20 results on '"Morente, M. M."'
Search Results
2. TuBaFrost: European Virtual Tumor Tissue Banking
- Author
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Riegman, P. H. J., primary, Oomen, M. H. A., additional, Dinjens, W. N. M., additional, Oosterhuis, J. W., additional, Lam, K. H., additional, Spatz, A., additional, Ratcliffe, C., additional, Knox, K., additional, Mager, R., additional, Kerr, D., additional, Pezzella, F., additional, van Damme, B., additional, van de Vijver, M., additional, van Boven, H., additional, Morente, M. M., additional, Alonso, S., additional, Kerjaschki, D., additional, Pammer, J., additional, López-Guerrero, J. A., additional, Llombart-Bosch, A., additional, Carbone, A., additional, Gloghini, A., additional, Teodorovic, I., additional, Isabelle, M., additional, Passioukov, A., additional, Lejeune, S., additional, Therasse, P., additional, and van Veen, E.-B., additional more...
- Published
- 2006
- Full Text
- View/download PDF
Catalog
3. Tumour banks and oncological research
- Author
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Morente, M. M.
- Published
- 2003
- Full Text
- View/download PDF
4. TuBaFrost 2: Standardising tissue collection and quality control procedures for a European virtual frozen tissue bank network
- Author
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UCL - SC/CHIM - Département de chimie, UCL - FSA/MAPR - Département des sciences des matériaux et des procédés, Morente, M. M., Lam, Kevin, Lejeune, Suzy, UCL - SC/CHIM - Département de chimie, UCL - FSA/MAPR - Département des sciences des matériaux et des procédés, Morente, M. M., Lam, Kevin, and Lejeune, Suzy more...
- Abstract
Tumour Bank Networking presents a great challenge for oncological research as in order to carry out large-scale, multi-centre studies with minimal intrinsic bias, each tumour bank in the network must have some fundamental similarities and be using the same standardised and validated procedures. The European Human Frozen Tumour Tissue Bank (TuBaFrost) has responded to this need by the promotion of an integrated platform of tumour banks in Europe. The operational framework for TuBaFrost has drawn upon the best practice of standard workflows and operating procedures employed by members of the TuBaFrost project and key initiatives worldwide. (c) 2006 Elsevier Ltd. All rights reserved. more...
- Published
- 2006
5. TuBaFrost: European Virtual Tumor Tissue Banking.
- Author
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Llombart-Bosch, Antonio, Felipo, Vicente, López-Guerrero, José Antonio, Riegman, P. H. J., Oomen, M. H. A., Dinjens, W. N. M., Oosterhuis, J. W., Lam, K. H., Spatz, A., Ratcliffe, C., Knox, K., Mager, R., Kerr, D., Pezzella, F., Damme, B., Vijver, M., Boven, H., Morente, M. M., Alonso, S., and Kerjaschki, D. more...
- Published
- 2006
- Full Text
- View/download PDF
6. Virtual microscopy in virtual tumor banking
- Author
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Isabelle, M., Teodorovic, I., Oosterhuis, J. W., Riegman, P. H., Passioukov, A., Lejeune, S., Therasse, P., Dinjens, W. N., Lam, K. H., Oomen, M. H., Spatz, A., Ratcliffe, C., Knox, K., Mager, R., Kerr, D., Pezzella, F., Damme, B., Vijver, M., Boven, H., Morente, M. M., Alonso, S., Kerjaschki, D., Pammer, J., López-Guerrero, J. A., Llombart-Bosch, A., Carbone, A., Annunziata Gloghini, Veen, E. B., and Pathology more...
- Subjects
Europe ,Microscopy ,Pathology, Clinical ,Databases, Factual ,Neoplasms ,ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION ,Frozen Sections ,Humans ,Tissue Banks - Abstract
Many systems have already been designed and successfully used for sharing histology images over large distances, without transfer of the original glass slides. Rapid evolution was seen when digital images could be transferred over the Internet. Nowadays, sophisticated virtual microscope systems can be acquired, with the capability to quickly scan large batches of glass slides at high magnification and compress and store the large images on disc, which subsequently can be consulted through the Internet. The images are stored on an image server, which can give simple, easy to transfer pictures to the user specifying a certain magnification on any position in the scan. This offers new opportunities in histology review, overcoming the necessity of the dynamic telepathology systems to have compatible software systems and microscopes and in addition, an adequate connection of sufficient bandwidth. Consulting the images now only requires an Internet connection and a computer with a high quality monitor. A system of complete pathology review supporting biorepositories is described, based on the implementation of this technique in the European Human Frozen Tumor Tissue Bank (TuBaFrost). more...
7. Tubafrost: Ruropean virtual tumor tissue banking
- Author
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Veen, E. -B, Therasse, P., Lejeune, S., Passioukov, A., Isabelle, M., Teodorovic, I., Annunziata Gloghini, Carbone, A., Llombart-Boschi, A., Lopez-Guerrero, J. A., Pammer, J., Kerjaschki, D., Alonso, S., Morente, M. M., Boven, H., Vijver, M., Damme, B., Pezzella, F., Kerr, D., Mager, R., Knox, K., Ratcliffe, C., Spatz, A., Lam, K. H., Oosterhuis, J. W., Dinjens, W. N. M., Oomen, M. H. A., and Riegman, P. H. more...
8. OECI TuBaFrost tumor biobanking
- Author
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Riegman, P. H. J., Llombart Bosch, A., Dinjens, W. N. M., Oomen, M. H. A., Spatz, A., Ratcliffe, C., Knox, K., Mager, R., Kerr, D., Pezzella, F., Damme, B., Vijver, M., Boven, H., Morente, M. M., Alonso, S., Kerjaschki, D., Pammer, J., Lopez-Guerrero, J. A., Antonino Carbone, Gloghini, A., Teodorovic, I., Isabelle, M., Jaminé, D., Passioukov, A., Lejeune, S., Therasse, P., Veen, E. B., Lam, K. H., and Oosterhuis, J. W. more...
9. Adverse clinical outcome in Hodgkin's disease is associated with loss of retinoblastoma protein expression, high Ki67 proliferation index, and absence of Epstein-Barr Virus-latent membrane protein 1 expression
- Author
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Víctor Abraira, Acevedo, A., Aguilera, B., Bellas, C., Fraga, M., Garcia-Del-Moral, R., Gomez-Marcos, F., Menarguez, J., Montalban, C., Morente, M. M., Oliva, H., Piris, M. A., and Sanchez-Beato, M.
- Subjects
Adult ,Male ,Adolescent ,Lewis X Antigen ,Middle Aged ,Prognosis ,Hodgkin Disease ,Retinoblastoma Protein ,Survival Analysis ,Viral Matrix Proteins ,Ki-67 Antigen ,Proto-Oncogene Proteins c-bcl-2 ,Child, Preschool ,Multivariate Analysis ,Humans ,Regression Analysis ,Female ,Tumor Suppressor Protein p53 ,Child ,Cell Division ,Aged ,Retrospective Studies - Abstract
Previous studies have shown that in non-Hodgkin's lymphomas and others neoplasms, tumoral progression, treatment response, and outcome are related to the expression of different oncogenic and tumor suppressor proteins. This study aimed to determine the prognostic significance of the expression of p53, bcl2, retinoblastoma protein (Rb), Ki67, CD15, and latent membrane protein 1-Epstein-Barr Virus (LMP1-EBV) proteins in Hodgkin's disease. A retrospective study was performed on 140 patients collected at the 11 participating centers belonging to the Spanish Collaborative Group for the Study of Hodgkin's Disease. A highly sensitive immunohistochemical method with previous microwave-induced antigen retrieval technique was used for the demonstration of the above-mentioned proteins. A Cox's multivariate analysis was performed to evaluate the impact of the variables in the overall survival, together with a logistic regression model for the achievement of complete remission. Univariate statistical analysis confirmed the prognostic significance of the alredy known clinical parameters: stage, age over 60 years, and B symptoms. High proliferation index (Ki67) and loss of Rb expression were also found to be adverse prognostic factors influencing respectively lower overall survival and failure to achieve complete remission. Multivariate analysis confirmed the independent significance of these two parameters and additionally identifies LMP1-EBV expression as a favorable prognostic marker, in relation with overall survival. Histopathological type, p53, bcl2, and CD15 expression lack significant influence on the outcome of this series. The progression of the disease or the response to treatment in HD patients is the consequence of the interrelationship of different factors, among which LMP1 expression, loss of Rb, and high growth fraction seems to play a more relevant role. more...
10. Standard operating procedure for the collection of fresh frozen tissue samples.
- Author
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Mager SR, Oomen MH, Morente MM, Ratcliffe C, Knox K, Kerr DJ, Pezzella F, and Riegman PH
- Subjects
- Humans, Quality Assurance, Health Care, Safety Management, Tissue Banks, Tissue and Organ Harvesting standards, Cryopreservation standards, Human Experimentation standards, Neoplasms pathology, Surgical Procedures, Operative standards, Tissue and Organ Harvesting methods
- Abstract
Studies using fresh-frozen tissue samples originating from different centres, as is often the case in EORTC related translational research, can show conflicting research results due to heterogeneity in the quality of samples and associated data from each centre. The development of infrastructure for the European Human Frozen Tumour Tissue Bank (TuBaFrost) anticipated this problem and Standard Operating Procedures (SOPs) have been developed to ensure samples collected are of consistent high quality and variation in research results is minimised. The SOPs drew on the best practice standard workflows and operating procedures employed by members of the TuBaFrost Consortium and key tissue bank initiatives worldwide. It was essential to provide workable solutions that reflect the variety in infrastructure and resources at the potential collecting centres and also the fact that it is not necessary to standardise every step of the collection and storage process in order to collect high quality tissue. Hence, the TuBaFrost SOPs detail the compulsory measures that must be implemented in order to become a TuBaFrost collecting centre and also make advisory recommendations regarding the less critical factors. Accordingly, the TuBaFrost SOPs are very flexible and to illustrate this the complete SOP for collecting, freezing and storing tissue at the Erasmus MC Tissue Bank is included. These TuBaFrost SOPs could equally be applicable to centres collecting samples for EORTC related translational research studies in order to standardise sample quality and produce reliable and reproducible research results. more...
- Published
- 2007
- Full Text
- View/download PDF
11. Tumour banking: the Spanish design.
- Author
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Morente MM, de Alava E, and Fernandez PL
- Subjects
- Animals, Cell Line, Tumor, Cooperative Behavior, Disease Models, Animal, Humans, Spain, Tissue Banks trends, Medical Oncology methods, Neoplasms pathology, Tissue Banks organization & administration
- Abstract
In the last decade the technical advances in high throughput techniques to analyze DNA, RNA and proteins have had a potential major impact on prevention, diagnosis, prognosis and treatment of many human diseases. Key pieces in this process, mainly thinking about the future, are tumour banks and tumour bank networks. To face these challenges, diverse suitable models and designs can be developed. The current article presents the development of a nationwide design of tumour banks in Spain based on a network of networks, specially focusing on its harmonization efforts mainly regarding technical procedures, ethical requirements, unified quality control policy and unique sample identification. We also describe our most important goals for the next years. This model does not correspond to a central tumour bank, but to a cooperative and coordinated network of national and regional networks. Independently from the network in which it is included, sample collections reside in their original institution, where it can be used for further clinical diagnosis, teaching and research activities of each independent hospital. The herein described 'network of networks' functional model could be useful for other countries and/or international tumour bank activities., ((c) 2007 S. Karger AG, Basel.) more...
- Published
- 2007
- Full Text
- View/download PDF
12. TuBaFrost 6: virtual microscopy in virtual tumour banking.
- Author
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Teodorovic I, Isabelle M, Carbone A, Passioukov A, Lejeune S, Jaminé D, Therasse P, Gloghini A, Dinjens WN, Lam KH, Oomen MH, Spatz A, Ratcliffe C, Knox K, Mager R, Kerr D, Pezzella F, van Damme B, van de Vijver M, van Boven H, Morente MM, Alonso S, Kerjaschki D, Pammer J, Lopez-Guerrero JA, Llombart Bosch A, van Veen EB, Oosterhuis JW, and Riegman PH more...
- Subjects
- Computer Simulation, Europe, Forecasting, Humans, Information Storage and Retrieval, Registries, Databases as Topic organization & administration, Frozen Sections, Microscopy methods, Neoplasms pathology, Pathology, Clinical organization & administration, Tissue Banks organization & administration
- Abstract
Many systems have already been designed and successfully used for sharing histology images over large distances, without transfer of the original glass slides. Rapid evolution was seen when digital images could be transferred over the Internet. Nowadays, sophisticated Virtual Microscope systems can be acquired, with the capability to quickly scan large batches of glass slides at high magnification and compress and store the large images on disc, which subsequently can be consulted through the Internet. The images are stored on an image server, which can give simple, easy to transfer pictures to the user specifying a certain magnification on any position in the scan. This offers new opportunities in histology review, overcoming the necessity of the dynamic telepathology systems to have compatible software systems and microscopes and in addition, an adequate connection of sufficient bandwidth. Consulting the images now only requires an Internet connection and a computer with a high quality monitor. A system of complete pathology review supporting bio-repositories is described, based on the implementation of this technique in the European Human Frozen Tumor Tissue Bank (TuBaFrost). more...
- Published
- 2006
- Full Text
- View/download PDF
13. TuBaFrost 5: multifunctional central database application for a European tumor bank.
- Author
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Isabelle M, Teodorovic I, Morente MM, Jaminé D, Passioukov A, Lejeune S, Therasse P, Dinjens WN, Oosterhuis JW, Lam KH, Oomen MH, Spatz A, Ratcliffe C, Knox K, Mager R, Kerr D, Pezzella F, van de Vijver M, van Boven H, Alonso S, Kerjaschki D, Pammer J, Lopez-Guerrero JA, Llombart Bosch A, Carbone A, Gloghini A, van Veen EB, van Damme B, and Riegman PH more...
- Subjects
- Computer Simulation, Europe, Forecasting, Humans, Information Storage and Retrieval, Registries, Databases as Topic organization & administration, Frozen Sections, Neoplasms pathology, Pathology, Clinical organization & administration, Tissue Banks organization & administration
- Abstract
Developing a tissue bank database has become more than just logically arranging data in tables combined with a search engine. Current demand for high quality samples and data, and the ever-changing legal and ethical regulations mean that the application must reflect TuBaFrost rules and protocols for the collection, exchange and use of tissue. To ensure continuation and extension of the TuBaFrost European tissue bank, the custodianship of the samples, and hence the decision over whether to issue samples to requestors, remains with the local collecting centre. The database application described in this article has been developed to facilitate this open structure virtual tissue bank model serving a large group. It encompasses many key tasks, without the requirement for personnel, hence minimising operational costs. The Internet-accessible database application enables search, selection and request submission for requestors, whereas collectors can upload and edit their collection. Communication between requestor and involved collectors is started with automatically generated e-mails. more...
- Published
- 2006
- Full Text
- View/download PDF
14. TuBaFrost 1: Uniting local frozen tumour banks into a European network: an overview.
- Author
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Riegman PH, Dinjens WN, Oomen MH, Spatz A, Ratcliffe C, Knox K, Mager R, Kerr D, Pezzella F, van Damme B, van de Vijver M, van Boven H, Morente MM, Alonso S, Kerjaschki D, Pammer J, Lopez-Guerrero JA, Llombart Bosch A, Carbone A, Gloghini A, Teodorovic I, Isabelle M, Jaminé D, Passioukov A, Lejeune S, Therasse P, van Veen EB, Lam KH, and Oosterhuis JW more...
- Subjects
- Biological Specimen Banks ethics, Biological Specimen Banks legislation & jurisprudence, Biological Specimen Banks standards, Computer Simulation, Databases, Factual standards, Ethics, Research, Europe, Forecasting, Humans, Internet, Quality Control, Biological Specimen Banks organization & administration, Cryopreservation, International Cooperation, Neoplasms pathology
- Abstract
TuBaFrost is the consortium responsible for the creation of a virtual European human frozen tumour tissue bank: a collection of high quality frozen residual, accurately classified tumour tissue samples, which are stored in European cancer centres and universities. This virtual tissue bank, searchable on the internet, has rules for access and use, and a code of conduct to comply with the various legal and ethical regulations in European countries. The easy accessibility and the European scale of the bank will result in the availability of a large number of samples even of rarer tumour types. Standardisation of collection, storage and quality control throughout the network is achieved minimising inter-institutional variability. A website providing access to upload, search and request samples is a key tool of the tissue bank. The search engine makes use of virtual microscopy. An overview of the development of the European virtual frozen tissue bank infrastructure is described in this paper. The various key aspects are described in more detail in a series of articles to appear in this Journal. more...
- Published
- 2006
- Full Text
- View/download PDF
15. TuBaFrost 4: access rules and incentives for a European tumour bank.
- Author
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Lopez-Guerrero JA, Riegman PH, Oosterhuis JW, Lam KH, Oomen MH, Spatz A, Ratcliffe C, Knox K, Mager R, Kerr D, Pezzella F, van Damme B, van de Vijver M, van Boven H, Morente MM, Alonso S, Kerjaschki D, Pammer J, Carbone A, Gloghini A, Teodorovic I, Isabelle M, Passioukov A, Lejeune S, Therasse P, van Veen EB, Dinjens WN, and Llombart-Bosch A more...
- Subjects
- Europe, Humans, Interinstitutional Relations, Interprofessional Relations, Specimen Handling, Human Experimentation, Neoplasms, Tissue Banks statistics & numerical data
- Abstract
When designing infrastructure for a networked virtual tumour bank (samples remain at the collector institutes and sample data are collected in a searchable central database), it is apparent that this can only function properly after developing an adequate set of rules for use and access. These rules must include sufficient incentives for the tissue sample collectors to remain active within the network and maintain sufficient sample levels in the local bank. These requirements resulted in a key TuBaFrost rule, stating that the custodianship of the samples remains under the authority of the local collector. As a consequence, the samples and the decision to issue the samples to a requestor are not transferred to a large organisation but instead remain with the collector, thus allowing autonomous negotiation between collector and requestor, potential co-authorship in publications or compensation for collection and processing costs. Furthermore, it realises a streamlined cost effective network, ensuring tissue visibility and accessibility thereby improving the availability of large amounts of samples of highly specific or rare tumour types as well as providing contact opportunities for collaboration between scientists with cutting edge technology and tissue collectors. With this general purpose in mind, the rules and responsibilities for collectors, requestors and central office were generated. more...
- Published
- 2006
- Full Text
- View/download PDF
16. TuBaFrost 3: regulatory and ethical issues on the exchange of residual tissue for research across Europe.
- Author
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van Veen EB, Riegman PH, Dinjens WN, Lam KH, Oomen MH, Spatz A, Mager R, Ratcliffe C, Knox K, Kerr D, van Damme B, van de Vijver M, van Boven H, Morente MM, Alonso S, Kerjaschki D, Pammer J, Lopez-Guerrero JA, Llombart Bosch A, Carbone A, Gloghini A, Teodorovic I, Isabelle M, Passioukov A, Lejeune S, Therasse P, and Oosterhuis JW more...
- Subjects
- Ethics, Research, Europe, Human Experimentation ethics, Humans, Interinstitutional Relations, Interprofessional Relations ethics, Specimen Handling, Tissue Banks ethics, Human Experimentation legislation & jurisprudence, Neoplasms, Tissue Banks legislation & jurisprudence
- Abstract
The regulatory regimes for research with residual tissue and accompanying data differ widely between countries in the European Union (EU): from specific consent to opt-out or even no consent at all. This could greatly hamper research where the exchange of tissue and accompanying data has become the gold standard, like in TubaFrost. Instead of adhering to international guidelines, which have a democratic deficit, or an attempt for a new set of possible harmonising rules, TubaFrost chose to create a coordinating rule: if tissue may legitimately be used for a certain kind of research in the country where it was taken and under whose jurisdiction the patient falls, it may also be used for such research in the country where it is sent to in the context of a scientific program even if in that other country other regulations would apply for research with residual tissue taken from patients under their jurisdiction. This coordinating rule has a sound basis in EU law in general and will solve the problems related to diverging national regulatory regimes in the case of cross national research with residual tissue. more...
- Published
- 2006
- Full Text
- View/download PDF
17. TuBaFrost 2: Standardising tissue collection and quality control procedures for a European virtual frozen tissue bank network.
- Author
-
Morente MM, Mager R, Alonso S, Pezzella F, Spatz A, Knox K, Kerr D, Dinjens WN, Oosterhuis JW, Lam KH, Oomen MH, van Damme B, van de Vijver M, van Boven H, Kerjaschki D, Pammer J, Lopez-Guerrero JA, Llombart Bosch A, Carbone A, Gloghini A, Teodorovic I, Isabelle M, Passioukov A, Lejeune S, Therasse P, van Veen EB, Ratcliffe C, and Riegman PH more...
- Subjects
- Biopsy standards, Containment of Biohazards standards, Dissection standards, Europe, Humans, Quality Control, Time Factors, Biological Specimen Banks standards, Cryopreservation standards, International Cooperation, Neoplasms pathology, Specimen Handling standards
- Abstract
Tumour Bank Networking presents a great challenge for oncological research as in order to carry out large-scale, multi-centre studies with minimal intrinsic bias, each tumour bank in the network must have some fundamental similarities and be using the same standardised and validated procedures. The European Human Frozen Tumour Tissue Bank (TuBaFrost) has responded to this need by the promotion of an integrated platform of tumour banks in Europe. The operational framework for TuBaFrost has drawn upon the best practice of standard workflows and operating procedures employed by members of the TuBaFrost project and key initiatives worldwide. more...
- Published
- 2006
- Full Text
- View/download PDF
18. Virtual microscopy in virtual tumor banking.
- Author
-
Isabelle M, Teodorovic I, Oosterhuis JW, Riegman PH, Passioukov A, Lejeune S, Therasse P, Dinjens WN, Lam KH, Oomen MH, Spatz A, Ratcliffe C, Knox K, Mager R, Kerr D, Pezzella F, Van Damme B, Van de Vijver M, Van Boven H, Morente MM, Alonso S, Kerjaschki D, Pammer J, López-Guerrero JA, Llombart-Bosch A, Carbone A, Gloghini A, and Van Veen EB more...
- Subjects
- Europe, Frozen Sections, Humans, Microscopy, Databases, Factual, Neoplasms pathology, Pathology, Clinical organization & administration, Tissue Banks organization & administration
- Abstract
Many systems have already been designed and successfully used for sharing histology images over large distances, without transfer of the original glass slides. Rapid evolution was seen when digital images could be transferred over the Internet. Nowadays, sophisticated virtual microscope systems can be acquired, with the capability to quickly scan large batches of glass slides at high magnification and compress and store the large images on disc, which subsequently can be consulted through the Internet. The images are stored on an image server, which can give simple, easy to transfer pictures to the user specifying a certain magnification on any position in the scan. This offers new opportunities in histology review, overcoming the necessity of the dynamic telepathology systems to have compatible software systems and microscopes and in addition, an adequate connection of sufficient bandwidth. Consulting the images now only requires an Internet connection and a computer with a high quality monitor. A system of complete pathology review supporting biorepositories is described, based on the implementation of this technique in the European Human Frozen Tumor Tissue Bank (TuBaFrost). more...
- Published
- 2006
- Full Text
- View/download PDF
19. [The classification of the lymphomas: an advance of the cancer taxonomy in the 21st century].
- Author
-
Morente MM and Piris MA
- Subjects
- Humans, Medical Oncology trends, World Health Organization, Lymphoma classification
- Published
- 2003
- Full Text
- View/download PDF
20. Adverse clinical outcome in Hodgkin's disease is associated with loss of retinoblastoma protein expression, high Ki67 proliferation index, and absence of Epstein-Barr virus-latent membrane protein 1 expression.
- Author
-
Morente MM, Piris MA, Abraira V, Acevedo A, Aguilera B, Bellas C, Fraga M, Garcia-Del-Moral R, Gomez-Marcos F, Menarguez J, Oliva H, Sanchez-Beato M, and Montalban C
- Subjects
- Adolescent, Adult, Aged, Cell Division, Child, Child, Preschool, Female, Hodgkin Disease metabolism, Hodgkin Disease pathology, Humans, Lewis X Antigen metabolism, Male, Middle Aged, Multivariate Analysis, Prognosis, Proto-Oncogene Proteins c-bcl-2 metabolism, Regression Analysis, Retrospective Studies, Survival Analysis, Tumor Suppressor Protein p53 metabolism, Hodgkin Disease diagnosis, Ki-67 Antigen metabolism, Retinoblastoma Protein metabolism, Viral Matrix Proteins metabolism
- Abstract
Previous studies have shown that in non-Hodgkin's lymphomas and others neoplasms, tumoral progression, treatment response, and outcome are related to the expression of different oncogenic and tumor suppressor proteins. This study aimed to determine the prognostic significance of the expression of p53, bcl2, retinoblastoma protein (Rb), Ki67, CD15, and latent membrane protein 1-Epstein-Barr Virus (LMP1-EBV) proteins in Hodgkin's disease. A retrospective study was performed on 140 patients collected at the 11 participating centers belonging to the Spanish Collaborative Group for the Study of Hodgkin's Disease. A highly sensitive immunohistochemical method with previous microwave-induced antigen retrieval technique was used for the demonstration of the above-mentioned proteins. A Cox's multivariate analysis was performed to evaluate the impact of the variables in the overall survival, together with a logistic regression model for the achievement of complete remission. Univariate statistical analysis confirmed the prognostic significance of the alredy known clinical parameters: stage, age over 60 years, and B symptoms. High proliferation index (Ki67) and loss of Rb expression were also found to be adverse prognostic factors influencing respectively lower overall survival and failure to achieve complete remission. Multivariate analysis confirmed the independent significance of these two parameters and additionally identifies LMP1-EBV expression as a favorable prognostic marker, in relation with overall survival. Histopathological type, p53, bcl2, and CD15 expression lack significant influence on the outcome of this series. The progression of the disease or the response to treatment in HD patients is the consequence of the interrelationship of different factors, among which LMP1 expression, loss of Rb, and high growth fraction seems to play a more relevant role. more...
- Published
- 1997
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