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354 results on '"Morpurgo, M."'

1. Development of a Nanoparticle-Based Approach for the Blood–Brain Barrier Passage in a Murine Model of Amyotrophic Lateral Sclerosis

Author

Violatto, M, Pasetto, L, Casarin, E, Tondello, C, Schiavon, E, Talamini, L, Marchini, G, Cagnotto, A, Morelli, A, Lanno, A, Passoni, A, Bigini, P, Morpurgo, M, Bonetto, V, Violatto M. B., Pasetto L., Casarin E., Tondello C., Schiavon E., Talamini L., Marchini G., Cagnotto A., Morelli A., Lanno A., Passoni A., Bigini P., Morpurgo M., Bonetto V., Violatto, M, Pasetto, L, Casarin, E, Tondello, C, Schiavon, E, Talamini, L, Marchini, G, Cagnotto, A, Morelli, A, Lanno, A, Passoni, A, Bigini, P, Morpurgo, M, Bonetto, V, Violatto M. B., Pasetto L., Casarin E., Tondello C., Schiavon E., Talamini L., Marchini G., Cagnotto A., Morelli A., Lanno A., Passoni A., Bigini P., Morpurgo M., and Bonetto V.

Abstract

The development of nanoparticles (NPs) to enable the passage of drugs across blood–brain barrier (BBB) represents one of the main challenges in neuropharmacology. In recent years, NPs that are able to transport drugs and interact with brain endothelial cells have been tested. Here, we investigated whether the functionalization of avidin-nucleic-acid-nanoassembly (ANANAS) with apolipoprotein E (ApoE) would allow BBB passage in the SOD1G93A mouse model of amyotrophic lateral sclerosis. Our results demonstrated that ANANAS was able to transiently cross BBB to reach the central nervous system (CNS), and ApoE did not enhance this property. Next, we investigated if ANANAS could improve CNS drug delivery. To this aim, the steroid dexamethasone was covalently linked to ANANAS through an acid-reversible hydrazone bond. Our data showed that the steroid levels in CNS tissues of SOD1G93A mice treated with nanoformulation were below the detection limit. This result demonstrates that the passage of BBB is not sufficient to guarantee the release of the cargo in CNS and that a different strategy for drug tethering should be devised. The present study furthermore highlights that NPs can be useful in improving the passage through biological barriers but may limit the interaction of the therapeutic compound with the specific target.

Published
2022

2. Detection of a fluorescent-labeled avidin-nucleic acid nanoassembly by confocal laser endomicroscopy in the microvasculature of chronically inflamed intestinal mucosa

Author

Buda A, Facchin S, Dassie E, Casarin E, Jepson MA, Neumann H, Hatem G, Realdon S, D’Incà R, Sturniolo GC, and Morpurgo M

Subjects
Medicine (General), R5-920
Abstract

Andrea Buda,1,* Sonia Facchin,1,* Elisa Dassie,2 Elisabetta Casarin,3 Mark A Jepson,4 Helmut Neumann,5 Giorgia Hatem,1 Stefano Realdon,6 Renata D’Incà,1 Giacomo Carlo Sturniolo,1 Margherita Morpurgo3 1Department of Surgical, Oncological, and Gastroenterological Sciences, University of Padova, 2Department of Molecular Medicine, University of Padova, Padova, Italy; 3Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy; 4School of Biochemistry and Wolfson Bioimaging Facility, University of Bristol, Bristol, UK; 5Ludwig Demlig Endoscopic Center of Excellence, ESGE Endoscopy Training Center, University of Erlangen-Nuremberg, Erlangen, Germany; 6Veneto Institute of Oncology IOV-IRCCS, Padova, Italy *These authors contributed equally to this work Abstract: Inflammatory bowel diseases are chronic gastrointestinal pathologies causing great discomfort in both children and adults. The pathogenesis of inflammatory bowel diseases is not yet fully understood and their diagnosis and treatment are often challenging. Nanoparticle-based strategies have been tested in local drug delivery to the inflamed colon. Here, we have investigated the use of the novel avidin-nucleic acid nanoassembly (ANANAS) platform as a potential diagnostic carrier in an experimental model of inflammatory bowel diseases. Fluorescent-labeled ANANAS nanoparticles were administered to mice with chemically induced chronic inflammation of the large intestine. Localization of mucosal nanoparticles was assessed in vivo by dual-band confocal laser endomicroscopy. This technique enables characterization of the mucosal microvasculature and crypt architecture at subcellular resolution. Intravascular nanoparticle distribution was observed in the inflamed mucosa but not in healthy controls, demonstrating the utility of the combination of ANANAS and confocal laser endomicroscopy for highlighting intestinal inflammatory conditions. The specific localization of ANANAS in inflamed tissues supports the potential of this platform as a targeted carrier for bioactive moieties in the treatment of inflammatory bowel disease. Keywords: confocal laser endomicroscopy, inflammatory bowel disease, diagnostics, dextran sodium sulfate, avidin-nucleic acid nanoassembly, fluorescent nanoparticles, ulcerative colitis

Published
2015

6. The mode of dexamethasone decoration influences avidin-nucleic-acid-nano-assembly organ biodistribution and in vivo drug persistence

Author

Ongaro, A, Violatto, M, Casarin, E, Pellerani, I, Marchini, G, Ribaudo, G, Salmona, M, Carbone, M, Passoni, A, Gnodi, E, Schiavon, E, Mattarei, A, Barisani, D, Invernizzi, P, Bigini, P, Morpurgo, M, Violatto, MB, Ongaro, A, Violatto, M, Casarin, E, Pellerani, I, Marchini, G, Ribaudo, G, Salmona, M, Carbone, M, Passoni, A, Gnodi, E, Schiavon, E, Mattarei, A, Barisani, D, Invernizzi, P, Bigini, P, Morpurgo, M, and Violatto, MB

Abstract

Avidin-Nucleic-Acid-NanoASsemblies (ANANAS) possess natural tropism for the liver and, when loaded with dexamethasone, reduce clinical progression in an autoimmune hepatitis murine model. Here, we investigated the linker chemistry (hydrazide-hydrazone, Hz-Hz, or carbamate hydrazide-hydrazone, Cb-Hz bond) and length (long, 5 kDa PEG, or short, 5-6 carbons) in biotin-dexamethasone conjugates used for nanoparticle decoration through in vitro and in vivo studies. All four newly synthesized conjugates released the drug at acidic pH only. In vitro, the Hz-Hz and the PEG derivatives were less stable than the Cb-Hz and the short chain ones, respectively. Once injected in healthy mice, dexamethasone location in the PEGylated ANANAS outer layer favors liver penetration and resident macrophages uptake, while drug Hz-Hz, but not Cb-Hz, short spacing prolongs drug availability. In conclusion, the tight modulation of ANANAS decoration can significantly influence the host interaction, paving the way for the development of steroid nanoformulations suitable for different pharmacokinetic profiles.

Published
2022

14. Dexamethasone Conjugation to Biodegradable Avidin-Nucleic-Acid-Nano-Assemblies Promotes Selective Liver Targeting and Improves Therapeutic Efficacy in an Autoimmune Hepatitis Murine Model

Author

Violatto, M, Casarin, E, Talamini, L, Russo, L, Baldan, S, Tondello, C, Messmer, M, Hintermann, E, Rossi, A, Passoni, A, Bagnati, R, Biffi, S, Toffanin, C, Gimondi, S, Fumagalli, S, De Simoni, M, Barisani, D, Salmona, M, Christen, U, Invernizzi, P, Bigini, P, Morpurgo, M, Violatto, MB, FUMAGALLI, STEFANO, De Simoni, MG, Violatto, M, Casarin, E, Talamini, L, Russo, L, Baldan, S, Tondello, C, Messmer, M, Hintermann, E, Rossi, A, Passoni, A, Bagnati, R, Biffi, S, Toffanin, C, Gimondi, S, Fumagalli, S, De Simoni, M, Barisani, D, Salmona, M, Christen, U, Invernizzi, P, Bigini, P, Morpurgo, M, Violatto, MB, FUMAGALLI, STEFANO, and De Simoni, MG

Abstract

Steroids are the standard therapy for autoimmune hepatitis (AIH) but the long-lasting administration is hampered by severe side effects. Methods to improve the tropism of the drug toward the liver are therefore required. Among them, conjugation to nanoparticles represents one possible strategy. In this study, we exploited the natural liver tropism of Avidin-Nucleic-Acid-Nano-Assemblies (ANANAS) to carry dexamethasone selectively to the liver in an AIH animal model. An acid-labile biotin-hydrazone linker was developed for reversible dexamethasone loading onto ANANAS. The biodistribution, pharmacokinetics and efficacy of free and ANANAS-linked dexamethasone (ANANAS-Hz-Dex) in healthy and AIH mice were investigated upon intraperitoneal administration. In ANANAS-treated animals, the free drug was detected only in the liver. Super-resolution microscopy showed that nanoparticles segregate inside lysosomes of liver immunocompetent cells, mainly involved in AIH progression. In agreement with these observational results, chronic low-dose treatment with ANANAS-Hz-Dex reduced the expression of liver inflammation markers and, in contrast to the free drug, also the levels of circulating AIH-specific autoantibodies. These data suggest that the ANANAS carrier attenuates AIH-related liver damage without drug accumulation in off-site tissues. The safety and biodegradability of the ANANAS carrier make this formulation a promising tool for the treatment of autoimmune liver disorders.

Published
2019

15. Advances in technology for high-energy subnuclear physics Contribution of the LAA project

Author

Acosta, D., Alberty, J., Alsford, J., Alvisi, C., Ambrosi, G., Anghinolfi, F., Anselmo, F., Anzivino, G., Arneodo, M., Arnold, R., Arzarello, F., Aspell, P., Barberio, L. E., Bari, G., Barillari, T., Basile, M., Battiston, R., Baudoin-Bijst, C., Becker, U., Bellagamba, L., Bénot, M., Benvenuto, P., Berbiers, J., Berdugo, J., Bergsma, F., Bertin, R., Bingefors, N., Bisello, D., Bock, R. K., Boscherini, D., Bosteels, M., Bouclier, R., Bramhall, M., Bruni, G., Buontempo, S., Calôba, L., Campbell, M., Caputi, L., Romeo, G. Cara, Caria, M., Casaccia, R., Castro, H., Ceresara, S., Chapuis, J. M., Charpak, G., Chesi, E., Chiarini, M., Christiansen, J., Christofel, E., Cifarelli, L., Cindolo, F., Colavita, F., Coninckx, F., Contin, A., Costa, M., Crotty, I., D’Alí, G., D’Ambrosio, C., D’Auria, S., Dardo, M., Del Papa, C., Della Gatta, G., De Pasquale, S., De Salvo, R., De Seixas, J. M., Destruel, P., de Witt, J., Di Rosa, O., Dorfan, D., Duchovni, E., Dupont, J., Dupraz, J., Egger, J., Ekelof, T., Enz, C. C., Ereditato, A., Ermoline, Y., Fabre, J. P., Feraudet, P., Ferrari, R., Fiori, F., Ford, P., Frasconi, F., Fraternali, M., French, M., Fuchs, M., Fumagalli, G., Gabathuler, K., Galvez, J., Gaudaen, J., Gildemeister, O., Giomataris, Y., Girod, J. P., Giusti, P., Goebel, K., Goiugas, A., Grinnel, C., Güsten, H., Guyonnet, J. L., Gys, T., Hartjes, F., Hazifotiadu, D., Heijne, E., Henkes, T., Henriques, A. M., Hourican, M., Iacobucci, G., Iuvino, G., Jarron, P., Jenni, P., Jobez, J. P., Joram, C., Kluge, W., Krisher, W., Krummenacher, F., Kuzucu, A., Laakso, I., Labbe, J. C., La Commare, G., Larsen, H., Laurenti, G., Lee, T. D., Letheren, M., Leutz, H., Levi, G., Levinson, L., Lin, Q., Linssen, L., Lisowski, B., Litke, A., Livan, M., Ljuslin, C., Lone, L., Maccarrone, G., Maio, A., Mapelli, L., Marchioro, A., Margotti, A., Marino, M., Massam, T., Matsuda, T., Matsuura, T., Mattern, D., Meddeler, G., Meier, K. H., Meng, R., Mikenberg, G., Million, G., Mondardini, M. R., Mörk, G., Morpurgo, M., Musso, B., Nania, R., Nemoz, C., Newett, S., Oliva, A., Olsen, A., Ong, B., O’Shea, V., Ozdes, N., Paar, H. P., Palermo, L., Cernicchiaro, S. Palermo, Palmonari, F., Passardi, G., Pastore, F., Pelfer, P., Pereira, M., Peroni, C., Perotto, E., Peskov, V., Piedigrossi, D., Pilastrini, R., Pitzl, D., Poggioli, L., Pol, M. E., Qian, S., Racz, A., Rivera, F., Rose-Dulcina, L., Ruf, T., Sadrozinski, H., Salgne, R., Sandoval, A., Sannier, G., Santiard, J. C., Sartorelli, G., Sartori, P., Sauli, F., Scheel, C., Schioppa, M., Schipper, J., Schönbacher, H., Scigocki, D., Scioni, M., Seguinot, J., Seiden, A., Seidl, W., Seixas, J. M., Sharp, P., Sigrist, A., Simon, A., Simonet, G., Sivertz, M., Smith, K., Sonderegger, P., Souza, M. N., Spencer, E., Sportelli, L., Staiano, K., Susinno, G. C., Tailhardat, S., Taufer, M., Taufer, M., Tavlet, M., Terraneo, A. E., Thomé, Z. D., Timellini, R., Tischhauser, J., Tocqueville, J., Valencic, V., Van Eijk, B., Vanstraelen, G., Vercesi, V., Votano, L., Wenninger, H., Werner, C., Wigmans, R., Williams, C., Ypsilantis, G., Zaganidis, N., Zichichi, A., and Zografos, K.

Published
1990
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27. Distribuzione dei gamberi d’acqua dolce in Italia

Author

Morpurgo, M., Aquiloni, L., Bertocchi, S., Brusconi, S., Tricarico, E., and Gherardi, F.

Subjects
freshwater crayfish, distribution, indigenous species, allochthonous species, Italy
Published
2010

28. Süsswasserkrebse In Italien

Author

Gherardi, F., Aquiloni, L., Tricarico, E., and Morpurgo, M.

Subjects
crayfish, Italy, distribution, alien species, indigenous species
Published
2008

37. Comments on the guidelines of the European Society of Cardiology Task Force on pulmonary embolism

Author

Zonzin, P., Agnelli, G., Casazza, F., Favretto, G., Giuntini, C., Morpurgo, M., and Vizza, Carmine Dario

Subjects
Vena Cava Filters, Critical Illness, Pregnancy Complications, Cardiovascular, Embolectomy, Pulmonary Artery, Sensitivity and Specificity, embolism, Postoperative Complications, Fibrinolytic Agents, Pregnancy, Risk Factors, Thromboembolism, Humans, Thrombolytic Therapy, Prospective Studies, guidelines, Radionuclide Imaging, Probability, Heparin, Angiography, Anticoagulants, Puerperal Disorders, Heparin, Low-Molecular-Weight, Echocardiography, cardiology, Practice Guidelines as Topic, Female, Pulmonary Embolism, Tomography, X-Ray Computed
Published
2001

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