228 results on '"Moreno-Moral A"'
Search Results
2. Single nuclei transcriptomics of the in situ human limbal stem cell niche
- Author
-
Kathryn C. Davidson, Minkyung Sung, Karl D. Brown, Julian Contet, Serena Belluschi, Regan Hamel, Aida Moreno-Moral, Rodrigo L. dos Santos, Julian Gough, Jose M. Polo, Mark Daniell, and Geraint J. Parfitt
- Subjects
Medicine ,Science - Abstract
Abstract The corneal epithelium acts as a barrier to pathogens entering the eye; corneal epithelial cells are continuously renewed by uni-potent, quiescent limbal stem cells (LSCs) located at the limbus, where the cornea transitions to conjunctiva. There has yet to be a consensus on LSC markers and their transcriptome profile is not fully understood, which may be due to using cadaveric tissue without an intact stem cell niche for transcriptomics. In this study, we addressed this problem by using single nuclei RNA sequencing (snRNAseq) on healthy human limbal tissue that was immediately snap-frozen after excision from patients undergoing cataract surgery. We identified the quiescent LSCs as a sub-population of corneal epithelial cells with a low level of total transcript counts. Moreover, TP63, KRT15, CXCL14, and ITGβ4 were found to be highly expressed in LSCs and transiently amplifying cells (TACs), which constitute the corneal epithelial progenitor populations at the limbus. The surface markers SLC6A6 and ITGβ4 could be used to enrich human corneal epithelial cell progenitors, which were also found to specifically express the putative limbal progenitor cell markers MMP10 and AC093496.1.
- Published
- 2024
- Full Text
- View/download PDF
3. Photoreceptor laminin drives differentiation of human pluripotent stem cells to photoreceptor progenitors that partially restore retina function
- Author
-
Tay, Hwee Goon, Andre, Helder, Chrysostomou, Vicki, Adusumalli, Swarnaseetha, Guo, Jing, Ren, Xiaoyuan, Tan, Wei Sheng, Tor, Jia En, Moreno-Moral, Aida, Plastino, Flavia, Bartuma, Hammurabi, Cai, Zuhua, Tun, Sai Bo Bo, Barathi, Veluchamy Amutha, Siew Wei, Gavin Tan, Grenci, Gianluca, Chong, Li Yen, Holmgren, Arne, Kvanta, Anders, Crowston, Jonathan Guy, Petretto, Enrico, and Tryggvason, Karl
- Published
- 2023
- Full Text
- View/download PDF
4. Immunolipidomics Reveals a Globoside Network During the Resolution of Pro-Inflammatory Response in Human Macrophages
- Author
-
Sneha Muralidharan, Federico Torta, Michelle K. Lin, Antoni Olona, Marta Bagnati, Aida Moreno-Moral, Jeong-Hun Ko, Shanshan Ji, Bo Burla, Markus R. Wenk, Hosana G. Rodrigues, Enrico Petretto, and Jacques Behmoaras
- Subjects
lipidomics ,human macrophages ,transcriptomics ,globosides ,network analysis ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Toll-like receptor 4 (TLR4)-mediated changes in macrophages reshape intracellular lipid pools to coordinate an effective innate immune response. Although this has been previously well-studied in different model systems, it remains incompletely understood in primary human macrophages. Here we report time-dependent lipidomic and transcriptomic responses to lipopolysaccharide (LPS) in primary human macrophages from healthy donors. We grouped the variation of ~200 individual lipid species measured by LC-MS/MS into eight temporal clusters. Among all other lipids, glycosphingolipids (glycoSP) and cholesteryl esters (CE) showed a sharp increase during the resolution phase (between 8h or 16h post LPS). GlycoSP, belonging to the globoside family (Gb3 and Gb4), showed the greatest inter-individual variability among all lipids quantified. Integrative network analysis between GlycoSP/CE levels and genome-wide transcripts, identified Gb4 d18:1/16:0 and CE 20:4 association with subnetworks enriched for T cell receptor signaling (PDCD1, CD86, PTPRC, CD247, IFNG) and DC-SIGN signaling (RAF1, CD209), respectively. Our findings reveal Gb3 and Gb4 globosides as sphingolipids associated with the resolution phase of inflammatory response in human macrophages.
- Published
- 2022
- Full Text
- View/download PDF
5. Integrative genetic and network approaches to identify key regulators of cardiac fibrosis
- Author
-
Moreno Moral, Aida, Petretto, Enrico, and Bottolo, Leonardo
- Subjects
610 - Abstract
Excessive fibrogenic response is a pathological hallmark of chronic complex diseases, including cardiovascular disease. To date, very few gene targets for cardiac fibrosis that led to effective treatments have been identified in humans. In this thesis I study and dissect the genetic component underlying cardiac fibrosis. This study integrates histomorphometric measurements of fibrosis in the rat left ventricle (LV) with gene expression (RNA-Seq from LV) and genetic data in a panel of recombinant inbred (RI) rat strains (n=30). In addition, I integrated RNA-seq LV and genetic data in humans (n=187, healthy and dilated cardiomyopathy (DCM) patients), as well as DCM genome-wide association studies (GWAS) data. I started by carrying out an unbiased co-expression network analysis in the rat heart. The reconstructed cardiac transcriptional modules were associated with quantitative levels of fibrosis. Co-expression networks were also independently built in the heart of DCM patients and by using the rat data, co-expression networks associated with fibrosis, conserved across rats and humans and not present in control human heart were prioritised. In the prioritised networks, I also analysed their cardiac cell type specificity, differential expression after TGFβ induction, potential driving transcription factors and conservation in other fibrotic diseases by analysing human data collected from other organs. Furthermore, I aimed to identify common genetic regulators of the networks (also called master genetic regulators) by using Bayesian multivariate regression approaches. Finally, I integrated GWAS data in DCM (n=2,287) to dissect the genetic basis of DCM. This systems genetics study evidences that there are transcriptional processes involved in the human cardiac fibrogenic response that are conserved across rats and humans, some of them also underlying DCM aetiology. In an attempt to suggest new gene targets for cardiac fibrosis, I also identified the WWP2 gene as a novel trans-acting genetic regulator of cardiac fibrosis.
- Published
- 2016
- Full Text
- View/download PDF
6. Effect of the content of microbial proteins and the poly-β-hydroxybutyric acid in biofloc on the performance and health of Nile tilapia (Oreochromis niloticus) fingerlings fed on a protein-restricted diet
- Author
-
Gullian Klanian, Mariel, Delgadillo Díaz, Mariana, Sánchez Solís, María José, Aranda, Javier, and Moreno Moral, Paloma
- Published
- 2020
- Full Text
- View/download PDF
7. Orobanche centaurina Bertol. the correct name for O. kochii F.W. Schultz (Orobanchaceae) [Orobanche centaurina Bertol., nombre correcto para O. kochii F.W. Schultz (Orobanchaceae)]
- Author
-
Jiří ZÁZVORKA, Óscar SÁNCHEZ PEDRAJA, Gonzalo MORENO MORAL, Luis CARLÓN RUIZ, Gianniantonio DOMINA, Manuel LAÍNZ GALLO, and Renata PIWOWARCZYK
- Subjects
nomenclature ,eurasian flora ,orobanchaceae ,parasitic plants ,nomenclatura ,flora euroasiática ,plantas parásitas ,Botany ,QK1-989 - Abstract
After the studying the original material of Orobanche centaurina Bertol. (Orobanchaceae) deposited in BOLO, a forgotten species described from Massa (Central Italy) parasitizing Centaurea paniculata L., and compared it with the type of O. kochii F.W. Schultz and with the types of others taxa actually considered synonyms of this, we concluded that the name of Bertoloni is the correct one for this species. Después de estudiar el material original de Orobanche centaurina Bertol. (Orobanchaceae) depo¬sitado en BOLO, una olvidada especie descrita de Massa (Italia central) parásita de Centaurea paniculata L., y com¬pararlo con el tipo de O. kochii F.W. Schultz y con los tipos de otros táxones actualmente considerados sinónimos de éste, estimamos que el nombre de Bertoloni es el correcto y prioritario para esta especie.
- Published
- 2019
8. WWP2 regulates pathological cardiac fibrosis by modulating SMAD2 signaling
- Author
-
Huimei Chen, Aida Moreno-Moral, Francesco Pesce, Nithya Devapragash, Massimiliano Mancini, Ee Ling Heng, Maxime Rotival, Prashant K. Srivastava, Nathan Harmston, Kirill Shkura, Owen J. L. Rackham, Wei-Ping Yu, Xi-Ming Sun, Nicole Gui Zhen Tee, Elisabeth Li Sa Tan, Paul J. R. Barton, Leanne E. Felkin, Enrique Lara-Pezzi, Gianni Angelini, Cristina Beltrami, Michal Pravenec, Sebastian Schafer, Leonardo Bottolo, Norbert Hubner, Costanza Emanueli, Stuart A. Cook, and Enrico Petretto
- Subjects
Science - Abstract
Pathological cardiac fibrosis is a hallmark of diseases leading to heart failure. Here, the authors used systems genetics to identify a pro-fibrotic gene network regulated by WWP2, a E3 ubiquitin ligase, which orchestrates the nucleocytoplasmic shuttling and transcriptional activity of SMAD2 in the diseased heart.
- Published
- 2019
- Full Text
- View/download PDF
9. Más, a propósito de algunas Phelipanche Pomel, Boulardia F. W. Schultz y Orobanche L. (Orobanchaceae) del oeste del Paleártico
- Author
-
Carlón Ruiz, Luis, Gómez Casares, Gonzalo, Laínz Gallo, Manuel, Moreno Moral, Gonzalo, Sánchez Pedraja, Óscar, Schneeweiss, Gerald M., Biblioteca Digital del Real Jardin Botanico de Madrid, Carlón Ruiz, Luis, Gómez Casares, Gonzalo, Laínz Gallo, Manuel, Moreno Moral, Gonzalo, Sánchez Pedraja, Óscar, and Schneeweiss, Gerald M.
- Subjects
Angiospermas ,Taxonomía - Published
- 2008
10. Phelipanche zangezuri (Orobanchaceae), a new species from the Caucasus, Armenia
- Author
-
Piwowarczyk, Renata, Sánchez-Pedraja, Óscar, Moreno-Moral, Gonzalo, Zakaryan, Narine, and Kartashyan, Nune
- Published
- 2018
11. Epoxygenase inactivation exacerbates diet and aging-associated metabolic dysfunction resulting from impaired adipogenesis
- Author
-
Olona, Antoni, Terra, Ximena, Ko, Jeong-Hun, Grau-Bové, Carme, Pinent, Montserrat, Ardevol, Anna, Diaz, Ana Garcia, Moreno-Moral, Aida, Edin, Matthew, Bishop-Bailey, David, Zeldin, Darryl C., Aitman, Timothy J., Petretto, Enrico, Blay, Mayte, and Behmoaras, Jacques
- Published
- 2018
- Full Text
- View/download PDF
12. High-dose corticosteroid pulse therapy increases the survival rate in COVID-19 patients at risk of hyper-inflammatory response.
- Author
-
Miguel Ángel López Zúñiga, Aida Moreno-Moral, Ana Ocaña-Granados, Francisco Andrés Padilla-Moreno, Alba María Castillo-Fernández, Dionisio Guillamón-Fernández, Carolina Ramírez-Sánchez, María Sanchez-Palop, Justo Martínez-Colmenero, María Amparo Pimentel-Villar, Sara Blázquez-Roselló, José Juan Moreno-Sánchez, María López-Vílchez, Inmaculada Prior-Sánchez, Rosario Jódar-Moreno, and Miguel Ángel López Ruz
- Subjects
Medicine ,Science - Abstract
ObjectiveTest whether high dose corticosteroid pulse therapy (HDCPT) with either methylprednisolone or dexamethasone is associated with increased survival in COVID-19 patients at risk of hyper-inflammatory response. Provide some initial diagnostic criteria using laboratory markers to stratify these patients.MethodsThis is a prospective observational study, 318 met the inclusion criteria. 64 patients (20.1%) were treated with HDCPT by using at least 1.5mg/kg/24h of methylprednisolone or dexamethasone equivalent. A multivariate Cox regression (controlling for co-morbidities and other therapies) was carried out to determine whether HDCPT (among other interventions) was associated with decreased mortality. We also carried out a 30-day time course analysis of laboratory markers between survivors and non-survivors, to identify potential markers for patient stratification.ResultsHDCPT showed a statistically significant decrease in mortality (HR = 0.087 [95% CI 0.021-0.36]; P < 0.001). 30-day time course analysis of laboratory marker tests showed marked differences in pro-inflammatory markers between survivors and non-survivors. As diagnostic criteria to define the patients at risk of developing a COVID-19 hyper-inflammatory response, we propose the following parameters (IL-6 > = 40 pg/ml, and/or two of the following: C-reactive protein > = 100 mg/L, D-dimer > = 1000 ng/ml, ferritin > = 500 ng/ml and lactate dehydrogenase > = 300 U/L).ConclusionsHDCPT can be an effective intervention to increase COVID-19 survival rates in patients at risk of developing a COVID-19 hyper-inflammatory response, laboratory marker tests can be used to stratify these patients who should be given HDCPT. This study is not a randomized clinical trial (RCT). Future RCTs should be carried out to confirm the efficacy of HDCPT to increase the survival rates of COVID-19.
- Published
- 2021
- Full Text
- View/download PDF
13. In Vivo Generation of Post-infarct Human Cardiac Muscle by Laminin-Promoted Cardiovascular Progenitors
- Author
-
Lynn Yap, Jiong-Wei Wang, Aida Moreno-Moral, Li Yen Chong, Yi Sun, Nathan Harmston, Xiaoyuan Wang, Suet Yen Chong, Konstantinos Vanezis, Miina K. Öhman, Heming Wei, Ralph Bunte, Sujoy Gosh, Stuart Cook, Outi Hovatta, Dominique P.V. de Kleijn, Enrico Petretto, and Karl Tryggvason
- Subjects
Biology (General) ,QH301-705.5 - Published
- 2020
- Full Text
- View/download PDF
14. Biologically relevant laminin as chemically defined and fully human platform for human epidermal keratinocyte culture
- Author
-
Monica Suryana Tjin, Alvin Wen Choong Chua, Aida Moreno-Moral, Li Yen Chong, Po Yin Tang, Nathan Peter Harmston, Zuhua Cai, Enrico Petretto, Bien Keem Tan, and Karl Tryggvason
- Subjects
Science - Abstract
In vitro expansion of human epidermal keratinocytes to resurface severe wound defects still relies on a human/mouse xenograft culture system. Here the authors develop a fully human, xeno-free culture system using skin-associated laminins, normally present in vivo, to replace mouse feeder cells.
- Published
- 2018
- Full Text
- View/download PDF
15. Epoxygenase inactivation exacerbates diet and aging-associated metabolic dysfunction resulting from impaired adipogenesis
- Author
-
Antoni Olona, Ximena Terra, Jeong-Hun Ko, Carme Grau-Bové, Montserrat Pinent, Anna Ardevol, Ana Garcia Diaz, Aida Moreno-Moral, Matthew Edin, David Bishop-Bailey, Darryl C. Zeldin, Timothy J. Aitman, Enrico Petretto, Mayte Blay, and Jacques Behmoaras
- Subjects
Internal medicine ,RC31-1245 - Abstract
Objective: When molecular drivers of healthy adipogenesis are perturbed, this can cause hepatic steatosis. The role of arachidonic acid (AA) and its downstream enzymatic cascades, such as cyclooxygenase, in adipogenesis is well established. The exact contribution of the P450 epoxygenase pathway, however, remains to be established. Enzymes belonging to this pathway are mainly encoded by the CYP2J locus which shows extensive allelic expansion in mice. Here we aimed to establish the role of endogenous epoxygenase during adipogenesis under homeostatic and metabolic stress conditions. Methods: We took advantage of the simpler genetic architecture of the Cyp2j locus in the rat and used a Cyp2j4 (orthologue of human CYP2J2) knockout rat in two models of metabolic dysfunction: physiological aging and cafeteria diet (CAF). The phenotyping of Cyp2j4−/− rats under CAF was integrated with proteomics (LC-MS/MS) and lipidomics (LC-MS) analyses in the liver and the adipose tissue. Results: We report that Cyp2j4 deletion causes adipocyte dysfunction under metabolic challenges. This is characterized by (i) down-regulation of white adipose tissue (WAT) PPARγ and C/EBPα, (ii) adipocyte hypertrophy, (iii) extracellular matrix remodeling, and (iv) alternative usage of AA pathway. Specifically, in Cyp2j4−/− rats treated with a cafeteria diet, the dysfunctional adipogenesis is accompanied by exacerbated weight gain, hepatic lipid accumulation, and dysregulated gluconeogenesis. Conclusion: These results suggest that AA epoxygenases are essential regulators of healthy adipogenesis. Our results uncover their synergistic role in fine-tuning AA pathway in obesity-mediated hepatic steatosis. Keywords: Adipogenesis, Cytochrome P450 2j4, Cafeteria diet, Aging, Steatosis, Arachidonic acid
- Published
- 2018
- Full Text
- View/download PDF
16. Phelipanche hajastanica (Orobanchaceae), a new species from Armenia
- Author
-
Piwowarczyk, Renata, Sánchez-Pedraja, Óscar, Moreno-Moral, Gonzalo, Nanagulyan, Siranush, Zakaryan, Narine, and Kartashyan, Nune
- Published
- 2017
17. IL-11 is a crucial determinant of cardiovascular fibrosis
- Author
-
Schafer, Sebastian, Viswanathan, Sivakumar, Widjaja, Anissa A., Lim, Wei-Wen, Moreno-Moral, Aida, DeLaughter, Daniel M., Ng, Benjamin, Patone, Giannino, Chow, Kingsley, Khin, Ester, Tan, Jessie, Chothani, Sonia P., Ye, Lei, Rackham, Owen J. L., Ko, Nicole S. J., Sahib, Norliza E., Pua, Chee Jian, Zhen, Nicole T. G., Xie, Chen, Wang, Mao, Maatz, Henrike, Lim, Shiqi, Saar, Kathrin, Blachut, Susanne, Petretto, Enrico, Schmidt, Sabine, Putoczki, Tracy, Guimares-Camboa, Nuno, Wakimoto, Hiroko, van Heesch, Sebastiaan, Sigmundsson, Kristmundur, Lim, See L., Soon, Jia L., Chao, Victor T. T., Chua, Yeow L., Tan, Teing E., Evans, Sylvia M., Loh, Yee J., Jamal, Muhammad H., Ong, Kim K., Chua, Kim C., Ong, Boon-Hean, Chakaramakkil, Mathew J., Seidman, Jonathan G., Seidman, Christine E., Hubner, Norbert, Sin, Kenny Y. K., and Cook, Stuart A.
- Subjects
Interleukins -- Health aspects ,Cardiovascular diseases -- Physiological aspects ,Fibrosis -- Physiological aspects ,Environmental issues ,Science and technology ,Zoology and wildlife conservation - Abstract
Author(s): Sebastian Schafer [1, 2]; Sivakumar Viswanathan [2]; Anissa A. Widjaja [2]; Wei-Wen Lim [1]; Aida Moreno-Moral [2]; Daniel M. DeLaughter [3]; Benjamin Ng [1]; Giannino Patone [4]; Kingsley Chow [...]
- Published
- 2017
- Full Text
- View/download PDF
18. Natural genetic variation of the cardiac transcriptome in non-diseased donors and patients with dilated cardiomyopathy
- Author
-
Matthias Heinig, Michiel E. Adriaens, Sebastian Schafer, Hanneke W. M. van Deutekom, Elisabeth M. Lodder, James S. Ware, Valentin Schneider, Leanne E. Felkin, Esther E. Creemers, Benjamin Meder, Hugo A. Katus, Frank Rühle, Monika Stoll, François Cambien, Eric Villard, Philippe Charron, Andras Varro, Nanette H. Bishopric, Alfred L. George, Cristobal dos Remedios, Aida Moreno-Moral, Francesco Pesce, Anja Bauerfeind, Franz Rüschendorf, Carola Rintisch, Enrico Petretto, Paul J. Barton, Stuart A. Cook, Yigal M. Pinto, Connie R. Bezzina, and Norbert Hubner
- Subjects
Genetics ,Gene expression ,eQTL ,Dilated cardiomyopathy ,Heart ,Biology (General) ,QH301-705.5 ,QH426-470 - Abstract
Abstract Background Genetic variation is an important determinant of RNA transcription and splicing, which in turn contributes to variation in human traits, including cardiovascular diseases. Results Here we report the first in-depth survey of heart transcriptome variation using RNA-sequencing in 97 patients with dilated cardiomyopathy and 108 non-diseased controls. We reveal extensive differences of gene expression and splicing between dilated cardiomyopathy patients and controls, affecting known as well as novel dilated cardiomyopathy genes. Moreover, we show a widespread effect of genetic variation on the regulation of transcription, isoform usage, and allele-specific expression. Systematic annotation of genome-wide association SNPs identifies 60 functional candidate genes for heart phenotypes, representing 20% of all published heart genome-wide association loci. Focusing on the dilated cardiomyopathy phenotype we found that eQTL variants are also enriched for dilated cardiomyopathy genome-wide association signals in two independent cohorts. Conclusions RNA transcription, splicing, and allele-specific expression are each important determinants of the dilated cardiomyopathy phenotype and are controlled by genetic factors. Our results represent a powerful resource for the field of cardiovascular genetics.
- Published
- 2017
- Full Text
- View/download PDF
19. Systems biology of the IMIDIA biobank from organ donors and pancreatectomised patients defines a novel transcriptomic signature of islets from individuals with type 2 diabetes
- Author
-
Solimena, Michele, Schulte, Anke M., Marselli, Lorella, Ehehalt, Florian, Richter, Daniela, Kleeberg, Manuela, Mziaut, Hassan, Knoch, Klaus-Peter, Parnis, Julia, Bugliani, Marco, Siddiq, Afshan, Jörns, Anne, Burdet, Frédéric, Liechti, Robin, Suleiman, Mara, Margerie, Daniel, Syed, Farooq, Distler, Marius, Grützmann, Robert, Petretto, Enrico, Moreno-Moral, Aida, Wegbrod, Carolin, Sönmez, Anke, Pfriem, Katja, Friedrich, Anne, Meinel, Jörn, Wollheim, Claes B., Baretton, Gustavo B., Scharfmann, Raphael, Nogoceke, Everson, Bonifacio, Ezio, Sturm, Dorothée, Meyer-Puttlitz, Birgit, Boggi, Ugo, Saeger, Hans-Detlev, Filipponi, Franco, Lesche, Mathias, Meda, Paolo, Dahl, Andreas, Wigger, Leonore, Xenarios, Ioannis, Falchi, Mario, Thorens, Bernard, Weitz, Jürgen, Bokvist, Krister, Lenzen, Sigurd, Rutter, Guy A., Froguel, Philippe, von Bülow, Manon, Ibberson, Mark, and Marchetti, Piero
- Published
- 2017
- Full Text
- View/download PDF
20. In Vivo Generation of Post-infarct Human Cardiac Muscle by Laminin-Promoted Cardiovascular Progenitors
- Author
-
Lynn Yap, Jiong-Wei Wang, Aida Moreno-Moral, Li Yen Chong, Yi Sun, Nathan Harmston, Xiaoyuan Wang, Suet Yen Chong, Miina K. Öhman, Heming Wei, Ralph Bunte, Sujoy Gosh, Stuart Cook, Outi Hovatta, Dominique P.V. de Kleijn, Enrico Petretto, and Karl Tryggvason
- Subjects
Biology (General) ,QH301-705.5 - Abstract
Summary: Regeneration of injured human heart muscle is limited and an unmet clinical need. There are no methods for the reproducible generation of clinical-quality stem cell-derived cardiovascular progenitors (CVPs). We identified laminin-221 (LN-221) as the most likely expressed cardiac laminin. We produced it as human recombinant protein and showed that LN-221 promotes differentiation of pluripotent human embryonic stem cells (hESCs) toward cardiomyocyte lineage and downregulates pluripotency and teratoma-associated genes. We developed a chemically defined, xeno-free laminin-based differentiation protocol to generate CVPs. We show high reproducibility of the differentiation protocol using time-course bulk RNA sequencing developed from different hESC lines. Single-cell RNA sequencing of CVPs derived from hESC lines supported reproducibility and identified three main progenitor subpopulations. These CVPs were transplanted into myocardial infarction mice, where heart function was measured by echocardiogram and human heart muscle bundle formation was identified histologically. This method may provide clinical-quality cells for use in regenerative cardiology. : Yap et al. perform in vitro differentiation of pluripotent embryonic stem cells to cardiovascular progenitors using laminin-221 as a culture matrix in a fully human and chemically defined protocol. Use of these cells for in vivo cardiac regeneration and to generate human heart muscle bundles results in improved heart function. Keywords: laminin, hESC-based cardiac muscle regeneration, regenerative cardiology, cardiovascular progenitors
- Published
- 2019
- Full Text
- View/download PDF
21. Systems Genetics Approaches in Rat Identify Novel Genes and Gene Networks Associated With Cardiac Conduction
- Author
-
Michiel E. Adriaens, Elisabeth M. Lodder, Aida Moreno‐Moral, Jan Šilhavý, Matthias Heinig, Charlotte Glinge, Charly Belterman, Rianne Wolswinkel, Enrico Petretto, Michal Pravenec, Carol Ann Remme, and Connie R. Bezzina
- Subjects
bioinformatics ,electrophysiology ,rats ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Background Electrocardiographic (ECG) parameters are regarded as intermediate phenotypes of cardiac arrhythmias. Insight into the genetic underpinnings of these parameters is expected to contribute to the understanding of cardiac arrhythmia mechanisms. Here we used HXB/BXH recombinant inbred rat strains to uncover genetic loci and candidate genes modulating ECG parameters. Methods and Results RR interval, PR interval, QRS duration, and QTc interval were measured from ECGs obtained in 6 male rats from each of the 29 available HXB/BXH recombinant inbred strains. Genes at loci displaying significant quantitative trait loci (QTL) effects were prioritized by assessing the presence of protein‐altering variants, and by assessment of cis expression QTL (eQTL) effects and correlation of transcript abundance to the respective trait in the heart. Cardiac RNA‐seq data were additionally used to generate gene co‐expression networks. QTL analysis of ECG parameters identified 2 QTL for PR interval, respectively, on chromosomes 10 and 17. At the chromosome 10 QTL, cis‐eQTL effects were identified for Acbd4, Cd300lg, Fam171a2, and Arhgap27; the transcript abundance in the heart of these 4 genes was correlated with PR interval. At the chromosome 17 QTL, a cis‐eQTL was uncovered for Nhlrc1 candidate gene; the transcript abundance of this gene was also correlated with PR interval. Co‐expression analysis furthermore identified 50 gene networks, 6 of which were correlated with PR interval or QRS duration, both parameters of cardiac conduction. Conclusions These newly identified genetic loci and gene networks associated with the ECG parameters of cardiac conduction provide a starting point for future studies with the potential of identifying novel mechanisms underlying cardiac electrical function.
- Published
- 2018
- Full Text
- View/download PDF
22. MT-HESS: an efficient Bayesian approach for simultaneous association detection in OMICS datasets, with application to eQTL mapping in multiple tissues.
- Author
-
Alex Lewin, Habib Saadi, James E. Peters, Aida Moreno-Moral, James C. Lee, Kenneth G. C. Smith, Enrico Petretto, Leonardo Bottolo, and Sylvia Richardson
- Published
- 2016
- Full Text
- View/download PDF
23. Author Correction: WWP2 regulates pathological cardiac fibrosis by modulating SMAD2 signaling
- Author
-
Chen, Huimei, Moreno-Moral, Aida, Pesce, Francesco, Devapragash, Nithya, Mancini, Massimiliano, Heng, Ee Ling, Rotival, Maxime, Srivastava, Prashant K., Harmston, Nathan, Shkura, Kirill, Rackham, Owen J. L., Yu, Wei-Ping, Sun, Xi-Ming, Tee, Nicole Gui Zhen, Tan, Elisabeth Li Sa, Barton, Paul J. R., Felkin, Leanne E., Lara-Pezzi, Enrique, Angelini, Gianni, Beltrami, Cristina, Pravenec, Michal, Schafer, Sebastian, Bottolo, Leonardo, Hubner, Norbert, Emanueli, Costanza, Cook, Stuart A., and Petretto, Enrico
- Published
- 2019
- Full Text
- View/download PDF
24. WWP2 regulates pathological cardiac fibrosis by modulating SMAD2 signaling
- Author
-
Chen, Huimei, Moreno-Moral, Aida, Pesce, Francesco, Devapragash, Nithya, Mancini, Massimiliano, Heng, Ee Ling, Rotival, Maxime, Srivastava, Prashant K., Harmston, Nathan, Shkura, Kirill, Rackham, Owen J. L., Yu, Wei-Ping, Sun, Xi-Ming, Tee, Nicole Gui Zhen, Tan, Elisabeth Li Sa, Barton, Paul J. R., Felkin, Leanne E., Lara-Pezzi, Enrique, Angelini, Gianni, Beltrami, Cristina, Pravenec, Michal, Schafer, Sebastian, Bottolo, Leonardo, Hubner, Norbert, Emanueli, Costanza, Cook, Stuart A., and Petretto, Enrico
- Published
- 2019
- Full Text
- View/download PDF
25. From integrative genomics to systems genetics in the rat to link genotypes to phenotypes
- Author
-
Aida Moreno-Moral and Enrico Petretto
- Subjects
Integrative genomics ,Systems genetics ,eQTL ,Gene network ,Rat ,Medicine ,Pathology ,RB1-214 - Abstract
Complementary to traditional gene mapping approaches used to identify the hereditary components of complex diseases, integrative genomics and systems genetics have emerged as powerful strategies to decipher the key genetic drivers of molecular pathways that underlie disease. Broadly speaking, integrative genomics aims to link cellular-level traits (such as mRNA expression) to the genome to identify their genetic determinants. With the characterization of several cellular-level traits within the same system, the integrative genomics approach evolved into a more comprehensive study design, called systems genetics, which aims to unravel the complex biological networks and pathways involved in disease, and in turn map their genetic control points. The first fully integrated systems genetics study was carried out in rats, and the results, which revealed conserved trans-acting genetic regulation of a pro-inflammatory network relevant to type 1 diabetes, were translated to humans. Many studies using different organisms subsequently stemmed from this example. The aim of this Review is to describe the most recent advances in the fields of integrative genomics and systems genetics applied in the rat, with a focus on studies of complex diseases ranging from inflammatory to cardiometabolic disorders. We aim to provide the genetics community with a comprehensive insight into how the systems genetics approach came to life, starting from the first integrative genomics strategies [such as expression quantitative trait loci (eQTLs) mapping] and concluding with the most sophisticated gene network-based analyses in multiple systems and disease states. Although not limited to studies that have been directly translated to humans, we will focus particularly on the successful investigations in the rat that have led to primary discoveries of genes and pathways relevant to human disease.
- Published
- 2016
- Full Text
- View/download PDF
26. Systems Genetics as a Tool to Identify Master Genetic Regulators in Complex Disease
- Author
-
Moreno-Moral, Aida, primary, Pesce, Francesco, additional, Behmoaras, Jacques, additional, and Petretto, Enrico, additional
- Published
- 2016
- Full Text
- View/download PDF
27. Biologically relevant laminin as chemically defined and fully human platform for human epidermal keratinocyte culture
- Author
-
Tjin, Monica Suryana, Chua, Alvin Wen Choong, Moreno-Moral, Aida, Chong, Li Yen, Tang, Po Yin, Harmston, Nathan Peter, Cai, Zuhua, Petretto, Enrico, Tan, Bien Keem, and Tryggvason, Karl
- Published
- 2018
- Full Text
- View/download PDF
28. Author Correction: WWP2 regulates pathological cardiac fibrosis by modulating SMAD2 signaling
- Author
-
Huimei Chen, Aida Moreno-Moral, Francesco Pesce, Nithya Devapragash, Massimiliano Mancini, Ee Ling Heng, Maxime Rotival, Prashant K. Srivastava, Nathan Harmston, Kirill Shkura, Owen J. L. Rackham, Wei-Ping Yu, Xi-Ming Sun, Nicole Gui Zhen Tee, Elisabeth Li Sa Tan, Paul J. R. Barton, Leanne E. Felkin, Enrique Lara-Pezzi, Gianni Angelini, Cristina Beltrami, Michal Pravenec, Sebastian Schafer, Leonardo Bottolo, Norbert Hubner, Costanza Emanueli, Stuart A. Cook, and Enrico Petretto
- Subjects
Science - Abstract
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
- Published
- 2019
- Full Text
- View/download PDF
29. Immunolipidomics Reveals a Globoside Network During the Resolution of Pro-Inflammatory Response in Human Macrophages
- Author
-
Muralidharan, Sneha, primary, Torta, Federico, additional, Lin, Michelle K., additional, Olona, Antoni, additional, Bagnati, Marta, additional, Moreno-Moral, Aida, additional, Ko, Jeong-Hun, additional, Ji, Shanshan, additional, Burla, Bo, additional, Wenk, Markus R., additional, Rodrigues, Hosana G., additional, Petretto, Enrico, additional, and Behmoaras, Jacques, additional
- Published
- 2022
- Full Text
- View/download PDF
30. Phelipanche cernua Pomel (Orobanchaceae), a prioritary name for the western mediterranean species recently redescribed as Ph. inexpectata [Phelipanche cernua Pomel (Orobanchaceae), un nombre prioritario para la especie del Mediterráneo Occidental recientemente descrita como Ph. inexspectata]
- Author
-
Luis Carlón Ruiz, Manuel Laínz Gallo, Gonzalo Moreno Moral, and Oscar Sánchez Pedraja
- Subjects
Phelipanche inexspectata ,Phelipanche cernua ,Phelipanche schultzii ,holoparasitic Orobanchaceae ,Lactuca ,Algeria ,France ,Morocco ,Spain ,holoparásitas ,Argelia ,España ,Francia ,Marruecos ,Botany ,QK1-989 - Abstract
ABSTRACT: The specific parasite of Lactuca (Compositae) described in 2005 from the Iberian Peninsula as Phelipanche inexspectata (Orobanchaceae) and known so far in northeastern Spain and southern France, is shown to also occur in mountain areas of northern Africa, where it had been previously described under the neglected name Phelipanche cernua. Given the recent proposal to consider the aforementioned Lactuca parasite a mere variant of Phelipanche schultzii, we stress the neat differences between both species. RESUMEN: Phelipanche cernua Pomel (Orobanchaceae), un nombre prioritario para la especie del Mediterráneo Occidental recientemente descrita como Ph. inexspectata. Se dan pruebas de que la parásita específica de Lactuca (Compositae) descrita en 2005 de la Península Ibérica como Phelipanche inexspectata (Orobanchaceae), y conocida hasta ahora del noreste de España y el sur de Francia, alcanza las montañas del norte de África, de las que ya había sido descrita bajo el nombre Phelipanche cernua. Dada la reciente afirmación de que dicha parásita de Lactuca es una mera variante de Phelipanche schultzii, recalcamos las netas diferencias entre ambas especies.
- Published
- 2013
31. MT-HESS: an efficient Bayesian approach for simultaneous association detection in OMICS datasets, with application to eQTL mapping in multiple tissues
- Author
-
Lewin, Alex, Saadi, Habib, Peters, James E., Moreno-Moral, Aida, Lee, James C., Smith, Kenneth G. C., Petretto, Enrico, Bottolo, Leonardo, and Richardson, Sylvia
- Published
- 2016
- Full Text
- View/download PDF
32. Transcriptional Network Analysis for the Regulation of Left Ventricular Hypertrophy and Microvascular Remodeling
- Author
-
Moreno-Moral, Aida, Mancini, Massimiliano, D’Amati, Giulia, Camici, Paolo, and Petretto, Enrico
- Published
- 2013
- Full Text
- View/download PDF
33. Contribution to the flora of Asian and European countries : new national and regional vascular plant records, 9
- Author
-
Gonzalo Moreno-Moral, Mateusz Wolanin, Igor V. Kuzmin, Vladimir Mikhailovich Vasjukov, Adriano Stinca, Irina N. Egorova, Anna Wróbel, Gergely Király, Pavol Eliaš Jun, Renata Piwowarczyk, Giacomo Mei, Agnieszka Nobis, Denis A. Krivenko, Paweł Marciniuk, Nikita A. Vershinin, Beata Paszko, Marcin Nobis, Georgy A. Lazkov, Óscar Sánchez-Pedraja, Robert J. Soreng, Ewelina Klichowska, Arkadiusz Nowak, Jolanta Marciniuk, Marina V. Olonova, Nobis, M, Marciniuk, J, Marciniuk, P, Wolanin, M, Király, G, Nowak, A, Paszko, B, Klichowska, E, MORENO-MORAL, G, Piwowarczyk, R, SÁNCHEZ-PEDRAJA, Ó, Wróbel, A, Egorova, In, Eliaš, P JUN, Krivenko, Da, Kuzmin, Iv, Lazkov, Ga, Mei, G, Nobis, A, Olonova, Mv, Soreng, Rj, Stinca, A, Vasjukov, Vm, and Vershinin, Na
- Subjects
Chorology,taxonomy,native species,alien species,Asia,Europe ,Rubus phoenicolasius ,Solanum physalifolium ,Asia ,biology ,Melica ,native species ,alien species ,Plant Science ,biology.organism_classification ,Melica nutans ,Atraphaxis ,food.food ,Dichondra ,Europe ,Orobanche ,taxonomy ,Chorology ,food ,Botany ,Rubus ,chorology - Abstract
The paper presents new records for 39 vascular plant species from eight Eurasian countries. Aniselytron treutleri Poaceae , Hackelochloa granularis Poaceae , Melica kozlovii Poaceae and Melica nutans Poaceae are reported from China; Dichondra micrantha Convolvulaceae from Hungary; Orobanche serbica Orobanchaceae and Viscum album subsp. austriacum Santalaceae from Italy; Petrorhagia prolifera Caryophyllaceae , Puccinellia schischkinii and Stipa pulcherrima Poaceae from Kyrgyzstan; Megadenia speluncarum Brassicaceae , Phelipanche lavandulacea Orobanchaceae , Solanum physalifolium Solanaceae , Thymus lenensis Lamiaceae from Russia; Rubus phoenicolasius Rosaceae from Slovakia; Atraphaxis karataviensis Polygonaceae from Tajikistan; as well as Rubus austroslovacus and R. crispomarginatus Rosaceae in addition to Taraxacum acervatulum, T. aequilobum, T. amplum, T. ancistrolobum, T. bellicum, T. collarispinulosum, T. copidophyllum, T. corynodes, T. dentatum, T. gelertii, T. infuscatum, T. ingens, T. lucidum, T. paucilobum, T. plumbeum, T. portentosum, T. sinuatum, T. subhuelphersianum, T. telmatophilum, T. undulatiforme and T. undulatum Asteraceae from Ukraine. For each species synonyms, general distribution, habitat preferences, notes on taxonomy with remarks concerning recognition and distinction of the species from the most similar taxa occurring in a given country, as well as a list of recorded localities often far from the previously known areas are presented.
- Published
- 2020
34. Computational Tools for Accelerating Regenerative Medicine
- Author
-
Aida Moreno-Moral
- Subjects
Computer engineering ,Computer science ,Management of Technology and Innovation ,Biomedical Engineering ,High resolution ,Bioengineering ,Regenerative medicine ,Biotechnology ,Power (physics) - Published
- 2020
35. Multi-tissue analysis of co-expression networks by higher-order generalized singular value decomposition identifies functionally coherent transcriptional modules.
- Author
-
Xiaolin Xiao, Aida Moreno-Moral, Maxime Rotival, Leonardo Bottolo, and Enrico Petretto
- Subjects
Genetics ,QH426-470 - Abstract
Recent high-throughput efforts such as ENCODE have generated a large body of genome-scale transcriptional data in multiple conditions (e.g., cell-types and disease states). Leveraging these data is especially important for network-based approaches to human disease, for instance to identify coherent transcriptional modules (subnetworks) that can inform functional disease mechanisms and pathological pathways. Yet, genome-scale network analysis across conditions is significantly hampered by the paucity of robust and computationally-efficient methods. Building on the Higher-Order Generalized Singular Value Decomposition, we introduce a new algorithmic approach for efficient, parameter-free and reproducible identification of network-modules simultaneously across multiple conditions. Our method can accommodate weighted (and unweighted) networks of any size and can similarly use co-expression or raw gene expression input data, without hinging upon the definition and stability of the correlation used to assess gene co-expression. In simulation studies, we demonstrated distinctive advantages of our method over existing methods, which was able to recover accurately both common and condition-specific network-modules without entailing ad-hoc input parameters as required by other approaches. We applied our method to genome-scale and multi-tissue transcriptomic datasets from rats (microarray-based) and humans (mRNA-sequencing-based) and identified several common and tissue-specific subnetworks with functional significance, which were not detected by other methods. In humans we recapitulated the crosstalk between cell-cycle progression and cell-extracellular matrix interactions processes in ventricular zones during neocortex expansion and further, we uncovered pathways related to development of later cognitive functions in the cortical plate of the developing brain which were previously unappreciated. Analyses of seven rat tissues identified a multi-tissue subnetwork of co-expressed heat shock protein (Hsp) and cardiomyopathy genes (Bag3, Cryab, Kras, Emd, Plec), which was significantly replicated using separate failing heart and liver gene expression datasets in humans, thus revealing a conserved functional role for Hsp genes in cardiovascular disease.
- Published
- 2014
- Full Text
- View/download PDF
36. Notas breves
- Author
-
María Temes-Casas, Ángela Noguerol Seoane, Ana Rifon Lastra, Fernando Ortega González, Gema Parra Anguita, José Guerrero Ruiz, Consolación Fernández, Iñigo Sánchez, Mario Sanz Elorza, Eduardo Sobrino Vesperinas, José Ferrando Pla, Isabel Martínez, Gregorio Aragón, María José Fernández Lirola, Joaquín Molero Mesa, Gabriel Blanca, Rafael Rubio, Luis Balaguer, Esteban Manrique, María Ester Pérez, Pablo Vargas, José Luis Fernández-Alonso, Modesto Luceño, José Manuel Marín, Alfredo Barra Lázaro, Rodrigo Duno de Stefano, Manuel J. Macía, Santiago Castroviejo, Anabel Rial B., Leticia San Emeterio, Rita Yolanda Cavero, Luis G. Benavides, Adrián Escudero, José María Iriondo, Jorge Alfredo Reyes-Betancort, Ricardo González González, Gonzalo Gómez Casares, Manuel Laínz, Gonzalo Moreno Moral, Óscar Sánchez Pedraja, Ana Juan, Manuel B. Crespo, José Antonio García Trujillo, Andrés Revilla Onrubia, Silvia Romero Chala, Alberto González Talaván, and Luis Delgado
- Subjects
Botany ,QK1-989 - Published
- 2001
37. High-dose corticosteroid pulse therapy increases the survival rate in COVID-19 patients at risk of hyper-inflammatory response
- Author
-
Dionisio Guillamón-Fernández, Carolina Ramírez-Sánchez, María López-Vílchez, Miguel Ángel López Ruz, Justo Martínez-Colmenero, Alba María Castillo-Fernández, Francisco Andrés Padilla-Moreno, Aida Moreno-Moral, María Sánchez-Palop, Ana Ocaña-Granados, Miguel Ángel López Zúñiga, José Juan Moreno-Sánchez, Inmaculada Prior-Sánchez, María Amparo Pimentel-Villar, Sara Blázquez-Roselló, and Rosario Jódar-Moreno
- Subjects
Male ,Viral Diseases ,Epidemiology ,Anticoagulant Therapy ,030204 cardiovascular system & hematology ,ACE inhibitor therapy ,Biochemistry ,Gastroenterology ,Steroid Therapy ,Dexamethasone ,law.invention ,chemistry.chemical_compound ,Medical Conditions ,0302 clinical medicine ,Randomized controlled trial ,law ,Adrenal Cortex Hormones ,Medicine and Health Sciences ,030212 general & internal medicine ,Prospective Studies ,Prospective cohort study ,Virus Testing ,Multidisciplinary ,Pharmaceutics ,Cardiovascular therapy ,Middle Aged ,Hospitalization ,Survival Rate ,Chemistry ,Infectious Diseases ,Methylprednisolone ,Physical Sciences ,Medicine ,Corticosteroid ,Female ,Cytokine Release Syndrome ,Research Article ,Chemical Elements ,medicine.drug ,Adult ,medicine.medical_specialty ,medicine.drug_class ,Science ,Corticosteroid Therapy ,03 medical and health sciences ,Drug Therapy ,Diagnostic Medicine ,Internal medicine ,Lactate dehydrogenase ,medicine ,Humans ,Survival rate ,Aged ,Inflammation ,Ferritin ,business.industry ,Proportional hazards model ,SARS-CoV-2 ,Biology and Life Sciences ,Proteins ,Protein Complexes ,COVID-19 ,Covid 19 ,COVID-19 Drug Treatment ,Oxygen ,chemistry ,Spain ,Medical Risk Factors ,business - Abstract
Objective Test whether high dose corticosteroid pulse therapy (HDCPT) with either methylprednisolone or dexamethasone is associated with increased survival in COVID-19 patients at risk of hyper-inflammatory response. Provide some initial diagnostic criteria using laboratory markers to stratify these patients. Methods This is a prospective observational study, 318 met the inclusion criteria. 64 patients (20.1%) were treated with HDCPT by using at least 1.5mg/kg/24h of methylprednisolone or dexamethasone equivalent. A multivariate Cox regression (controlling for co-morbidities and other therapies) was carried out to determine whether HDCPT (among other interventions) was associated with decreased mortality. We also carried out a 30-day time course analysis of laboratory markers between survivors and non-survivors, to identify potential markers for patient stratification. Results HDCPT showed a statistically significant decrease in mortality (HR = 0.087 [95% CI 0.021–0.36]; P < 0.001). 30-day time course analysis of laboratory marker tests showed marked differences in pro-inflammatory markers between survivors and non-survivors. As diagnostic criteria to define the patients at risk of developing a COVID-19 hyper-inflammatory response, we propose the following parameters (IL-6 > = 40 pg/ml, and/or two of the following: C-reactive protein > = 100 mg/L, D-dimer > = 1000 ng/ml, ferritin > = 500 ng/ml and lactate dehydrogenase > = 300 U/L). Conclusions HDCPT can be an effective intervention to increase COVID-19 survival rates in patients at risk of developing a COVID-19 hyper-inflammatory response, laboratory marker tests can be used to stratify these patients who should be given HDCPT. This study is not a randomized clinical trial (RCT). Future RCTs should be carried out to confirm the efficacy of HDCPT to increase the survival rates of COVID-19.
- Published
- 2021
38. El máximo pluviométrico de los Montes de Pas: gradientes y nuevas estimaciones
- Author
-
Romero León, Fernando, Duque Urraca, Ángel, Carral Coo, José Manuel, Berasategi Lamas, José, Pombo Lavín, Ángela, Moreno Moral, Gonzalo, Rodríguez Velasco, Juan José, and Andrés Bravo, María Sol
- Subjects
Máximo pluviométrico ibérico ,Montes de Pas ,Cordillera Cantábrica - Abstract
La divisoria montañosa entre Cantabria y Burgos —con una altitud máxima de 1718 m—, en el encuentro de los ríos Pas, Miera y Asón —vertiente cantábrica— y Trueba —cuenca del Ebro— es reconocida como una de las áreas más lluviosas de la península ibérica, con una media anual de unos 3000 mm; estimación basada en las observaciones realizadas entre 1977 y 1996 en la cabecera del río Miera a 570 m de altitud que arroja un promedio de 2400 mm. El interés por cuantificar la lluvia caída en las zonas de montaña e identificar los máximos pluviométricos es un tema clásico de la climatología (Jansà, 1971). El máximo del que se ocupa esta colaboración, debido a la reciente incorporación de nuevas estaciones pluviométricas, adquiere mayor relevancia e implica la revisión de las antiguas aproximaciones. A pesar de las dificultades —relieve abrupto, alejamiento de poblaciones, intensidad de las nevadas—, el empeño e interés de entusiastas colaboradores y el apoyo del programa NATUREA han hecho posible la obtención de datos suficientes que permiten ya elevar la precipitación media anual en estas montañas, estimándola actualmente en unos 3500 mm considerando el óptimo pluviométrico hacia los 1300 m de altitud.
- Published
- 2021
39. Carduus carlinoides Gouan subsp. carlinoides (Asteraceae), taxon ajeno a la flora del Sistema Ibérico Septentrional
- Author
-
Alejandre Sáez, Juan Antonio, Arizaleta Urarte, José Antonio, Benito Ayuso, Javier, Moreno Moral, Gonzalo, Alejandre Sáez, Juan Antonio, Arizaleta Urarte, José Antonio, Benito Ayuso, Javier, and Moreno Moral, Gonzalo
- Abstract
Data are provided that definitively reject the presence of Carduus carliniodes Gouan subsp carlinoides in Northern Iberian System (Lo), supported by the author of the synthesis of Flora iberica on the basis of misinterpreted testimonies and herbarium specimens., Se aportan datos que desestiman definitivamente la presencia de Carduus carlinoides Gouan subsp carlinoides en Sistema Ibérico Septentrional (La Rioja), sostenida por el autor de la síntesis de Flora iberica sobre la base de testimonios y pliegos de herbario mal interpretados.
- Published
- 2021
40. WWP2 regulates pathological cardiac fibrosis by modulating SMAD2 signaling
- Author
-
Cristina Beltrami, Owen J. L. Rackham, Costanza Emanueli, Nithya Devapragash, Wei-Ping Yu, Xi-Ming Sun, Stuart A. Cook, Norbert Hubner, Francesco Pesce, Ee Ling Heng, Elisabeth Tan, Aida Moreno-Moral, Enrique Lara-Pezzi, Gianni D Angelini, Enrico Petretto, Huimei Chen, Paul J.R. Barton, Nathan Harmston, Michal Pravenec, Sebastian Schafer, Maxime Rotival, Nicole Tee, Massimilano Mancini, Leanne E. Felkin, Leonardo Bottolo, Prashant K. Srivastava, Kirill Shkura, Cardiovascular and Metabolic Disorders [Singapor] (cvmd), Duke-NUS Medical School [Singapore], Università degli studi di Bari Aldo Moro = University of Bari Aldo Moro (UNIBA), Ospedale San Giovanni di Dio, Firenze, National Heart and Lung Institute [London] (NHLI), Imperial College London-Royal Brompton and Harefield NHS Foundation Trust, Génétique Evolutive Humaine - Human Evolutionary Genetics, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Faculty of Medicine [London, UK], Imperial College London, Agency for science, technology and research [Singapore] (A*STAR), Institute of Molecular and Cell Biology, National University of Singapore (NUS)-Agency for science, technology and research [Singapore] (A*STAR), MRC London Institute of Medical Sciences (LMC), National Heart Centre Singapore (NHCS), Royal Brompton and Harefield NHS Foundation Trust, Cardiovascular Research Centre [London], Centro Nacional de Investigaciones Cardiovasculares Carlos III [Madrid, Spain] (CNIC), Instituto de Salud Carlos III [Madrid] (ISC), University of Bristol [Bristol], Institute of Physiology [Prague], Czech Academy of Sciences [Prague] (CAS), University of Cambridge [UK] (CAM), The Alan Turing Institute, MRC Biostatistics Unit [Cambridge, UK], Max Delbrück Center for Molecular Medicine [Berlin] (MDC), Helmholtz-Gemeinschaft = Helmholtz Association, German Center for Cardiovascular Research (DZHK), Berlin Institute of Health (BIH), Charité - UniversitätsMedizin = Charité - University Hospital [Berlin], The research was primarily supported by National Medical Research Council (NMRC) Singapore grant NMRC/CBRG/0106/2016 (to E.P.) and the British Heart Foundation (BHF) Ph.D. Studentship grant FS/11/25/28740 (to E.P). We acknowledge additional funding support from European Union FP7 CardioNeT-ITN-289600 (to E.L.-P., S.A.C., and P.J.R.B.), Heart Research UK (to P.J.R.B.), NIHR CV BRU of Royal Brompton and Harefield, NHS Foundation Trust (to S.A.C. and P.J.R.B.), BHF (to S.A.C.), Leducq Foundation (to S.A.C.), MRC UK (to S.A.C.), BHF Program Grant no. RG/15/5/31446 (to C.E. and E.P.). M.P. was supported by Praemium Academiae award of the Czech Academy of Sciences and grant 14-36804G from the Czech Science Foundation., We wish to thank Dr. Jacques Behmoaras for contributing critical and constructive comments to the manuscript., European Project: 289600,EC:FP7:PEOPLE,FP7-PEOPLE-2011-ITN,CARDIONET(2012), Moreno-Moral, Aida [0000-0002-8155-3146], Barton, Paul J. R. [0000-0002-1165-7767], Schafer, Sebastian [0000-0002-6909-8275], Bottolo, Leonardo [0000-0002-6381-2327], Cook, Stuart A. [0000-0001-6628-194X], Petretto, Enrico [0000-0003-2163-5921], Apollo - University of Cambridge Repository, National Medical Research Council (Singapur), British Heart Foundation, Unión Europea. Comisión Europea, National Institutes of Health (Estados Unidos), Czech Science Foundation, Fondation Leducq, NHS Foundation Trusth, University of Bari Aldo Moro (UNIBA), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Leducq Foundation for Cardiovascular Research, Commission of the European Communities, Heart Research UK, Medical Research Council (MRC), Barton, Paul JR [0000-0002-1165-7767], and Cook, Stuart A [0000-0001-6628-194X]
- Subjects
Male ,45/43 ,BLOOD-PRESSURE ,02 engineering and technology ,MESH: Protein Isoforms ,MESH: Heart Diseases / genetics ,Mice ,Transforming Growth Factor beta ,42/44 ,Fibrosis ,Protein Isoforms ,Medicine ,MESH: Animals ,lcsh:Science ,MESH: Ubiquitin-Protein Ligases / genetics ,MESH: Fibrosis / metabolism ,MESH: Genetic Predisposition to Disease* / genetics ,GENE-EXPRESSION ,Regulation of gene expression ,Extracellular Matrix Proteins ,MESH: Middle Aged ,MESH: Fibrosis / genetics ,TGF-BETA ,MESH: Heart Diseases / metabolism ,MESH: Ubiquitin-Protein Ligases / metabolism ,3. Good health ,Cell biology ,Ubiquitin ligase ,FIBROBLAST ,MESH: Young Adult ,Science & Technology - Other Topics ,64/60 ,Cardiomyopathies ,0210 nano-technology ,Cardiac function curve ,Science ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,REVEALS ,Humans ,Aged ,MESH: Adolescent ,Science & Technology ,MESH: Humans ,[SDV.GEN.GPO]Life Sciences [q-bio]/Genetics/Populations and Evolution [q-bio.PE] ,IDENTIFICATION ,MESH: Smad2 Protein / metabolism ,MESH: Extracellular Matrix Proteins / metabolism ,MESH: Adult ,MESH: Cardiomyopathies / metabolism ,medicine.disease ,030104 developmental biology ,Cardiovascular and Metabolic Diseases ,E3 UBIQUITIN LIGASE ,RAT ,DETERMINANT ,lcsh:Q ,Myocardial fibrosis ,MESH: Female ,0301 basic medicine ,Cardiac fibrosis ,General Physics and Astronomy ,Smad2 Protein ,Gene Regulatory Networks ,13/89 ,MESH: Aged ,MESH: Transforming Growth Factor beta / metabolism ,Multidisciplinary ,biology ,MESH: Smad2 Protein / genetics ,article ,Middle Aged ,MESH: Cardiomyopathies / genetics ,021001 nanoscience & nanotechnology ,692/4019/592/75/74 ,MESH: Gene Expression Regulation ,Multidisciplinary Sciences ,Centre for Surgical Research ,HEART-FAILURE ,Female ,38/39 ,Adult ,Adolescent ,Heart Diseases ,MESH: Mice, Transgenic ,Ubiquitin-Protein Ligases ,Mice, Transgenic ,14/32 ,82/80 ,Young Adult ,Animals ,Genetic Predisposition to Disease ,14/35 ,MESH: Mice ,Pressure overload ,business.industry ,General Chemistry ,MESH: Male ,Gene Expression Regulation ,[SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics ,Ubiquitin ligases ,Heart failure ,13/51 ,biology.protein ,631/45/474/2073 ,MESH: Gene Regulatory Networks ,business - Abstract
Cardiac fibrosis is a final common pathology in inherited and acquired heart diseases that causes cardiac electrical and pump failure. Here, we use systems genetics to identify a pro-fibrotic gene network in the diseased heart and show that this network is regulated by the E3 ubiquitin ligase WWP2, specifically by the WWP2-N terminal isoform. Importantly, the WWP2-regulated pro-fibrotic gene network is conserved across different cardiac diseases characterized by fibrosis: human and murine dilated cardiomyopathy and repaired tetralogy of Fallot. Transgenic mice lacking the N-terminal region of the WWP2 protein show improved cardiac function and reduced myocardial fibrosis in response to pressure overload or myocardial infarction. In primary cardiac fibroblasts, WWP2 positively regulates the expression of pro-fibrotic markers and extracellular matrix genes. TGFβ1 stimulation promotes nuclear translocation of the WWP2 isoforms containing the N-terminal region and their interaction with SMAD2. WWP2 mediates the TGFβ1-induced nucleocytoplasmic shuttling and transcriptional activity of SMAD2., Pathological cardiac fibrosis is a hallmark of diseases leading to heart failure. Here, the authors used systems genetics to identify a pro-fibrotic gene network regulated by WWP2, a E3 ubiquitin ligase, which orchestrates the nucleocytoplasmic shuttling and transcriptional activity of SMAD2 in the diseased heart.
- Published
- 2019
41. Orobanche centaurina Bertol. the correct name for O. kochii F.W. Schultz (Orobanchaceae)
- Author
-
Zázvorka, Ji?í, Sánchez Pedraja, Oscar, Moreno Moral, Gonzalo, Carlón Ruiz, Luis, Domina, Gianniantonio, Laínz Gallo, Manuel, Piwowarczyk, Renata, and Jiří ZÁZVORKA, Óscar SÁNCHEZ PEDRAJA, Gonzalo MORENO MORAL, Luis CARLÓN RUIZ, Gianniantonio DOMINA, Manuel LAÍNZ GALLO, Renata PIWOWARCZYK
- Subjects
plantas parásitas ,Orobanchaceae ,nomenclatura ,Settore BIO/02 - Botanica Sistematica ,Settore BIO/03 - Botanica Ambientale E Applicata ,UNESCO::CIENCIAS DE LA VIDA ,Eurasian flora, Nomenclature, Orobanchaceae, Parasitic plants ,CIENCIAS DE LA VIDA [UNESCO] ,flora euroasiática - Abstract
After the studying the original material of Orobanche centaurina Bertol. (Orobanchaceae) deposited in BOLO, a forgotten species described from Massa (Central Italy) parasitizing Centaurea paniculata L., and compared it with the type of O. kochii F.W. Schultz and with the types of others taxa actually considered synonyms of this, we concluded that the name of Bertoloni is the correct one for this species. Orobanche centaurina Bertol., nombre correcto para O. kochii F.W. Schultz (Orobanchaceae). Después de estudiar el material original de Orobanche centaurina Bertol. (Orobanchaceae) depo sitado en BOLO, una olvidada especie descrita de Massa (Italia central) parásita de Centaurea paniculata L., y com pararlo con el tipo de O. kochii F.W. Schultz y con los tipos de otros táxones actualmente considerados sinónimos de éste, estimamos que el nombre de Bertoloni es el correcto y prioritario para esta especie
- Published
- 2019
42. High-Dose Corticosteroid Pulse Therapy Increases the Survival Rate in COVID-19 Patients at Risk of Cytokine Storm
- Author
-
López-Vílchez M, Ocaña-Granados A, Sanchez-Palop M, Moreno-Moral A, López-Zúñiga Má, Blázquez-Roselló S, Ruz Mal, Jódar-Moreno R, Padilla-Moreno F, Castillo-Fernández Am, Martínez-Colmenero J, Prior-Sánchez I, Guillamón-Fernández D, Pimentel-Villar Ma, Ramírez-Sánchez C, and Moreno-Sánchez Jj
- Subjects
medicine.medical_specialty ,medicine.drug_class ,business.industry ,Proportional hazards model ,Mortality rate ,Odds ratio ,medicine.disease ,Methylprednisolone ,Internal medicine ,Cohort ,medicine ,Corticosteroid ,business ,Cytokine storm ,Survival rate ,medicine.drug - Abstract
Background: COVID-19 is a disease caused by SARS-CoV-2, which can develop into a hyper-inflammatory late phase or “cytokine storm” that poses high risk of death. It has been proposed that the management of this cytokine storm is crucial to increase the survival rate of COVID-19 patients. However, to date there are no diagnostic criteria based on inflammatory markers that could be used to select the COVID-19 patients at risk of developing a cytokine storm or effective interventions that can decreased mortality in these patients. Methods: We carried out a quasi-experimental study to test whether high dose corticosteroid pulse therapy with either dexametaxona or methylprednisolone were associated with reduced mortality in patients at risk of cytokine storm and assess which laboratory markers can be used to pre-select these patients. To control the development and progression of this hyper-inflammtory phase, 64 patients (20.1%) were treated with high dose corticosteroid pulse therapy (HDCPT) by using either dexametaxona or methylprednisolone daily for 2-5 days at doses of at least 30mg or 125mg respectively. To determine diagnostic criteria that can be used to defined patients at risk of cytokine storm, we carried out a 30-day time course analysis of laboratory markers between survivors and non-survivors. A multivariate Cox regression (controlling for co-morbidities and therapies) was carried out to determine whether HDCPT (among other interventions) was associated with decreased mortality in both the full cohort, and in a subgroup containing the patients with the derived criteria. Findings: Out of 500 patients originally recruited, 381 met the inclusion criteria of SARS-COV-19 detection by PCR or serology (n=272, 71.2%) or high clinical suspicion (n= 46, 16.9 %), defined as having bilateral pulmonary infiltrate or lymphopenia with according clinical profile. HDCPT was the only intervention that showed a significant decrease in mortality (odds ratio = 0.093 [95% CI 0.023 – 0.38]; P = 40 pg/ml and/or two of the following: C-reactive protein >= 100 mg/L, D-dimer >= 1000 ng/ml, ferritin >= 500 ng/ml and lactate dehydrogenase >= 300 U/L. Multivariate Cox regression by pre-selecting the patients using this criteria evidenced also a decreased mortality rate with HDCPT with a lower odds ratio (odds ratio = 0.081 [95% CI 0.011 – 0.56]; P = 0.011). Conclusions: Preventing and/or controlling the development of the cytokine storm in patients at high-inflammatory risk with HDCPT is a widely available therapy to increase survival rate in these patients. We estimate a 14.2% [95% CI 0.792 - 0.975] increase of survival rate. We also suggest some initial clinical variables that can aid with the identification of the patients who will benefit from this intervention. Funding Statement: There were no funding sources in this study. Declaration of Interests: The authors declare no competing interests. Ethics Approval Statement: This study was approved by the Ethics Committee of the Complejo Hospitalario de Jaen (Hospital of Jaen), Spain (0946-N-20). According to the local ethics committee regulations, verbal consent was obtained from all the patients that joined the study.
- Published
- 2020
43. Association Between Baseline Circulating Tumor Cells, Molecular Tumor Profiling, and Clinical Characteristics in a Large Cohort of Chemo-naive Metastatic Colorectal Cancer Patients Prospectively Collected
- Author
-
Javier Sastre, Milagros Balbín, Guillermo Quintero, Virginia Moreno Moral, Virginia de la Orden, Antonio Martínez, Beatriz Mediero, Inmaculada Bando, Patricia Llovet, Enrique Aranda, Maria José Ortiz-Morales, Sarai Palanca, Pilar García-Alfonso, Jose María Vieitez, Antonieta Salud, Silvia Gil Calle, Beatriz Bellosillo, Carlos F. Lopez, Eduardo Díaz-Rubio, and Maria Isabel Peligros Gomez
- Subjects
Oncology ,Male ,Lung Neoplasms ,Organoplatinum Compounds ,FOLFIRINOX ,Colorectal cancer ,DNA Mutational Analysis ,Leucovorin ,Cell Count ,Metastasis ,0302 clinical medicine ,Carcinoembryonic antigen ,Circulating tumor cell ,CEA ,FOLFOX ,Antineoplastic Combined Chemotherapy Protocols ,Prospective Studies ,biology ,Liver Neoplasms ,Gastroenterology ,Mutational status ,Middle Aged ,Neoplastic Cells, Circulating ,Prognosis ,Progression-Free Survival ,Bevacizumab ,Oxaliplatin ,030220 oncology & carcinogenesis ,FOLFIRI ,030211 gastroenterology & hepatology ,Female ,Microsatellite Instability ,Fluorouracil ,Colorectal Neoplasms ,medicine.drug ,Adult ,Proto-Oncogene Proteins B-raf ,medicine.medical_specialty ,Adolescent ,Bone Neoplasms ,Irinotecan ,Risk Assessment ,BRAF ,03 medical and health sciences ,Young Adult ,Internal medicine ,medicine ,Biomarkers, Tumor ,Humans ,neoplasms ,Aged ,Neoplasm Staging ,Performance status ,business.industry ,medicine.disease ,digestive system diseases ,Gene expression profiles ,Mutation ,biology.protein ,ras Proteins ,Microsatellite instability ,Camptothecin ,business - Abstract
This is a post hoc analysis of biomarkers from a large cohort of patients with chemo-naive metastatic colorectal cancer. Both high baseline circulating tumor cell count and RAS mutated were associated with clinical or pathologic features classically associated with poor prognosis. Selection of high- and low-risk populations may help to individualize approaches in the future. Background: Clinicopathologic characteristics and prognostic and predictive factors offer valuable guidance when selecting optimal first-line treatment in patients with metastatic colorectal cancer (CRC). The association between baseline circulating tumor cell (bCTC) count, molecular tumor profile, and clinicopathologic features was analyzed in a chemo-naive metastatic CRC population. Patients and Methods: A total of 1202 patients from the Spanish VISNU-1 (FOLFIRINOX/bevacizumab vs. FOLFOX/bevacizumab) and VISNU-2 (FOLFIRI/bevacizumab vs. FOLFIRI/cetuximab; RAS-wildtype) studies were analyzed for mutational status and bCTC count. The association between clinicopathologic characteristics and bCTC count, mutational status, and microsatellite instability (MSI) was analyzed in 589 eligible patients. Results: Interestingly, 41% of the population studied presented >= 3 bCTC count. bCTC count >= 3 was associated with worse performance status (according Eastern Cooperative Oncology Group scale), stage IV at diagnosis, at least 3 metastatic sites, and elevated carcinoembryonic antigen (CEA) levels; but not with RAS or BRAF mutations or high MSI. BRAFmut (BRAF mutated) tumors were associated with right-sided primary tumors, peritoneum, distant lymph node metastasis, and less frequent liver involvement. RASmut (RAS mutated) was associated with worse performance status; stage IV at diagnosis; right-sided primary tumors; liver, lung, and bone metastases; at least 3 metastatic sites; and elevated CEA, whereas PIK3CAmut (PIK3CA mutated) tumors were associated with right-sided primary tumors, high CEA serum levels, and older age. High MSI was associated with right-sided primary tumors, distant lymph nodes metastasis, and lower CEA levels. Conclusions: In our study, elevated bCTCs and RASmut were associated with clinicopathologic features known to be associated with poor prognosis; whereas the poor prognosis of BRAFmut tumors in chemo-naive metastatic CRC is not explained by associations with poor clinicopathologic prognostic factors, except right-sided primary tumors. (C) 2020 Elsevier Inc. All rights reserved.
- Published
- 2020
44. Phelipanche zangezuri (Orobanchaceae), a New Species from the Caucasus, Armenia
- Author
-
Narine Zakaryan, Óscar Sánchez-Pedraja, Nune Kartashyan, Gonzalo Moreno-Moral, and Renata Piwowarczyk
- Subjects
0106 biological sciences ,Caesia ,geography ,geography.geographical_feature_category ,biology ,Plant Science ,biology.organism_classification ,010603 evolutionary biology ,01 natural sciences ,Pulchella ,Orobanchaceae ,Botany ,Tragopogon pterocarpus ,Ecology, Evolution, Behavior and Systematics ,Mountain range ,010606 plant biology & botany - Abstract
Phelipanche zangezuri Piwow., O. Sanchez & Moreno Mor. sp. nova (Orobanchaceae) is described from the Lesser Caucasus in Armenia. The species grows on a rocky slope near roadside in the Zangezur mountain range, 2300 m a.s.l., and parasitises Tragopogon pterocarpus. We present detailed description and illustrations, and also a comparison with the morphologically closest P. pulchella, P. hajastanica and P. caesia.
- Published
- 2018
45. High-dose corticosteroid pulse therapy increases the survival rate in COVID-19 patients at risk of hyper-inflammatory response
- Author
-
López Zúñiga, Miguel Ángel, primary, Moreno-Moral, Aida, additional, Ocaña-Granados, Ana, additional, Padilla-Moreno, Francisco Andrés, additional, Castillo-Fernández, Alba María, additional, Guillamón-Fernández, Dionisio, additional, Ramírez-Sánchez, Carolina, additional, Sanchez-Palop, María, additional, Martínez-Colmenero, Justo, additional, Pimentel-Villar, María Amparo, additional, Blázquez-Roselló, Sara, additional, Moreno-Sánchez, José Juan, additional, López-Vílchez, María, additional, Prior-Sánchez, Inmaculada, additional, Jódar-Moreno, Rosario, additional, and López Ruz, Miguel Ángel, additional
- Published
- 2021
- Full Text
- View/download PDF
46. Contribution to the flora of Asian and European countries: new national and regional vascular plant records, 9
- Author
-
NOBIS, Marcin, primary, MARCINIUK, Jolanta, additional, MARCINIUK, Paweł, additional, WOLANIN, Mateusz, additional, KIRÁLY, Gergely, additional, NOWAK, Arkadiusz, additional, PASZKO, Beata, additional, KLICHOWSKA, Ewelina, additional, MORENO-MORAL, Gonzalo, additional, PIWOWARCZYK, Renata, additional, SÁNCHEZ-PEDRAJA, Óscar, additional, WRÓBEL, Anna, additional, EGOROVA, Irina N., additional, JUN, Pavol Eliaš, additional, KRIVENKO, Denis A., additional, KUZMIN, Igor V., additional, LAZKOV, Georgy A., additional, MEI, Giacomo, additional, NOBIS, Agnieszka, additional, OLONOVA, Marina V., additional, SORENG, Robert J., additional, STINCA, Adriano, additional, VASJUKOV, Vladimir M., additional, and VERSHININ, Nikita A., additional
- Published
- 2020
- Full Text
- View/download PDF
47. Computational Tools for Accelerating Regenerative Medicine
- Author
-
Moreno-Moral, Aida, primary
- Published
- 2020
- Full Text
- View/download PDF
48. In Vivo Generation of Post-infarct Human Cardiac Muscle by Laminin-Promoted Cardiovascular Progenitors
- Author
-
Yap, Lynn, primary, Wang, Jiong-Wei, additional, Moreno-Moral, Aida, additional, Chong, Li Yen, additional, Sun, Yi, additional, Harmston, Nathan, additional, Wang, Xiaoyuan, additional, Chong, Suet Yen, additional, Vanezis, Konstantinos, additional, Öhman, Miina K., additional, Wei, Heming, additional, Bunte, Ralph, additional, Gosh, Sujoy, additional, Cook, Stuart, additional, Hovatta, Outi, additional, de Kleijn, Dominique P.V., additional, Petretto, Enrico, additional, and Tryggvason, Karl, additional
- Published
- 2020
- Full Text
- View/download PDF
49. High-Dose Corticosteroid Pulse Therapy Increases the Survival Rate in COVID-19 Patients at Risk of Cytokine Storm
- Author
-
López-Zúñiga, Miguel Ángel, primary, Moreno-Moral, Aida, additional, Ocaña-Granados, Ana, additional, Padilla-Moreno, Francisco, additional, Castillo-Fernández, Alba María, additional, Guillamón-Fernández, Dionisio, additional, Sanchez-Palop, María, additional, Martínez-Colmenero, Justo, additional, Pimentel-Villar, María Amparo, additional, Blázquez-Roselló, Sara, additional, Moreno-Sánchez, José Juan, additional, López-Vílchez, Maria, additional, Prior-Sánchez, Inmaculada, additional, Jódar-Moreno, Rosario, additional, Ramírez-Sánchez, Carolina, additional, and López Ruz, Miguel Angel, additional
- Published
- 2020
- Full Text
- View/download PDF
50. Phelipanche hajastanica (Orobanchaceae), a New Species from Armenia
- Author
-
Siranush G. Nanagulyan, Gonzalo Moreno-Moral, Nune Kartashyan, Renata Piwowarczyk, Narine Zakaryan, and Óscar Sánchez-Pedraja
- Subjects
0106 biological sciences ,Caesia ,geography ,geography.geographical_feature_category ,biology ,Ecology ,Plant Science ,biology.organism_classification ,010603 evolutionary biology ,01 natural sciences ,Pulchella ,Orobanchaceae ,Nepeta ,Botany ,Ecology, Evolution, Behavior and Systematics ,Mountain range ,010606 plant biology & botany - Abstract
Phelipanche hajastanica Piwow., O. Sanchez & Moreno Mor., sp. nova (Orobanchaceae) is described from the Lesser Caucasus in the southeastern part of Armenia. The species grows in two small gorges in the Vayk mountain range, and parasitises Nepeta trautvetteri, a Transcaucasian endemic. A detailed description and illustrations are provided. We compare P. hajastanica with the morphologically most similar species P. pulchella, P. bungeana, and P. caesia, of which the first two are lectotypified here.
- Published
- 2017
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.