Search

Male germ cells undergo complex developmental processes eventually producing spermatozoa through spermatogenesis, although the molecular mechanisms remain largely elusive. We have previously identified somatic cell nuclear transfer-reprogramming resistant genes (SRRGs) that are highly enriched for genes essential for spermatogenesis, although many of them remain uncharacterized in knockout (KO) mice. Here, we performed a CRISPR-based genetic screen using C57BL/6N mice for five uncharacterized SRRGs (Cox8c, Cox7b2, Tuba3a/3b, Faiml, and Gm773), together with meiosis essential gene Majin as a control. RT-qPCR analysis of mouse adult tissues revealed that the five selected SRRGs were exclusively expressed in testis. Analysis of single-cell RNA-seq datasets of adult testis revealed stage-specific expression (pre-, mid-, or post-meiotic expression) in testicular germ cells. Examination of testis morphology, histology, and sperm functions in CRISPR-injected KO adult males revealed that Cox7b2, Gm773, and Tuba3a/3b are required for the production of normal spermatozoa. Specifically, Cox7b2 KO mice produced poorly motile infertile spermatozoa, Gm773 KO mice produced motile spermatozoa with limited zona penetration abilities, and Tuba3a/3b KO mice completely lost germ cells at the early postnatal stages. Our genetic screen focusing on SRRGs efficiently identified critical genes for male germ cell development in mice, which also provides insights into human reproductive medicine.

There were 17 districts under Dhaka, Chottogram, Sylhet, Rajshahi, Khulna and Mymensingh divisions selected for this study on the basis of Turkey farming. The total 52 number of farmers interviewed to conduct this study. Turkey rearing is a profitable agricultural practice to meet the protein requirement in developing countries like Bangladesh. This is not only a source of protein but also a rich source of minerals and vitamins. The semi-intensive rearing of turkey requires low capital investment and labor cost. The main objectives of the study were to know the present status of feeding and rearing system, problems and prospects and benefit cost ratio of turkey rearing in Bangladesh. The information that collected from the respondents were age, education, main occupation, family members, training, annual income, number of turkey in each batch, name of strain, mortality rate, vaccination and medication practice, current situation of antibiotic and growth promoter use, use of footbath, use of disinfectant for spraying in inside and outside of the farm, percentage of antibiotic and feed additives withdrawal period before marketing, source of vaccine and medicine at farmer level, consultants of the farmers for vaccine and medicine specially antibiotic use, vaccination and medication cost in a batch. The average cost for production of one kg meat is BDT 231.177 and average market price of one kg meat was about BDT 452.34. Asian Australas. J. Food Saf. Secur. 2019, 3(1), 48-52

Repeat breeding (RP) is a one of the most significant problem in dairy cattle because it increased production cost of insemination, treatment, feed, labor and management and also increased calving interval with decreased milk production. This study was conducted at the different Bathan areas of Shahjadpur Upazila under Sirajgonj district. The total of 30 repeat breeder cows was selected randomly to determine their reproductive hormonal profile before and after synchronization at the selected areas and repeat breeder cows were marked by ear tag and collected breeding history of each individual cow. All experimental RP were synchronized by the administration of GnRH (day- 0), PGF2α (day-7) and Artificial Insemination (AI) with GnRH (day-9). Blood samples were collected before and after synchronization during standing heat period of estrous cycle of each cow. The total experiment was done and analyzed in the laboratory by using endocrine detection kits (Mono LelacR USA). This present study revealed that the level of luteinizing hormone (LH), follicle stimulating hormone (FSH), progesterone (P4) were significantly differed between repeat breeder cows and synchronized repeat breeder cows. These findings clearly indicated that reproductive hormonal aberrations might be one of the major causes of repeat breeding in Baghabari milk shed areas. Asian Australas. J. Biosci. Biotechnol. 2018, 3 (3), 237-240

The Geographic area of this study was 5 selected unions of Kalihati upazila in Tangail district. A total number of 50 broiler farmers were interviewed to conduct the study. The objective of the study was to know the current vaccination and medication status in the study area. The information that collected from the respondents were age, education, main occupation, family members, training, annual income, number of broiler in each batch, name of broiler strain, mortality rate, vaccination practicing rate, current situation of antibiotic and growth promoter use, use of footbath, use of disinfectant for spraying in inside and outside of the farm, percentage of antibiotic and feed withdraw rate before marketing of broiler, source of vaccine and medicine at farmer level, consultants of the farmers for vaccine and medicine specially antibiotic use, vaccination and medication cost in a batch. Commonly used vaccine and medicine list with their company name were also collected from the study area market. A correlation table was prepared to determine the relationship among some variables; education Vs age, education Vs income, income Vs no of broiler in a batch, education Vs antibiotic withdraw, income Vs antibiotic withdraw. Significant negative correlations (p

A total of nine Repeat Breeder (RB) cows consisting three cows in each of the three treatment groups were selected at farmer’s house of Nukali village of Sahjadpur Upazila under Sirajgonj district to know the feeding effect of concentrate feed on reproductive efficiency of RB cows. Concentrate feeds were given to the RB cows based on their body weight and milk production. Same management like housing, watering and quantity of green grasses and straw were ensured during the experimental period. Heat detection of cow was done by Draminski Estrous Detector (DRAMINSKI) to perform artificial insemination in standing heat period. Experimental diet samples were tested for chemical compositions in the laboratories. Data were statistically analyzed by the computer software program of SPSS. Days open or calving to conception interval were 13.00±3.51, 9.33±1.20 and 15.33±1.45 months for treatment group T1, T2 and T3, respectively. All experimental cows (100%) of treatment group one (T1) were become pregnant whereas no cows (0%) were conceived in treatment group (T3). Mean conception rate of all experimental cows was 0.56±0.18 with no significant difference. The results from the present study showed that dietary status of crossbred dairy cow can improve the reproductive efficiency like the conception rate RB cows. It’s would be concluded that diet containing optimum level of CP, Ca and P might be played a vital role for maximum conception rate of RB cows which clearly indicates the dietary intervention effect for improving the breeding efficiency of repeat breeder cows.Asian J. Med. Biol. Res. March 2018, 4(1): 137-140

The present study was designed with a view to study the effects of enrofloxacin antibiotic on ISA brown layer performance. A total of 1,80,000 laying hens (ISA brown) received and supplied enrofloxacin antibiotic in the dose of 0 mg, 10mg and 20 mg/kg BW with normal diet. Each layer was fed 120 g feed/day from 42 to 48 weeks of age. Antibiotic made significant differences in egg production (p

An experiment was conducted with 15 Black Bengal kids of both sexes (in 3 groups) were fed two different milk replacer using ingredient shotti (T1), skim milk (T2) and no milk replacer- kids with mother, termed as control group (T0) at Southern agro trade, Subarnachar, Noakhali. The average total DM intake and milk dry matter intake were not significantly differed among the treatment group. Total dry matter intake was not affected by intake of liquid milk replacer or goat milk. The CP intake were ranged from 19.97 to 20.93 g/d. Total CP intake was not significantly differed among the group but T1 group was slightly higher(0.42 g/d) than T0 and T2. The amount of protein intake (g/d) was followed NRC (1985) recommendation. DMI from concentrate (g/d), DMI from green grass (g/d) and DMI from percent live weight were not significantly differed among the treatment groups. The average daily gain was not significantly (P>0.05) differed among the treatment group. The average growth rate (57.42 – 61.20 g/d) of all kid of Black Begal goat was lower than the Angora goats (115.00-125.00 g/d) and Alpine goats (153.00-258.00 g/d). Dry matter (DM) digestibility (%) was significantly (p

A research work was undertaken to evaluate the feeding effect of tree forages on performance of growing sheep. Twenty growing sheep (in 4 groups) were fed three different tree forage diets. Tree forages Melia azardirachta, Leucaena leucocephala and Artocarpus heterophyllus were supplied in three treatments except one consisted no tree forages which was considered as control. There were significant (p

Sheep is the common name for a group of grazing mammals that may be either wild or domesticated; the domesticated varieties are amongst the most widely distributed types of domestic animal, found in nearly all countries. Bangladesh is a densely populated developing country and its economy is primarily based on agriculture. The current study was designed for isolation, identification and characterization of bacterial flora from the upper respiratory tract of sheep. Thirty (30) apparently healthy sheep were selected at the adjacent areas of Bangladesh Agricultural University (BAU) for this experiment. Swab samples were collected from nasal swabs (10), lung swabs (10) and tracheal swabs (10). All samples were subjected into inoculated on to bacteriological media (nutrient broth, nutrient agar, Salmonella-Shigella agar, MacConkey agar, blood agar, brilliant green agar). Furthermore, all of the bacterial isolates were characterized by Gram’s staining, biochemical tests (sugar fermentation tests, catalase test, coagulase test, indole test, MR-VP test), antibiotics sensitivity tests and pathogenicity tests. None of the isolated Bacillus, E. coli and Staphylococcus spp. was found to be pathogenic. Isolated Pasteurella spp. were found to be pathogenic as observed in different experimental models and showed a degree of variation in antibiotic drug sensitivity test. Ciprofloxacillin was sensitive to all of the isolated bacteria. Through the bacteria that were isolated from various organs of apparently healthy sheep is normal micro flora, however these may act as primary pathogen and may produce diseases when the sheep are immunologically suppressed due to severe stress conditions.Asian J. Med. Biol. Res. December 2015, 1(3): 677-685

Ground nut is one of the commonly used decorative nutritious relish seed around the world. Ground nut have hypocholesterolamic, hypoglycemic, hypolipidemic, anti-atherosclerotic, immune-modulatory and bacterial counting effects. In Bangladesh, limited research has so far been performed on the action of ground nut in biological system and its comparative efficacy with commercialized drugs that reduce blood cholesterol. The aim of this study was carried on the effects of PUFA on blood total cholesterol (TC), high density lipoprotein (HDL), low density lipoprotein (LDL) and triglyceride (TG) and against artificial inoculation (I/N) Staphylococcus aureus infection of experimental rat by feeding of ground nut. A total of fifteen long Evans rats (Rattus norvegicus) were used for this study. The rats were randomly divided into three equal groups (n=5) and numbered as A, B and C. Group A (control), Group B (50gm ground nut/ day/group), group C (100 gm ground nut/day/group). All groups were supplied with standard broiler pellet and fresh drinking water throughout the experimental period (January to June /2012). The blood samples were collected directly from the heart at the 1st and 60th day for biochemical test (TC, LDL, HDL and TG) and test was performed as per Memorial Diagnostic Centre, Charpara, Mymensingh. Staphylococcus aureus was cultured in nutrient broth and 100 µl of their culture was inoculated into the rats through intranasal route. Among all the treated groups, the rats of group C exhibited the lowest TC value, TG and LDL and reduced blood cholesterol significantly than (control group A) followed by group B. In bacteriological examinations, it was found that the number of bacterial colony lowest in group C in comparison with the group of A and B. From the present experiment, it can be assumed that PUFA has significant effect on blood lipid profile and against bacterial infection.Asian J. Med. Biol. Res. June 2015, 1(2): 235-243