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In this study, phytochemical profiling of hydro-alcoholic extract of Ammoides atlantica aerial parts has been carried out using RP-UHPLC-ESI-QTOF-MS in negative ionization mode Chemical characterization was established according to the MS and MS/MS spectra. A total of 66 chemical compounds were detected. Among these, 45 compounds were identified: hydroxycinnamic acid and derivatives (26), hydroxybenzoic acids (4), flavones (11), flavonols (3), and a lignan. Total phenolics (371.57 mg/g) and total flavonoids (41.02 mg/g) contents were also determined. Moreover, the antioxidant activity of A. atlantica extract was also studied by six methods: DPPH (IC50: 23.31 µg/mL), ABTS+ (IC50: 11.31 µg/mL), O2- DMSO alkaline (IC50: 3.19 µg/mL), ferrous ions chelating assays (IC50: 102.35 µg/mL), reducing power (A0.50: 92.70 µg/mL) and CUPRAC (A0.50: 13.56 µg/mL) assays. These results suggest that the antioxidant activity of the hydroalcoholic extract was comparable to common antioxidant additives in most of the tests, representing a good alternative.

In this study, phytochemical profiling of hydro-alcoholic extract of

In this paper, the isolation of five new guaianolides (1 – 5) and four (6 – 9) known sesquiterpenes from Ormenis mixta aerial parts is reported. The structural determination of the guaianolides was obtained by NMR spectroscopic data, as well as MS experiments. Their relative configurations were assigned by a combined quantum mechanical/NMR approach, comparing the experimental 13C/1H NMR chemical shift data and 1 J H-H homonuclear coupling constants with the related predicted values. The isolates were assayed for their anti-inflammatory potential evaluating nitric oxide release and cyclooxygenase-2 expression in J774A.1 macrophages treated with lipopolysaccharide from Escherichia coli. Our results indicated that, among the tested compounds, 1 – 3, and 7 were able to inhibit nitric oxide release, while all were able to inhibit cyclooxygenase-2 expression with different potencies.

A previously undescribed phenolic sulphate ester, potassium 34-dihydroxy-3-methoxybenzoic acid methyl ester-5-sulphate (1), along with nine known flavonoids, kaempferol-3-O-potassium sulphate-4′,7-dimethyl ether (2), kaempferol-4′,7-dimethyl ether (3), rhamnocitrin-3-O-potassium sulphate (4), rhamnocitrin (5), kaempferol (6), quercetin (7), afzelin (8), quercetin-3-O-α-l-rhamnopyranoside (9) and luteolin-3′-O-potassium sulphate (10) were isolated from the aerial parts of Tamarix balansae. Structures elucidation was performed by comprehensive 1D and 2D NMR analyses, mass spectrometry and by comparison with literature data. The antibacterial assay against Enterococcus faecalis, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli and Pseudomonas aeruginosa bacteria showed a good activity for 2, 3, 7 and 9, with MICs ranging from 62.5 to 250 μg/mL. The abilities of these compounds to scavenge the DPPH were evaluated. Compounds 1, 7, 9 and 10 exhibited a good antiradical activity potential with IC50 values ranging from 3.0 to 115.5 μg/mL, compared with ascorbic acid (IC50 7.4 μg/mL) which was used as positive control.

yellowish essential oil, having an intense and penetrating odor. Forty-one components were identified, representing 99.8% of the essential oil mainly characterized by camphor (27.4%), santolina triene (13.0%), thujone (12.9%), camphene (10.7%), and -curcumene (5.3%) (Table 1). Gas Chromatography-Mass Spectrometry. GC-MS was performed using a Shimadzu QP5050 mass selective detector and a cross-linked DB5-MS column (40 m 0.18 mm, film thickness 0.18 m). The oven temperature was programmed as isothermal at 60C for 5 min, then raised to 275C at 5C/min and held at this temperature for 5 min. Helium was used as the carrier gas at a rate of 1 ml/min. The oil (0.1 L) was introduced directly into the source of the MS via a transfer line (280C) with a split ratio of 1:50 and a linear velocity of 30.0 cm/sec. Ionization was obtained by electron impact (70 eV, source temperature 200C, resolution 1000). The present composition is quite different from the reported essential oil of Cotula coronopifolia L. leaves, which are mainly characterized by agarospirol (10.4%), and by hexacosane (31.7%) in flowers, and by 1-eicosanol (17.1%) in stems. The major constituents identified in the root oils were heptacosane (28.4%), 1-eicosanol (14.6%), octacosane (5.4%), and -amorphen (5.2%) [5].