18 results on '"Moura-Mendes, Juliana"'
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2. Micobiota asociada con yerba mate comercializada en Paraguay
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Mendoza, Laura, Peralta, Inocencia, Ulke, Gabriela, Gonzalez, Laura, Avalos, Claudia, Ferreira, Francisco, Piris, Laura, Grabowski, Cristhian, Benitez, Alicia, Pizarro, Fernando, Sanchez, Susana, Cazal, Cinthia, Moura Mendes, Juliana, Kohli, Man Mohan, Fernández Rios, Danilo, and Arrua, Andrea Alejandra
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riesgo ,food safety ,hongos ,herbal ,hierbas ,seguridad alimentaria ,inocuidad ,innocuity ,fungi ,General Medicine ,risk - Abstract
Resumen: La yerba mate (Ilex paraguariensis) es una hierba ampliamente consumida que se originó en Paraguay, pero que crece en la región entre el sur de Brasil, el norte de Argentina, Paraguay y Uruguay. Se informa que la planta posee múltiples propiedades medicinales que hacen que la yerba mate sea popular para el consumo en esta región. Actualmente también se considera un producto nutracéutico y un alimento funcional que es consumido en diferentes países del mundo. En medicina popular, se ha reportado científicamente que posee múltiples propiedades. Treinta y nueve marcas de muestras de yerba mate se analizaron mediante placas de dilución en serie y se incubaron a 25°C durante 5 días se determinó la incidencia fúngica. De las 39 muestras analizadas, el 15,4% estuvieron libres de la presencia de hongos y levaduras. El 84,6% restante presentó contaminación con incidencias variables de Aspergillus. Se observó la presencia de Aspergillus secciones nigri, flavi, circumdati, fumigati, wentii, usti, versicolor y Emericella nidulans, siendo la prevalente Aspergillus sección nigri, representando el 65,3%. Teniendo en cuenta los riesgos a la salud asociados con estos hongos, es importante considerar la formulación de regulaciones con respecto al tamaño de las partículas y la presencia de ocratoxinas y aflatoxinas en los productos comerciales de yerba mate. Abstract: Yerba mate (Ilex paraguariensis) is a widely consumed herb which originated in Paraguay but grows widely in the region between Southern Brazil, Northern Argentina, Paraguay, and Uruguay. The plant is reported to possess multiple medicinal properties which makes yerba mate popular for consumption as tea in this region. It is also being considered as a nutraceutical product and a functional food consumed in different countries around the world. In popular medicine, and it has been scientifically reported to possess multiple properties. Thirty-nine brands of yerba mate samples were analyzed by serial dilution plates. and incubated at 25 ° C for 5 days. Based on their morphological characteristics, the fungal incidence and the number of colonies forming units were determined. Of the 39 samples analyzed, 15.4% were free from the presence of fungi and yeasts. The remaining 84.6% of the samples were contaminated with variable contents of the fungi Aspergillus. The presence of Aspergillus sections nigri, flavi, circumdati, fumigati, wentii, usti, versicolor and Emericella nidulans was observed the most prevalent among them was Aspergillus section nigri, representing 65.3%. Considering the health risks associated with these fungi, it is important to consider formulating regulations regarding the particle size and the presence of ochratoxins and aflatoxins in commercial yerba mate products.
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- 2022
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3. Species Identification and Mycotoxigenic Potential of Aspergillus Section Flavi Isolated from Maize Marketed in the Metropolitan Region of Asunción, Paraguay
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Moura-Mendes, Juliana, primary, Cazal-Martínez, Cinthia C., additional, Rojas, Cinthia, additional, Ferreira, Francisco, additional, Pérez-Estigarribia, Pastor, additional, Dias, Nathalia, additional, Godoy, Patrício, additional, Costa, Jéssica, additional, Santos, Cledir, additional, and Arrua, Andrea, additional
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- 2023
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4. α-Pinene: Docking Study, Cytotoxicity, Mechanism of Action, and Anti-Biofilm Effect against Candida albicans
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Bomfim de Barros, Daniela, primary, de Oliveira e Lima, Luanna, additional, Alves da Silva, Larissa, additional, Cavalcante Fonseca, Mariana, additional, Ferreira, Rafael Carlos, additional, Diniz Neto, Hermes, additional, da Nóbrega Alves, Danielle, additional, da Silva Rocha, Walicyranison Plinio, additional, Scotti, Luciana, additional, de Oliveira Lima, Edeltrudes, additional, Vieira Sobral, Marianna, additional, Cançado Castellano, Lúcio Roberto, additional, Moura-Mendes, Juliana, additional, Queiroga Sarmento Guerra, Felipe, additional, and da Silva, Márcia Vanusa, additional
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- 2023
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5. In vitro and ex vivo antibiofilm activity of riparin 1, and its nor and dinor homologs, against dermatophytes
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Medeiros Silva, Risley Nikael, primary, Nóbrega da Rocha, Marcelo Antônio, additional, Silva, Emanuel Pereira, additional, Moura-Mendes, Juliana, additional, Ribeiro-Filho, Jaime, additional, de Sousa, Gabriela Ribeiro, additional, de Souza-Ferrari, Jailton, additional, Barbosa-Filho, José Maria, additional, and de Oliveira Pereira, Fillipe, additional
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- 2023
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6. Antifungal and Antibiofilm Activity of Riparin III against Dermatophytes
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Silva, Emanuel Pereira, primary, Rocha, Marcelo Antônio Nóbrega da, additional, Silva, Risley Nikael Medeiros, additional, Moura-Mendes, Juliana, additional, Sousa, Gabriela Ribeiro de, additional, de Souza-Ferrari, Jailton, additional, Barbosa-Filho, José Maria, additional, Lima, Edeltrudes de Oliveira, additional, and Pereira, Fillipe de Oliveira, additional
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- 2023
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7. Comparación de técnicas de tamizaje de actividad antifúngica de aceites esenciales de especias frente cepas de Aspergillus aisladas de maní (Arachis hypogaea)
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Reyes-Caballero, Yessica Magaliz, primary, Cabrera, Maricel, additional, Cazal-Martínez, Cinthia Carolina, additional, Arrúa-Alvarenga, Andrea Alejandra, additional, and Moura Mendes, Juliana, additional
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- 2022
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8. Estimulación del crecimiento de Thypa dominguensis con bacterias promotoras de crecimiento vegetal
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Cazal Martínez Cinthia Carolina, Arrúa Alvarenga Andrea Alejandra, Samudio Oggero Antonio, Martínez Lourdes, Arrúa Pablo, and Moura Mendes Juliana
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Horticulture ,Phytoremediation ,Inoculation ,Germination ,Biomass ,Bacillus sp ,Biology ,biology.organism_classification ,Bacteria - Abstract
Las especies de Typha sp son utilizadas en tratamientos de fitorremediación de manera exitosa. CEMIT - DGICT - UNA ha propuesto como alternativa a problemática del lago Ypacaraí, el uso de esta planta para su recuperación. El objetivo de este trabajo fue utilizar especies de Bacillus sp. para estimular la germinación de las semillas y aumentar el vigor y crecimiento de las plántulas de Thypa dominguensis. Para ello, se aislaron Bacillus sp. de semillas de Thypa dominguensis y se seleccionaron dos aislados que fueron utilizados para el tratamiento de las mismas mediante la inoculación con suspensiones bacterianas; las semillas fueron sembradas y acondicionadas en casa de vegetación por 30 días, al cabo de los cuales se procedió con las evaluaciones y se observó que las plantas tratadas con laUniversidad Nacional de Asunción. Dirección General de Investigaciones Científicas y Tecnológicas. San Lorenzo, Paraguays bacterias presentaron mejor porcentual de germinación, mayor longitud y biomasa con relación al testigo, demostrando que el tratamiento fue eficiente en la estimulación del crecimiento y aumento de la biomasa.
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- 2020
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9. Trichothecene Genotype Profiling of Wheat Fusarium graminearum Species Complex in Paraguay
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Arrua Alvarenga, Andrea Alejandra, primary, Iehisa Ouchi, Julio César Masaru, additional, Cazal Martínez, Cinthia Carolina, additional, Moura Mendes, Juliana, additional, Colmán, Adans Agustín, additional, Fernández Ríos, Danilo, additional, Arrua, Pablo David, additional, Barboza Guerreño, Claudia Adriana, additional, Kohli, Man Mohan, additional, Ramírez, María Laura, additional, Acuña Ruíz, Ana, additional, Sarmiento, María Magdalena, additional, Ortíz, María Cecilia, additional, Nuñez, Adriana, additional, and Lopez-Nicora, Horacio D., additional
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- 2022
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10. Presence of Aflatoxin M1 in Commercial Milk in Paraguay
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Arrúa, Andrea Alejandra, primary, Arrúa, Pablo David, additional, Moura-Mendes, Juliana, additional, Cazal, Cinthia, additional, Ferreira, Francisco Paulo, additional, Grabowski, Cristhian Javier, additional, Lopez-Nicora, Horacio Daniel, additional, and Fernández Rios, Danilo, additional
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- 2021
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11. In vitroand ex vivoantibiofilm activity of riparin 1, and its norand dinorhomologs, against dermatophytes
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Medeiros Silva, Risley Nikael, Nóbrega da Rocha, Marcelo Antônio, Silva, Emanuel Pereira, Moura-Mendes, Juliana, Ribeiro-Filho, Jaime, de Sousa, Gabriela Ribeiro, de Souza-Ferrari, Jailton, Barbosa-Filho, José Maria, and de Oliveira Pereira, Fillipe
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ABSTRACTDermatophytosis is one of the most frequent superficial mycoses in the world. They are mainly caused by the dermatophytes Trichophyton rubrumand Microsporum canis. Biofilm production is an essential factor in the pathogenesis of dermatophytes; it confers drug resistance and significantly impairs antifungal effectiveness. Therefore, we evaluated the antibiofilm activity of an alkamide-type alkaloid called riparin 1 (RIP1) against clinically relevant dermatophytes. We also produced synthetic nor(NOR1) and dinor(DINOR1) homologs for pharmacological evaluation, with a 61–70% yield. We used invitro(96-well polystyrene plates) and ex vivo(hair fragments) models to verify the effects of these compounds on the formation and viability of biofilms. RIP1 and NOR1 showed antifungal activity against strains of T. rubrumand M. canis, but DINOR1 showed no significant antifungal activity against the dermatophytes. Furthermore, RIP1 and NOR1 significantly reduced the viability of biofilms invitroand ex vivo(P< 0.05). RIP1 was more potent than NOR1, possibly due to the distance between the p-methoxyphenyl and the phenylamide moieties in these compounds. Due to the significant antifungal and antibiofilm activities observed for RIP1 and NOR1, we suggest that they could be useful in the treatment of dermatophytosis.
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- 2023
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12. Presencia de aflatoxina M1 en fórmulas lácteas infantiles comercializadas en el área metropolitana a Asunción, Paraguay.
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Arrúa, Andrea Alejandra, primary, Arrua, Pablo David, additional, Ulke, María Gabriela, additional, Quezada Viay, Martha Yolanda, additional, Moreno Lara, Josefina, additional, Moura Mendes, Juliana, additional, Cazal, Cinthia, additional, Ferreira, Francisco, additional, Kohli, Man Mohan, additional, López Nicora, Horacio, additional, and Fernández Rios, Danilo, additional
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- 2021
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13. Presencia de micotoxinas en preparados y colados comerciales para lactantes en el área metropolitana de Asunción, Paraguay
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Arrúa, Andrea Alejandra, Arrúa, Pablo David, Moura Mendes, Juliana, Paulo Ferreira, Francisco, Fernández Ríos, Danilo, Ulke, María Gabriela, Quezada, Martha Yolanda, Moreno Lara, Josefina, Cazal, Cinthia, Pereira, Mónica Belén, Mellid, Marcos, Peralta López, Inocencia, and Kholi, Man Mohan
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food safety ,alimentación ,toxinas fúngicas ,Food ,contaminación ,inocuidad alimentaria ,pollution ,fungal toxins - Abstract
RESUMEN Introducción: Las micotoxicosis son enfermedades producidas por micotoxinas, metabolitos secundarios tóxicos producidos por hongos filamentosos. Los lactantes son especialmente susceptibles a este tipo de toxinas debido a la inmadurez anatómica y funcional de sus sistemas digestivo e inmune, lo que se refleja en la relación entre la cantidad de alimento ingerida y su peso. Objetivo: Determinar la presencia de micotoxinas en alimentos para lactantes comercializados en farmacias y supermercados del Área Metropolitana. Materiales y métodos: Se colectaron al azar 66 unidades de productos de seis marcas diferentes de preparados y colados comerciales importados, dulces y salados, de farmacias y supermercados del Área Metropolitana. Posteriormente, fueron analizados mediante el ensayo de inmunoafinidad ligado a enzimas (ELISA). Se realizó el análisis de varianza y la posterior comparación de medias de las concentraciones de micotoxinas mediante la prueba de Tukey (IC= 95%), con el estadístico InfoStat®. Resultados: Las micotoxinas prevalentes fueron aflatoxinas (AF) y Toxina T2, que se presentaron en 39% de las unidades muestrales analizadas, tanto en preparados como en colados. En tercer orden de importancia se encuentra ocratoxina A (OTA), detectada en 18% de las mismas. En cuarto lugar, deoxinivalenol (DON) se detectó en 4% los productos. Se presentaron diferencias significativas entre los preparados y los colados, siendo los colados los que en media presentaron niveles más altos de concentraciones de todas las micotoxinas estudiadas. Conclusiones: Se constataron niveles variables de AF, OTA, T2 y DON en los alimentos para lactantes comercializados en el Área Metropolitana. Los colados presentaron concentraciones más elevadas de micotoxinas en media en todos los productos analizados. Tanto AF como OTA superaron los límites máximos permitidos por las normas internacionales. ABSTRACT Introduction: Mycotoxicoses are diseases caused by mycotoxins, secondary toxic metabolites produced by filamentous fungi. Infants are especially susceptible to this type of toxins due to the anatomical and functional immaturity of their digestive and immune systems, which is related to the amount of food eaten and their weight. Objective: To determine the presence of mycotoxins in foods for infants sold in pharmacies and supermarkets in the Metropolitan Area. Materials and methods: 66 units of products from six different brands of imported commercial sweet and salty preparations and strained foods from pharmacies and supermarkets in the Metropolitan Area were randomly collected. Subsequently, they were analyzed by the enzyme-linked immunoaffinity test (ELISA). The variance analysis and the subsequent comparison of means of mycotoxin concentrations were performed using the Tukey test (95% CI), with the InfoStat® statistic. Results: The prevalent mycotoxins were aflatoxins (AF) and T-2 Toxin, which were present in 39% of the sample units analyzed, both in preparations and in strains. In third order of importance we detected Ochratoxin A (OTA) in 18% of the units. Fourth, deoxinivalenol (DON) products were detected in 4%. There were significant differences between the preparations and the strains, with the strains having the highest concentration levels of all the mycotoxins studied. Conclusions: Variable levels of AF, OTA, T-2 and DON were found in infant foods marketed in the Metropolitan Area. The strains showed a higher average concentrations of mycotoxins in all the products analyzed. Both AF and OTA exceeded the maximum limits allowed by international standards.
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- 2019
14. Presence of Aflatoxin M1 in Commercial Milk in Paraguay.
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ARRUA, ANDREA ALEJANDRA, ARRÚA, PABLO DAVID, MOURA-MENDES, JULIANA, CAZAL, CINTHIA, FERREIRA, FRANCISCO PAULO, GRABOWSKI, CRISTHIAN JAVIER, LOPEZ-NICORA, HORACIO DANIEL, and RIOS, DANILO FERNÁNDEZ
- Abstract
The presence of aflatoxin M
1 (AFM1 ) in milk is a public health concern because milk is a significant part of human diets worldwide. In economies where AFM1 monitoring is low or nonexistent, the possibility of AFM1 contamination might be increased. Our study was conducted to detect and quantify AFM1 in fluid milk and milk drinks of various brands, fat concentrations, packages, and heat treatments sold in the Metropolitan Area of Asunción, Paraguay. Eighty samples were collected from supermarkets in the Metropolitan Area of Asunción following a nonprobability sampling method. An enzyme-linked immunosorbent assay for AFM1 (25 to 500 ppt) was used to quantify the toxin, and results were analyzed with nonparametric methods. All samples were positive values for AFM1 (above the detection limit of 25 ng/kg); 85% of the samples had 30 to 50 ng/kg, and 15% had >500 ng/kg. No significant difference in AFM1 concentration was found based on fat concentration, heat treatment, or type of packaging of these milk products; however, significant differences were found between brands. [ABSTRACT FROM AUTHOR]- Published
- 2021
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15. Presence of Aflatoxin M1in Commercial Milk in Paraguay
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Arrúa, Andrea Alejandra, Arrúa, Pablo David, Moura-Mendes, Juliana, Cazal, Cinthia, Ferreira, Francisco Paulo, Grabowski, Cristhian Javier, Lopez-Nicora, Horacio Daniel, and Fernández Rios, Danilo
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The presence of aflatoxin M1(AFM1) in milk is a public health concern because milk is a significant part of human diets worldwide. In economies where AFM1monitoring is low or nonexistent, the possibility of AFM1contamination might be increased. Our study was conducted to detect and quantify AFM1in fluid milk and milk drinks of various brands, fat concentrations, packages, and heat treatments sold in the Metropolitan Area of Asunción, Paraguay. Eighty samples were collected from supermarkets in the Metropolitan Area of Asunción following a nonprobability sampling method. An enzyme-linked immunosorbent assay for AFM1(25 to 500 ppt) was used to quantify the toxin, and results were analyzed with nonparametric methods. All samples were positive values for AFM1(above the detection limit of 25 ng/kg); 85% of the samples had 30 to 50 ng/kg, and 15% had >500 ng/kg. No significant difference in AFM1concentration was found based on fat concentration, heat treatment, or type of packaging of these milk products; however, significant differences were found between brands.
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- 2021
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16. Ocurrencia de micotoxinas en alimentos comerciales y leche para bebé en el Área Metropolitana, Paraguay
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Cámara Paraguaya de Exportadores y Comercializadores de Cereales y Oleaginosas - CAPECO (PY), Universidad Nacional de Asunción - Facultad de Ciencias Exactas y Naturales, Arrúa Widmer, Andrea Alejandra, Arrúa Alvarenga, Pablo David, Kohli, Man Mohan, Moura-Mendes, Juliana, Cazal Martínez, Cinthia Carolina, Peralta, Inocencia Palmira, Ferreira, Francisco, Fernández Ríos, Danilo, Pereira, Mónica, Pérez Estigarribia, Pastor Enmanuel, Ulke Mayans, María Gabriela, Cámara Paraguaya de Exportadores y Comercializadores de Cereales y Oleaginosas - CAPECO (PY), Universidad Nacional de Asunción - Facultad de Ciencias Exactas y Naturales, Arrúa Widmer, Andrea Alejandra, Arrúa Alvarenga, Pablo David, Kohli, Man Mohan, Moura-Mendes, Juliana, Cazal Martínez, Cinthia Carolina, Peralta, Inocencia Palmira, Ferreira, Francisco, Fernández Ríos, Danilo, Pereira, Mónica, Pérez Estigarribia, Pastor Enmanuel, and Ulke Mayans, María Gabriela
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"Las micotoxinas poseen efectos diversos en la salud humana. Pueden afectar al sistema inmune, respiratorio, etc. o bien a órganos específicos como el hígado, riñón, pulmones. Producen intoxicaciones agudas o crónicas. Los bebés son una de las poblaciones más expuestas a los efectos de estos compuestos debido a la relación peso ingesta y a que en sus 6 primeros meses de vida consumen exclusivamente leche, que es susceptible de contaminarse con Aflatoxina M. "
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- 2018
17. Incidencia de hongos del complejo Fusarium gramínearum y acumulación de Deoxinivalenol en líneas de trigo
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Arrúa Alvarenga, Andrea Alejandra, Moura Mendes, Juliana, Cazal Martínez, Cinthia Carolina, Dujak Riquelme, Christian Eduardo, Fernández Ríos, Danilo, Oviedo de Cristaldo, Rosa María, Mohan Kohli, Man, Arrúa Alvarenga, Andrea Alejandra, Moura Mendes, Juliana, Cazal Martínez, Cinthia Carolina, Dujak Riquelme, Christian Eduardo, Fernández Ríos, Danilo, Oviedo de Cristaldo, Rosa María, and Mohan Kohli, Man
- Abstract
Los hongos del complejo Fusarium graminearum son uno de los principales problemas del cultivo del trigo a nivel mundial, debido a que ocasionan pérdidas de rendimiento y producen micotoxinas perjudiciales para la salud humana y animal. El método más eficaz para la solución de estos problemas es la prevención de la contaminación. No existen en la actualidad variedades que presenten resistencia total a estos patógenos. Es importante la identificación de variedades con potencial de resistencia en programas de mejoramiento genético. Se estudiaron in vitro 15 líneas avanzadas del Programa de Investigación en Trigo provenientes de Capitán Miranda, Itapúa; estas líneas presentaban infección natural a campo de hongos del Complejo Fusarium graminearum. La incidencia de hongos y la acumulación de Deoxinivalenol fueron evaluadas mediante análisis de varianza, comparación de medias por el test de Tukey y correlación de Spearman. Se identificaron dos líneas avanzadas de trigo con potencial de resistencia tipo III y tres con potencial de resistencia tipo V. No se encontró correlación entre la incidencia de hongos del Complejo Fusarium graminearum in vitro y la acumulación de Deoxinivalenol en las líneas estudiadas.
- Published
- 2014
18. First Report of a Leaf Blight Caused by Pyricularia pennisetigena on Cenchrus echinatus in Paraguay.
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Cazal-Martínez CC, Reyes Caballero YM, Chávez A, Pérez Estigarribia PE, Rojas A, Arrua AA, Moura-Mendes J, Lopez-Nicora HD, and Kohli MM
- Abstract
The genus Pyricularia contains several fungal species known to cause diseases on plants in the Poaceae family (Klaubauf et al. 2014; Wang et al. 2019). While sampling for P. oryzae during March-2015 and April-2018, common weed Cenchrus echinatus L. was observed with leaf lesions in and around experimental wheat fields in the departments of Canindeyú and Itapúa. C. echinatus samples from both locations displayed similar leaf lesions, varying from small light brown pinpoint to elliptical brown lesions with greyish center. Symptomatic leaves were surface disinfested and cultured on potato dextrose agar (PDA) amended with 1% gentamicin at 25°C. Two monosporic isolates were obtained, one from Itapúa (ITCeh117) and the other from Canindeyú (YCeh55). The isolates were subsequently grown on oatmeal agar (OA) and PDA under a 12-h photoperiod at 25°C and evaluated after ten days for colony diameter, sporulation, macroscopic and microscopic features. Colonies on OA reached up to 4.8 cm diameter and were light grey, whereas colonies on PDA reached up to 5.3 cm diameter and were brown with grey centers, with cottony mycelium and broad white rims. Mycelium consisted of smooth, hyaline, branched, septate hyphae 4-4.5 µm diameter. Conidiophores were erect, straight or curved, unbranched, medium brown and smooth. Conidia were solitary, pyriform, pale brown, smooth, granular, 2-septate, 32-33 × 9-10 μm; truncated with protruding hilum and varied in length from 1.0 to 1.5 μm and diameters from 2.0 to 2.2 μm. Both isolates were similar and identified as Pyricularia pennisetigena , according to morphological and morphometric characteristics (Klaubauf et al. 2014). Subsequently, genomic DNA was extracted from each isolate using the primers described in Klaubauf et al. (2014) to amplify and sequence the internal transcribed spacers (ITS), partial large subunit (LSU), partial RNA polymerase II large subunit gene (RPB1), partial actin gene (ACT), and partial calmodulin gene (CAL). Sequences from each isolate (YCeh55/ITCeh117) were deposited in GenBank with the following submission ID for ITS: MN947521/MN947526, RPB1: MN984710/MN984715, LSU: MN944829/MN944834, ACT: MN917177/MN917182, and CAL: MN984688/MN984693. Phylogenetic analysis was conducted using the software Beast v1.10.4. The results obtained from the concatenated matrix of the five loci placed these isolates in the P. pennisetigena clade. To confirm pathogenicity, each isolate was adjusted to 5×104 conidia/ml of sterile water and C. echinatus plants were sprayed with the conidial suspension for isolate YCeh55, ITCeh117 or sterile water using an oilless airbrush sprayer until runoff. The three treatments were kept in the greenhouse at 25-28°C and about 75% relative humidity under natural daylight. Each treatment included three to five inoculated plants and 10 leaves were evaluated per treatment. Symptoms were observed 8-15 days after inoculation and were similar to those originally observed in the field for both isolates, whereas the control plants remained asymptomatic. P. pennisetigena was re-isolated from the inoculated leaves fulfilling Koch's postulates. To our knowledge, this is the first report of leaf blight on C. echinatus caused by P. pennisetigena in Paraguay. The occurrence of P. pennisetigena in the region and its ability to infect economically important crops such as wheat and barley (Klaubauf et al. 2014; Reges et al., 2016, 2018) pose a potential threat to agriculture in Paraguay.
- Published
- 2021
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