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2. Survival of Ugandan infants with subtype A and D HIV-1 infection (HIVNET012)

Author

Eshleman, Susan H., Guay, Laura A., Fleming, Thomas, American writer, Mwatha, Anthony, Mracna, Martin, Becker-Pergola, Graziella, Musoke, Philippa, Mmiro, Francis, and Jackson, J. Brooks

Subjects
Epidemiology -- Statistics, Epidemiology -- Research, HIV patients -- Demographic aspects, HIV patients -- Case studies, HIV patients -- Health aspects, HIV patients -- Care and treatment, HIV infection -- Demographic aspects, HIV infection -- Prevention, HIV infection -- Research, HIV infection -- Health aspects, Immunization of infants -- Care and treatment, Immunization of infants -- Health aspects, Infants, AIDS (Disease) -- Research, Health
Abstract

Research has been conducted on HIV-1-infected infant survival. The survival of Ugandian infants with subtype D and subtype A HIV-1 infection has been compared, and the results indicate that there is no association between HIV-1 subtype and infant survival.

Published
2002

7. Analysis of Length Variation in the V1-V2 Region of env in Nonsubtype B HIV Type 1 from Uganda

Author

Klevytska, Alexandra M., Mracna, Martin R., Guay, Laura, Becker-Pergola, Graziella, Furtado, Manohar, Zhang, Linqi, Jackson, J. Brooks, and Eshleman, Susan H.

Abstract

We optimized an assay for analysis of length variation in the V1-V2 region of HIV-1 env in plasma samples from Uganda. V1-V2 env length variation was analyzed in 31 plasma samples containing subtype A, C, D, or A/D recombinant HIV-1. DNA corresponding to the V1-V2 region was amplified by nested PCR. One of the primers in the second step of the PCR was fluorescently labeled. Successful amplification was confirmed by agarose gel electrophoresis. V1-V2 length variation of PCR products was analyzed with an ABI PRISM 3100 genetic analyzer and GeneScan software. A diversity score was generated for each sample on the basis of the degree of fragment length variation. The V1-V2 region was successfully amplified from 30 of 31 samples. Fragment length analysis was successful for all of those 30 samples. The diversity score and lengths of V1-V2 fragments were unique for each sample. This assay can be used for analysis of V1-V2 length variation in subtypes commonly found in Uganda. This assay may be helpful for studies examining the impact of env length diversity on HIV-1 transmission and pathogenesis in regions where these subtypes are prevalent.

Published
2002
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