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18. Experience Using Gentian Violet-Free Dyes for Tissue Visualization.

Author

Matano, Fumihiro, Murai, Yasuo, Nounaka, Yohei, Higuchi, Tadashi, Mihara, Riku, Isayama, Koshiro, and Morita, Akio

Subjects
GENTIAN violet, TEMPORAL arteries, DYES & dyeing, CEREBRAL arteries, BLOOD vessels, FREE flaps
Abstract

Background Gentian violet ink is used as a skin marker in various surgical procedures, including neurosurgery. The dye is also used to visualize the edges of blood vessels during bypass surgery. However, gentian violet ink carries the risks of carcinogenicity and venous injury, which causes microvascular thrombosis. In this study, we compare the gentian violet–free dye C.I. Basic Violet 4 (BV4) and gentian violet. The usefulness, in terms of color, and formation of microvascular thrombosis in anastomosis were compared. Methods We used the gentian violet–free dye in 20 cases involving 3 vascular anastomoses. The bone cutting lines on the bone surface, superior temporal artery, and middle cerebral artery were drawn using BV4 and gentian violet ink. Results The colors of BV4 and gentian violet ink were similar. No thrombus formation was observed at the vascular anastomosis when using BV4. Conclusion BV4 can be used similarly to gentian violet ink. No adverse effects such as thrombus formation in microvascular anastomosis were experienced when BV4 was used. [ABSTRACT FROM AUTHOR]

Published
2024
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29. Pathological Findings of Donor Vessels in Bypass Surgery.

Author

Nounaka, Yohei, Murai, Yasuo, Kubota, Asami, Tsukiyama, Atsushi, Matano, Fumihiro, Koketsu, Kenta, and Morita, Akio

Subjects
MOYAMOYA disease, RADIAL artery, ARTERIAL stenosis, TEMPORAL arteries, CEREBRAL revascularization, CAROTID intima-media thickness, ANKLE brachial index
Abstract

(1) Background Cerebral revascularization is necessary to treat intracranial arterial stenosis caused by moyamoya disease, atherosclerosis, or large complex aneurysms. Although various donor vascular harvesting methods have been reported safe, there are no reports on the histological evaluation of donor vessels for each disease, despite the variety of diseases wherein vascular anastomosis is required. (2) Methods Pathological findings of the superficial temporal artery (STA), radial artery (RA), occipital artery (OA), and saphenous vein (SV) harvested at the institution were analyzed. Patients classified according to aneurysm, atherosclerosis, and moyamoya disease were assessed for pathological abnormalities, medical history, age, sex, smoking, and postoperative anastomosis patency. (3) Results There were 38 cases of atherosclerosis, 15 cases of moyamoya disease, and 30 cases of aneurysm in 98 donor vessels (mean age 57.2) taken after 2006. Of the 84 STA, 11 RA, 2 OA, and 1 SV arteries that were harvested, 71.4% had atherosclerosis, 11.2% had dissection, and 10.2% had inflammation. There was no significant difference in the proportion of pathological findings according to the disease. A history of hypertension is associated with atherosclerosis in donor vessels. (4) Conclusions This is the first study to histologically evaluate the pathological findings of donor vessels according to disease. The proportion of dissection findings indicative of vascular damage due to surgical manipulation was not statistically different between the different conditions. [ABSTRACT FROM AUTHOR]

Published
2024
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40. Prosthesis Used in Microvascular Decompressions: A Multicenter Survey in Japan Focusing on Adverse Events

Author

Hasegawa, Mitsuhiro, Hatayama, Toru, Kondo, Akinori, Nagahiro, Shinji, Fujimaki, Takamitsu, Amagasaki, Kenichi, Arita, Kazunori, Date, Isao, Fujii, Yukihiko, Goto, Takeo, Hanaya, Ryosuke, Higuchi, Yoshinori, Hongo, Kazuhiro, Inoue, Toru, Kasuya, Hidetoshi, Kayama, Takamasa, Kawashima, Masatou, Kohmura, Eiji, Maehara, Taketoshi, Matsushima, Toshio, Mizobuchi, Yoshihumi, Morita, Akio, Nishizawa, Shigeru, Noro, Shusaku, Saito, Shinjiro, Shimano, Hirofumi, Shirane, Reizo, Takeshima, Hideo, Tanaka, Yuichiro, Tanabe, Hidenori, Toda, Hiroki, Yamakami, Iwao, Nishiyama, Yuya, Ohba, Shigeo, Hirose, Yuichi, and Suzuki, Takeya

Published
2019
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43. In-hospital mortality and poor outcome after surgical clipping and endovascular coiling for aneurysmal subarachnoid hemorrhage using nationwide databases: a systematic review and meta-analysis

Author

Ikawa, Fusao, Michihata, Nobuaki, Matsushige, Toshinori, Abiko, Masaru, Ishii, Daizo, Oshita, Jumpei, Okazaki, Takahito, Sakamoto, Shigeyuki, Kurogi, Ryota, Iihara, Koji, Nishimura, Kunihiro, Morita, Akio, Fushimi, Kiyohide, Yasunaga, Hideo, and Kurisu, Kaoru

Published
2020
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48. Nutritional characterization on growth and ionome profiles in Japanese wasabi cultivars (Eutrema japonicum) under hydroponics.

Author

Yamashita, Hiroto, Mihara, Harumi, Hisamatsu, Susumu, Morita, Akio, and Ikka, Takashi

Subjects
ROOT growth, HYDROPONICS, PLANT growth, NUTRITIONAL requirements, AMINO acids
Abstract

Japanese wasabi is traditionally cultivated in terraced fields with running stream water conditions which typically produce a high-quality of yield. Previous studies have explored some of the effects of water nutrient status under such conditions; however, the relationship between water nutrient status in these cultivation conditions and the growth and quality of wasabi is still completely unknown. We evaluated the effects of nutrient strength, inorganic nitrogen sources, and pH of the medium on the growth of wasabi plants. Then, we analyzed the photosynthetic capacity and the ionomes of two major Japanese wasabi cultivars ('Onimidori,' an early-growing green-stem type; and 'Mazuma No.1,' a late-growing red-stem type) grown under a range of pH conditions. These experiments were conducted using a hydroponic system. Wasabi plants showed the best shoot and root growth in 1/10- and 1/2-strength Hoagland's solution with a 50:50 ratio of NH4-N:NO3-N. On the basis of the accumulation patterns of NO3−, amino acids, and chlorophylls, the 1/10-strength nutrient solution provided optimal conditions for wasabi growth. For both wasabi cultivars, the best shoot and root growth was in medium at pH 6.0. The photosynthetic capacity of 'Onimidori' was greater than that of 'Mazuma No.1,' suggesting that this may contribute to the faster growth of 'Onimidori.' Ionome analyses revealed tissue-specific mineral accumulation patterns and their differences among different pH conditions and between the two cultivars. Ionome-based multiple-regression analysis revealed a relationship between element concentration profiles in the wasabi plants, especially those in root, and the shoot growth. Wasabi plants are ammonium-sensitive and nitrate-preferring plants, in addition to relatively low nutrient requirements for optimal growth. The results of this study describe the basic nutritional characteristics of wasabi plants. These findings represent an important step toward optimizing fertilization to control wasabi quality and growth in the traditional flooded cultivation system. [ABSTRACT FROM AUTHOR]

Published
2024
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