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21 results on '"Moritoh, Satoru"'

1. Synaptic pruning through glial synapse engulfment upon motor learning

Author

Morizawa, Yosuke M., Matsumoto, Mami, Nakashima, Yuka, Endo, Narumi, Aida, Tomomi, Ishikane, Hiroshi, Beppu, Kaoru, Moritoh, Satoru, Inada, Hitoshi, Osumi, Noriko, Shigetomi, Eiji, Koizumi, Schuichi, Yang, Guang, Hirai, Hirokazu, Tanaka, Kohichi, Tanaka, Kenji F., Ohno, Nobuhiko, Fukazawa, Yugo, and Matsui, Ko

Published
2022
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3. Optogenetic stimulus-triggered acquisition of seizure resistance

Author

Shimoda, Yoshiteru, primary, Beppu, Kaoru, additional, Ikoma, Yoko, additional, Morizawa, Yosuke M., additional, Zuguchi, Satoshi, additional, Hino, Utaro, additional, Yano, Ryutaro, additional, Sugiura, Yuki, additional, Moritoh, Satoru, additional, Fukazawa, Yugo, additional, Suematsu, Makoto, additional, Mushiake, Hajime, additional, Nakasato, Nobukazu, additional, Iwasaki, Masaki, additional, Tanaka, Kenji F., additional, Tominaga, Teiji, additional, and Matsui, Ko, additional

Published
2022
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4. Involvement of the mitochondrial protein translocator component tim50 in growth, cell proliferation and the modulation of respiration in Drosophila

Author

Sugiyama, Shin, Moritoh, Satoru, Furukawa, Yoshimi, Mizuno, Tomohiko, Lim, Young-Mi, Tsuda, Leo, and Nishida, Yasuyoshi

Subjects
Drosophila -- Physiological aspects, Drosophila -- Growth, Drosophila -- Genetic aspects, Mitochondria -- Chemical properties, Mitochondria -- Genetic aspects, Mitochondrial membranes -- Chemical properties, Membrane proteins -- Physiological aspects, Biological control systems -- Chemical properties, Company growth, Biological sciences
Abstract

Allelic mutants exhibiting growth defects in Drosophila were isolated. Molecular cloning identified the responsible gene as a budding yeast Tim50 ortholog, and thus it was named tiny tim 50 (ttm50). The weak allele ([ttm50.sup.Gp99]) produced small flies due to reduced cell size and number, and growth terminated at the larval stage in the strong alleles ([ttm50.sup.IE1] and [ttm50.sup.IE2]). Twin-spot analysis showed fewer cells in [ttm50.sup.Gp99] clones, whereas [ttm50.sup.IE1] clones did not proliferate, suggesting that the gene has an essential cellular function. Tim50 is known to maintain mitochondrial membrane potential (MMP) while facilitating innermembrane protein transport. We found that tagged Ttm50 also localized to mitochondria and that mitochondrial morphology and MMP were affected in mutants, indicating that mitochondrial dysfunction causes the developmental phenotype. Conversely, ttm50 overexpression increased MMP and apoptosis. Co-expression of p35 suppressed this apoptosis, resulting in cell overproliferation. Interestingly, ttm50 transcription was tissue specific, corresponding to elevated MMP in the larval midgut, which was decreased in the mutant. The correlation of ttm50 expression levels with differences in MMP match its proposed role in mitochondrial permeability barrier maintenance. Thus a mitochondrial protein translocase component can play active roles in regulating metabolic levels, possibly for modulation of physiological function or growth in development.

Published
2007

9. Bilberry extract administration prevents retinal ganglion cell death in mice via the regulation of chaperone molecules under conditions of endoplasmic reticulum stress

Author

Nakamura,Orie, Moritoh,Satoru, Sato,Kota, Maekawa,Shigeto, Murayama,Namie, Himori,Noriko, Omodaka,Kazuko, Sogon,Tetsuya, Nakazawa,Toru, Nakamura,Orie, Moritoh,Satoru, Sato,Kota, Maekawa,Shigeto, Murayama,Namie, Himori,Noriko, Omodaka,Kazuko, Sogon,Tetsuya, and Nakazawa,Toru

Abstract

Orie Nakamura,1 Satoru Moritoh,1,2 Kota Sato,1,3 Shigeto Maekawa,1 Namie Murayama,1 Noriko Himori,1 Kazuko Omodaka,1,3 Tetsuya Sogon,4 Toru Nakazawa1–3,5 1Department of Ophthalmology, Tohoku University Graduate School of Medicine, Miyagi, Japan; 2Department of Retinal Disease Control, Tohoku University Graduate School of Medicine, Miyagi, Japan; 3Department of Ophthalmic Imaging and Information Analytics, Tohoku University Graduate School of Medicine, Miyagi, Japan; 4R&D Department, Wakasa Seikatsu Co., Ltd., Kyoto, Japan; 5Department of Advanced Ophthalmic Medicine, Tohoku University Graduate School of Medicine, Miyagi, Japan Purpose: To investigate the effect of bilberry extract anthocyanins on retinal ganglion cell (RGC) survival after optic nerve crush. Additionally, to determine details of the mechanism of the neuroprotective effect of bilberry extract anthocyanins and the involvement of endoplasmic reticulum stress suppression in the mouse retina.Materials and methods: Anthocyanins in bilberry extract (100 mg/kg/day or 500 mg/kg/day) were administrated orally to C57BL/6J mice. The expression levels of various molecular chaperones were assessed with quantitative reverse-transcription polymerase chain reaction, Western blotting, and immunohistochemistry. RGC survival was evaluated by measuring the gene expression of RGC markers and counting retrogradely labeled RGCs after optic nerve crush.Results: The protein levels of Grp78 and Grp94 increased significantly in mice after bilberry extract administration. Increased Grp78 and Grp94 levels were detected in the inner nuclear layer and ganglion cell layer of the retina, surrounding the RGCs. Gene expression of Chop, Bax, and Atf4 increased in mice after optic nerve crush and decreased significantly after oral bilberry extract administration. RGC survival after nerve crush also increased with bilberry extract administration.Conclusion: These results indicate that oral bilberry extract administration

Published
2017

10. Additional file 1: Figure S1. of Topical ocular dexamethasone decreases intraocular pressure and body weight in rats

Author

Sato, Kota, Nishiguchi, Koji, Kazuichi Maruyama, Moritoh, Satoru, Fujita, Kosuke, Ikuta, Yoshikazu, Kasai, Hitoshi, and Nakazawa, Toru

Subjects
genetic structures, sense organs, eye diseases
Abstract

IOP reduction in untreated left eye after topical dexamethasone treatment in rat. Topical ocular vehicle or DEX was administered 3 times daily for up to 4 weeks in the right eye. IOP measurements of untreated contralateral left eye from vehicle-treated and 0.1% dexamethasone-treated rats are shown. Values represent the mean Âą standard deviation (N = 12). * P < 0.05, *** P < 0.001, unpaired t-test. Figure S2. No alteration of the retinal histology or loss of RGCs observed following topical ocular treatment with dexamethasone in the rats. Retinal histological sections were stained by H&E. GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer. Scale bars=50 Îźm. (DOCX 119 kb)

Published
2016
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11. Endogenous arginine vasopressin–positive retinal cells in arginine vasopressin–eGFP transgenic rats identified by immunohistochemistry and reverse transcriptase–polymerase chain reaction

Author

Moritoh, Satoru, Sato, Kaori, Okada, Yasunobu, and Koizumi, Amane

Subjects
Male, Retinal Ganglion Cells, endocrine system, Receptors, Vasopressin, urogenital system, Reverse Transcriptase Polymerase Chain Reaction, Green Fluorescent Proteins, Gene Expression, Immunohistochemistry, Rats, Arginine Vasopressin, nervous system, Paracrine Communication, Animals, Vasoconstrictor Agents, sense organs, RNA, Messenger, Rats, Transgenic, Rats, Wistar, hormones, hormone substitutes, and hormone antagonists, Research Article
Abstract

Purpose Recently, arginine vasopressin (AVP) has been revealed to have diverse functional roles in nervous tissues beyond that of a vasoconstrictor. Several previous studies have indicated the existence of AVP in the retina, but the source of AVP has not been determined. The objective of the present study was to address the question of whether retinal cells have the ability to synthesize endogenous AVP to act in a paracrine or autocrine manner. Methods We used AVP-eGFP transgenic rats to find endogenous AVP-positive cells in the retina by immunohistochemistry with an AVP antibody and a GFP antibody. We also examined AVP mRNA and AVP receptor genes by reverse transcriptase (RT)–PCR of dissociated GFP-positive cells and whole retinas. Results Endogenous AVP-positive cells were found in the ganglion cell layer and inner nuclear layer of the retina of AVP-eGFP transgenic rats by immunohistochemistry. As indicated by the results of RT–PCR of dissociated GFP-positive cells, these cells have the ability to synthesize endogenous AVP, as well as transgenic AVP-eGFP. In addition, the V1a and V1b AVP receptors were found in the wild-type rat retina by whole retina RT–PCR, but the V2 receptor was not detectable. In dissociated AVP-eGFP-positive cells, no AVP receptor was detected by RT–PCR. Moreover, AVP secretion was not detected by stimulation with a high potassium (50 mM) solution. Conclusions In the rat retina, we found retinal cells that have the ability to synthesize endogenous AVP, and that the retina possesses V1a and V1b AVP receptors. Taken together, these results suggest that the retina has an intrinsic AVP-synthesizing and -receiving mechanism that can operate in a paracrine manner via V1a and V1b receptors.

Published
2011

13. The neuroprotective effect of hesperidin in NMDA-induced retinal injury acts by suppressing oxidative stress and excessive calpain activation

Author

Maekawa, Shigeto, primary, Sato, Kota, additional, Fujita, Kosuke, additional, Daigaku, Reiko, additional, Tawarayama, Hiroshi, additional, Murayama, Namie, additional, Moritoh, Satoru, additional, Yabana, Takeshi, additional, Shiga, Yukihiro, additional, Omodaka, Kazuko, additional, Maruyama, Kazuichi, additional, Nishiguchi, Koji M., additional, and Nakazawa, Toru, additional

Published
2017
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16. In Vivo Visualization of Subtle, Transient, and Local Activity of Astrocytes Using an Ultrasensitive Ca2+ Indicator

Author

Kanemaru, Kazunori, primary, Sekiya, Hiroshi, additional, Xu, Ming, additional, Satoh, Kaname, additional, Kitajima, Nami, additional, Yoshida, Keitaro, additional, Okubo, Yohei, additional, Sasaki, Takuya, additional, Moritoh, Satoru, additional, Hasuwa, Hidetoshi, additional, Mimura, Masaru, additional, Horikawa, Kazuki, additional, Matsui, Ko, additional, Nagai, Takeharu, additional, Iino, Masamitsu, additional, and Tanaka, Kenji F., additional

Published
2014
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21. RNAi-mediated Silencing of <it>OsGEN-L</it> (<it>OsGEN-like</it>), a New Member of the RAD2/XPG Nuclease Family, Causes Male Sterility by Defect of Microspore Development in Rice.

Author

Moritoh, Satoru, Miki, Daisuke, Akiyama, Masahiro, Kawahara, Mihoko, Izawa, Takeshi, Maki, Hisaji, and Shimamoto, Ko

Subjects
*PLANTS, *GREEN fluorescent protein, *PHYLOGENY, *NUCLEASES, *DNA, *MICROSPOREA, *RICE, *TRANSGENIC organisms
Abstract

We have cloned a new member of the RAD2/XPG nuclease family, OsGEN-L (OsGEN-like), from rice (Oryza sativa L.). OsGEN-L possesses two domains, the N- and I-regions, that are conserved in the RAD2/XPG nuclease family. Database searches and phylogenetic analyses revealed that OsGEN-L belongs to class 4 of the RAD2/XPG nuclease family, and OsGEN-L homologs were found in animals and higher plants. To elucidate the function of OsGEN-L, we generated rice OsGEN-L-RNAi transgenic plants in which OsGEN-L expression was silenced. Most of the OsGEN-L-RNAi plants displayed low fertility, and some of them were male-sterile. OsGEN-L-RNAi plants lacked mature pollen, resulting from a defect in early microspore development. A OsGEN-L–green fluorescent protein (GFP) fusion protein was localized in the nucleus, and the OsGEN-L promoter was specifically active in the anthers. Furthermore, a recombinant OsGEN-L protein possessed flap endonuclease activity and both single-stranded and double-stranded DNA-binding activities. Our results suggest that OsGEN-L plays an essential role in DNA metabolism required for early microspore development in rice. [ABSTRACT FROM AUTHOR]

Published
2005
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