15 results on '"Muhammad Amjad Awan"'
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2. Evaluation of carboxylated poly‐ <scp>l</scp> ‐lysine for cryopreservation of Labeo rohita sperm
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Shaista Bibi, Muhammad Amjad Awan, Bushra Allah Rakha, Rabea Ejaz, Saima Qadeer, Javeria Arshad, Muhammad Zubair Anjum, and Shamim Akhter
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Endocrinology ,Animal Science and Zoology ,Biotechnology - Published
- 2023
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3. Evaluation of extenders for refrigerated preservation ofLabeo rohitamilt
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Bushra Allah Rakha, Shaista Bibi, Sana Urooj, Shamim Akhter, Muhammad Amjad Awan, Muhammad Sajjad Ansari, Muhammad Zubair Anjum, Javeria Arshad, and Rabea Ejaz
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Labeo ,Milt ,biology ,law ,Extender ,%22">Fish ,Food science ,Aquatic Science ,biology.organism_classification ,Cryopreservation ,law.invention - Published
- 2021
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4. Effect of Synergism Between Carboxylated Poly-l-Lysine and Glycerol on Freezability of Nili-Ravi Buffalo (Bubalus bubalis) Semen
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Muhammad Amjad Awan, Javeria Arshad, Shamim Akhter, Sajid Iqbal, Muhammad Sajjad Ansari, and Bushra Allah Rakha
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Cryoprotectant ,Lysine ,Medicine (miscellaneous) ,Semen ,complex mixtures ,General Biochemistry, Genetics and Molecular Biology ,law.invention ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,law ,Glycerol ,Food science ,030219 obstetrics & reproductive medicine ,biology ,Extender ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,Cell Biology ,General Medicine ,biology.organism_classification ,040201 dairy & animal science ,Sperm ,chemistry ,Antifreeze ,bacteria ,Bubalus - Abstract
Carboxylated poly-l-lysine (CPLL), an ampholytic polymer, has remarkable cryoprotective properties. It was hypothesized that CPLL will reduce/replace glycerol in extender and improve the freezabili... more...
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- 2020
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5. Effect of Quercetin on Oxidative Stress, Mitochondrial Activity, and Quality of Indian Red Jungle Fowl (Gallus gallus murghi) Sperm
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Bushra Allah Rakha, Muhammad Sajjad Ansari, Ali Akhter, Julián Santiago-Moreno, Qurrat-Ul-Ain, Shamim Akhter, and Muhammad Amjad Awan
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animal structures ,Fowl ,Extender ,Medicine (miscellaneous) ,Indian Red ,Cell Biology ,General Medicine ,Biology ,medicine.disease_cause ,biology.organism_classification ,Sperm ,General Biochemistry, Genetics and Molecular Biology ,law.invention ,Andrology ,Lipid peroxidation ,Gallus gallus murghi ,chemistry.chemical_compound ,chemistry ,law ,medicine ,heterocyclic compounds ,Quercetin ,Oxidative stress - Abstract
Aim: The study was designed to elucidate the effects of quercetin in an extender on oxidative stress, mitochondrial activity and quality of Indian red jungle fowl (Gallus gallus murghi) sperm durin... more...
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- 2020
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6. Antioxidant effect of Moringa olifera leaves extract in extender improves post‐thaw quality, kinematics, lipid peroxidation, total antioxidant capacity and fertility of water buffalo bull semen
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Muhammad Fahad Bhutta, Sadia Naz, Muhammad Amjad Awan, Abubakar Sufyan, and Sajid Iqbal
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Buffaloes ,Urology ,Semen ,Antioxidants ,Cryopreservation ,law.invention ,Moringa ,Lipid peroxidation ,Semen quality ,chemistry.chemical_compound ,Cryoprotective Agents ,Endocrinology ,Animal science ,law ,Animals ,Acrosome ,Sperm motility ,Plant Extracts ,Extender ,General Medicine ,Spermatozoa ,Biomechanical Phenomena ,Plant Leaves ,Semen Analysis ,chemistry ,Sperm Motility ,Lipid Peroxidation ,Semen Preservation - Abstract
Our objectives were to determine the antioxidant effect of Moringa olifera leave extract (MLE) in tris extender on post-thaw quality, kinematics, lipid peroxidation, total antioxidant capacity and field fertility of water buffalo bull semen. Semen was collected on a weekly basis for 5 weeks (n = 5 bulls & n = 25). After collection, every sample was diluted individually with extender (tris egg yolk) containing MLE concentrations (0%, 5%, 10%, 15% and 20%) at 37°C. After dilution, semen was filled in 0.54 mL straws and frozen in automatic freezer. After thawing, sperm motility (%) (progressive & total), path velocity (µm/s), straight velocity (µm/s), curvilinear velocity (%), beat cross frequency (Hz), linearity (%), straightness (%), length of average path (µm), length of straight-line path (µm), length of curvilinear path (µm), plasma membrane integrity (%), acrosome membrane integrity (%), DNA integrity (%) and morphology (%) were higher (p < 0.05) in spermatozoa preserved in extender containing 15% MLE as compared to control. The fertility rate (60%, vs. 45%) was higher (p < 0.05) with semen doses frozen with 15% MLE in extender than the control. In conclusion, 15% MLE improves semen quality, kinematics and field fertility in water buffaloes. more...
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- 2021
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7. Honey as an Alternative to Antibiotics for Cryopreservation of Nili-Ravi Buffalo Bull Spermatozoa
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Shiza Nasreen, William V. Holt, Muhammad Sajjad Ansari, Asma Ul-Husna, Bushra Allah Rakha, Muhammad Amjad Awan, and Shamim Akhter
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Male ,Veterinary medicine ,Buffaloes ,Brassica ,Medicine (miscellaneous) ,Acacia ,Semen ,Penicillins ,General Biochemistry, Genetics and Molecular Biology ,Cryopreservation ,03 medical and health sciences ,Cryoprotective Agents ,0302 clinical medicine ,food ,parasitic diseases ,Animals ,030219 obstetrics & reproductive medicine ,biology ,fungi ,0402 animal and dairy science ,food and beverages ,Ziziphus ,Honey ,04 agricultural and veterinary sciences ,Cell Biology ,General Medicine ,biology.organism_classification ,Antimicrobial ,Egg Yolk ,040201 dairy & animal science ,Semen cryopreservation ,food.food ,Anti-Bacterial Agents ,Ziziphus jujuba ,Sperm Motility ,Streptomycin ,Semen Preservation - Abstract
The antimicrobial properties of honey have stimulated interest in evaluating it as an alternative to antibiotics for cryopreserved buffalo semen. Acacia nilotica, Brassica campestris and Ziziphus j... more...
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- 2020
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8. Asphaltum Improves the Post-thaw Quality and Antioxidant Status of Nili Ravi Buffalo Bull Sperm
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Shamim Akhter, Bushra Allah Rakha, Sadia Naz, Jaweria Sultan, Syeda Anila Waqar, Muhammad Amjad Awan, Muhammmad Sajjad Ansari, and Sajid Iqbal
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Male ,Antioxidant ,Ayurvedic medicine ,Buffaloes ,medicine.medical_treatment ,Nili-Ravi ,Medicine (miscellaneous) ,General Biochemistry, Genetics and Molecular Biology ,Antioxidants ,03 medical and health sciences ,0302 clinical medicine ,Cryoprotective Agents ,medicine ,Animals ,Shilajit ,Cryopreservation ,030219 obstetrics & reproductive medicine ,Traditional medicine ,biology ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,Cell Biology ,General Medicine ,biology.organism_classification ,040201 dairy & animal science ,Sperm ,Semen cryopreservation ,Spermatozoa ,Sperm Motility ,Panacea (butterfly) ,Semen Preservation - Abstract
Asphaltum, a mineral exudate from the mountains, is an ayurvedic medicine believed to be a panacea for male reproductive health issues. The objective of the study was to evaluate asphaltum in terms of phytochemical components, radical scavenging activity (RSA) more...
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- 2021
9. Sperm binding to hyaluronan is an excellent predictor of Nili-Ravi buffalo bull fertility
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Ali A. Fouladi-Nashta, Muhammad Waseem, Bushra Allah Rakha, Shamim Akhter, Muhammad Sajjad Ansari, David Miller, Javeria Arshad, and Muhammad Amjad Awan
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Male ,endocrine system ,Buffaloes ,Urology ,Sperm binding ,media_common.quotation_subject ,Nili-Ravi ,Semen ,Fertility ,Cryopreservation ,Andrology ,Endocrinology ,Cryoprotective Agents ,Animals ,Humans ,Hyaluronic Acid ,Acrosome ,reproductive and urinary physiology ,media_common ,biology ,urogenital system ,High fertility ,General Medicine ,biology.organism_classification ,Sperm ,Spermatozoa ,Sperm Motility ,Semen Preservation - Abstract
This study reports the first evaluation of sperm hyaluronan binding assay (HBA) for predicting the fertility of Nili-Ravi buffalo bulls in relation to standard parameters of sperm quality. Cryopreserved semen doses of low (n = 6), medium (n = 3) and high fertility (n = 8) bulls based on their respective return rates were used. Significantly, more spermatozoa bound to hyaluronan from the most fertile bulls (57.15% ± 1.44) compared with medium (42.46% ± 1.08) and low fertility bulls (29.70% ± 0.78). A strongly positive correlation (r = .824, p more...
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- 2020
10. Effect of Synergism Between Carboxylated Poly-l-Lysine and Glycerol on Freezability of Nili-Ravi Buffalo (
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Shamim, Akhter, Muhammad Amjad, Awan, Javeria, Arshad, Bushra Allah, Rakha, Muhammad Sajjad, Ansari, and Sajid, Iqbal
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Glycerol ,Male ,Cryoprotective Agents ,Buffaloes ,Semen ,Sperm Motility ,Animals ,Polylysine ,Spermatozoa ,Semen Preservation - Abstract
Carboxylated poly-l-lysine (CPLL), an ampholytic polymer, has remarkable cryoprotective properties. It was hypothesized that CPLL will reduce/replace glycerol in extender and improve the freezability of buffalo semen. The objective was to evaluate various combinations of CPLL and glycerol in extenders for any synergism toward cryosurvivability of Nili-Ravi buffalo sperm. Semen collected from four Nili-Ravi buffalo bulls (ejaculates = 40; replicates = 5) was diluted in tris-citric acid based extenders, Control: (0% CPLL +7% Glycerol); E1: (1% CPLL +6% Glycerol); E2: (2% CPLL +5% Glycerol); E3: (3% CPLL +4% Glycerol); E4: (4% CPLL +3% Glycerol); E5: (5% CPLL +2% Glycerol); E6: (6% CPLL +1% Glycerol), and E7: (7% CPLL +0% Glycerol), and cryopreserved using a programmable cell freezer. Percentages of post-thaw sperm motility, plasma membrane integrity, and acrosomal integrity were found to be higher ( more...
- Published
- 2020
11. PSXII-17 Fertility prediction model for Nilli-Ravi buffalo bulls through Fourier harmonic analysis of sperm
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Shamim Akhter, Muhammad Amjad Awan, Javeria Arshad, and John J. Parrish
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endocrine system ,urogenital system ,media_common.quotation_subject ,Fertility ,General Medicine ,Sperm ,Poster Presentations ,Harmonic analysis ,symbols.namesake ,Fourier transform ,Statistics ,Genetics ,symbols ,Animal Science and Zoology ,reproductive and urinary physiology ,Food Science ,Mathematics ,media_common - Abstract
A model to predict Nili-ravi buffalo bull fertility was developed based on Fourier harmonic analysis of sperm. Seventeen bulls with 3032 AI records were categorizes based on fertility rate (FR) as low (36.5±0.2, n = 6: SD< ˗1 from mean FR), medium (39.9±0.2, n = 3; SD +1 to -1 from mean FR) and high fertility (41.4±0.1, n = 8; SD > +1 from mean FR). Cryopreserved semen samples from these bulls were investigated for Fourier harmonic analysis of sperm nuclear shape. Hoechst-33342 and YOYO-1 fluorescent stains were used to identify live and dead sperm. Digital images were analyzed to get sperm nuclear perimeter points at different phase angles to generate Fourier functions. Mean harmonic amplitude (HA) 0 was different (P < 0.05) for 1700 live vs. 1294 dead sperm from the 17 bulls, thus live sperm were used for remaining analyses. The mean, variance, skewness and kurtosis values of 100 live sperm nuclei/bull were compared for HA0-5 between high (n = 6) and low (n = 6) fertility groups, considering equal number of bulls in each category. The mean HA2 (0.739±0.01 vs 0.686±0.00) and 4 (0.105±0.001 vs 0.007±0.001) were higher in high vs low fertility group respectively (P < 0.05). Sperm nuclear perimeter among high fertility group sperm was more elongated. There was also an increased skewness of HA0 as fertility increased (P < 0.05). Discriminant analysis defined a fertility model by using mean HA4, skewness HA0 and variance HA2, that resulted in 91.7% bulls into their correct fertility group upon cross-validation (canonical correlation=0.928; P < 0.05). Higher values of mean HA4, skewness HA0 and variance HA2 increase the chance of bulls being placed in the high fertility group. In conclusion, sperm nuclear shape in Nili-ravi buffalo bull is related to in vivo fertility. A fertility model using reported discriminant measures could be used to objectively identify Nili-ravi buffalo bulls of varying fertility. more...
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- 2021
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12. Sperm preparation through Sephadex™filtration improves in vitro fertilization rate of buffalo oocytes
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S Nasreen, A.U. Husna, Qaisar Shahzad, Asima Azam, Shamim Akhter, S. Qadeer, Muhammad Amjad Awan, Muhammad Khalid, and Ali A. Fouladi-Nashta
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030219 obstetrics & reproductive medicine ,In vitro fertilisation ,Chemistry ,medicine.medical_treatment ,0402 animal and dairy science ,Motility ,Semen ,04 agricultural and veterinary sciences ,040201 dairy & animal science ,Sperm ,Cryopreservation ,Andrology ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Human fertilization ,Sephadex ,medicine ,Animal Science and Zoology ,Centrifugation ,Biotechnology - Abstract
Routinely, swim-up method is used to separate high-quality sperm; however, long processing time and close cell-to-cell contact during the centrifugation step are inevitable elements of oxidative stress to sperm. The objective was to evaluate Sephadex™ and glass wool filtration to separate motile, intact and viable sperm for in vitro fertilization in buffalo. The cumulus-oocyte complexes (COCs) were collected from ovaries of slaughtered buffaloes by aspiration and matured for 24 hr in CO2 incubator at 38.5°C and 5% CO2 . Matured COCs were rinsed twice in fertilization TALP and placed in the pre-warmed fertilization medium without sperm. Cryopreserved buffalo semen was thawed at 37°C for 30 s and processed through Sephadex™ , glass wool filtration and swim-up (control). Total and motile sperm recovery rates were assessed, resuspended in fertilization TALP and incubated for 15-20 min in CO2 incubator. Samples prepared by each method were divided into two aliquots: one aliquot was studied for sperm quality (progressive motility, membrane integrity, viability, liveability), while the other was subjected to co-incubation with sets of 10-15 in vitro matured oocytes. Data on sperm quality were analysed by ANOVA, while in vitro fertilizing rates were compared by chi-squared test using SPSS-20. Least significant difference (LSD) test was used to compare treatment means. Glass wool filtration yielded higher total and motile sperm recovery rate, while Sephadex™ filtration improved (p < .05) sperm quality (progressive motility, membrane integrity, viability, liveability). Sperm preparation through Sephadex filtration yielded higher in vitro fertilization rate in terms of cleavage rate compared to glass wool filtration and swim-up (control). In conclusion, cryopreserved Nili-Ravi buffalo sperm selected through Sephadex filtration showed improved quality and yielded better fertilization rates (cleavage rate) of in vitro matured/fertilized oocytes. Sephadex filtration could be a promising technique for use in in vitro fertilization in buffalo. more...
- Published
- 2017
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13. Sperm sexing in Nili-Ravi buffalo through modified swim up: Validation using SYBR ® green real-time PCR
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Muhammad Sajjad Ansari, Abid Mehmood, Asma-Ul-Husna, Bushra Allah Rakha, S. M. Saqlan Naqvi, Muhammad Amjad Awan, Tasawar Sultana, Shamim Akhter, and Qaisar Shahzad
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Genetics ,030219 obstetrics & reproductive medicine ,urogenital system ,Extender ,0402 animal and dairy science ,Semen ,04 agricultural and veterinary sciences ,General Medicine ,Sexing ,Biology ,Y chromosome ,040201 dairy & animal science ,Sperm ,Cryopreservation ,law.invention ,Andrology ,03 medical and health sciences ,Semen quality ,0302 clinical medicine ,Endocrinology ,Food Animals ,law ,Animal Science and Zoology ,X chromosome - Abstract
Sperm sexing through flow-sorting technology is relatively expensive, requires considerable technical support and is actually not practicable in many developing countries. The aim of this study was to investigate the feasibility of producing enriched pools of X or Y chromosome-bearing sperm by a modified swim-up method. For this purpose semen was collected from five mature Nili-Ravi buffalo bulls for a period of six weeks. The qualifying ejaculates were divided into two aliquots for further processing through modified swim-up or control (untreated). After processing, semen was cryopreserved in tris citric acid extender using standard techniques. Semen quality was assessed at pre dilution, post dilution and post thawing. Validation of technique was done by using SYBR® green real time PCR using two sets of primers, PLP and SRY for X and Y chromosome of buffalo genes, respectively. Sperm recovery rates, pre freeze and post thaw sperm quality were found significantly higher in X chromosome bearing sperm fraction than Y chromosome bearing fraction and control. Mean fold relative expression of X bearing sperm was significantly higher (4-5 fold) in X chromosome bearing fraction of supernatant than Y chromosome bearing fraction (0.06 fold), similarly mean fold relative expression of Y chromosome bearing sperm was significantly higher in Y chromosome bearing fraction (4 fold) of supernatant than X chromosome bearing fraction (0.15 fold) compared to control (1.00). In conclusion, a modified swim up method proved to be an effective method for Nili-Ravi buffalo sperm sexing as validated by real time PCR. more...
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- 2017
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14. Carboxylated poly-l-lysine (CPLL) as a replacement of egg yolk in extender for Nili Ravi buffalo bull sperm
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Javeria Arshad, Shamim Akhter, Bushra Allah Rakha, Muhammad Amjad Awan, and Muhammad Sajjad Ansari
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food.ingredient ,biology ,Chemistry ,Nili-Ravi ,Lysine ,Extender ,General Medicine ,biology.organism_classification ,Sperm ,General Biochemistry, Genetics and Molecular Biology ,law.invention ,food ,law ,Yolk ,Food science ,General Agricultural and Biological Sciences - Published
- 2020
- Full Text
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15. Sperm sexing in Nili-Ravi buffalo through modified swim up: Validation using SYBR
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Asma-Ul-Husna, Muhammad Amjad, Awan, Abid, Mehmood, Tasawar, Sultana, Qaisar, Shahzad, Muhammad Sajjad, Ansari, Bushra Allah, Rakha, S M, Saqlan Naqvi, and Shamim, Akhter
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Cryopreservation ,Male ,X Chromosome ,Buffaloes ,Reproducibility of Results ,Real-Time Polymerase Chain Reaction ,Spermatozoa ,Semen Analysis ,Cryoprotective Agents ,Y Chromosome ,Sperm Motility ,Animals ,Female ,Sex Preselection - Abstract
Sperm sexing through flow-sorting technology is relatively expensive, requires considerable technical support and is actually not practicable in many developing countries. The aim of this study was to investigate the feasibility of producing enriched pools of X or Y chromosome-bearing sperm by a modified swim-up method. For this purpose semen was collected from five mature Nili-Ravi buffalo bulls for a period of six weeks. The qualifying ejaculates were divided into two aliquots for further processing through modified swim-up or control (untreated). After processing, semen was cryopreserved in tris citric acid extender using standard techniques. Semen quality was assessed at pre dilution, post dilution and post thawing. Validation of technique was done by using SYBR more...
- Published
- 2017
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