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27 results on '"Morona, Judy K."'

6. Streptococcus pneumoniae capsule biosynthesis protein CpsB is a novel manganese-dependent phosphotyrosine-protein phosphatase

Author

Morona, Judy K., Morona, Renato, Miller, David C., and Paton, James C.

Subjects
Tyrosine metabolism -- Physiological aspects, Enzymes -- Synthesis, Bacterial proteins -- Physiological aspects, Structure-activity relationships (Biochemistry) -- Analysis, Biological sciences
Abstract

The capsule biosynthesis protein CpsB in Streptococcus pneumoniae is a member of the polymerase and histidinol phosphatase family of phosphoesterases. Data suggest that CpsB requires manganese for optimal activity and mutation of the histidine residues inactivates the enzyme.

Published
2002

7. Comparative genetics of capsular polysaccharide biosynthesis in Streptococcus pneumoniae types belonging to serogroup 19

Author

Morona, Judy K., Morona, Renato, and Paton, James C.

Subjects
Molecular microbiology -- Research, Biosynthesis -- Research, Polysaccharides -- Research, Streptococcus pneumoniae -- Research, Biological sciences
Abstract

The article discusses Streptococcus pneumoniae genetic mechanisms to produce diversity in capsular polysaccharide structure. The results of DNA sequence analysis are presented.

Published
1999

8. Molecular and genetic characterization of the capsule biosynthesis locus of Streptococcus pneumoniae type 23F

Author

Morona, Judy K., Miller, David C., Coffey, Tracey J., Vindurampulle, Christofer J., Spratt, Brian G., Morona, Renato, and Paton, James C.

Subjects
Polymerase chain reaction -- Research, Streptococcus pneumoniae -- Genetic aspects, Biosynthesis -- Research, Nucleotide sequence -- Research, Polysaccharides -- Research, Gene amplification -- Research, Cloning -- Research, Biological sciences
Abstract

The region of the Streptococcus pneumoniae type 23F capsule locus between cps23fB and cps23fL has been amplified and cloned using long-range polymerase chain reaction. This section is 13 kb in size and consists of 12 new protein products. cps23f consists of 18 genes found in the pneumococcal chromosome between dexB and aliA. Putative functions have been assigned to the majority of cps23f genes based on similarity with known proteins and with reference to the structure of the type 23F repeat unit.

Published
1999

9. Molecular and genetic characterization of the capsule biosynthesis locus of streptococcus penumoniae type 19

Author

Morona, Judy K., Morona, Renato, and Paton, James C.

Subjects
Streptococcus pneumoniae -- Research, Biosynthesis -- Usage, Biological sciences
Abstract

The pneumococcus streptococcus pneumoniae causes invasive disease and control is hindered by antibiotic-resistant strains. S. pneumoniae has 90 recognized serotypes each producing capsular polysaccharide (CPS). The isolation and characterization of a part of cps19b encoding type-specific functions has been undertaken. It is suggested that further genes are required for 19B CPS biosynthesis.

Published
1997

10. Isolation, characterization and nucleotide sequence of IS1202, an insertion sequence of Streptococcus pneumoniae

Author

Morona, Judy K., Guidolin, Angelo, Morona, Renato, Hansman, David, and Paton, James C.

Subjects
Streptococcus pneumoniae -- Genetic aspects, Insertion elements, DNA -- Analysis, Biological sciences
Abstract

A comparative hybridization process is used to isolate a segment of pneumococcal DNA found in the Streptococcus pneumoniae type 19F strain SSZ. The segment shows all the characteristics of a bacterial insertion sequence. A copy of the element IS1202 closely resembles the type 19F polysaccharide synthesis locus in strain SSZ and is located 1,009 bp downstream of the dexB gene.

Published
1994

15. Mutational analysis of the carboxy-terminal (YGX)(sub)4 repeat domain of CpsD, an autophosphorylating tyrosine kinase required for capsule biosynthesis in Streptococcus pneumoniae

Author

Morona, Judy K., Morona, renato, Miller, David C., and Paton, James C.

Subjects
Microbiological synthesis -- Genetic aspects, Microbial mutation -- Analysis, Polysaccharides -- Physiological aspects, Structure-activity relationships (Biochemistry) -- Analysis, Heredity, Biological sciences
Abstract

Mutational analysis indicate that a CpsD truncation mutant containing no carboxy terminal (YGX)(sub)4 repeat domain is similar to a strain in which the entire cpsD gene is deleted, suggesting that the repeat domain is essential for CpsD activity and capsular polysaccharide production. Furthermore, results reveal thatthe tyrosine residues of the repeat domain are required for CpsD normal function.

Published
2003

27. Regions of the cloned Vibrio cholerae rfbgenes needed to determine the Ogawa form of the O-antigen

Author

Morona, Renato, Matthews, Mary S., Morona, Judy K., and BrownBrownBrown, Melissa H.

Abstract

The O-antigen of the lipopolysaccharides of Vibrio cholerae01 can exist in two forms termed Inaba and Ogawa. We used a complementation system to demonstrate that the Ogawa phenotype is dominant over the Inaba phenotype. By using a set of deletions affecting the Ogawa rfbgenes, we identified two regions which are needed to confer the Ogawa phenotype. In vitro mutagenesis of the cloned Ogawa rfbgenes resulted in the isolation of variants with the Inaba phenotype. The results are interpreted with respect to previous studies demonstrating interconversion between the two forms of the V. choleraeO-antigen.

Published
1990
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