1. The glutathione transferase of Nicotiana benthamiana NbGSTU4 plays a role in regulating the early replication of Bamboo mosaic virus.
- Author
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Chen IH, Chiu MH, Cheng SF, Hsu YH, and Tsai CH
- Subjects
- 3' Untranslated Regions genetics, Blotting, Western, Cross-Linking Reagents pharmacology, Gene Knockdown Techniques, Glutathione pharmacology, Mosaic Viruses drug effects, Mosaic Viruses radiation effects, Oxidation-Reduction drug effects, Oxidation-Reduction radiation effects, RNA, Viral biosynthesis, RNA, Viral genetics, RNA-Dependent RNA Polymerase metabolism, Real-Time Polymerase Chain Reaction, Nicotiana drug effects, Nicotiana radiation effects, Ultraviolet Rays, Glutathione Transferase metabolism, Mosaic Viruses physiology, Plant Proteins metabolism, Nicotiana enzymology, Nicotiana virology, Virus Replication drug effects, Virus Replication radiation effects
- Abstract
Bamboo mosaic virus (BaMV) is a single-stranded positive-sense RNA virus. One of the plant glutathione S-transferase (GST) genes, NbGSTU4, responds as an upregulated gene in Nicotiana benthamiana post BaMV infection. In order to identify the role of NbGSTU4 in BaMV infection, the expression of NbGSTU4 was knocked down using a virus-induced gene silencing technique or was transiently expressed in N. benthamiana in BaMV inoculation. The results show a significant decrease in BaMV RNA accumulation when the expression level of NbGSTU4 is reduced; whereas the viral RNA accumulation increases when NbGSTU4 is transiently expressed. Furthermore, this study identified that the involvement of NbGSTU4 in viral RNA accumulation occurs by its participation in the viral early replication step. The findings show that the NbGSTU4 protein expressed from Escherichia coli can interact with the 3' untranslated region (UTR) of the BaMV RNA in vitro in the presence of glutathione (GSH). The addition of GSH in the in vitro replication assay shows an enhancement of minus-strand but not plus-strand RNA synthesis. The results suggest that the plant GST protein plays a role in binding viral RNA and delivering GSH to the replication complex to create a reduced condition for BaMV minus-strand RNA synthesis., (© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust.)
- Published
- 2013
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