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3 results on '"Morrissey-Scoot, James"'

1. CD24 tracks divergent pluripotent states in mouse and human cells

Author

Shakiba, Nika, White, Carl A, Lipsitz, Yonatan Y, Yachie-Kinoshita, Ayako, Tonge, Peter D, Hussein, Samer M I, Puri, Mira C, Elbaz, Judith, Morrissey-Scoot, James, Li, Mira, Munoz Peralta, Javier, Benevento, Marco, Rogers, Ian M, Hanna, Jacob H, Heck, Albert J R, Wollscheid, Bernd, Nagy, Andras, Zandstra, Peter W, Biomolecular Mass Spectrometry and Proteomics, Sub Biomol.Mass Spectrometry & Proteom., Sub Biomol.Mass Spect. and Proteomics, Biomolecular Mass Spectrometry and Proteomics, Sub Biomol.Mass Spectrometry & Proteom., Sub Biomol.Mass Spect. and Proteomics, Ontario Government, Canadian Institutes of Health Research, and NSERC Vanier Canada Graduate Scholarship

Subjects
Cell biology, Human Embryonic Stem Cells, Induced Pluripotent Stem Cells, General Physics and Astronomy, Germ layer, Embryoid body, Biology, General Biochemistry, Genetics and Molecular Biology, Article, 03 medical and health sciences, Mice, 0302 clinical medicine, Developmental biology, Animals, Humans, Induced pluripotent stem cell, 030304 developmental biology, Genetics, 0303 health sciences, Multidisciplinary, Stem Cells, CD24 Antigen, Mouse Embryonic Stem Cells, General Chemistry, Cellular Reprogramming, Embryonic stem cell, Biological sciences, Epiblast, Stem cell, Reprogramming, 030217 neurology & neurosurgery, Germ Layers
Abstract

Reprogramming is a dynamic process that can result in multiple pluripotent cell types emerging from divergent paths. Cell surface protein expression is a particularly desirable tool to categorize reprogramming and pluripotency as it enables robust quantification and enrichment of live cells. Here we use cell surface proteomics to interrogate mouse cell reprogramming dynamics and discover CD24 as a marker that tracks the emergence of reprogramming-responsive cells, while enabling the analysis and enrichment of transgene-dependent (F-class) and -independent (traditional) induced pluripotent stem cells (iPSCs) at later stages. Furthermore, CD24 can be used to delineate epiblast stem cells (EpiSCs) from embryonic stem cells (ESCs) in mouse pluripotent culture. Importantly, regulated CD24 expression is conserved in human pluripotent stem cells (PSCs), tracking the conversion of human ESCs to more naive-like PSC states. Thus, CD24 is a conserved marker for tracking divergent states in both reprogramming and standard pluripotent culture., Nature Communications, 6, ISSN:2041-1723

Published
2015
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2. CD24 tracks divergent pluripotent states in mouse and human cells

Author

Biomolecular Mass Spectrometry and Proteomics, Sub Biomol.Mass Spectrometry & Proteom., Sub Biomol.Mass Spect. and Proteomics, Shakiba, Nika, White, Carl A, Lipsitz, Yonatan Y, Yachie-Kinoshita, Ayako, Tonge, Peter D, Hussein, Samer M I, Puri, Mira C, Elbaz, Judith, Morrissey-Scoot, James, Li, Mira, Munoz Peralta, Javier, Benevento, Marco, Rogers, Ian M, Hanna, Jacob H, Heck, Albert J R, Wollscheid, Bernd, Nagy, Andras, Zandstra, Peter W, Biomolecular Mass Spectrometry and Proteomics, Sub Biomol.Mass Spectrometry & Proteom., Sub Biomol.Mass Spect. and Proteomics, Shakiba, Nika, White, Carl A, Lipsitz, Yonatan Y, Yachie-Kinoshita, Ayako, Tonge, Peter D, Hussein, Samer M I, Puri, Mira C, Elbaz, Judith, Morrissey-Scoot, James, Li, Mira, Munoz Peralta, Javier, Benevento, Marco, Rogers, Ian M, Hanna, Jacob H, Heck, Albert J R, Wollscheid, Bernd, Nagy, Andras, and Zandstra, Peter W

Published
2015

3. CD24 tracks divergent pluripotent states in mouse and human cells.

Author

Shakiba N, White CA, Lipsitz YY, Yachie-Kinoshita A, Tonge PD, Hussein SMI, Puri MC, Elbaz J, Morrissey-Scoot J, Li M, Munoz J, Benevento M, Rogers IM, Hanna JH, Heck AJR, Wollscheid B, Nagy A, and Zandstra PW

Subjects
Animals, Germ Layers cytology, Human Embryonic Stem Cells cytology, Humans, Induced Pluripotent Stem Cells cytology, Mice, Mouse Embryonic Stem Cells cytology, Stem Cells cytology, Stem Cells metabolism, CD24 Antigen metabolism, Cellular Reprogramming, Human Embryonic Stem Cells metabolism, Induced Pluripotent Stem Cells metabolism, Mouse Embryonic Stem Cells metabolism
Abstract

Reprogramming is a dynamic process that can result in multiple pluripotent cell types emerging from divergent paths. Cell surface protein expression is a particularly desirable tool to categorize reprogramming and pluripotency as it enables robust quantification and enrichment of live cells. Here we use cell surface proteomics to interrogate mouse cell reprogramming dynamics and discover CD24 as a marker that tracks the emergence of reprogramming-responsive cells, while enabling the analysis and enrichment of transgene-dependent (F-class) and -independent (traditional) induced pluripotent stem cells (iPSCs) at later stages. Furthermore, CD24 can be used to delineate epiblast stem cells (EpiSCs) from embryonic stem cells (ESCs) in mouse pluripotent culture. Importantly, regulated CD24 expression is conserved in human pluripotent stem cells (PSCs), tracking the conversion of human ESCs to more naive-like PSC states. Thus, CD24 is a conserved marker for tracking divergent states in both reprogramming and standard pluripotent culture.

Published
2015
Full Text
View/download PDF

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