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Siling Ren,1,* Jingxian Wu,2,* Wanchun Yin,1 Qianqian Liao,1 Sailan Gong,1 Beibei Xuan,1 Xiaoling Mu1 1Department of Gynecology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, People’s Republic of China; 2Department of Pathology, Chongqing Medical University, Chongqing 400016, People’s Republic of China*These authors contributed equally to this work.Correspondence: Xiaoling MuDepartment of Gynecology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, People’s Republic of ChinaEmail mxl@hospital.cqmu.edu.cnObjective: In this study, 345 patients with endometrial carcinoma (EC) were selected to investigate the correlation between ER/PR status and the EC disease-free survival (DFS) rate.Methods: The intensity and proportion of tumor cell expression of estrogen receptors and progesterone receptors (ER/PR) status of 345 postoperative tumor specimens in ECs were independently assessed semi-quantitatively by two pathologists using immunohistochemistry, the summed score ranged from 0 to 8 points was worked out by adding proportion score and intensity score based on the breast cancer hormone receptor immunohistochemical Allred scoring system. The association between DFS in ECs and ER/PR expression (intensity, proportion and summed score) was assessed using Cox regression analysis. Gene expression data were obtained from The Cancer Genome Atlas research network (TCGA).Results: According to inclusion criteria, 201 type I and 144 type II EC patients were enrolled in this study. In the univariate analysis of type I endometrial carcinoma, the intensity, proportion and summed score of ER/PR status were significantly correlated with DFS. After adjusting for factors known to significantly impact survival, the influence of ER/PR status on DFS is generally decreased but the correlation is still significant. In the univariate analysis of type II endometrial carcinoma, the intensity, proportion and summed score of ER/PR status were significantly correlated with DFS. After adjusting for factors known to significantly impact survival, the influence of ER status on DFS is generally decreased, but the correlation is still significant, the effect of PR expression on DFS is not statistically significant.Conclusion: Higher ER/PR expression status was associated with better DFS in patients with type I endometrial cancer after adjusting for known factors that significantly affect survival. In patients with type II endometrial cancer, patients with positive ER expression were significantly associated with better DFS. However, the effect of PR expression on DFS was not statistically significant.Keywords: endometrial cancer, endometrioid carcinoma, disease-free survival rate, hormone receptor status, ER, PR

BackgroundThere is a lack of literature on short latency period (SLP) in twin pregnancies with preterm premature rupture of membranes (PPROM). Thus, the aim of this study was to identify the clinical factors and perinatal outcomes associated with SLP in twin pregnancies with PPROM and to establish a predictive model to identify SLP.MethodsTwin pregnancies with PPROM between 24 0/7 and 33 6/7 weeks were included and a retrospective analysis was performed. Patients were divided into two groups based on the latency period after PPROM: Group 1 ≤24 h (defined as SLP) and Group 2 >24 h (defined as long latency period, LLP), the clinical factors and perinatal outcomes were compared between the two groups. Binary logistic regression and receiver operating characteristic curve analyses were used to identify the independent clinical factors associated with latency period after PPROM and assess the predictive accuracy for SLP.Results98 and 92 pregnant women had short and long latency period, respectively. Prolonged latency significantly increased the occurrence of chorioamnionitis. Neonatal outcomes were not affected by latency duration after PPROM. Binary regression analysis revealed that higher gestational age (GA) at PPROM (P = 0.038), presence of uterine contractions (P < 0.001), Bishop score > 4 (P = 0.030), serum procalcitonin levels ≥0.05 ng/mL upon admission, and absence of use of tocolytic agents (P < 0.001) were significant independent predictors of a SLP. A predictive model developed using these predictors had an area under the curve (AUC) of 0.838, and the presence of uterine contractions alone had an AUC of = 0.711.ConclusionUterine contraction was the most important prognosticator for a SLP. A latency period of >24 h was associated with chorioamnionitis, but adverse neonatal outcomes were not observed.

Background Epithelial ovarian cancer (EOC) is the most lethal gynaecological malignancy, and there is a deficiency of information in the literature on the early recognition of short-term survivor (STS). This study aimed to identify the clinicopathological factors associated with STS in late-stage EOC and to establish a predictive model to identify STS. Methods Selected patients with International Federation of Gynecology and Obstetrics (FIGO) stage III or IV EOC were included in the study, and a retrospective analysis was performed. The characteristics of the patients who survived not more than 2 years (STS) were compared to those who survived at least 2 years (defined as long-term survivors, LTS). Binary logistic regression and receiver operating characteristic curve (ROC) were used to identify the independent prognostic factors associated with EOC and assess the predictive accuracy for STS. Results We identified 254 patients with advanced EOC including 57 STS and 197 LTS. A univariate analysis revealed that STS had a tendency to have omental metastasis and larger tumor size, to be platinum resistant, to have non-serous histology, to undergo suboptimal cytoreduction, to have comorbidity, and to undergo primary chemotherapy less than 6 courses. Binary regression analysis revealed that tumor size (P=0.033), platinum resistance (P

The water environmental capacity (WEC) in northern rivers has obvious temporal dynamic characteristics because of the seasonal change, so the calculated WEC under a single hydrological designed condition cannot reflect these changes. In this paper, one-dimensional WEC model is used to calculate the pollutant carrying capacity under three different design hydrological conditions of the Yinghe River, which belongs to Yellow River Basin. The results show that: The COD and NH3-N capacity under the staging design condition are increased 7.15% and 9.75% compared with the conventional method; the COD and NH3-N capacity of average monthly flow condition are increased 4.89% and 6.35% compared with the conventional methods; the calculation of the stage design condition is closed to the average monthly flow condition, the COD and NH3-N capacity are increased only 2.14% and 3.20% respectively. Comparing the calculation results under different design hydrological conditions, it is found that the results under the conditions of considering the dynamic characteristics can reflect the actual change law of WEC more accurately and effectively. This research has a guiding significance for realizing the total distribution and seasonal utilization of WEC in the Yinghe River.

Effects of curcumin on invasion and metastasis in the human ovarian cancer cells SKOV3 and approach if this inhibitory effects are related with CXCL12–CXCR4 axis were carefully studied. SKOV3 cells were treated with 10, 25, 50 ìmol/l curcumin for 24, 48 and 72 h. Proliferation of SKOV3 cells was measured with 3-(4,5-demethy-2-thiazolyl)-2,5- dephenyl-2H-tetrazolium-bromide (MTT) assay. Invasion and metastasis of SKOV3 cells were evaluated with transwell chamber. The expressions of CXCL-12 and CXCR4 were detected by western-blot. After being treated with curcumin, the proliferation of SKOV3 cells was inhibited in a time and dose-dependent manner (P < 0.05). In invasion assay, the number of cells in curcumin treated group to migrate to filter coated with Matrigel was reduced compared with the control group (P < 0.05). Meanwhile, in migration assay, the number of cells in curcumin treated group to migrate to filter was also decreased compared with the control group (P

Cu/MgO catalysts with different Cu particle sizes were prepared by a precipitation-deposition method and investigated for their activity in the transfer dehydrogenation of alcohols to carbonyl compounds. They were highly active for the conversion of primary aliphatic alcohols, e.g. 1-octanol. With increasing Cu particle size from 4.6 to 7.4 nm, the aldehyde yield decreased from 65% to 55%, which showed a size effect. The copper particle abstracted an a-hydrogen from the reactive alkoxide intermediate formed on the surface of MgO and simultaneously catalyzed the hydrogenation of styrene.