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ABSTRACTRice (Oryza sativa L.) stands out as the world's most vital staple food crop, yet is susceptible to UV-B radiation stress. This study investigates the physiological and transcriptome responses in rice exposed to titanium dioxide nanoparticles (TiO2 NPs) under UV-B radiation. Results demonstrate that TiO2 NPs, applied alone (TN) or in combination with UV-B stress (UV+TN), significantly enhance rice plant growth and physiological parameters. Reactive oxygen species (ROS) levels, elevated under UV-B stress, are significantly reduced by TN and UV+TN treatments, thereby regulating antioxidants particularly involved in ascorbate-glutathione pathway. Transcriptomics analysis of identified DEGs in UV+TN, utilizing KEGG pathway analysis, reveals significant enrichment in various pathways. These pathways include glutathione metabolism, pyruvate metabolism, starch and sucrose metabolism, regulation of basal transcription factors, plant hormonal signal transduction pathways, cellular processes associated with energy, and the MAPK signaling pathway. Overall, TiO2 NPs application modulates diverse biological and metabolic pathways, enhancing UV-B stress tolerance in rice.

Abstract Fungal endophytes in plant leaf mesophyll form mutually beneficial associations through carbon assimilation, synthesis of biologically active chemicals, and enhancement of aesthetic and nutritional value. Here, we compared community structure, diversity, and richness of endophytic fungi in the leaves of three bamboo species, including Phyllostachys edulis (MZ), Bambusa rigida (KZ), and Pleioblastus amarus (YT) via high-throughput Illumina sequencing. In total, 1070 operational taxonomic units (OTUs) were retrieved and classified into 7 phylum, 27 classes, 82 orders, 185 families, 310 genus, and 448 species. Dominant genera were Cladosporium, Trichomerium, Hannaella, Ascomycota, Sporobolomyces, Camptophora and Strelitziana. The highest fungal diversity was observed in Pleioblastus amarus, followed by Bambusa rigida, and Phyllostachys edulis. Comparatively, monopodial species Ph. edulis and sympodial B. rigida, mixed P. amarus revealed the highest richness of endophytic fungi. We retrieved a few biocontrol agents, Sarocladium and Paraconiothyrium, and unique Sporobolomyces, Camptophora, and Strelitziana genera. FUNGuild analysis revealed the surrounding environment (The annual average temperature is between 15 and 25 °C, and the relative humidity of the air is above 83% all year round) as a source of fungal accumulation in bamboo leaves and their pathogenic nature. Our results provide precise knowledge for better managing bamboo forests and pave the way for isolating secondary metabolites and potential bioactive compounds.

Abstract Silicon (Si) and/or proline (Pro) are natural supplements that are considered to induce plants' stress tolerance against various abiotic stresses. Sweet corn (Zea mays L. saccharata) production is severely afflicted by salinity stress. Therefore, two field tests were conducted to evaluate the potential effects of Si and/or Pro (6mM) used as seed soaking (SS) and/or foliar spray (FS) on Sweet corn plant growth and yield, physio-biochemical attributes, and antioxidant defense systems grown in a saline (EC = 7.14dS m−1) soil. The Si and/or Pro significantly increased growth and yield, photosynthetic pigments, free proline, total soluble sugars (TSS), K+/Na+ratios, relative water content (RWC), membrane stability index (MSI), α-Tocopherol (α-TOC), Ascorbate (AsA), glutathione (GSH), enzymatic antioxidants activities and other anatomical features as compared to controls. In contrast, electrolytes, such as SS and/or FS under salt stress compared to controls (SS and FS using tap water) were significantly decreased. The best results were obtained when SS was combined with FS via Si or Pro. These alterations are brought about by the exogenous application of Si and/or Pro rendering these elements potentially useful in aiding sweet corn plants to acclimate successfully to saline soil.

Purpose The present study was carried out to evaluate anti-inflammatory and antiangiogenic attributes of simvastatin and its nanofilms containing silver nanoparticles.Methods Silver nanoparticles and simvastatin-loaded nanocomposite (SNSN) films were formulated by using polymeric solution (pectin + sericin) through casting solution method. Different in vitro and in vivo anti-inflammatory assays were performed. In addition, chick chorioallantoic membrane assay (CAM) was also employed for angiogenesis activity.Results FTIR spectra of the film depicted the presence of intact simvastatin. Differential scanning calorimetry exhibited no endothermic expression in F9 film thermogram. The simvastatin release from all films exhibited a burst effect. Cotton-pellet induced granuloma model study showed that high dose of simvastatin and indomethacin produced comparable (p

IntroductionOsmoprotectant supplementation can be used as a useful approach to enhance plant stress tolerance. However, the effect of silymarin and clove fruit extract (CFE) on wheat plants grown under cadmium (Cd) stress has not been studied.MethodsWheat seeds were planted in plastic pots filled with ions-free sand. A ½-strength Hoagland’s nutrient solution was used for irrigation. Pots were treated with eight treatments thirteen days after sowing: 1) Control, 2) 0.5 mM silymarin foliar application [silymarin], 3) 2% CFE foliar application [CFE], 4) CFE enriched with silymarin (0.24 g silymarin L-1 of CFE) [CFE-silymarin], 5) Watering wheat seedlings with a nutritious solution of 2 mM Cd [Cd]. 6) Cadmium + silymarin, 7) Cadmium + CFE, and 8) Cadmium + CFE-silymarin. The experimental design was a completely randomized design with nine replicates. Results and discussionThe Cd stress decreased grain yield, shoot dry weight, leaf area, carotenoids, chlorophylls, stomatal conductance, net photosynthetic rate, transpiration rate, membrane stability index, nitrogen, phosphorus, and potassium content by 66.9, 60.6, 56.7, 23.8, 33.5, 48.1, 41.2, 48.7, 42.5, 24.1, 39.9, and 24.1%, respectively. On the other hand, Cd has an Application of CFE, silymarin, or CEF-silymarin for wheat plants grown under Cd stress, significantly improved all investigated biochemical, morphological, and physiological variables and enhanced the antioxidant enzyme activities. Applying CFE and/or silymarin enhanced plant tolerance to Cd stress more efficiently. Our findings suggest using CFE-silymarin as a meaningful biostimulator for wheat plants to increase wheat plants’ tolerance to Cd stress via enhancing various metabolic and physiological processes.

Overseeded perennial ryegrass (Lolium perenne L.) turf on dormant bermudagrass (Cynodon dactylon Pers. L) in transitional climatic zones (TCZ) experience a severe reduction in its growth due to cold stress. Primary plant nutrients play an important role in the cold stress tolerance of plants. To better understand the cold stress tolerance of overseeded perennial ryegrass under TCZ, a three-factor and five-level central composite rotatable design (CCRD) with a regression model was used to study the interactive effects of nitrogen (N), phosphorus (P), and potassium (K) fertilization on lipid peroxidation, electrolyte leakage, reactive oxygen species (ROS) production, and their detoxification by the photosynthetic pigments, enzymatic and non-enzymatic antioxidants. The study demonstrated substantial effects of N, P, and K fertilization on ROS production and their detoxification through enzymatic and non-enzymatic pathways in overseeded perennial ryegrass under cold stress. Our results demonstrated that the cold stress significantly enhanced malondialdehyde, electrolyte leakage, and hydrogen peroxide contents, while simultaneously decreasing ROS-scavenging enzymes, antioxidants, and photosynthetic pigments in overseeded perennial ryegrass. However, N, P, and K application mitigated cold stress-provoked adversities by enhancing soluble protein, superoxide dismutase, peroxide dismutase, catalase, and proline contents as compared to the control conditions. Moreover, N, P, and, K application enhanced chlorophyll a, chlorophyll b, total chlorophyll, and carotenoids in overseeded perennial ryegrass under cold stress as compared to the control treatments. Collectively, this 2−years study indicated that N, P, and K fertilization mitigated cold stress by activating enzymatic and non-enzymatic antioxidants defense systems, thereby concluding that efficient nutrient management is the key to enhanced cold stress tolerance of overseeded perennial ryegrass in a transitional climate. These findings revealed that turfgrass management will not only rely on breeding new varieties but also on the development of nutrient management strategies for coping cold stress.

Tomato yellow leaf curl virus (TYLCV) is one of the most harmful afflictions in the world that affects tomato growth and production. Six regular antagonistic genes (Ty-1, Ty-2, Ty-3, Ty-4, ty-5, and Ty-6) have been transferred from wild germplasms to commercial cultivars as TYLCV protections. With Ty-1 serving as an appropriate source of TYLCV resistance, only Ty-1, Ty-2, and Ty-3 displayed substantial levels of opposition in a few strains. It has been possible to clone three TYLCV opposition genes (Ty-1/Ty-3, Ty-2, and ty-5) that target three antiviral safety mechanisms. However, it significantly impacts obtaining permanent resistance to TYLCV, trying to maintain opposition whenever possible, and spreading opposition globally. Utilizing novel methods, such as using resistance genes and identifying new resistance resources, protects against TYLCV in tomato production. To facilitate the breeders make an informed decision and testing methods for TYLCV blockage, this study highlights the portrayal of typical obstruction genes, common opposition sources, and subatomic indicators. The main goal is to provide a fictitious starting point for the identification and application of resistance genes as well as the maturation of tomato varieties that are TYLCV-resistant.

The popularity of date palm (Phoenix dactylifera) fruit is increasing, therefore the demand for high-quality date palm fruit with less or no chemical treatment is the topic of interest for date producers and consumers. The quality of date palm fruit is much dependent on its postharvest handling and processing. For preventing the degradation and maintenance of the high quality of dates during the storage an appropriate harvest and post-harvest processes are required. The process should control the biotic and abiotic factors like insects, fungus, temperature, as well as handling and processing of dates. Therefore, in this work, we reviewed the literature related to the protection of date fruits during their post-harvest life. The commercially viable advance and updated techniques that can be used to avoid storage losses and problems while keeping fruit quality (nutritional, color, flavor, and texture) and microbial safety under optimal conditions are discussed.

Shading is one of the most chronic restrains which can lead to the lodging of intercropped plants. In order to increase the soybean stem lodging resistance, a 2-year field trial was conducted to evaluate the impact of different ratios and concentrations of NH4+/NO3– on the morpho-physiological and biochemical characteristics of soybean stem under shade conditions. The total 5 ratios of NH4+/NO3– were applied as follows: T0 = 0/0 (control), T1 = 0/100 (higher ratio), T2 = 25/75 (optimum), T3 = 50/50 (optimum), and T4 = 75/25 (higher ratio) as a nitrogen source. Our findings displayed that the T2 (25/75) and T3 (50/50) treatments alleviated the shading stress by improving the photosynthetic activity, biomass accumulation, carbohydrates contents, and lignin related enzymes (POD, CAD, and 4Cl) which led to improvement in stem lodging resistance. The correlation analysis (p ≤ 0.05, p ≤ 0.01) revealed the strong relationship between lodging resistance index and stem diameter, stem strength, lignin content, photosynthesis, and lignin related enzymes (POD, CAD, and 4CL) evidencing the strong contribution of lignin and its related enzymes in the improvement of lodging resistance of soybean stem under shade conditions. Collectively, we concluded that optimum NH4+/NO3– ratios (T2 and T3) can boost up the lodging resistance of soybean stem under shade stress.

Healthcare workers in Pakistan are still fighting at the frontline to control the spread of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and have been identified as the earliest beneficiaries for COVID-19 vaccination by the health authorities of the country. Besides, the high vaccination rates of frontline healthcare workers (FHWs) are essential to overcome the ongoing pandemic and reduce the vaccines hesitancy among the general population. The current research employed the theory of planned behavior (TPB) to investigate the COVID-19 vaccination behavior among FHWs in Pakistan as well as the predictors of such behavior. Following the epidemic control and prevention policies, a sample of 680 FHWs were accessed to fill in the questionnaire evaluating the components of the TPB. Moreover, the potential role of anticipated regret (AR) and perceived susceptibility (PS) on COVID-19 vaccination behavior was also assessed. The partial least square structural equation modeling (PLS-SEM) results revealed that the TPB components, as well as the AR, have positive associations with the COVID-19 vaccination behavior. The results further confirmed that PS positively affects the anticipated regret, attitude (ATT), and subjective norm (SN) to vaccinate against SARS-CoV-2. The perceived susceptibility also has a positive association with COVID-19 vaccination behavior through the mediation of anticipated regret, ATT, and SN. Our findings highlighted the importance of COVID-19 vaccination among healthcare workers, which can be applied to reduce vaccine hesitancy among the general public.

Syntheses and characterization (1H & 13C NMR and CHN) of four new thiosemicarbazone derivatives (1–4) are reported. The molecular structures of compounds 1–4 were determined using single crystal X-ray crystallographic technique. Cytotoxic effect of all the compounds was determined against three cancerous (PANC-1, HCT 116 and MCF-7) and one non-cancerous (NIH/3T3) cell lines. All the compounds remained safe against non-cancerous cells (IC50 > 500 μM) but induced significant cytotoxicity in the cancerous cell lines. Importantly, compounds 2 and 4 showed excellent cytotoxicity (IC50 = 10.0 and 0.7 μM against PANC-1; 14.9 and 9.4 μM against HCT 116; 14.3 and 15.8 μM against MCF-7 for 2 and 4 respectively). Based on the results it can be concluded that the thiosemicarbazone derivatives (2 and 4) can be further studied in detail for the purpose of chemotherapeutic drug development. Keywords: Thiosemicarbazones, Crystal structures, Antitumor agents, Bioorganic chemistry, Cytotoxicity

The interstock, a stock between the rootstock and scion, has a significant regulatory effect on the stock and scion, and its function is highly dependent on graft compatibility. To assess the graft compatibility of the interstock and scion, ‘Yuanxiaochun’ was top grafted onto ‘Ponkan’, ‘Shiranuhi’, ‘Harumi’, ‘Tarocco’, and ‘Kumquat’. The results showed significant differences in the survival ratio and preservation ratio among different combinations. Grafting compatibility in the early stages of grafting was associated with the genetic relationship. The biomass accumulation revealed that the interstock could influence both the rootstock and the scion. The physiological and biochemical traits analysis suggested that SPAD reading and soluble sugar could be employed as preselected indices to evaluate graft compatibility in the late stage of grafting. These results indicated that the evaluation of graft compatibility was a dynamic process. The findings provided a new approach for studying the stock and scion interaction mechanisms mediated by interstock, and directly provided a theoretical and practical basis for the high-grafting of ‘Yuanxiaochun’ citrus.

The emergence of coronavirus disease 2019 (COVID-19) has considerably changed global food production, processing, and consumption at different levels. Sojourners are among those who have experienced a higher level of food insecurity during the crisis of the COVID-19 outbreak. The current research aimed to investigate the immediate consumption behavioral intentions of the Pakistani international students in the People's Republic of China (PRC) during the wake of COVID-19 pandemic. This study applied the Theory of Planned Behavior (TPB) and background factors of food safety and health consciousness that influence the consumption behavioral intention of Pakistani students toward unfamiliar local food in China. A relational model was analyzed where food safety and health consciousness were hypothesized to serve as background variables associated with TPB components. Moreover, the indirect effects of food safety and health consciousness on behavioral intentions were assessed. The data were collected through convenience samples from 462 Pakistani international students and were analyzed through partial least square structural equation modeling (PLS-SEM). The results confirmed that food safety and health consciousness were positively associated with attitude (ATT), subjective norm (SN), and perceived behavioral control (PBC). However, food safety and health consciousness were indirectly associated with the behavioral intention only through ATT and SN. The results highlighted the role of food safety and health consciousness as important antecedents of classical TPB components that affect intentions and behaviors to avoid unfamiliar local food in a migrated context. The present study provides enlightenment to those who aim to investigate the consumption behavioral intentions of sojourners in the wake of the pandemic situation based on food safety and health consciousness. The findings of the current study are also applicable to general consumption patterns in the food sector.

Global climate change may result in major environmental issues that have already affected and will continue to affect agricultural sector in the future. A continuing effort to utilize and adopt new agricultural technologies is necessary to mitigate climate change and increase agricultural income. Agricultural cooperatives are gradually being used in emerging countries to encourage improved technology and reduce food insecurity and poverty. This research analyses the influence of cooperative supports (CS) and technology adoption (TA) on agricultural income in Pakistan. It applied the propensity score matching (PSM) technique to evaluate the productivity on survey data from 498 wheat growers to conduct counterfactual analysis for farmers in Pakistan. In addition, a dual selection model (DSM) was applied to resolve the bias in sample selection caused by observed and unobserved aspects of survey data. The results showed that, contrasted with non-membership and non-adopters, growers who joined CS and TA could boost agricultural income by 2.78% and 2.35%, respectively. Stimulatingly, the influence of less-revenue farmers on agricultural income was more substantial than that of high-income farmers. Agricultural income of growers who attached cooperatives and adopted improved agricultural technology enhanced by 5.45% and 4.51%, respectively. These results, among others, emphasize the optimistic role of growing CS and TA in boosting wheat farmer’s income. The findings of the study showed strong relationships among education, age, skill, training, gender with CS and TA, and agricultural income. Overall, this study can be helpful in conducting similar studies in other emerging/developing countries for wheat or any other crop growers.

Peach fruit and trees are prone to various issues, regarding yield, quality and pest attack. Calcium plays several roles in plant and fruit development. Therefore the current study was conducted to evaluate the response of peach fruit to foliar application. The experiment was laid out in Randomized Complete Block Design with two factors factorial arrangement and three replicates. Calcium chloride was applied at the rate of 0, 1, 2 and 3%. The foliar application of calcium was done at pink bud stage, berry size fruit stage, and then at pit hardening stage of peach. The obtained resulted indicated that foliar application of calcium significantly improved peach fruit quality and yield. The highest fruit weight (142.6 g), yield tree–1 (15.6 kg), ascorbic acid content (6.67 mg‧100 g–1), total soluble solids (11.0°Bx), fruit juice pH (3.98), sugar to acid ratio (45.1), fruit firmness (3.90 kg‧cm–2) and fruit volume (155.6 cm3) was obtained with the application of 3% solution of calcium chloride at pit hardening stage but it significantly reduced the percent titratable acidity (0.3%) and disease incidence (5.8%). It was concluded that 3% calcium chloride applied at the pit hardening stage significantly boosted peach quality and fruit yield.

Salmonella Typhi is a Gram-negative pathogen that causes typhoid fever in humans. The use of antibiotics to treat typhoid has considerably mitigated its fatality risk, but rising multidrug-resistant (MDR) and extensively drug-resistant (XDR) resistance in Pakistan threatens effective treatment. This study determined the prevalence of MDR and XDR S. Typhi at a local hospital in Lahore. Blood samples (n = 3000) were obtained and processed for bacterial identification. Antibiotic susceptibility test was performed using VITEK® 2 Compound 30 System. Statistical data analysis was performed using a Mann–Whitney U and Kruskal–Wallis H test, respectively. The results revealed 600 positive cultures, of which the majority were found to be XDR S. Typhi (46.1%) and MDR S. Typhi (24.5%) strains. The disease burden of resistant Salmonella strains was greater in males (60.67%) than females (39.33%), with the most affected age group being 0–10 years old (70.4 %). In both the outpatient department (OPD) and general ward, the prevalence of XDR S. Typhi cases was found to be alarmingly high (48.24%), followed by MDR S. Typhi (25.04 %). The results of the statistical analysis demonstrated that the incidence of resistance in MDR and XDR S. Typhi strains was not affected by the age as well as the gender of patients (p > 0.05). The occurrence of resistant strains against four tested antibiotics (azithromycin, ciprofloxacin, imipenem, and meropenem) was found to be similar in different wards and among hospitalized and OPD patients (p > 0.05). Maximum resistance was observed against chloramphenicol and ampicillin in the OPD and pediatric ward. Piperacillin/Tazobactam was observed to be the most effective antibiotic, followed by co-amoxiclav (p < 0.001). This study is effective in validating the existence of MDR and XDR S. Typhi in Lahore, where stringent methods should be applied for controlling its spread.

Advanced technologies and innovation are essential for promoting sustainable food systems (SFSs) because these technologies can be used to answer some of the critical questions needed to transform SFSs and help us better understand global food security and nutrition. The main objective of this study is to address the question of whether technological innovations have an impact on the transformation of SFSs. There are certain innovations including agricultural land utilization, food processing, production systems, improvement in diets according to people’s needs, and management of waste products. This study provides an overview of new technologies and innovations being used with potential to transform SFSs. Applications of emerging technologies in digital agriculture, including the Internet of Things (IoT), artificial intelligence and machine learning, drones, use of new physical systems (e.g., advanced robotics, autonomous vehicles, advanced materials), and gene technology (e.g., biofortified crops, genome-wide selection, genome editing), are discussed in this study. Additionally, we suggest eight action initiatives, which are transforming mindsets, enabling social licensing, changing policies and regulations, designing market incentives, safeguarding against undesirable effects, ensuring stable finance, building trust, and developing transition pathways that can hasten the transition to more SFSs. We conclude that appropriate incentives, regulations, and social permits play a critical role in enhancing the adoption of modern technologies to promote SFSs.

Increasing agricultural production and optimizing inorganic fertilizer (IF) use are imperative for agricultural and environmental sustainability. Mobile phone usage (MPU) has the potential to reduce IF application while ensuring environmental and agricultural sustainability goals. The main objectives of this study were to assess MPU, mobile phone promotion policy, and whether the mediation role of human capital can help reduce IF use. This study used baseline regression analysis and propensity score matching, difference-in-differences (PSM-DID) to assess the impact of MPU on IF usage. However, the two-stage instrumental variables method (IVM) was used to study the effects of mobile phone promotion policy on IF usage. This study used a national dataset from 7987 rural households in Afghanistan to investigate the impacts of MPU and associated promotion policies on IF application. The baseline regression outcomes showed that the MPU significantly reduced IF usage. The evaluation mechanism revealed that mobile phones help reduce IF application by improving the human capital of farmers. Besides, evidence from the DID technique showed that mobile phone promotion policies lowered IF application. These results remained robust after applying the PSM-DID method and two-stage IVM to control endogenous decisions of rural households. This study results imply that enhancing the accessibility of wideband in remote areas, promoting MPU, and increasing investment in information communication technologies (ICTs) infrastructure can help decrease the IF application in agriculture. Thus, the government should invest in remote areas to facilitate access to ICTs, such as having a telephone and access to a cellular and internet network to provide an environment and facility to apply IF effectively. Further, particular policy support must focus on how vulnerable populations access the internet and mobile phone technologies.

The advancement in nanotechnology has brought numerous benefits for humans in diverse areas including industry, medicine, and agriculture. The demand in the application of nanomaterials can result in the release of these anthropogenic materials into soil and water that can potentially harm the environment by affecting water and soil properties (e.g., soil texture, pH, organic matter, and water content), plants, animals, and subsequently human health. The properties of nanoparticles including their size, surface area, and reactivity affect their fate in the environment and can potentially result in their toxicological effects in the ecosystem and on living organisms. There is extensive research on the application of nano-based materials and the consequences of their release into the environment. However, there is little information about environmentally friendly approaches for removing nanomaterials from the environment. This article provides insight into the application of silver nanoparticles (AgNPs), as one of the most commonly used nanomaterials, their toxicological effects, their impacts on plants and microorganisms, and briefly reviews the possibility of remediation of these metabolites using phytotechnology approaches. This article provides invaluable information to better understand the fate of nanomaterials in the environment and strategies in removing them from the environment.

Cadmium (Cd) stress causes serious disruptions in plant metabolism, physio-biochemical responses, crop yield, and grain quality characteristics. A pot experiment was conducted to investigate the role of molybdenum (Mo) in mitigating Cd-induced adversities on plant growth, yield attributes, and grain quality characteristics of a popular aromatic rice cultivar ‘Xiangyaxiangzhan’. The Mo was applied at 0.15 mg kg−1 soil in both control (no Cd) and Cd-contaminated (100 mg kg−1) soils. A treatment with Mo-free (−Mo) soil was also maintained for comparison. The results showed that Cd toxicity significantly (p < 0.05) reduced plant dry biomass, grain yield, photosynthetic efficiency, and pigment contents, and impaired chloroplast ultra-structural configuration and simultaneously destabilized the plant metabolism owing to higher accumulation of hydrogen peroxide, electrolyte leakage, and malondialdehyde contents. However, Mo supply improved grain yield and 2-acetyl-1-pyrroline content by 64.75% and 77.09%, respectively, under Cd stress, suggesting that Mo supply mitigated Cd-provoked negative effects on yield attributes and grain quality of aromatic rice. Moreover, Mo supply enhanced photosynthesis, proline, and soluble protein content, and also strengthened plant metabolism and antioxidant defense through maintaining higher activities and transcript abundance of ROS-detoxifying enzymes at the vegetative, reproductive, and maturity stages of aromatic rice plants under Cd toxicity. Collectively, our findings indicated that Mo supply strengthened plant metabolism at prominent growth stages through an improved enzymatic and non-enzymatic antioxidant defense system, thereby increasing grain yield and quality characteristics of aromatic rice under Cd toxicity.

Crop yield can be raised by establishment of adequate plant stand using seeds with high germination ratio and vigor. Various pre-sowing treatments are adopted to achieve this objective. One of these approaches is the exposure of seeds to a low-to-medium level magnetic field (MF), in pulsed and continuous modes, as they have shown positive results in a number of crop seeds. On the basis of the sensitivity of plants to MF, different types of MF have been used for magnetopriming studies, such as weak static homogeneous magnetic fields (0–100 μT, including GMF), strong homogeneous magnetic fields (milliTesla to Tesla), and extremely low frequency (ELF) magnetic fields of low-to-moderate (several hundred μT) magnetic flux densities. The agronomic application of MFs in plants has shown potential in altering conventional plant production systems; increasing mean germination rates, and root and shoot growth; having high productivity; increasing photosynthetic pigment content; and intensifying cell division, as well as water and nutrient uptake. Furthermore, different studies suggest that MFs prevent the large injuries produced/inflicted by diseases and pests on agricultural crops and other economically important plants and assist in reducing the oxidative damage in plants caused by stress situations. An improved understanding of the interactions between the MF and the plant responses could revolutionize crop production through increased resistance to disease and stress conditions, as well as the superiority of nutrient and water utilization, resulting in the improvement of crop yield. In this review, we summarize the potential applications of MF and the key processes involved in agronomic applications. Furthermore, in order to ensure both the safe usage and acceptance of this new opportunity, the adverse effects are also discussed.

Bermudagrass [Cynodon dactylon (L.) Pers.] turf loss due to severe cold in transitional climates is a major concern. To overcome this problem, warm-season grass is often overseeded with a cool-season turfgrass. In this study, modeling and efficient nutrient management were used to evaluate this problem. A three-factor and five-level central composite rotatable design (CCRD) with a simulation of a regression model was used to optimize fertilization rates. The study investigated the combined effects of fertilization with nitrogen (N), phosphorus (P), and potassium (K) on both the morphological and physiological attributes and on the integrated turf performance (ITP) of overseeded perennial ryegrass (Lolium perenne). Fertilization with N and P significantly increased turf height, density, color, fresh and dry weights, while N, P, and K significantly affected turf cover, quality and winter-kill. The Spring transition was delayed by fertilization with N and P, and accelerated by fertilization with K. Photosynthesis (Pn), transpiration (Tr), and stomatal conductance (Gs) were considerably enhanced by fertilization with N, P, and K. Protein levels and total chlorophyll levels were substantially increased by fertilization with N and P and with N, P, and K, respectively, during a 2-year period. During two separate experiments conducted during 2 consecutive years, the optimal combinations of N, P, and K were N: 30, P: 24, K: 9, and N: 30, P: 27, K: 6 g m−2. The major conclusion of this study is that a balanced nutrient application utilizing N, P, and K is key to enhancing the winter performance of perennial ryegrass.

OBJECTIVE: The present study aimed to investigate the mechanisms underlying the anti-inflammatory and anti-angiogenic effects of ethyl-p-methoxycinnamate isolated from Kaempferia galanga. METHODS: The anti-inflammatory effects of ethyl-p-methoxycinnamate were assessed using the cotton pellet granuloma assay in rats, whereby the levels of interleukin-1 and tumor necrosis factor-α were measured in the animals' blood. In addition, the levels of interleukin, tumor necrosis factor, and nitric oxide were measured in vitro using the human macrophage cell line (U937). The analgesic effects of ethyl-p-methoxycinnamate were assessed by the tail flick assay in rats. The anti-angiogenic effects were evaluated first by the rat aortic ring assay and, subsequently, by assessing the inhibitory effects of ethyl-p-methoxycinnamate on vascular endothelial growth factor, proliferation, migration, and tube formation in human umbilical vein endothelial cells. RESULTS: Ethyl-p-methoxycinnamate strongly inhibited granuloma tissue formation in rats. It prolonged the tail flick time in rats by more than two-fold compared with the control animals. The inhibition of interleukin and tumor necrosis factor by ethyl-p-methoxycinnamate was significant in both in vivo and in vitro models; however, only a moderate inhibition of nitric oxide was observed in macrophages. Furthermore, ethyl-p-methoxycinnamate considerably inhibited microvessel sprouting from the rat aorta. These mechanistic studies showed that ethyl-p-methoxycinnamate strongly inhibited the differentiation and migration of endothelial cells, which was further confirmed by the reduced level of vascular endothelial growth factor. CONCLUSION: Ethyl-p-methoxycinnamate exhibits significant anti-inflammatory potential by inhibiting pro-inflammatory cytokines and angiogenesis, thus inhibiting the main functions of endothelial cells. Thus, ethyl-p-methoxycinnamate could be a promising therapeutic agent for the treatment of inflammatory and angiogenesis-related diseases.

This study evaluated the anti-inflammatory effect of Kaempferia galanga (KG) using an activity-guided approach. KG rhizomes were serially extracted with petroleum ether, chloroform, methanol and water. These extracts (2 g/kg each) were tested for their ability to inhibit carrageenan-induced rat paw edema. The chloroform extract was found to exert the highest inhibition (42.9%) compared to control (p < 0.001), hence it was further fractionated by washing serially with hexane, hexane-chloroform (1:1) and chloroform. The chloroform fraction (1 g/kg) showed the highest inhibitory effect (51.9%, p < 0.001) on carrageenan-induced edema. This chloroform fraction was further fractionated with hexane-chloroform (1:3) and chloroform, and of the two fractions, the hexane-chloroform sub-fraction was the most effective in inhibiting edema (53.7%, p < 0.001). GC-MS analysis of the active sub-fraction identified ethyl-p-methoxycinnamate (EPMC) as the major component, which was re-crystallized. EPMC dose-dependently inhibited carrageenan-induced edema with an MIC of 100 mg/kg. Moreover, in an in vitro study, EPMC non-selectively inhibited the activities of cyclooxygenases 1 and 2, with IC50 values of 1.12 µM and 0.83 µM respectively. These results validate the anti-inflammatory activity of KG which may be exerted by the inhibition of cyclooxygenases 1 and 2. EPMC isolated from this plant may be the active anti-inflammatory agent.