loss, and deteriorating half-cell solar-to-hydrogen (HC-STH) efficiency. This study introduces an intriguing dual back interface engineering strategy for Sb 2 (S,Se) 3 photocathodes by incorporating an intermediate MoO 2 layer and a secondary carrier transport channel of Au to strengthen charge carrier dynamics. The synergistic assembly of these dual back interface layers improves the growth kinetics and achieves the optimal orientation of Sb 2 (S,Se) 3 thin films by increasing substrate wettability. Moreover, by shortening the back contact barrier height and passivating defect-assisted recombinations, these dual back underlayers simultaneously enhance carrier transport and separation efficiencies. As a result, the photocurrent density of the champion Sb 2 (S,Se) 3 photocathode increases from 5.89 to 32.60 mA cm -2 , and the HC-STH conversion efficiency improves significantly from 0.30% to 3.58%, representing the highest value for Sb 2 (S,Se) 3 -based photocathodes. This work highlights the effectiveness of dual back interface engineering in promoting the PEC performance of chalcogenide photocathodes for solar hydrogen evolution applications., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)

Sparassis latifolia often referred to as Cauliflower mushroom possess both medicinal and edibility values. In this research work, first time laccase purification and dye colourisation efficacy of Sparassis latifolia 's purified laccase were assessed. Optimal laccase potential was noted after 12 th day of incubation with 4 pH of medium at 45 °C. As supplementary nutritional source, sucrose and ammonium sulphate were unveiled as most effective synthetic carbon and nitrogen sources while wheat straw found as proficient lignocellulosic biomass for enhanced laccase production. Following optimisation of laccase production parameters, laccase was purified to 14.2 fold by ammonium sulphate precipitation, dialysis and anion exchange chromatography. Laccase molecular weight determined through SDS PAGE was found to be of 65 KDa. This purified laccase was tested for its ability to decolourise the Congo red at definite pH and temperature. It was found that this fungus has capability to decolourise up to 98.6% of Congo-red dye.

Background: X-chromosomal short tandem repeats (X-STRs) are crucial in forensic applications, particularly in complex kinship cases, and play an important role in population genetics. However, there is limited data on X-STR variation in Pakistani populations, especially among ethnic groups like Kashmiri and Punjabi. Methodology: This study investigates the forensic and genetic properties of 12 X-STRs from the Investigator Argus X-12 Kit (QIAGEN, Hilden, Germany) in 125 families (75 Kashmiri, 50 Punjabi) from Azad Jammu and Kashmir and Punjab, Pakistan. Results: In both populations, a total of 222 alleles were identified across the 12 X-STR loci (Punjabi 171 alleles, Kashmiri 161 alleles), with allele frequencies ranging from 0.0056 to 0.3033. DXS10148 was the most polymorphic locus with 28 alleles, while DXS7132 was the least polymorphic with 9 alleles. Most loci were in linkage equilibrium, except for the DXS10135/DXS10148 pair in males, with no loci exhibiting significant linkage disequilibrium in females. The combined power of discrimination was 0.999 999 9977 for Kashmiri males, 0.999 999 999 999 9746 for Kashmiri females, and 0.999 999 999 999 9781 for Punjabi females. In Kashmiri males, 34, 31, 28, and 32 haplotypes were observed across the four linkage groups (LG1, LG2, LG3, and LG4), though these groups did not form stable haplotypes, as indicated by Linkage Equilibrium within and significant Linkage Disequilibrium between groups. Conclusions: Genetic structure analysis using Principal Component Analysis and STRUCTURE revealed distinct clustering patterns for the Kashmiri and Punjabi populations, indicating unique genetic backgrounds and ancestry influences, particularly distinguishing them from East Asian populations. This study provides a comprehensive analysis of X-STR variation in Punjabi and Kashmiri populations, offering valuable insights for forensic and population genetic studies.

The early prediction of ocular disease is certainly an obligatory concern in the domain of ophthalmic medicine. Although modern scientific discoveries have shown the potential to treat eye diseases by using artificial intelligence (AI) and machine learning, explainable AI remains a crucial challenge confronting this area of research. Although some traditional methods put in significant effort, they cannot accurately predict the proper ocular diseases. However, incorporating AI into diagnosing eye diseases in healthcare complicates the situation as the decision-making process of AI demonstrates complexity, which is a significant concern, especially in major sectors like ocular disease prediction. The lack of transparency in the AI models may hinder the confidence and trust of the doctors and the patients, as well as their perception of the AI and its abilities. Accordingly, explainable AI is significant in ensuring trust in the technology, enhancing clinical decision-making ability, and deploying ocular disease detection. This research proposed an efficient transfer learning model for eye disease prediction to transform smart vision potential in the healthcare sector and meet conventional approaches' challenges while integrating explainable artificial intelligence (XAI). The integration of XAI in the proposed model ensures the transparency of the decision-making process through the comprehensive provision of rationale. This proposed model provides promising results with 95.74% accuracy and explains the transformative potential of XAI in advancing ocular healthcare. This significant milestone underscores the effectiveness of the proposed model in accurately determining various types of ocular disease. It is clearly shown that the proposed model is performing better than the previously published methods.

Objective: The need for safe and reliable mechanical circulatory support (MCS) for smaller children with severe heart failure (HF) is well defined. More specifically, in pediatric patients with advanced congenital HF, there is no implantable total artificial heart (TAH) device available for small patients. Herein, we report the development of the infant continuous-flow total artificial heart (I-CFTAH), a fully implantable in infants and newborns., Methods: After extensive engineering analysis, we performed an unprecedented effort: reducing the I-CFTAH's displacement volume to be 14% of the adult CFTAH pump while simultaneously decreasing pump diameter (6.2 cm to 2.6 cm) and axial length (9.8 cm to 4.8 cm). Facilitated by these proportional reductions, for the first time, a durable total artificial heart device was successfully fit in the chest of infants and newborns (height of ≥50 cm)., Results: The functional I-CFTAH prototype demonstrated capability to support stable hemodynamics and desired device performance. The pump flow range (0.5-1.5 L/min) was confirmed in a mock circulatory testing loop. Within the tested flow range, the I-CFTAH can support small patients that could benefit from the intended cardiac output., Conclusions: This successful effort demonstrated the feasibility of the miniature continuous-flow total artificial heart, intended for very small patient populations. I-CFTAH showed stable hemodynamics and could, therefore, become one of the few therapeutic options as a bridge to transplantation, aiming to enhance both the quality and duration of life for pediatric patients with advanced HF., Competing Interests: J.H.K., B.K., and K.F. and are coinventors of the I-CFTAH. All other authors reported no conflicts of interest. The Journal policy requires editors and reviewers to disclose conflicts of interest and to decline handling or reviewing manuscripts for which they may have a conflict of interest. The editors and reviewers of this article have no conflicts of interest., (© 2024 The Author(s).)

Competing Interests: The authors reported no conflicts of interest. The Journal policy requires editors and reviewers to disclose conflicts of interest and to decline handling or reviewing manuscripts for which they may have a conflict of interest. The editors and reviewers of this article have no conflicts of interest.

Heat stress poses a significant environmental challenge that profoundly impacts wheat productivity. It disrupts vital physiological processes such as photosynthesis, by impeding the functionality of the photosynthetic apparatus and compromising plasma membrane stability, thereby detrimentally affecting grain development in wheat. The scarcity of identified marker trait associations pertinent to thermotolerance presents a formidable obstacle in the development of marker-assisted selection strategies against heat stress. To address this, wheat accessions were systematically exposed to both normal and heat stress conditions and phenotypic data were collected on physiological traits including proline content, canopy temperature depression, cell membrane injury, photosynthetic rate, transpiration rate (at vegetative and reproductive stage and 'stay-green'. Principal component analysis elucidated the most significant contributors being proline content, transpiration rate, and canopy temperature depression, which exhibited a synergistic relationship with grain yield. Remarkably, cluster analysis delineated the wheat accessions into four discrete groups based on physiological attributes. Moreover, to explore the relationship between physiological traits and DNA markers, 158 wheat accessions were genotyped with 186 SSRs. Allelic frequency and polymorphic information content value were found to be highest on genome A (4.94 and 0.688), chromosome 1A (5.00 and 0.712), and marker Xgwm44 (13.0 and 0.916). Population structure, principal coordinate analysis and cluster analysis also partitioned the wheat accessions into four subpopulations based on genotypic data, highlighting their genetic homogeneity. Population diversity and presence of linkage disequilibrium established the suitability of population for association mapping. Additionally, linkage disequilibrium decay was most pronounced within a 15-20 cM region on chromosome 1A. Association mapping revealed highly significant marker trait associations at Bonferroni correction P < 0.00027. Markers Xwmc418 (located on chromosome 3D) and Xgwm233 (chromosome 7A) demonstrated associations with transpiration rate, while marker Xgwm494 (chromosome 3A) exhibited an association with photosynthetic rates at both vegetative and reproductive stages under heat stress conditions. Additionally, markers Xwmc201 (chromosome 6A) and Xcfa2129 (chromosome 1A) displayed robust associations with canopy temperature depression, while markers Xbarc163 (chromosome 4B) and Xbarc49 (chromosome 5A) were strongly associated with cell membrane injury at both stages. Notably, marker Xbarc49 (chromosome 5A) exhibited a significant association with the 'stay-green' trait under heat stress conditions. These results offers the potential utility in marker-assisted selection, gene pyramiding and genomic selection models to predict performance of wheat accession under heat stress conditions., (© 2024. The Author(s).)

Water scarcity and droughts are among the most challenging issues worldwide, particularly in arid and semi-arid regions like Saudi Arabia. Date palm (Phoenix dactylifera L.), a major crop in Saudi Arabia, is being significantly affected by water scarcity, soil salinity, and desertification. Alternative water sources are needed to conserve freshwater resources and increase date palm production in Saudi Arabia. On the other hand, Saudi Arabia has a significant number of aquaculture farms that generate substantial amounts of wastewater, which can be utilized as an alternative source of irrigation. Therefore, this study aimed to assess the potential of aquaculture wastewater as an alternative irrigation source for date palm orchards. Aquaculture wastewater was collected from 12 different farms (Al-Kharj, Al-Muzahmiya, and Al-Qassim regions, Saudi Arabia) and its quality was analyzed. The impacts of aquaculture wastewater irrigation on soil quality, nutrient availability, nutrient status of date palm trees, and dates fruit quality were assessed in comparison to source water (freshwater) irrigation at Al-Kharj, Al-Muzahmiya, and Al-Qassim regions. The water quality analyses showed higher salinity (EC = 3.31 dSm -1 ) in farm Q3, while all other farms demonstrated no salinity, sodicity, or alkalinity hazards. Moreover, the aquaculture wastewater irrigation increased soil available P, K, NO 3 - -N, and NH 4 + -N by 49.31%, 21.11%, 33.62%, and 52.31%, respectively, compared to source water irrigation. On average, date palm fruit weight, length, and moisture contents increased by 26%, 23%, and 43% under aquaculture wastewater irrigation compared to source water irrigation. Further, P, K, Fe, Cu, and Zn contents in date palm leaf were increased by 19.35%, 34.17%, 37.36%, 38.24%, and 45.29%, respectively, under aquaculture wastewater irrigation compared to source water irrigation. Overall, aquaculture wastewater irrigation significantly enhanced date palm plant growth, date palm fruit quality, and soil available nutrients compared to freshwater irrigation. It was concluded that aquaculture wastewater can be used as an effective irrigation source for date palm farms as it enhances soil nutrient availability, date palm growth, and date fruit yield and quality. The findings of this study suggest that aquaculture wastewater could be a viable alternative for conserving freshwater resources and increase date palm production in Saudi Arabia., (© 2024. The Author(s).)

Wearing face masks is the best way to stop the spread of respiratory infections. However, if masks are not sterilized, changing them too frequently can actually increase the risk of cross-contamination. Herein, the construction of an antipathogen photocatalytic mask with carbon vacancy-modified carbon nitride nanosheets (g-C 3 N 4 -V C Ns) coated on the non-woven fabrics of the out layer of the mask, offering effective and long-term protection against damaging pathogens when exposed to light is reported. The introduced carbon vacancies are found capable of creating energy-disordered sites and inducing energetic electric force to overcome the Coulomb interactions between electron-hole pairs, thus promoting the electron-hole separation to achieve a high generation of reactive oxygen species (ROS). Thanks to its high activity in generating ROS upon exposure to light, the as-prepared photocatalytic mask shows high pathogen sterilization performance. This, in turn, prolongs the mask's protective lifetime, decreases the need for regular replacement, and decreases medical waste production. The work demonstrated here opens new viewpoints in designing pathogens biocidal protective devices for health protection, offering significant promise in specific environment self-protection., (© 2024 The Author(s). Advanced Science published by Wiley‐VCH GmbH.)

The prevalence of antibiotic-resistant pathogenic bacteria poses a significant threat to public health and ranks among the principal causes of morbidity and mortality worldwide. Antimicrobial photodynamic therapy is an emerging therapeutic technique that has excellent potential to embark upon antibiotic resistance problems. The efficacy of this therapy hinges on the careful selection of suitable photosensitizers (PSs). Transition metal complexes, such as Ruthenium (Ru) and Iridium (Ir), are highly suitable for use as PSs because of their surface plasmonic resonance, crystal structure, optical characteristics, and photonics. These metals belong to the platinum family and exhibit similar chemical behavior due to their partially filled d-shells. Ruthenium and Iridium-based complexes generate reactive oxygen species (ROS), which interact with proteins and DNA to induce cell death. As photodynamic therapeutic agents, these complexes have been widely studied for their efficacy against cancer cells, but their potential for antibacterial activity remains largely unexplored. Our study focuses on exploring the antibacterial photodynamic effect of Ruthenium and Iridium-based complexes against both Gram-positive and Gram-negative bacteria. We aim to provide a comprehensive overview of various types of research in this area, including the structures, synthesis methods, and antibacterial photodynamic applications of these complexes. Our findings will provide valuable insights into the design, development, and modification of PSs to enhance their photodynamic therapeutic effect on bacteria, along with a clear understanding of their mechanism of action., Competing Interests: Declaration of competing interest We declare that we have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Intercropping, a widely adopted agricultural practice worldwide, aims to increase crop yield, enhance plant nutrient uptake, and optimize the utilization of natural resources, contributing to sustainable farming practices on a global scale. However, the underlying changes in soil physio-chemical characteristics and enzymatic activities, which contribute to crop yield and nutrient uptake in the intercropping systems are largely unknown. Consequently, a two-year (2021-2022) field experiment was conducted on the maize/soybean intercropping practices with/without nitrogen (N) fertilization (i.e., N 0 ; 0 N kg ha -1 and N 1 ; 225 N kg ha -1 for maize and 100 N kg ha -1 for soybean ) to know whether such cropping system can improve the nutrients uptake and crop yields, soil physio-chemical characteristics, and soil enzymes, which ultimately results in enhanced crop yield. The results revealed that maize intercropping treatments (i.e., N 0 MI and N 1 MI) had higher crop yield, biomass dry matter, and 1000-grain weight of maize than mono-cropping treatments (i.e., N 0 MM, and N 1 MM). Nonetheless, these parameters were optimized in N 1 MI treatments in both years. For instance, N 1 MI produced the maximum grain yield (10,105 and 11,705 kg ha -1 ), biomass dry matter (13,893 and 14,093 kg ha -1 ), and 1000-grain weight (420 and 449 g) of maize in the year 2021 and 2022, respectively. Conversely, soybean intercropping treatments (i.e., N 0 SI and N 1 SI) reduced such yield parameters for soybean. Also, the land equivalent ratio (LER) and land equivalent ratio for N fertilization (LER N ) values were always greater than 1, showing the intercropping system's benefits in terms of yield and improved resource usage. Moreover, maize intercropping treatments (i.e., N 0 MI and N 1 MI) and soybean intercropping treatments (i.e., N 0 SI and N 1 SI) significantly (p < 0.05) enhanced the nutrient uptake (i.e., N, P, K, Ca, Fe, and Zn) of maize and soybean, however, these nutrients uptakes were more prominent in N 1 MI and N 1 SI treatments of maize and soybean, respectively in both years (2021 and 2022) compared with their mono-cropping treatments. Similarly, maize-soybean intercropping treatments (i.e., N 0 MSI and N 1 MSI) significantly (p < 0.05) improved the soil-based N, P, K, NH 4 , NO 3 , and soil organic matter, but, reduced the soil pH. Such maize-soybean intercropping treatments also improved the soil enzymatic activities such as protease (PT), sucrose (SC), acid phosphatase (AP), urease (UE), and catalase (CT) activities. This indicates that maize-soybean intercropping could potentially contribute to higher and better crop yield, enhanced plant nutrient uptake, improved soil nutrient pool, physio-chemical characteristics, and related soil enzymatic activities. Thus, preferring intercropping to mono-cropping could be a preferable choice for ecologically viable agricultural development., (© 2024. The Author(s).)

An efficient perovskite-based heterogeneous catalyst is highly desired to activate peroxymonosulfate (PMS) for removing organic pollutants in water. A high surface area PMS-activator was fabricated by loading LaCoO 3 on SBA-15 to degrade atrazine (ATR) in water. The LaCoO 3 /SBA-15 depicted better textural properties and higher catalytic activity than LaCoO 3 . In 6.0 min, atrazine (ATZ) degradation in the selected LaCoO 3 /SBA-15/PMS system, LaCoO 3 , adsorption by LaCoO 3 /SBA-15, sole PMS processes reached approximately 100%, 55.15%, 12.80%, and 16.65 % respectively. Furthermore, 0.04 mg L -1 Co was leached from LaCoO 3 /SBA-15 during PMS activation by LaCoO 3 /SBA-15. The LaCoO 3 /SBA-15 showed stable catalytic activity after reuse. The use of radical scavengers and electron paramagnetic resonance spectroscopy (EPR) demonstrated that ROS such as 1 O 2 , O 2 •- , • OH, and SO 4 •- were generated by PMS activated by LaCoO 3 /SBA-15 owing to redox reactions [Co 2+ /Co 3+ , and O 2- /O 2 ]. EPR, XPS, ATR-FTIR, EIS, LSV, and chronoamperometric measurements were used to explain the catalytic mechanism for PMS activation. Excellent atrazine degradation was due to high surface area, porous nature, diffusion-friendly structure, and ROS. Our investigation proposes that perovskites with different A and B metals and modified perovskites can be loaded on high surface area materials to activate PMS into ROS., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Although the conversion of O 2 and H 2 O to H 2 O 2 over graphite carbon nitride (g-C 3 N 4 ) has been realized by means of the photocatalytic process, the catalytic activity of pristine g-C 3 N 4 is still restricted by the rapid charge recombination and inadequate exposure of the active site. In this work, we propose a straightforward strategy to solve these limitations by decreasing the thickness and improving the crystallinity of g-C 3 N 4 , resulting in the preparation of few-layered crystalline carbon nitride (FL-CCN). Benefiting from the minimal thickness and highly ordered in-plane triangular cavities within the structure, FL-CCN processes an extended π-conjugated system with a reduced charge transfer resistance and expanded specific surface area. These features accelerate the efficiency of photogenerated charge separation in FL-CCN and contribute to explore of its surface active sites. Consequently, FL-CCN exhibits a significantly improved H 2 O 2 evolution rate (63.95 μmol g -1 h -1 ), which is 7.8 times higher than that of pristine g-C 3 N 4 (8.15 μmol g -1 h -1 ), during the photocatalytic conversion of O 2 and H 2 O. This systematic investigation offers valuable insights into the mechanism of photocatalytic H 2 O 2 generation and the development of efficient catalysts.

Ralstonia solanacearum is a devastating soilborne pathogen which poses significant yield and economic losses to tobacco production globally. The impact of R. solanacearum on rhizosphere bacteriome and soil physicochemical characteristics in resistant and susceptible tobacco cultivars is poorly understood. This study aims to determine the effect of R. solanacearum on soil physicochemical parameters and rhizosphere bacteriome of resistant (K326) and susceptible (Hongda) tobacco cultivars at various growth stages. Results demonstrated that the contents of available potassium and phosphorus, as well as soil pH were significantly increased in K326 soils (CK and T2) compared with Hongda (T1) after 21, 42, and 63 days post-inoculation (dpi) of R. solanacearum except for available nitrogen which showed an opposite trend. The qPCR results showed a significant decrease in R. solanacearum population in rhizosphere of K326 (T2) compared to the Hongda (T1) at 21 and 63 dpi than that after 42 dpi. The rhizosphere bacteriome analysis through 16S rRNA amplicon sequencing revealed that rhizosphere bacterial community composition was significantly different between two tobacco cultivars (Hongda and K326) and this effect was more prominent after 63 dpi (93 days after post-transplantation), suggesting that each cultivar recruits a unique set of bacterial communities. There was no obvious difference observed in the rhizosphere bacteriome of CK (K326) and T2 (K326), which might be attributed to the same genetic makeup and inherent resistance of K326 to bacterial wilt infection. Analysis of co-occurrence networks revealed that the microbial network in T1 (Hongda) was more complex than those in T2 (K326) and CK (K326), while the networks in CK and T2 were almost identical. The present research highlights the time-course relationship between environmental factors and rhizosphere bacteriome of tobacco cultivars showing different levels of resistance against R. solanacearum. Conclusively, studying the plant-soil-microbe interaction system in susceptible and resistant tobacco cultivars may enable us to develop effective integrated disease control plans for the healthy production of tobacco crops., Competing Interests: Declaration of Competing Interest There is no financial and non-financial conflict of interest among authors., (Copyright © 2024 Elsevier GmbH. All rights reserved.)

The wide range of applications of nanoparticles (NPs) in various industries have led to serious consequences in terms of teratogenic toxicity. The aim of current work was to evaluate the teratogenic effects of copper oxide (CuO) nanoparticles in albino mice.In this experimental study, after mating, inseminated 40 female mice were divided randomly into 4 pools (1 control and 3 experimental), ten each. Doses were administered intravenously (We followed the protocol by Yaqub et al. (2018), intravenous application is faster route as compared to oral dosage)to all the experimental groups on the 6th day of gestation (GD), dose concentrations were 200, 133.3 and 100 mg/kg body weights respectively.The doses were prepared in sequence (1/2, 1/3, 1/4 0f LD50) according to already published work. The effects of CuO-NPs show linear relationship with the above sequence. The control group was administered only with distilled water.The gravid females were sacrificed through cervical disruption at the 18th day of gestation, fetuses were removed and divided into four sets (pools) for morphometric, morphological and histological studies. Data were subjected to statistical analysis by using Tukey's test in light of ANOVA at p < 0.05 level of significance. Findings of the present study showed that CuO-NPs various concentrations affect developmental abnormalities i.e.runt embryos, resorbed uteri, exencephaly, hygroma, macroglossia, micromelia, open eye, omphalocoel, scoliosis, kyphosis and kinked tail. It is concluded that exposure to CuO-NPs may potentially lead to the developmental deformities in mice., Competing Interests: Declaration of competing interest The authors declare that they have no competing interests., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

In this study, biochar (BC) and hydrochar (HC) composites were synthesized with natural kaolinite clay and their properties, stability, carbon (C) sequestration potential, polycyclic aromatic hydrocarbons (PAHs) toxicity, and impacts on maize germination and growth were explored. Conocarpus waste was pretreated with 0%, 10%, and 20% kaolinite and pyrolyzed to produce BCs (BC, BCK10, and BCK20, respectively), while hydrothermalized to produce HCs (HC, HCK10, and HCK20, respectively). The synthesized materials were characterized using X-ray diffraction, scanning electron microscope analyses, Fourier transform infrared, thermogravimetric analysis, surface area, proximate analyses, and chemical analysis to investigate the distinction in physiochemical and structural characteristics. The BCs showed higher C contents (85.73-92.50%) as compared to HCs (58.81-61.11%). The BCs demonstrated a higher thermal stability, aromaticity, and C sequestration potential than HCs. Kaolinite enriched-BCs showed the highest cation exchange capacity than pristine BC (34.97% higher in BCK10 and 38.04% higher in BCK20 than pristine BC), while surface area was the highest in kaolinite composited HCs (202.8% higher in HCK10 and 190.2% higher in HCK20 than pristine HC). The recalcitrance index (R 50 ) speculated a higher recalcitrance for BC, BCK10, and BCK20 (R 50  > 0.7), minimal degradability for HCK10 and HCK20 (0.5 < R 50  < 0.7), and higher degradability for biomass and HC (R 50  < 0.5). Overall, increasing the kaolinite enrichment percentage significantly enhanced the thermal stability and C sequestration potential of charred materials, which may be attributed to changes in the structural arrangements. The ∑ total PAHs concentration in the synthesized materials were below the USEPA's suggested limits, indicating their safe use as soil amendments. Germination indices reflected positive impacts of synthesized charred materials on maize germination and growth. Therefore, we propose that kaolinite-composited BCs and HCs could be considered as efficient and cost-effective soil amendments for improving plant growth., (© 2024. The Author(s).)

Chymosin, an aspartic protease present in the stomachs of young ruminants like cows (bovine), causes milk coagulation and cheese production through the breakdown of κ-casein peptide bonds at the Met105-Phe106 site. Bovine chymosin is first synthesized as a pre-prochymosin that is cleaved to produce the mature chymosin protein. Despite significant strides in research, our understanding of this crucial enzyme remains incomplete. The purpose of this work was to perform in silico evolutionary and functional analysis and to gain unique insights into the structure of this protein. For this, the sequence of Bos taurus chymosin from UniProt database was subjected to various bioinformatics analyses. We found that bovine chymosin is a low molecular weight and hydrophilic protein that has homologs in other Bovidae species. Two active sites of aspartic peptidases, along with a functional domain, were identified. Gene Ontology analysis further confirmed chymosin's involvement in proteolysis and aspartic endopeptidase activity. Potential disordered residues and post-translational modification sites were also uncovered. It was revealed that the secondary structure of bovine chymosin is comprised of beta strands (44.27%), coils (43.65%), and alpha helices (12.07%). A highly optimized 3D structure was also obtained. Moreover, crucial protein-protein interactions were unveiled. Altogether, these findings provide valuable insights that could guide future research on bovine chymosin and its biological roles., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2023 The Authors.)

Background: Complex patients requiring operations on the left ventricular outflow tract, aortic valve, or thoracic aorta after previous repair of aortopathy constitute a challenging group, with limited information guiding decision-making. We aimed to use our institutional experience to highlight management challenges and describe surgical pearls to address them., Methods: Forty-one complex patients with surgery on the left ventricular outflow tract, aortic valve, or aorta at Cleveland Clinic Children's between 2016 and 2021 following previous repair of aortic pathology were retrospectively reviewed. Patients with known connective tissue disease or single ventricle circulation were excluded., Results: Median age at index procedure was 23 years (range 0.25-48) with median of 2 prior sternotomies. Previous aortic operations included subvalvular (n = 9), valvular (n = 6), supravalvular (n = 13), and multi-level surgeries (n = 13). Four deaths occurred in median follow-up of 2.5 years. Mean left ventricular outflow tract gradients improved significantly for patients with obstruction (34.9 ± 17.5 mmHg versus 12.6 ± 6.0 mmHg; p < 0.001). Technical pearls include the following: 1) liberal use of anterior aortoventriculoplasty with valve replacement; 2) primarily anterior aortoventriculoplasty following the subpulmonary conus in contrast to more vertical incision for post-arterial switch operation patients; 3) pre-operative imaging of mediastinum and peripheral vasculature for cannulation and sternal re-entry; and 4) proactive use of multi-site peripheral cannulation., Conclusions: Operation to address the left ventricular outflow tract, aortic valve, or aorta following prior congenital aortic repair can be accomplished with excellent outcomes despite high complexity. These procedures commonly include multiple components, including concomitant valve interventions. Cannulation strategies and anterior aortoventriculoplasty in specific patients require modifications.

Background: Relationships between pulmonary hypertension (PHTN), mitral valve intervention (MVI), and death among patients with Shone's syndrome (SS) are unclear. Our recent data suggested temporal progression of MV disease and early survival benefit with MVI at initial operation. In this expanded cohort, we characterized trajectory and impact of PHTN on survival, and its relationship to MVI(s)., Methods: Record review of 219 SS patients undergoing operation at our quaternary center between 1956-2022, 111 of whom were pediatric (< age 18 yrs.). Patients were determined to have PHTN using a time-defined hierarchical algorithm consisting of cardiac catheterization data, echocardiographic parameters, pulmonary vasodilator therapy, and record-verified PHTN diagnosis. Analysis includes risk-adjusted predicted survival and freedom from PHTN curves stratified by MVI timing., Results: Among 219 patients, 92 (43%) had mitral valve stenosis on presentation, with mean gradients of 3.5 (IQR 4.7). Sixty patients had at least one MVI (15 (6%) and 8 (3%) had 3 or more). Median follow-up was 19 years (IQR 29) with 18 (8%) deaths. Ninety-three (42%) patients developed PHTN, with 50% and 25% freedom from PHTN at 37 and 51 years from presentation. PHTN was strongly associated with death (p <0.001). In unadjusted analyses, MVI, even if successive, delayed the onset of PHTN at 10 years and improved survival (p<0.001). However, this protective effect was lost after risk-adjustment., Conclusions: PHTN development in SS is progressive and mirrors the survival curve, suggesting it is a major mortality risk-factor. Salutary effects of early and/or successive MVI are reduced after risk-adjustment., (Copyright © 2023. Published by Elsevier Inc.)

Microplastics (MPs) are emerging environmental pollutants worldwide, posing potential health risks. Moreover, MPs may act as vectors for other contaminants and affect their fate, transport, and deposition in the environment. Therefore, efficient and economical techniques are needed for the removal of contemporary MPs and contaminants from the environment. The present research study investigated the sorption of phosphorus (P) and ammonium (NH 4 + ) onto date palm waste-derived biochar (BC) from an aqueous solution in the presence of polyamide (PA) and polyethylene (PE) MPs. The BC was prepared at 600 °C, characterized for physio-chemical properties, and applied for P and NH 4 + removal via isotherm and kinetic sorption trials. The results of the sorption trials demonstrated the highest removal of NH 4 + and P was obtained at neutral pH 7. The highest P sorption (93.23 mg g -1 ) by BC was recorded in the presence of PA, while the highest NH 4 + sorption (103.76 mg g -1 ) was found with co-occurring PE in an aqueous solution. Sorption isotherm and kinetics models revealed that P and NH 4 + removal by MP-amended BC followed chemisorption, electrostatic interaction, precipitation, diffusion, and ion exchange mechanisms. Overall, co-existing PA enhanced the removal of P and NH 4 + by 66% and 7.7%, respectively, while co-existing PE increased the removal of P and NH 4 + by 55% and 30%, respectively, through the tested BC. Our findings suggested that converting date palm waste into BC could be used as a competent and economical approach to removing P and NH 4 + from contaminated water. Furthermore, microplastics such as PE and PA could assist in the removal of P and NH 4 + from contaminated water using BC.

Extensive production and utilization of plastic products have resulted in the generation of microplastics (MPs), subsequently polluting the environment. The efficiency of biochars (BCs) derived from jujube (Ziziphus jujube L.) biomass (300 °C and 700 °C) for nylon (NYL) and polyethylene (PE) removal from contaminated water was explored in fixed-bed column trials. The optimum pH for the removal of both MPs was found 7. Both of the produced biochars demonstrated >99% removal of the MPs, while the sand filter exhibited a maximum of 78% removal of MPs. BC produced at 700 °C (BC700) showed 33-fold higher MPs retention, while BC produced at 300 °C (BC300) exhibited 20-fold higher retention, as compared to sand filters, indicating the higher efficiency of BC produced at higher pyrolysis temperature. Entrapment into the pores, entanglement with flaky structures of the BCs, and electrostatics interactions were the major mechanism for MPs retention in BCs. The efficiency of MPs-amended BCs was further explored for the removal of Pb(II) and Cd(II) in fixed-bed column trials. BC700 amended with PE and NYL exhibited the highest 50% breakthrough time (2114.23 and 2024.61 min, respectively, for Pb(II) removal and 2107.92 and 1965.19 min, respectively, for Cd(II) removal), as compared to sand filters (38.07 and 60.49 min for Pb(II) and Cd(II) removal, respectively). Thomas model predicted highest adsorption capacity was exhibited by BC700 amended with PE (584.34 and 552.80 mg g -1 , for Pb(II) and Cd(II) removal, respectively), followed by BC700 amended with NYL (557.65 and 210.59 mg g -1 for Pb(II) and Cd(II) removal, respectively). Therefore, jujube waste-derived BCs could be used as efficient adsorbents to remove PE and NYL from contaminated water, while MPs-loaded BCs can further be utilized for higher adsorption of Pb(II) and Cd(II) from contaminated aqueous media. These findings suggest that BC could be used as an efficient adsorbent to remove the co-existing MPs-metals ions from the environment on a sustainable basis., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

This paper aims to analyze the link between environmental degradation and institutional quality and the price of oil moderated by economic complexity and the underground economy. We use quantile regressions with annual panel data for 15 countries in the Middle East and North Africa during 1995-2021. The findings indicate that institutional quality, economic complexity, and output positively and heterogeneously impact environmental degradation. However, the square of production has a negative impact, confirming an inverted U relationship between production and environmental degradation. Likewise, we find that the price of oil and the underground economy have a negative and heterogeneous impact on environmental degradation. Based on our results, a potential recommendation for policymakers is that the institutional framework of Middle Eastern and North African countries should be accompanied by a more significant concern for the environment instead of prioritizing extractive growth that is detrimental to the environment's environmental sustainability. Likewise, economic diversification will mitigate environmental degradation and improve formal employment. Our findings are relevant to policymakers and researchers interested in promoting ecological sustainability., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Though construction sector development and economic openness contribute to regional economic development, they have also been debated to pose some environmental challenges. Along these lines, we explored the long- and short-term connections of intensive energy consumption, economic openness, and construction sector development with the chemical oxygen demand throughout the scales of regional development of China's 30 provincial units over the 2004-2021 period. Theoretically, we contribute to the existing knowledge by incorporating chemical oxygen demand pollution, construction sector development, and economic openness to the Kaya identity's baseline framework. Empirically, we apply a series of advanced methods of panel data econometrics for robust results. Our key findings are as follows: First, we revealed a long-term stable cointegrating association among our variables of interest. Second, using the common correlated effect mean group estimator, we unfolded that the intensive energy consumption showed a chemical oxygen demand pollution reduction influence in both the long and short term, demonstrating the most substantial influence in the high regional development panel while expressing the least powerful influence the least regional development setting. Third, we unveiled that economic openness and construction sector development showed a linear chemical oxygen demand pollution enhancement influence in moderately and least developed regions. Nevertheless, both established an inverted U-shaped linkage with chemical oxygen demand pollution for the whole country as well as for high regional development data samples. Eventually, we found consistent estimates across long- and short-term investigations regarding signs of relationships; however, long-term effects remained more powerful than short-term ones. These findings would serve as factual scientific knowledge to help local as well as national governments create the optimal environmental regulations for the construction sector to achieve the sustainable development goals (SDGs), especially the Climate Action Plan (i.e., SDG-13)., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

The leaf worm, Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) is a notorious insect pest of many economically important cultivated crops like cotton, maize, tobocco and vegetables causing severe economic losses from 50-100%. In most crops, damage arises due to voracious feeding by the larvae and leads to the skeletonizing of leaves. Toxicological studies were performed to estimate lethal and sublethal levels of flubendiamide and spirotetramat against S. litura . Effects of these estimated values were assessed on different biological traits of S. litura including life duration, survival and next generation potential. Both flubendiamide and spirotetramat showed toxic responses against second instar larvae of S. litura under laboratory conditions. Larval duration and survival rate of S. litura to were significantly different. Exposure to test insecticides resulted in negative effect on the demography of S. litura as longer life cycle and decreased fecundity. Changes in net reproductive rate and intrinsic rate of increase also helped to decide the fate of these insecticides. Low reproductive potential and low hatching percentage due to exposure to test insecticides can help to manage next generation of target pest. These two new chemistry insecticides can be recommended for their effective and long-term utilization against this important leaf feeder which may help its management and decrease economic losses faced by the growers. Their impact on larval duration and low survival rate at lethal levels guides about their potential in pest control., Competing Interests: Muhammad Imran and Junaid Rahim are Academic Editors for PeerJ., (©2023 un-Nisa et al.)

This study examines the impact of government spending, income, and tourism consumption on CO 2 emissions in the 50 US states through a novel theoretical model derived from the Armey Curve model and the Environmental Kuznets Curve hypothesis. The findings of this research are essential for policymakers to develop effective strategies for mitigating environmental pollution. Utilizing panel cointegration analysis, the study provides valuable insights into whether continued increases in government spending contribute to higher pollution levels. By identifying the threshold point of spending as a percentage of GDP, policymakers can make informed decisions to avoid the trade-off between increased spending and environmental degradation. For instance, the analysis reveals that Hawaii's tipping point is 16.40%. The empirical results underscore the importance of adopting sustainable policies that foster economic growth while minimizing environmental harm. These findings will aid policymakers in formulating targeted and efficient approaches to tackle climate change and promote long-term environmental sustainability in the United States. Moreover, the impact of tourism development on CO 2 emissions varies across states, with some US states experiencing a decrease while others see an increase., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

While economic development-driven anthropogenic emissions pose challenges to ecological sustainability, the international travel and tourism sector has appeared as a hot contestant to bring sustainability to the ecological systems across varying development levels. This work investigates the diversified effects of the international travel and tourism sector and economic development on ecological deterioration, in the presence of urban agglomeration and energy use efficiency, across the development levels of China's 30 provincial units from 2002 to 2019. It contributes in two ways. (i) The stochastic estimation of environmental impacts by regression on population, affluence, and technology (STIRPAT) is modified to integrate the variables like international travel and tourism sector, urban agglomeration, and energy use efficiency. (ii) We measured an international travel and tourism sector index (ITTI) and made use of a continuously updated bias correction strategy (CUBCS) and a continuously updated fully modified strategy (CUFMS) for the long-term estimations. Besides, we used the bootstrapping-based causality technique for determining causality directions. The core results are as follows: Firstly, ITTI and economic development produced an inverse U-type association with ecological deterioration for the aggregate panels. Secondly, provinces exhibited a diverse range of links in that ITTI mitigated (boosted) the ecological deterioration in eleven (fourteen) provinces presenting diversified shapes of linkages. Economic development established the environmental Kuznets curve (EKC) theory with ecological deterioration in merely four provinces; however, the non-EKC theory is verified in twenty-four divisions. Thirdly, in China's east zone (high development scale), the ITTI revealed the ecological deterioration reduction (promotion) impact in eight (two) provinces. China's central zone (moderate development scale) exhibited ecological deterioration promotion in half of the provinces, and the other half showed a reduction impact. In China's west zone (low development scale), it promoted ecological deterioration in eight provinces. Economic development promoted (reduced) ecological deterioration in a single (nine) province(s). In China's central zone, it boosted (mitigated) the ecological deterioration in five (three) provinces. In China's west zone, it promoted (reduced) ecological deterioration in eight (two) provinces. Fourthly, urban agglomeration and energy use efficiency deteriorated and improved the environmental quality in aggregated panels, respectively; however, a diverse range of effects are observed for provinces. Finally, a unilateral bootstrap causality, from ITTI (economic development) to ecological deterioration, is revealed in twenty-four (fifteen) provinces. A bilateral causality is established in a single (thirteen) province(s). Based on empirical findings, policies are suggested., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Background: This study aimed to determine the fit of two small-sized (pediatric and infant) continuous-flow total artificial heart pumps (CFTAHs) in congenital heart surgery patients., Methods: This study was approved by Cleveland Clinic Institutional Review Board. Pediatric cardiac surgery patients ( n  = 40) were evaluated for anatomical and virtual device fitting (3D-printed models of pediatric [P-CFTAH] and infant [I-CFTAH] models). The virtual sub-study consisted of analysis of preoperative thoracic radiographs and computed tomography ( n  = 3; 4.2, 5.3, and 10.2 kg) imaging data., Results: P-CFTAH pump fit in 21 out of 40 patients (fit group, 52.5%) but did not fit in 19 patients (non-fit group, 47.5%). I-CFTAH pump fit all of the 33 patients evaluated. There were critical differences due to dimensional variation ( p  < 0.0001) for the P-CFTAH, such as body weight (BW), height (Ht), and body surface area (BSA). The cutoff values were: BW: 5.71 kg, Ht: 59.0 cm, BSA: 0.31 m 2 . These cutoff values were additionally confirmed to be optimal by CT imaging., Conclusions: This study demonstrated the range of proper fit for the P-CFTAH and I-CFTAH in congenital heart disease patients. These data suggest the feasibility of both devices for fit in the small-patient population., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (© 2023 Miyagi, Ahmad, Karimov, Polakowski, Karamlou, Yaman, Fukamachi and Najm.)

Environmental sustainability investigation has been a hotly debated topic of the modern literature; however, past studies have primarily overlooked its transmissibility or spillover outreach across economies. Herein, we investigate the novel aspect of whether transportation-based environmental taxation spatially induces spillover impacts across Italy, Germany, and France over the 1994-2020 period by employing a simultaneous spatial equation with multi-country dynamic stochastic general equilibrium modeling (DSGEM) framework. Transportation-based environmental taxation of the domestic economy negatively impacts its own investment and consumption, while it impacts the economy of neighboring economies positively. Change in output and investment in the domestic economy can be well explained by the environmental volatility of the domestic economy, whereas the environmental volatility of neighboring economies does not contribute much to explain the change in investment and output of the domestic economy. Volatility in pollution discharge occurs more by environmental volatility in the neighboring economy than in the domestic economy, and validating that environmental sustainability is transmissible across regions and economies. It urgently calls for environmental protection policies integrated and coordinated across the countries and regions to spread and capitalize on environmentally and economically favorable and sustainable effects globally. Achieving the spatially transmitted positive environmental and economic outcomes would help strengthen the Sustainable Development Goals (SDG), with a particular focus on Climate Action (SDG13), Sustainable Production and Consumption (SDG12), and Affordable and Sustainable Energy for All (SDG7)., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

The aim of the current study is to design the radiolabeled and drug-loaded nanocarrier with high loading capacity and pH-dependent drug release characteristics that could effectively transport loaded compounds to various organs for efficient diagnostic imaging and chemotherapeutic drug delivery. The aqueous extract of green tea leaves was used to synthesize the small-sized iron oxide nanoparticles (IONPs). The nanoparticles were characterized with UV-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and energy-dispersive X-ray analysis (EDX). Iron oxide nanoparticles with sizes smaller than 50 nm were successfully synthesized, making them suitable for in vivo studies. In drug loading trials, 94% of the drug was loaded onto the active surface of iron oxide nanoparticles from the solution. The in vitro drug release study revealed that an acidic environment (pH 4.5) effectively triggers the release of doxorubicin (DOX) from the nanoparticles as compared to a neutral environment (pH 7.4). The gamma-emitting radionuclide 99m Tc was successfully labeled with IONPs for biodistribution and imaging studies. The efficiency of radiolabeling was observed to be ≥ 99%. Furthermore, the in vivo biodistribution study of radiolabeled IONPs in rabbit model showed rapid accumulation in various organs such as heart, liver, and kidneys. This work suggested that green synthesized iron oxide nanoparticles are potential nanocarriers for diagnostic imaging and efficiently distributing DOX to specific organs. The aqueous extract of green tea leaves was used for the facile green synthesis of iron oxide nanoparticles (IONPs). Furthermore, the chemotherapeutic drug doxorubicin (DOX) and gamma-emitting radionuclide 99m Tc were loaded on these iron oxide nanoparticles to evaluate the in vivo biodistribution and drug delivery studies in the rabbit models., (© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)

Converting carbon-based molecular fuels into electricity efficiently and cleanly without emitting CO 2 remains a challenge. Conventional fuel cells using noble metals as anode catalysts often suffer performance degradation due to CO poisoning and a host of problems associated with CO 2 production. This study provides a CO 2 -emission-free direct formaldehyde fuel cell. It enables a flow of electricity while producing H 2 and valuable formate. Unlike conventional carbon-based molecules electrooxidation, formaldehyde 1-electron oxidation is performed on the Cu anode with high selectivity, thus generating formate and H 2 without undergoing CO 2 pathway. In addition, the fuel cell produces 0.62 Nm 3 H 2 and 53 mol formate per 1 kWh of electricity generated, with an open circuit voltage of up to 1 V and a peak power density of 350 mW cm -2 . This study puts forward a zero-carbon solution for the efficient utilization of carbon-based molecule fuels that generates electricity, hydrogen and valuable chemicals in synchronization., (© 2023 Wiley-VCH GmbH.)

This study aims to demonstrate the impact of renewable energy consumption (REC) on environmental degradation using the EKC hypothesis testing for the BRIC and G-7 countries. Two EKC models were created and tested, with Model 2 including REC and other independent variables such as economic freedom (EF) and economic policy uncertainty (EPU), which affect the level of renewable energy consumption and CO 2 emissions. Empirical findings indicate that the EKC hypothesis is verified faster in the REC-EF-EPU-based EKC model (Model 2) than in the EF-EPU-based EKC model (Model 1) for G-7 countries since the turning point takes place earlier in Model 2 than in Model 1 with REC. This suggests that renewable energy consumption accelerates the reduction of CO 2 emissions. Moreover, this earlier turning point results in lower environmental cleaning costs, less time vesting, and saving resources and money for G-7 countries. However, the study found no evidence supporting the EKC hypothesis for the BRIC countries., Competing Interests: Conflicts of InterestThe authors declare no conflict of interest. The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.)

We looked at the long-term and short-term diversified relationships between industrial agglomeration, aggregate energy consumption, residential construction sector growth, and air pollution in China's 30 provincial units from 2004 through 2020. We contributed to the existing knowledge by calculating a holistic air pollution index (API) and applying advanced methods. We also augmented the Kaya identity by including industrial agglomeration and residential construction sector growth in the baseline framework. Based on empirical results: First, we revealed long-term stability among our covariates through panel cointegration analysis. Second, we uncovered a positive bilateral relationship between residential construction sector growth and industrial agglomeration in the long and short term. Third, we unfolded a unilateral positive correlation emerging from aggregate energy consumption to API, displaying the greatest influence in the east zone of China. Fourth, we observed a unilateral positive connection stemming from industrial agglomeration and residential construction sector growth to aggregate energy consumption and API in the long- and short-term dimensions. Finally, the linking nature was homogeneously prevailing across the long term and short term; however, the long-term impact size outweighed that of the short term. Given our empirical results, useful policy insights are discussed to provide the readers with a take-home message for substantiating sustainable development goals., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Background: A button battery can cause perforation of Meckel's diverticulum if sequestered in this blind ending bowel segment. Reported cases focused on clinical manifestations, management options, radiologic and intraoperative findings. We highlight the histopathologic features in perforated Meckel's diverticula due to ingested button batteries. Case report: Three toddlers presented with perforated Meckel's diverticula after button battery ingestion. Histologic examination of the diverticula showed multiple burn-like mucosal ulcerations and liquefactive wall perforations of the tips, associated with prominent siderotic pigment deposits and calcifications. There were various residual materials with variable staining patterns in the luminal debris, necrotic tissue and adjacent to submucosal blood vessels. Conclusion: There is accelerated deposition of iron and calcium associated with button batteries.

We aim to analyze the existence of a novel agricultural production-augmented environmental Kuznets curve (EKC-AP) theory for energy transition, urban agglomeration, and financial depth. To conduct this research, we use a global panel of selected 54 countries belonging to low, medium, and high levels of economic development from 1971 to 2017. Since our data are distributed into three development levels, it is highly likely to face cross-sectional dependence across the countries within and between the development levels, which may provide biased empirical results. To outpace such possibility, we employ advanced econometric strategies, which are robust to cross-sectional dependence and slope heterogeneity issues. We found that agricultural production, energy transition, urban agglomeration, financial depth, and ecological degradation experience a long-term cointegrating equilibrium association. We also confirm that exists an EKC-AP linkage of agricultural production with ecological degradation in highly developed economies, while those at the low and medium levels of development reveal a positive exponential and monotonic impact, respectively, of agricultural production on ecological degradation. The energy transition is found to promote ecological sustainability in developed countries through technique impact, while it deteriorates the ecological quality in countries at the medium development level. Moreover, urban agglomeration adversely impacts the ecological quality in the economies at low and medium development levels, whereas it improves the ecological sustainability in developed economies. Besides, financial depth proves harmful to ecological sustainability, with less subtle effects in developed economies. Based on our findings, for countries at low and medium levels of development, we propose advancing farming techniques using ecologically friendly technologies, promoting green energy transition, expanding the share of the services sector, and providing green financial systems to support green investment projects to achieve the Sustainable Development agenda of the United Nations., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

For the first time, this study introduces-proposes using the Armey curve hypothesis (ACH) for testing the environmental Kuznets curve hypothesis (EKCH) in the relevant literature. The rationale for this new proposed methodology is that both hypotheses are expected to have similar inverted U-shaped curves. Hence, we combine the aforementioned hypotheses to obtain a single composite model. This single model may allow us to calculate a maximum (optimum) level of government expenditures that will increase or decrease CO 2 emissions for USMCA (the USA-Mexico-Canada Agreement) countries. To this end, our study employs an augmented mean group (AMG) estimator. The results demonstrate that the EKCH is verified by way of the AC model only for Mexico. Additionally, with the advantage of this approach, we calculated the optimal government spending level, which will increase both per capita real GDP (RGDPPC) and CO 2 emissions in this country by around 26.4% of RGDPPC. This level of spending will be a kind of threshold point for the Mexican government's policymakers. Hence, they will know that if they continue to spend more than this level, both the RGDPPC and CO 2 emissions will decrease, implying either a lower RGDPPC or a cleaner environment. The primary purpose of the proposed methodology in this study is to reveal the possible effects of the government's economic growth-oriented increased public expenditures on the environment in a single composite model. In other words, the relationship between economic growth and the environment is approached from the perspective of public spending, and it is reminded that governments should have harmonious and sustainable public spending policies for both economic growth and a cleaner environment., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)