58 results on '"Moreira LS"'
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2. EFFECT OF BONE MARROW HEMATOPOIETIC PROGENITOR CELLS CRYOPRESERVATION ON TRANSPLANTATION OUTCOMES
- Author
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Belisário, AR, primary, Moreira, LS, additional, Costa, LA, additional, Martins, MC, additional, Andrade, RK, additional, Pederzoli, PRMP, additional, and Prata, KL, additional
- Published
- 2023
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3. Respiratory viruses in children younger than five years old with acute respiratory disease from 2001 to 2004 in Uberlândia, MG, Brazil
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Costa, LF, Yokosawa, J, Mantese, OC, Oliveira, TFM, Silveira, HL, Nepomuceno, LL, Moreira, LS, Dyonisio, G, Rossi, LMG, Oliveira, RC, Ribeiro, LZG, and Queiróz, DAO
- Subjects
reverse transcription of polymerase chain reaction ,children ,viruses ,respiratory viruses ,virus diseases ,immunofluorescence assay ,respiratory disease - Abstract
The main viruses involved in acute respiratory diseases among children are: respiratory syncytial virus (RSV), influenzavirus (FLU), parainfluenzavirus (PIV), adenovirus (AdV), human rhinovirus (HRV), and the human metapneumovirus (hMPV). The purpose of the present study was to identify respiratory viruses that affected children younger than five years old in Uberlândia, Midwestern Brazil. Nasopharyngeal aspirates from 379 children attended at Hospital de Clínicas (HC/UFU), from 2001 to 2004, with acute respiratory disease, were collected and tested by immunofluorescence assay (IFA) to detect RSV, FLU A and B, PIV 1, 2, and 3 and AdV, and RT-PCR to detect HRV. RSV was detected in 26.4% (100/379) of samples, FLU A and B in 9.5% (36/379), PIV 1, 2 and 3 in 6.3% (24/379) and AdV in 3.7% (14/379). HRV were detected in 29.6% (112/379) of the negative and indeterminate samples tested by IFI. RSV, particularly among children less than six months of life, and HRV cases showed highest incidence. Negative samples by both IFA and RT-PCR might reflect the presence of other pathogens, such as hMPV, coronavirus, and bacteria. Laboratorial diagnosis constituted an essential instrument to determine the incidence of the most common viruses in respiratory infections among children in this region.
- Published
- 2006
4. IDENTIFICATION OF DIFFERENTIALLY EXPRESSED GENES INDUCED BY HYDROXYUREA IN RETICULOCYTES FROM SICKLE CELL ANAEMIA PATIENTS
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Moreira, LS, primary, De Andrade, TG, additional, Albuquerque, DM, additional, Cunha, AF, additional, Fattori, A, additional, Saad, STO, additional, and Costa, FF, additional
- Published
- 2008
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5. Respiratory viruses in children younger than five years old with acute respiratory disease from 2001 to 2004 in Uberlândia, MG, Brazil
- Author
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Costa, LF, primary, Yokosawa, J, additional, Mantese, OC, additional, Oliveira, TFM, additional, Silveira, HL, additional, Nepomuceno, LL, additional, Moreira, LS, additional, Dyonisio, G, additional, Rossi, LMG, additional, Oliveira, RC, additional, Ribeiro, LZG, additional, and Queiróz, DAO, additional
- Published
- 2006
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6. Toxicity of REEs, Th, and U: A Biodisponibility, Cytotoxicity, and Bioaccumulation Assessment in Marine Sediment.
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Cordeiro SG, Pereira ACH, Endringer DC, Moreira LS, Carneiro MTWD, de Souza JR, and Brandão GP
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In this work, bioaccessibility tests for rare earth elements (REEs), Th, and U in marine sediment were carried out, in addition to complementary tests for cytotoxicity and bioaccumulation for the elements La, Ce, Eu, and Gd. The evaluation of human health risk through dermal absorption and oral ingestion was performed using the hazard quotient (HQ). According to the gastric digestion simulation (SBET), it was observed that the elements Ce and Nd exhibited higher absorption capacities in the human body (> 2 µg g
-1 ). La and Sc presented intermediate concentrations (close to 1 µg g-1 ), while the remaining elements displayed concentrations below 0.5 µg g-1 . In the gastrointestinal digestion extraction stage (PBET), all the elements maintained a similar absorption capacity to that observed in SBET, except for the absorption of Y which increased. The results of the bioaccumulation test conducted with fibroblast cells (L929) indicated that La and Eu had a 25% probability of intracellular accumulation. The cell viability test, with exposure to a standard REEs, Th, and U solution in 2% v v-1 HNO3 medium (until 100 μg mL-1 ) and an aqueous solution of La2 O3 , Gd(NO3 )3 , Ce(NO3 )3 , and Eu2 O3 (until 1000 μg mL-1 ), did not demonstrate cytotoxic effects on fibroblast cells. Considering the ingestion hazard quotient (HQing ) and dermal hazard quotient (HQderm ) obtained, it was suggested that there is no significant risk of non-carcinogenic effects (< 1). However, they had higher HQing values compared to HQderm , indicating that REEs pose more significant risk to human health through oral ingestion absorption than dermal absorption., (© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)- Published
- 2024
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7. Distribution of acid-volatile sulfides and simultaneously extracted metals in Guanabara Bay: combination of anthropogenic, sedimentological, and geochemical processes.
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Cordeiro RC, Santelli RE, Valle Machado WT, Gomes AS, Moreira LS, Oliveira RL, Abreu IM, and de Fatima Guadalupe Meniconi M
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- Acids, Bays, Environmental Monitoring, Geologic Sediments chemistry, Metals analysis, Sulfides chemistry, Metals, Heavy analysis, Trace Elements, Water Pollutants, Chemical analysis
- Abstract
Coastal sedimentary systems are affected by continental and marine metal pollutant inputs associated with different hydrodynamic characteristics and geochemical processes. These include the formation of acid-volatile sulfides (AVS) within sediments, which affects metal bioavailability and associated aquatic biota toxicity risks. Physicochemical changes in these environments in the face of extreme natural or man-made environmental influences can dramatically alter metal bioavailability and toxicity through metal binding and immobilization as insoluble sulfides. Surface sediments from Guanabara Bay, river mouths, and two mangrove areas were collected, and AVS and simultaneously extracted metals Cd, Cu, Fe, Mn, Ni, Pb, and Zn and ΣSEM were determined to assess sediment quality. A severe eutrophication history favored AVS concentrations exceeding or close to the sum-SEM concentrations, demonstrating that AVS play an important role in making trace metals unavailable for assimilation by living organisms, mitigating the risks of contamination for the local biota. This eutrophication-driven sulfide accumulation may attenuate the sediment toxicity in sites heavily polluted by metals, while some fewer eutrophic sites became more exposed to metals in excess to AVS., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
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- 2024
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8. Microwave-assisted extraction based on emulsion breaking with natural deep eutectic solvent for vegetable oil sample preparation prior to elemental determination by ICP OES.
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Costa FS, Moreira LS, Ludovico LL, Volpe J, de Oliveira AC, Dos Santos MP, da Silva EGP, Souto DEP, Grassi MT, Gonzalez MH, and Amaral CDB
- Abstract
This study presents a novel and efficient method for the extraction of Al, Ca, Cr, Cu, K, Mg, Mn, and Zn in vegetable oil samples using a Natural Deep Eutectic Solvent (NADES) as an extractor combined with microwave radiation (MW) in an emulsion system. The NADES prepared with choline chloride:oxalic acid:water (1:1:4 molar ratio) provided a high extraction rate using 5.0 mL of the sample, 1.7 mL of NADES, and 1.3 mL of Triton X-100. The optimum conditions were obtained with 36 s of vortexing, 5 min of extraction, and 10 s for emulsion-breaking in MW. Under these conditions, recoveries ranged from 91% to 110% and relative standard deviations <9.0% were obtained. The limit of quantification (mg kg
-1 ) was: 0.018 (Al), 0.032 (Ca), 0.007 (Cr), 0.006 (Cu), 0.013 (K), 0.027 (Mg), 0.002 (Mn), and 0.019 (Zn). The proposed method showed comparable results to reference methods and advantages, such as speed, low cost, and simplicity. The combination of NADES and MW represents a sustainable and innovative approach to the elemental determination composition of vegetable oils and contributes to advances in sample preparation methods., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper, (Copyright © 2023. Published by Elsevier B.V.)- Published
- 2024
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9. L-glutathione 1% promotes neuroprotection of nitrergic neurons and reduces the oxidative stress in the jejunum of rats with Walker-256-bearing tumor.
- Author
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de Oliveira AP, Perles JVCM, de Souza SRG, Sestak SS, da Motta Lima FG, Almeida GHDR, Cicero LR, Clebis NK, Guarnier FA, Blegniski FP, Vasconcelos RC, Araújo AA, Comar JF, Moreira LS, Sehaber-Sierakowski CC, Zanoni KPS, and Zanoni JN
- Subjects
- Rats, Animals, Jejunum, Rats, Wistar, Neuroprotection, Oxidative Stress, Glutathione metabolism, Myenteric Plexus pathology, Nitrergic Neurons, Neoplasms metabolism, Neoplasms pathology
- Abstract
Aims: Our main goals were to investigate the effects of L-glutathione (1%) treatment in Walker-256 tumor-bearing rats by analyzing immunoreactive neurons (IR), responsive to the nNOS enzyme and 3-Nitrotyrosine, in their jejunum myenteric plexus. Moreover, the oxidative state and inflammatory process in these animals were investigated., Methods: Four experimental groups were utilized: control (C), control treated with L-glutathione (CGT), Walker-256 tumor-bearing rats (TW), and Walker-256 tumor-bearing rats treated with L-glutathione (TWGT). After 14 days of tumor inoculation, the jejunum was collected for immunohistochemical techniques and assessment of oxidative status. Plasma was collected to evaluate oxidative status and measure cytokines., Results: The TW group exhibited a decrease of reduced glutathione in their jejunum, which was prevented in the L-glutathione treated TWGT group. TW animals presented pronounced oxidative stress by increasing levels of lipoperoxidation in their jejunum and malondialdehyde in their plasma; however, the L-glutathione treatment in TWGT group was not able to avoid it. The total antioxidant capacity was altered in groups TW and TWGT, yet the last one had a better index in their plasma. The IL-10, and TNF-α levels increased in TWGT animals. The nNOS-IR neuron density decreased in the jejunum myenteric plexus of the TW group, which was avoided in the TWGT group. The nNOS +3-Nitrotyrosine neurons quantification did not show significative alterations., Conclusion: The treatment with L-glutathione (1%) imposed an important defense to some parameters of oxidative stress induced by TW-256, leading to neuroprotection to the loss in the nNOS-IR neuron density., (© 2023 John Wiley & Sons Ltd.)
- Published
- 2023
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10. Diversity of Microbiomes Across a 13,000-Year-Old Amazon Sediment.
- Author
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Thompson CC, Tschoeke D, Coutinho FH, Leomil L, Garcia GD, Otsuki K, Turcq BJ, Moreira LS, Turcq PFM, Cordeiro RC, Asp NE, and Thompson FL
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- Bacteria, Metagenome, Rivers microbiology, Lakes microbiology, Geologic Sediments microbiology, Microbiota genetics
- Abstract
The microbiome is fundamental for understanding bacterial activities in sediments. However, only a limited number of studies have addressed the microbial diversity of Amazonian sediments. Here, we studied the microbiome of sediments from a 13,000-year BP core retrieved in a floodplain lake in Amazonia using metagenomics and biogeochemistry. Our aim was to evaluate the possible environmental influence over a river to a lake transition using a core sample. To this end, we sampled a core in the Airo Lake, a floodplain lake in the Negro River basin. The Negro River is the largest tributary of the Amazon River. The obtained core was divided into three strata: (i) surface, almost complete separation of the Airo Lake from the Negro River when the environment becomes more lentic with greater deposition of organic matter (black-colored sediment); (ii) transitional environment (reddish brown); and (iii) deep, environment with a tendency for greater past influence of the Negro River (brown color). The deepest sample possibly had the greatest influence of the Negro River as it represented the bottom of this river in the past, while the surface sample is the current Airo Lake bottom. In total, six metagenomes were obtained from the three different depth strata (total number of reads: 10.560.701; sequence length: 538 ± 24, mean ± standard deviation). The older (deeper) sediment strata contained a higher abundance of Burkholderia, Chitinophaga, Mucilaginibacter, and Geobacter, which represented ~ 25% of the metagenomic sequences. On the other hand, the more recent sediment strata had mainly Thermococcus, Termophilum, Sulfolobus, Archaeoglobus, and Methanosarcina (in total 11% of the metagenomic sequences). The sequence data were binned into metagenome-assembled genomes (MAGs). The majority of the obtained MAGs (n = 16) corresponded to unknown taxa, suggesting they may belong to new species. The older strata sediment microbiome was enriched with sulfur cycle genes, TCA cycle, YgfZ, and ATP-dependent proteolysis in bacteria. Meanwhile, serine-glyoxylate cycle, stress response genes, bacterial cell division, cell division-ribosomal stress protein cluster, and oxidative stress increased in the younger strata. Metal resistance and antimicrobial resistance genes were found across the entire core, including genes coding for fluoroquinolones, polymyxin, vancomycin, and multidrug resistance transporters. These findings depict the possible microbial diversity during the depositional past events and provided clues of the past microbial metabolism throughout time., (© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
- Published
- 2023
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11. Modeling water transport properties in carbon nanotubes: Interplay between force-field flexibility and geometrical parameters.
- Author
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Moreira LS, de Vargas DD, and Köhler MH
- Abstract
Modeling water and other liquids in computational simulations requires a large set of parameters. Many works have been devoted to finding new, improved water models, with almost all of them designed for bulk systems. Here, we use carbon nanotubes as a play model to investigate the effects of introducing flexibility in water force fields during molecular dynamics simulations of nanoconfined water. We explore six different models to show that viscosity, diffusion, and dipole orientation are vastly influenced by the flexibility and the family of force fields used. Particularly, we found the level of confinement (decreasing the nanotube's diameter) to increase discrepancies in the description of the dipole alignment. In smaller (10,10) nanotubes, the flexible version of the transferable intermolecular potential with three points (TIP3P/Fs) features a high directionality, while its rigid counterpart shows a more distributed dipole orientation. Both viscosity and diffusion are also extremely dependent on the force-field family, with the flexible version of the simple point charge (SPC/Fw) featuring the lower confidence interval.
- Published
- 2023
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12. Author Correction: Tropical forests as drivers of lake carbon burial.
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Amora-Nogueira L, Sanders CJ, Enrich-Prast A, Sanders LSM, Abuchacra RC, Moreira-Turcq PF, Cordeiro RC, Gauci V, Moreira LS, Machado-Silva F, Libonati R, Fonseca T, Francisco CN, and Marotta H
- Published
- 2023
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13. Factors influencing terrestriality in primates of the Americas and Madagascar.
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Eppley TM, Hoeks S, Chapman CA, Ganzhorn JU, Hall K, Owen MA, Adams DB, Allgas N, Amato KR, Andriamahaihavana M, Aristizabal JF, Baden AL, Balestri M, Barnett AA, Bicca-Marques JC, Bowler M, Boyle SA, Brown M, Caillaud D, Calegaro-Marques C, Campbell CJ, Campera M, Campos FA, Cardoso TS, Carretero-Pinzón X, Champion J, Chaves ÓM, Chen-Kraus C, Colquhoun IC, Dean B, Dubrueil C, Ellis KM, Erhart EM, Evans KJE, Fedigan LM, Felton AM, Ferreira RG, Fichtel C, Fonseca ML, Fontes IP, Fortes VB, Fumian I, Gibson D, Guzzo GB, Hartwell KS, Heymann EW, Hilário RR, Holmes SM, Irwin MT, Johnson SE, Kappeler PM, Kelley EA, King T, Knogge C, Koch F, Kowalewski MM, Lange LR, Lauterbur ME, Louis EE Jr, Lutz MC, Martínez J, Melin AD, de Melo FR, Mihaminekena TH, Mogilewsky MS, Moreira LS, Moura LA, Muhle CB, Nagy-Reis MB, Norconk MA, Notman H, O'Mara MT, Ostner J, Patel ER, Pavelka MSM, Pinacho-Guendulain B, Porter LM, Pozo-Montuy G, Raboy BE, Rahalinarivo V, Raharinoro NA, Rakotomalala Z, Ramos-Fernández G, Rasamisoa DC, Ratsimbazafy J, Ravaloharimanitra M, Razafindramanana J, Razanaparany TP, Righini N, Robson NM, Gonçalves JDR, Sanamo J, Santacruz N, Sato H, Sauther ML, Scarry CJ, Serio-Silva JC, Shanee S, Lins PGAS, Smith AC, Smith Aguilar SE, Souza-Alves JP, Stavis VK, Steffens KJE, Stone AI, Strier KB, Suarez SA, Talebi M, Tecot SR, Tujague MP, Valenta K, Van Belle S, Vasey N, Wallace RB, Welch G, Wright PC, Donati G, and Santini L
- Subjects
- Americas, Animals, Cercopithecidae, Haplorhini, Humans, Madagascar, Mammals, Trees, Biological Evolution, Primates
- Abstract
Among mammals, the order Primates is exceptional in having a high taxonomic richness in which the taxa are arboreal, semiterrestrial, or terrestrial. Although habitual terrestriality is pervasive among the apes and African and Asian monkeys (catarrhines), it is largely absent among monkeys of the Americas (platyrrhines), as well as galagos, lemurs, and lorises (strepsirrhines), which are mostly arboreal. Numerous ecological drivers and species-specific factors are suggested to set the conditions for an evolutionary shift from arboreality to terrestriality, and current environmental conditions may provide analogous scenarios to those transitional periods. Therefore, we investigated predominantly arboreal, diurnal primate genera from the Americas and Madagascar that lack fully terrestrial taxa, to determine whether ecological drivers (habitat canopy cover, predation risk, maximum temperature, precipitation, primate species richness, human population density, and distance to roads) or species-specific traits (body mass, group size, and degree of frugivory) associate with increased terrestriality. We collated 150,961 observation hours across 2,227 months from 47 species at 20 sites in Madagascar and 48 sites in the Americas. Multiple factors were associated with ground use in these otherwise arboreal species, including increased temperature, a decrease in canopy cover, a dietary shift away from frugivory, and larger group size. These factors mostly explain intraspecific differences in terrestriality. As humanity modifies habitats and causes climate change, our results suggest that species already inhabiting hot, sparsely canopied sites, and exhibiting more generalized diets, are more likely to shift toward greater ground use.
- Published
- 2022
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14. The Association of Bacterin and Recombinant Proteins Induces a Humoral Response in Sheep against Caseous Lymphadenitis.
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Moreira LS, Lopes NDR, Pereira VC, Andrade CLB, Torres AJL, Ribeiro MB, Freire SM, Santos RMD, D'ávila M, Nascimento RM, and Marchioro SB
- Abstract
In this study, we investigated the capacity of the recombinant proteins SpaC, NanH, SodC, and PLD of C. pseudotuberculosis to trigger protective humoral and cellular immune responses against experimentally induced C. pseudotuberculosis infection in sheep. The antigens were produced in a heterologous system and were purified by affinity chromatography. Nine sheep were randomly divided into three groups, which were immunized as follows: Group 1 (control)-a mix of adjuvants composed of the inactivated T1 strain of C. pseudotuberculosis and commercial Montanide™ISA 61 VG (T1M); Group 2-rSpaC, rSodC, rPLD, and T1M; Group 3-rNanH, rSodC, rPLD, and T1M. All groups were immunized twice (on days 0 and 30) and challenged on day 90 of the experiment. Humoral and cellular immune responses were evaluated by Enzyme-Linked Immunosorbent Assay (ELISA) to quantify the IgG antibodies and interferon-gamma (IFN-y). Both vaccine formulations with recombinant proteins (groups 2 and 3) could induce a significant humoral IgG immune response in sheep. The proteins rSodC, rPLD, and rNanH were more immunogenic, inducing significant levels of IgG antibodies after the first dose of the vaccine or after the challenge, maintaining constant levels until the end of the experiment. However, it was not possible to differentiate between the cellular responses induced by the vaccines. This lack of effectiveness points toward the need for further studies to improve the efficacy of this subunit-based vaccine approach.
- Published
- 2022
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15. Tropical forests as drivers of lake carbon burial.
- Author
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Amora-Nogueira L, Sanders CJ, Enrich-Prast A, Sanders LSM, Abuchacra RC, Moreira-Turcq PF, Cordeiro RC, Gauci V, Moreira LS, Machado-Silva F, Libonati R, Fonseca T, Francisco CN, and Marotta H
- Subjects
- Carbon Sequestration, Ecosystem, Forests, Geologic Sediments, Tropical Climate, Carbon, Lakes
- Abstract
A significant proportion of carbon (C) captured by terrestrial primary production is buried in lacustrine ecosystems, which have been substantially affected by anthropogenic activities globally. However, there is a scarcity of sedimentary organic carbon (OC) accumulation information for lakes surrounded by highly productive rainforests at warm tropical latitudes, or in response to land cover and climate change. Here, we combine new data from intensive campaigns spanning 13 lakes across remote Amazonian regions with a broad literature compilation, to produce the first spatially-weighted global analysis of recent OC burial in lakes (over ~50-100-years) that integrates both biome type and forest cover. We find that humid tropical forest lake sediments are a disproportionately important global OC sink of ~80 Tg C yr
-1 with implications for climate change. Further, we demonstrate that temperature and forest conservation are key factors in maintaining massive organic carbon pools in tropical lacustrine sediments., (© 2022. The Author(s).)- Published
- 2022
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16. Alpha-tocopherol-loaded polycaprolactone nanoparticles improve the inflammation and systemic oxidative stress of arthritic rats.
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Moreira LS, Chagas AC, Ames-Sibin AP, Pateis VO, Gonçalves OH, Silva-Comar FMS, Hernandes L, Sá-Nakanishi AB, Bracht L, Bersani-Amado CA, Bracht A, and Comar JF
- Abstract
Background and Aim: The present study investigated the effects of orally administered α-tocopherol-loaded polycaprolactone nanoparticles on the articular inflammation and systemic oxidative status of middle-aged Holtzman rats with Freund's adjuvant-induced polyarthritis, a model for rheumatoid arthritis. Intraperitoneally administered free α-tocopherol provided the reference for comparison., Experimental Procedure: Two protocols of treatment were followed: intraperitoneal administration of free α-tocopherol (100 mg/kg i.p.) or oral administration of free and nanoencapsulated α-tocopherol (100 mg/kg p.o.). Animals were treated during 18 days after arthritis induction., Results: Free (i.p.) and encapsulated α-tocopherol decreased the hind paws edema, the leukocytes infiltration into femorotibial joints and the mRNA expression of pro-inflammatory cytokines in the tibial anterior muscle of arthritic rats, but the encapsulated compound was more effective. Free (i.p.) and encapsulated α-tocopherol decreased the high levels of reactive oxygen species in the brain and liver, but only the encapsulated compound decreased the levels of protein carbonyl groups in these organs. Both free (i.p.) and encapsulated α-tocopherol increased the α-tocopherol levels and the ratio of reduced to oxidized glutathione in these organs., Conclusion: Both intraperitoneally administered free α-tocopherol and orally administered encapsulated α-tocopherol effectively improved inflammation and systemic oxidative stress in middle-aged arthritic rats. However, the encapsulated form should be preferred because the oral administration route does not be linked to the evident discomfort that is caused in general by injectable medicaments. Consequently, α-tocopherol-loaded polycaprolactone nanoparticles may be a promising adjuvant to the most current approaches aiming at rheumatoid arthritis therapy., Competing Interests: There are no conflicts of interest to declare., (© 2021 Center for Food and Biomolecules, National Taiwan University. Production and hosting by Elsevier Taiwan LLC.)
- Published
- 2021
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17. Low dose of quercetin-loaded pectin/casein microparticles reduces the oxidative stress in arthritic rats.
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Souza KS, Moreira LS, Silva BT, Oliveira BPM, Carvalho AS, Silva PS, Verri WA Jr, Sá-Nakanishi AB, Bracht L, Zanoni JN, Gonçalves OH, Bracht A, and Comar JF
- Subjects
- Alanine Transaminase blood, Animals, Antioxidants pharmacology, Arthritis, Experimental blood, Brain drug effects, Brain pathology, Calorimetry, Differential Scanning, Cell Respiration drug effects, Edema pathology, Liver drug effects, Liver pathology, Male, Mitochondria, Liver drug effects, Mitochondria, Liver metabolism, Oxidoreductases metabolism, Rats, Reactive Oxygen Species metabolism, Spectroscopy, Fourier Transform Infrared, X-Ray Diffraction, Arthritis, Experimental pathology, Caseins chemistry, Microspheres, Oxidative Stress drug effects, Pectins chemistry, Quercetin pharmacology
- Abstract
Aims: Quercetin has been investigated as an agent to treat rheumatoid arthritis. At high doses it improves inflammation and the antioxidant status of arthritic rats, but it also exerts mitochondriotoxic and pro-oxidant activities. Beneficial effects of quercetin have not been found at low doses because of its chemical instability and low bioavailability. In the hope of overcoming these problems this study investigated the effects of long-term administration of quercetin-loaded pectin/casein microparticles on the oxidative status of liver and brain of rats with adjuvant-induced arthritis., Main Methods: Particle morphology was viewed with transmission electron microscopy and the encapsulation efficiency was measured indirectly by X-ray diffraction. Quercetin microcapsules (10 mg/Kg) were orally administered to rats during 60 days. Inflammation indicators and oxidative stress markers were measured in addition to the respiratory activity and ROS production in isolated mitochondria., Key Findings: Quercetin was efficiently encapsulated inside the polymeric matrix, forming a solid amorphous solution. The administration of quercetin microparticles to arthritic rats almost normalized protein carbonylation, lipid peroxidation, the levels of reactive oxygen species as well as the reduced glutathione content in both liver and brain. The paw edema in arthritic rats was not responsive, but the plasmatic activity of ALT and the mitochondrial respiration were not affected by quercetin, indicating absence of mitochondriotoxic or hepatotoxic actions., Significance: Quercetin-loaded pectin/casein microcapsules orally administered at a low dose improve oxidative stress of arthritic rats without a strong anti-inflammatory activity. This supports the long-term use of quercetin as an antioxidant agent to treat rheumatoid arthritis., (Copyright © 2021 Elsevier Inc. All rights reserved.)
- Published
- 2021
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18. Self-organizing map applied to the choice of internal standards for the determination of Cd, Pb, Sn, and platinum group elements by inductively coupled plasma mass spectrometry.
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Moreira LS, Costa FS, Machado RC, Nogueira ARA, Gonzalez MH, da Silva EGP, and Amaral CDB
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- Animals, Mass Spectrometry, Platinum, Spectrum Analysis, Cadmium, Lead
- Abstract
The behaviors of internal standards, according to different flow rates of the cell collision gas (He), were studied for the determination of Cd, Pb, Pd, Pt, Rh, and Sn in samples of fish and mollusks by inductively coupled plasma mass spectrometry (ICP-MS). The elements Bi, Ge, In, Sc, and Y were selected as internal standards, considering their masses and first ionization energies. Addition and recovery experiments were carried out at three concentration levels to evaluate the accuracy of the method applied for the analysis of two samples with different matrices. The results were evaluated using a self-organizing map (SOM). The best analyte/IS pairs were as follows:
114 Cd+ /74 Ge+ ,195 Pt+ /74 Ge+ , and208 Pb+ /74 Ge+ . For103 Rh+ ,106 Pd+ , and120 Sn+ , greater accuracy was achieved without use of an internal standard. Helium gas (2.8 mL min-1 ) was used in the collision cell for the analytes, except for Sn, and recoveries ranged from 98 to 101% under optimal conditions. The use of SOM as an exploratory analysis tool was an effective approach for selection of the most appropriate internal standards., (Copyright © 2021 Elsevier B.V. All rights reserved.)- Published
- 2021
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19. Environmental and anthropic variabilities at Guanabara Bay (Brazil): A comparative perspective of metal depositions in different time scales during the last 5,500 yrs.
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Cordeiro RC, Monteiro FF, Santelli RE, Moreira LS, Figueiredo AG, Bidone ED, Pereira RS, Anjos LC, and Meniconi MFG
- Subjects
- Bays, Brazil, Environmental Monitoring, Geologic Sediments, Humans, Metals, Heavy analysis, Water Pollutants, Chemical analysis
- Abstract
The determination of age-dated metal sediment accumulation rates in a representative South American bay is able to portray the dimension of human impacts during the colonial occupation and industrial periods. Many studies have assessed metal distribution and chronology at Guanabara Bay, in Brazil. However, understanding natural variabilities associated to paleoclimatic changes and comparing these natural variabilities to anthropogenic processes are not well established to date. Accurate geochronological control integrating ages determined by
210 Pb and14 C chronologies through an exponential spline fit model allowed for a precise definition of changes associated to the holocene marine transgression, as well as the colonial period, leading to intense land use changes, and the industrial period. The reference values of the system were defined based on their concentrations and the accumulation rates of ecotoxicologically important metals. Al, Ba, Fe, Cd, Cu, Cr, Li, Ni, Mn, Pb, Si, Ti, V, and Zn distributions were determined in a Guanabara Bay core (BG-28) by the EPA 3051 method. Elemental distribution profile assessment allowed for the identification of variabilities associated to weathering processes, predominantly of lithogenic origin, mainly for Al, Ba, Fe, Li, Si, and V. Weathering processes occurred simultaneously to land use changes in the drainage basin since the colonial period, at 400 cal yr BP, and during the industrial period, mainly after the 1960s, denoted by Cd, Cr, Cu, Mn, Pb and Zn increases. The highest average metal enrichment values metals associated to industrial processes reached 5.95, with 119.1-fold higher accumulation rates than the background accumulation values observed between 4200 and 500 cal yr BP., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. I ask that the manuscript not be sent for review to Researchers of my institution (Universidade Federal Fluminense) due to professional proximity., (Copyright © 2020 Elsevier Ltd. All rights reserved.)- Published
- 2021
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20. New bacterial and archaeal lineages discovered in organic rich sediments of a large tropical Bay.
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Tschoeke DA, Coutinho FH, Leomil L, Cavalcanti G, Silva BS, Garcia GD, Dos Anjos LC, Nascimento LB, Moreira LS, Otsuki K, Cordeiro RC, Rezende CE, Thompson FL, and Thompson CC
- Subjects
- Archaea classification, Archaea genetics, Bacteria classification, Bacteria genetics, Brazil, Metagenomics, Phylogeny, Archaea isolation & purification, Bacteria isolation & purification, Bays microbiology, Geologic Sediments microbiology, Metagenome, Microbiota
- Abstract
The nutrient and oxygen gradient present in marine sediments promotes high levels of microbial diversity. We applied metagenomics and biogeochemical tools to analyze microbial communities in different sediment depths (0-4 m below sea floor, mbsf) from Guanabara Bay, Brazil, a brackish tropical ecosystem with a history of massive anthropogenic impacts, and a largely unknown sediment microbial diversity. Methanogens (e.g. Methanosarcinales, Methanomicrobiales) were more abundant at 1 mbsf, while sulphate-reducing microbes (Desulfurococcales, Thermoprotales, and Sulfolobales) were more abundant at deeper layers (4 mbsf; corresponding to 3 K Radiocarbon years before present, Holocene Epoch). Taxonomic analyzes and functional gene identification associated with anaerobic methane oxidation (e.g. monomethylamine methyltransferase (mtmB), trimethylamine methyltransferase (mttB) and CO dehydrogenase/acetyl-CoA synthase delta subunit) and sulfate reduction indicated the dominance of Campylobacteria (Sulfurimonas) at deeper sediment layers. Gene sequences related to assimilation of inorganic sulfur increased with depth, while organic sulfur related sequences decrease, accompanying the clear reduction in the concentration of sulfur, organic carbon and chla torwards deeper layers. Analyzes of metagenome assembled genomes also led to the discovery of a novel order within the phylum Acidobacteriota, named Guanabacteria. This novel order had several in silico phenotyping features that differentiate it from closely related phylogenetic neighbors (e.g. Acidobacteria, Aminicenantes, and Thermoanaerobaculum), including several genes (carbon monoxide dehydrogenase, CO dehydrogenase/CO-methylating acetyl-CoA synthase complex subunit beta, heterodisulfide reductase, sulfite exporter TauE/SafE family protein, sulfurtransferase) that relevant for the S and C cycles. Furthermore, the recovered Bathyarchaeota genome SS9 illustrates the methanogenic potential in deeper sediment layer., (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Published
- 2020
- Full Text
- View/download PDF
21. NEOTROPICAL ALIEN MAMMALS: a data set of occurrence and abundance of alien mammals in the Neotropics.
- Author
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Rosa CAD, Ribeiro BR, Bejarano V, Puertas FH, Bocchiglieri A, Barbosa ALDS, Chiarello AG, Paglia AP, Pereira AA, Moreira AFS, Souza AC, Pellegrin A, Gatica A, Medeiro AZ, Pereira AD, Braz AG, Yanosky A, Valenzuela AEJ, Bertassoni A, Prado ADSVD, Nava AFD, Rocha A, Bovo AAA, Bager A, Cravino A, Pires ADS, Martensen AC, Filippini A, Percequillo AR, Vogliotti A, Antunes AZ, Leite de Oliveira AC, da Silva de Oliveira AJ, Devlin A, de Paula A, Ferreira AS, García-Olaechea A, Subalusky A, Sánchez A, de Aquino ACMM, Srbek-Araujo AC, Paldês Gonçales A, Araújo ACL, Gozzi AC, Ochoa AC, Mendes de Oliveira AC, Lacerda ACR, Francisco AK, Paschoal AMO, Gomes APN, Potrich AP, Olímpio APM, Rojas A, Meiga AYY, Jácomo ATA, Calaça AM, Feijó A, Pagoto A, Borja Miranda A, Chein Alonso A, Barreto-Lima AF, Lanna A, Luza AL, Camilo AR, Tavares A, Nunes AV, Kindel A, de Miguel A, Gatti A, Nobre AB, Campêlo ADC, Albuquerque ACF, de la Torre A, Mangione A, Mendes Pontes AR, Fernandes AS, Felicio ALA, Ferreguetti AC, Marcili A, Piratelli AJ, Nascimento AGSD, Banhos Dos Santos Á, Rosa BF, Cezila BA, de Thoisy B, Ingberman B, Köhler B, Morais BC, Gómez-Valencia B, Bertagni de Camargo B, Bezerra BM, Tamasauskas B, Parahyba Campos BAT, Kubiak BB, Saranholi BH, Nakagawa BK, Leles BP, Lim BK, Pereira Mendes C, Islas CA, Aoki C, Cantagallo Devids C, Figueiredo C, Abreu CMG, Silva Oliveira CR, Cassano CR, Lugarini C, Caputo C, Gestich CC, Tedesco CD, Vera Y Conde CF, Hegel CGZ, Kasper CB, De Angelo C, Grelle CEV, Fragoso CE, Esbérard CEL, Rocha CFD, Verona CE, Salvador CH, Vieira CL, Abrahão CR, Brocardo CR, Fieker CZ, Braga C, Sánchez Lalinde C, Bueno C, Ikuta CY, Luna CLB, Cestari C, Del Vechio Koike C, Knogge C, Anderson CB, Hurtado CM, Ferreira Antunes de Oliveira C, Tellaeche C, Cesário CS, Costa CG, Kanda CZ, Costa SA, Seixas CS, Trinca CT, López-Fuerte CF, da Cunha CJ, Doutel Ribas C, Santos CC, Buscariol D, Carreira D, Nascimento DCD, Carvalho DR, Ferraz DDS, Galiano D, Homem DH, Jesús-Espinosa D, Bôlla DAS, Moreno DJ, Moreira DO, Ramos DL, de Amorim DA, Barros-Battesti DM, Lopez DE, Tavares DC, Post DM, Couto DR, Patrocínio DN, Carvalho DLKP, Silva DA, Córdoba D, Queirolo D, Varela D, de Oliveira DAG, Casanova DC, Dias DM, Machado da Silva D, Barbier E, Rivadeneira EF, Alexandrino E, Carrano E, Santos EM, Venticinque EM, Hernández-Pérez E, Casazza EDF, Anderson EP, Fraga EDC, de Lima EF, D'Bastiani E, Vieira EM, Guijosa-Guadarrama E, González EM, Maggiorini EV, Aguiar EFS, Martínez-Nambo ED, Castro ÉP, de la Peña-Cuéllar E, Pedó E, Melo FCSA, Rocha FL, Fonseca FL, Girardi F, Melo FR, Roque FO, Keesen Ferreira F, Peters FB, Moreli Fantacini F, Pedrosa F, Pessoa da Silva F, Vélez-García F, Abra FD, de Azevedo FC, Guedes da Silva F, Neri FM, Teixeira FZ, Fernandez FADS, Carvalho F, Passos FC, Jacinavicius FC, Ferreira F, Pinho FF, Gonçalves F, Ibanez Martins F, Lima F, Contreras-Moreno FM, Ribeiro FS, Tortato F, Patel FM, Caruso F, Tirelli FP, Rodrigues FHG, Ubaid FK, Palmeira FBL, Grotta Neto F, Gabriel FH, de Souza FL, Costa FEDVD, de Aguiar GL, Lemos FG, Magezi GS, Panigai GFVD, Hofmann GS, Heliodoro G, Rosa Graviola G, Beca G, Andrade GR, Jiménez Romero G, Duarte GT, Melo GL, Dierings GL, Sabino-Santos G Jr, de Oliveira GL, Santana GG, Ciocheti G, Zanirato GL, Alves GB, Batista GO, Behling GM, Ferreira GB, da Rocha GC, Lessa G, Mourão G, Maras GA, Toledo GADC, Gonsioroski G, Canale GR, Schuchmann KL, Sebastião H, Alves do Prado H, Bergallo HG, Secco HKC, Roig HL, Rajão H, Carlos HSA, Duarte HOB, Ermenegildo H, Pena HFJ, Entringer Júnior H, Paulino Neto HF, Lemos HM, Del Castillo H, Fernandes-Ferreira H, Coitiño Banquero HI, Roesler I, Ribeiro IK, Coelho IP, Lima IMS, Bechara IM, Lermen IS, Mella Méndez I, Schuck G, Esperandio IB, Silva IO, Mourthe I, Oliveira I, Bernardi IP, Miller JR, Marinho-Filho J, Zocche JJ, Russell JC, Seibert JB, Hinojosa J, Vitule JRS, Thompson JJ, Silva JCR, Gouvea JA, Santos JP, Falcão JCF, Castro-Prieto J, Ferreira JP, Pincheira-Ulbrich J, Nodari JZ, Zecchini Gebin JC, Giovanelli JGR, Miranda JMD, Souza-Alves JP, Marins JRGA, Costa JF, Sponchiado J, de Souza JL, Gallo JA, Cherem JJ, Cordeiro JLP, Duarte JMB, Dantas JO, de Matos JR, Pires JSR, Martínez Lanfranco JA, de la Cruz Godoy JC, Rudolf JC, Parrish JFR, Tellarini JF, Peña-Mondragón JL, Arrabal JP, Reppucci J, Ruiz-Esparza J, Beduschi J, Oshima JEF, Ribeiro JF, Almeida Rocha JM, Ferreira Neto JS, Silveira Dos Santos J, Pereira-Ribeiro J, Zanoni JB, Bogoni JA, Ferreira JR, Bicca-Marques JC, Chacón Pacheco JJ, Scarascia PO, Guidoni-Martins KG, Burs K, Ferraz KMPMB, Pisciotta KR, Silva KVKA, Juarez KM, de la Cruz-Félix K, de Morais KDR, Candelária LP, Fornitano L, Bailey LL, Gonçalves LO, Fasola L, Nova León LJ, de Andrade LR, Marques LO, Macedo L, Moreira LS, Silveira L, Oliveira LC, da Silva LH, Jerusalinsky L, La Serra L, Marques Costa L, Sartorello LR, Munhoes LP, Oliveira-Silva LRB, de Pina LF, Bonjorne L, Rampim LE, Sales LP, Gonçalves da Silva L, Quintilham LLT, Perillo LN, Rodríguez-Planes LI, Martín L, Araújo LS, Tiepolo LM, Zago Silva L, García Loaiza LM, Querido LCA, da Silva LF, La Sala LF, Bopp LT, Hufnagel L, Oliveira LFB, Oliveira-Santos LGR, Lyra LH, Guimarães LN, Jimenez Segura LF, de Sousa LC, Möcklinghoff L, Guichón ML, de la Maza J, Barrios-Garcia MN, Talamoni SA, Severo MM, Martins MZA, Oliveira MA, Figuerêdo Duarte Moraes M, Lima MGM, Soares Pinheiro M, Pônzio MDC, Guerreiro M, Cervini M, da Silva M, Oliveira MJR, Magioli M, Passamani M, Silva de Almeida M, Amaku M, Leite de Oliveira M, Tortato MA, Melo MA, Coutinho ME, Dantas Santos MP, Vieira MV, Andrade MA, Barros MC, Rosario MCFD, Domit MDADS, Fernandes MEA, Iezzi ME, do Nascimento MHS, Andrade-Núñez MJ, Lorini ML, Morini MSC, Nagy-Reis MB, Landis MB, Vale MM, Xavier MS, Kaizer MC, Baptiste MP, Bergel MM, Borgnia M, Barros MAS, Lima da Silva M, Favarini MO, Sales Munerato M, Zaluar MT, Winter M, Xavier da Silva M, Zanin M, Marques MI, Haberfeld MB, Di Bitetti MS, Galliez M, Alvarez MR, Malerba M, Rivero M, Melo Dias M, de Oliveira MY, Dos Reis MG, Corrêa MRJ, Graipel ME, Godoi MN, Núñez-Regueiro MM, Moura MO, Orsi ML, Galvão da Silva MA, Sanvicente Lopez M, Benedetti MA, Beltrão MG, Camino M, Faria MB, Miretzki M, Luiz MR, Perine M, Monteiro MCM, Alves-Eigenheer M, Perilli MLL, da Silva MA, Marini MÂ, Silva Pereira M, de Freitas Junior MC, Cossa N, Denkiewicz NM, Tôrres NM, Olifiers N, de Albuquerque NM, Canassa NF, Detogne N, Gurgel Filho N, Seoane NF, da Rosa Oliveira N, Megale N, Pasqualotto N, Cáceres NC, Peroni N, Zanella N, Pays O, Arimoro OAS, Acevedo-Charry O, de Almeida Curi NH, Pinha PRS, Perovic P, Gonçalves PR, Santos PM, Brennand PGG, Kerches Rogeri P, Rosas Ribeiro P, da Rocha PA, de Lázari PR, Pedreira PA, Pinheiro PF, Lira PK, Ferreira PM, Martin PS, Antas PTZ, Marinho PH, Ruffino PHP, Camargo PHSA, Landgref Filho P, Mangini PR, Farias P, Cordeiro-Estrela P, de Faria Peres PH, Galetti PM Jr, Ramírez-Bautista P, Maués PCRA, Renaud PC, Sartorello R, Barros PA, Lombardi PM, Bessa R, Arroyo-Gerala P, de Souza RCC, Zenni RD, Flores Peredo R, Hoogesteijn R, Loyola R, Alves RSC, Rodarte RRP, Silva RL, de Oliveira R, Beltrão-Mendes R, Alencar RM, da Silva RC, Pedroso R, Sampaio RF, Ribeiro RLA, Pardini R, Twardowsky Ramalho Bonikowski R, Pagotto RV, Dias RA, Bassini-Silva R, Corassa Arrais R, Sampaio R, de Cassia Bianchi R, Paolino RM, Fusco-Costa R, Trovati RG, Espíndola Hack RO, Mauro RA, Nobre RA, Gessulli RD, León Pérez R, Massara RL, Fróes da Silva RM, de Paula RC, da Cunha RGT, Costa RT, Marques RV, Morato RG, Bovendorp RS, Dornas RADP, Andrade RS, Siciliano S, Guaragni SA, Rolim SG, Astete S, Cavalcanti S, Hartz SM, Carvalho S, Cortez S, Silvestre de Sousa SM, Ballari SA, Ramos Lima S, Cirignoli S, García-R S, Bazilio S, Solari Torres S, Back Franco S, Martins SR, de Bustos S, Age SG, Ferrari SF, Francisco TM, Micheletti T, Godim TMDS, Luiz TG, Ochotorena de Freitas TR, Rodrigues TF, Piovezan U, Barcos UC, Onofrio VC, Martin-Albarracin VL, Towns V, Araújo VC, Kanaan V, Daga VS, Boere V, de Araujo VPG, Benitez VV, Leandro-Silva V, Geraldi VC, Alberici V, Bastazini VAG, Gasparotto VPO, Orsini VS, da Silva VS, Rojas Bonzi V, Pereira VJA, Layme VMG, Duarte da Silva VH, Tomas WM, Moreira TA, Martins WP, de Moraes Pires WM, Hannibal W, Dáttilo W, Mottin V, Endo W, Bercê W, Carvalho WD, Magnusson W, Akkawi P, Di Blanco Y, Amaral PR, Ramos YGC, Rodríguez-Calderón YG, Mendes YR, Ribeiro YGG, Campos Z, Galetti M, and Ribeiro MC
- Subjects
- Animals, Argentina, Biodiversity, Cattle, Chile, Dogs, Florida, Mexico, Introduced Species, Mammals
- Abstract
Biological invasion is one of the main threats to native biodiversity. For a species to become invasive, it must be voluntarily or involuntarily introduced by humans into a nonnative habitat. Mammals were among first taxa to be introduced worldwide for game, meat, and labor, yet the number of species introduced in the Neotropics remains unknown. In this data set, we make available occurrence and abundance data on mammal species that (1) transposed a geographical barrier and (2) were voluntarily or involuntarily introduced by humans into the Neotropics. Our data set is composed of 73,738 historical and current georeferenced records on alien mammal species of which around 96% correspond to occurrence data on 77 species belonging to eight orders and 26 families. Data cover 26 continental countries in the Neotropics, ranging from Mexico and its frontier regions (southern Florida and coastal-central Florida in the southeast United States) to Argentina, Paraguay, Chile, and Uruguay, and the 13 countries of Caribbean islands. Our data set also includes neotropical species (e.g., Callithrix sp., Myocastor coypus, Nasua nasua) considered alien in particular areas of Neotropics. The most numerous species in terms of records are from Bos sp. (n = 37,782), Sus scrofa (n = 6,730), and Canis familiaris (n = 10,084); 17 species were represented by only one record (e.g., Syncerus caffer, Cervus timorensis, Cervus unicolor, Canis latrans). Primates have the highest number of species in the data set (n = 20 species), partly because of uncertainties regarding taxonomic identification of the genera Callithrix, which includes the species Callithrix aurita, Callithrix flaviceps, Callithrix geoffroyi, Callithrix jacchus, Callithrix kuhlii, Callithrix penicillata, and their hybrids. This unique data set will be a valuable source of information on invasion risk assessments, biodiversity redistribution and conservation-related research. There are no copyright restrictions. Please cite this data paper when using the data in publications. We also request that researchers and teachers inform us on how they are using the data., (© 2020 The Authors. Ecology © 2020 The Ecological Society of America.)
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- 2020
- Full Text
- View/download PDF
22. NEOTROPICAL CARNIVORES: a data set on carnivore distribution in the Neotropics.
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Nagy-Reis M, Oshima JEF, Kanda CZ, Palmeira FBL, de Melo FR, Morato RG, Bonjorne L, Magioli M, Leuchtenberger C, Rohe F, Lemos FG, Martello F, Alves-Eigenheer M, da Silva RA, Silveira Dos Santos J, Priante CF, Bernardo R, Rogeri P, Assis JC, Gaspar LP, Tonetti VR, Trinca CT, Ribeiro AS, Bocchiglieri A, Hass A, Canteri A, Chiarello AG, Paglia AP, Pereira AA, de Souza AC, Gatica A, Medeiro AZ, Eriksson A, Costa AN, González-Gallina A, Yanosky AA, Jesus de la Cruz A, Bertassoni A, Bager A, Bovo AAA, Cravino Mol A, Bezerra AMR, Percequillo A, Vogliotti A, Costa Lopes AM, Keuroghlian A, Zúñiga Hartley AC, Devlin AL, de Paula A, García-Olaechea A, Sánchez A, Aquino ACMM, Srbek-Araujo AC, Ochoa AC, Tomazzoni AC, Lacerda ACR, Bacellar AEF, Campelo AKN, Herrera Victoria AM, Paschoal AMO, Potrich AP, Gomes APN, Olímpio APM, Cunha Costa AR, Jácomo ATA, Calaça AM, Jesus AS, de Barros Barban A, Feijó A, Pagoto A, Rolim AC, Hermann AP, Souza ASMCE, Chein Alonso A, Monteiro A, Mendonça AF, Luza AL, Moura ALB, da Silva ALF, Lanna AM, Antunes AP, Nunes AV, Dechner A, Carvalho AS, Novaro AJ, Scabin AB, Gatti A, Nobre AB, Montanarin A, Deffaci ÂC, de Albuquerque ACF, Mangione AM, Pinto AMS, Mendes Pontes AR, Bertoldi AT, Calouro AM, Fernandes A, Ferreira AN, Ferreguetti AC, Rosa ALM, Banhos A, Francisco BDSS, Cezila BA, Beisiegel BM, de Thoisy B, Ingberman B, Neves BDS, Pereira-Silva B, Bertagni de Camargo B, Andrade BDS, Santos BS, Leles B, Torres Parahyba Campos BA, Kubiak BB, França BRA, Saranholi BH, Pereira Mendes C, Cantagallo Devids C, Pianca C, Rodrigues C, Islas CA, de Lima CA, de Lima CR, Gestich CC, Tedesco CD, De Angelo C, Fonseca C, Hass C, Peres CA, Kasper CB, Durigan CC, Fragoso CE, Verona CE, Rocha CFD, Salvador CH, Vieira CL, Ruiz CEB, Cheida CC, Sartor CC, Espinosa CDC, Fieker CZ, Braga C, Sánchez-Lalinde C, Machado CIC, Cronemberger C, Luna CL, Del Vechio C, Bernardo CSS, Hurtado CM, Lopes CM, da Rosa CA, Cinta CC, Costa CG, Zárate-Castañeda CP, Novaes CL, Jenkins CN, Seixas CS, Martin C, Zaniratto CP, López-Fuerte CF, da Cunha CJ, De-Carvalho CB, Chávez C, Santos CC, Polli DJ, Buscariol D, Carreira DC, Galiano D, Thornton D, Ferraz DDS, Lamattina D, Moreno DJ, Moreira DO, Farias DA, Barros-Battesti DM, Tavares DC, Costa Braga D, Gaspar DA, Friedeberg D, Astúa D, Silva DA, Viana DC, Lizcano DJ, Varela DM, Loretto D, Gräbin DM, Eaton DP, Machado da Silva D, Dias DM, Camara EMVC, Barbier E, Chávez-González E, Rocha EC, Lima ES, Carrano E, Eizirik E, Nakano-Oliveira E, Rigacci ED, Santos EM, Venticinque EM, Alexandrino ER, Abreu Ribeiro E, Setz E, Rocha ECLD, Carvalho EAR Jr, Rechenberg E, Fraga EDC, Mendonça EN, D'Bastiani E, Isasi-Catalá E, Guijosa-Guadarrama E, Ramalho EE, González E, Hasui É, Saito EN, Fischer E, Aguiar EF, Rocha ES, Martínez Nambo ED, de la Peña-Cuéllar E, Castro ÉP, de Freitas EB, Pedó E, Rocha FL, Girardi F, Pereira FA, Soares FAM, Roque FO, Díaz-Santos FG, Patiu FM, do Nascimento FO, Keesen Ferreira F, Diaz-Santos F, Moreli Fantacini F, Pedrosa F, Pessoa da Silva F, Velez-Garcia F, Gomes FBR, Guedes da Silva F, Michalski F, de Azevedo FC, de Barros FC, Santos FDS, Abra FD, Ramalho FDP, Hatano FM, Anaguano-Yancha F, Gonçalves F, Pedroni F, Passos FC, Jacinavicius FC, Bonfim FCG, Puertas FH, Contreras-Moreno FM, Tortato FR, Santos FM, Chaves FG, Tirelli FP, Vilas Boas FE, Rodrigues FHG, Ubaid FK, Grotta-Neto F, Palomares F, Souza FL, Costa FE, França FGR, Ramírez Pinto F, Aguiar GL, Hofmann GS, Heliodoro G, Duarte GT, Ribeiro de Andrade G, Beca G, Zapata-Ríos G, Giné GAF, Powell GVN, Wilson Fernandes G, Forero-Medina G, Melo GL, Santana GG, Ciocheti G, Alves GB, Souto GHBO, Villarroel GJ, Porfirio GEO, Batista GO, Behling GM, Ayala Crespo GM, Mourão GM, Rezende GZ, Toledo GADC, Herrera HM, Alves Prado H, Bergallo HG, Secco H, Rajão H, Roig HL, Concone HVB, Duarte H, Ermenegildo H, Ferreira Paulino Neto H, Quigley H, Lemos HM, Cabral H, Fernandes-Ferreira H, Del Castillo HF, Ribeiro IK, Coelho IP, Franceschi IC, Melo I, Oliveira-Bevan I, Mourthe I, Bernardi I, de la Torre JA, Marinho-Filho J, Martinez J, Palacios Perez JX, Pérez-Torres J, Bubadué J, Silveira JR, Seibert JB, Oliveira JF, Assis JR, De la Maza J, Hinojosa J, Metzger JP, Thompson JJ, Svenning JC, Gouvea JA, Souza JRD, Pincheira-Ulbrich J, Nodari JZ, Miranda J, Zecchini Gebin JC, Giovanelli JGR, Rossi Junior JL, Pandini Favoretti JP, Villani JP, Just JPG, Souza-Alves JP, Costa JF, Rocha J, Polisar J, Sponchiado J, Cherem JJ, Marinho JR, Ziegler J, Cordeiro J, de Sousa E Silva Júnior J, Rodriguez-Pulido JA, Chaves Dos Santos JC, Dos Reis Júnior JC, Mantovani JE, Moreira Ramírez JF, Sarasola JH, Cartes JL, Duarte JMB, Longo JM, Dantas JO, Venancio JO, de Matos JR, Pires JSR, Hawes JE, Santos JG, Ruiz-Esparza J, Martínez Lanfranco JA, Rudolf JC, Charre-Medellin JF, Zanón-Martínez JI, Peña-Mondragón JL, Campos Krauer JM, Arrabal JP, Beduschi J, Ilha J, Mata JC, Bonanomi J, Jordao J, de Almeida-Rocha JM, Pereira-Ribeiro J, Zanoni JB, Bogoni JA, Chacón Pacheco JJ, Contreras Palma KM, Strier KB, Rodriguez Castro KG, Didier K, Schuchmann KL, Chávez-Congrains K, Burs K, Ferraz KMPMB, Juarez KM, Flesher K, Morais KDR, Lautenschlager L, Grossel LA, Dahmer LC, de Almeida LR, Fornitano L, Barbosa LNB, Bailey LL, Barreto LN, Villalba LM, Magalhães LM, Cullen L Jr, Marques L, Marques Costa L, Silveira L, Moreira LS, Sartorello L, Oliveira LC, Gomes LP, Aguiar LDS, da Silva LH, Mendonça LS, Valenzuela LA, Benavalli L, Dias LCS, Munhoes LP, Catenacci L, Rampim LE, de Paula LM, Nascimento LA, Gonçalves da Silva L, Quintilham L, Ramis Segura L, Perillo LN, Rezende LR, Martínez Retta L, Rojas LNS, Guimarães LN, Araújo L, Zago da Silva L, Querido LCA, Verdade LM, Perera-Romero LE, Carvalho-Leite LJ, Hufnagel L, Rezende Bernardo LR, Oliveira LF, Oliveira Santos LGR, Lyra LH, Borges LHM, Severo MM, Benchimol M, Quatrocchi MG, Martins MZA, Rodrigues M, Penteado MJF, Figuerêdo Duarte Moraes M, Oliveira MA, Lima MGM, Pônzio MDC, Cervini M, da Silva M, Passamani M, Villegas MA, Dos Santos Junior MA, Yamane MH, Jardim MMA, Leite de Oliveira M, Silveira M, Tortato MA, Figueiredo MSL, Vieira MV, Sekiama ML, Andrade da Silva MA, Nuñez MB, Siviero MB, Carrizo MC, Barros MC, Barros MAS, do Rosário MCF, Peñuela Mora MC, Fleytas Jover MDC, Morandi MEF, Huerta ME, Fernandes MEA, Viscarra Siñani ME, Iezzi ME, Ramos Pereira MJ, Gomez Vinassa ML, Lorini ML, Jorge MLSP, Morini MS, Guenther M, Landis MB, Vale MM, Xavier MS, Tavares MS, Kaizer M, Velilla M, Bergel MM, Hartmann MT, Lima da Silva M, Rivero M, Salles Munerato M, Xavier da Silva M, Zanin M, Marques MI, Haberfeld M, Di Bitetti MS, Bowler M, Galliez M, Ortiz-Moreno ML, Buschiazzo M, Montes MA, Alvarez MR, Melo-Dias M, Reis MG, Corrêa MRJ, Tobler MW, Gompper ME, Nunez-Regueiro M, Brandão Vecchi M, Graipel ME, Godoi MN, Moura MO, Konzen MQ, Pardo MV, Beltrão MG, Mongelli M, Almeida MO, Gilmore MP, Schutte M, Faria MB, Luiz MR, de Paula M, Hidalgo-Mihart MG, Perilli MLL, Freitas-Junior MC, da Silva MP, Denkiewicz NM, Torres NM, Olifiers N, De Lima NDS, de Albuquerque NM, Canassa NF, de Almeida Curi NH, Prestes NP, Falconi N, Gurgel-Filho NM, Pasqualotto N, Cáceres NC, Peroni N, de la Sancha NU, Zanella N, Monroy-Vilchis O, Pays O, Arimoro OA, Ribeiro OS, Villalva P, Gonçalves PR, Santos PM, Brennand P, Rocha P, Akkawi P, Cruz P, Ferreira PM, Prist PR, Martin PS, Arroyo-Gerala P, Auricchio P, Hartmann PA, Antas PTZ, Camargo PHSA, Marinho PH, Ruffino PHP, Prado PI, Martins PW, Cordeiro-Estrela P, Luna P, Sarmento P, Faria Peres PH, Galetti PM Jr, de Castilho PV, Renaud PC, Scarascia PO, Cobra PPA, Lombardi PM, Bessa R, Reyna-Hurtado R, de Souza RCC, Hoogesteijn RJ, Alves RSC, Romagna RS, Silva RL, de Oliveira R, Beltrão-Mendes R, Alencar RM, Coutinho R, da Silva RC, Caribé Grando RLSC, Matos RG, Araujo RDS, Pedroso RF, Durães RMN, Ribeiro RLA, Chagas R, Miotto R, Twardowsky Ramalho Bonikowski R, Muylaert RL, Pagotto RV, Hilário RR, Faria RT, Bassini-Silva R, Sampaio R, Sartorello R, Pires RA, Hatakeyama R, Bianchi RC, Buitenwerf R, Wallace R, Paolino RM, Fusco-Costa R, Trovati RG, Tomasi RJ, Espíndola Hack RO, Magalhães RA, Nobrega RAA, Nobre RA, Massara RL, Fróes RM, Araújo RPDC, León Pérez RR, Jorge RSP, de Paula RC, Martins R, da Cunha RGT, Costa R, Alves RRN, Garcia-Anleu R, Santos Almeida RP, Cueva Loachamín RD, Andrade RS, Juárez R, Bordallo SU, Guaragni SA, Carrillo-Percastegui SE, Seber S, Astete S, Hartz SM, Espinosa S, Álvarez Solas S, Ramos Lima S, Silvestre SM, Machado SAS, Keuroghlian-Eaton S, Albanesi S, Costa SA, Bazilio S, Mendes SL, Althoff SL, Pinheiro SD, Napiwoski SJ, Fernández Ramirez S, Talamoni SA, Age SG, Pereira TC, Moreira TC, Trigo TC, Gondim TMDS, Karlovic TC, Cavalcante T, Maccarini T, Rodrigues TF, de Camargo E Timo TP, Monterrubio TC, Piovezan U, Cavarzere V, Towns V, Onofrio VC, Oliveira VB, Araújo VC, Melo VL, Kanaan VT, Iwakami V, Vale V, Picinatto Filho V, Alberici V, Bastazini VAG, Orsini VS, Braz VDS, Rojas Bonzi VB, Guedes Layme VM, Gaboardi VTR, Rocha VJ, Martins WP, Tomas WM, Hannibal W, Dáttilo W, Silva WR, Endo W, Bercê W, Bravata de la Cruz Y, Ribeiro YGG, Galetti M, and Ribeiro MC
- Subjects
- Animals, Ecosystem, Humans, Canidae, Carnivora, Mustelidae, Ursidae
- Abstract
Mammalian carnivores are considered a key group in maintaining ecological health and can indicate potential ecological integrity in landscapes where they occur. Carnivores also hold high conservation value and their habitat requirements can guide management and conservation plans. The order Carnivora has 84 species from 8 families in the Neotropical region: Canidae; Felidae; Mephitidae; Mustelidae; Otariidae; Phocidae; Procyonidae; and Ursidae. Herein, we include published and unpublished data on native terrestrial Neotropical carnivores (Canidae; Felidae; Mephitidae; Mustelidae; Procyonidae; and Ursidae). NEOTROPICAL CARNIVORES is a publicly available data set that includes 99,605 data entries from 35,511 unique georeferenced coordinates. Detection/non-detection and quantitative data were obtained from 1818 to 2018 by researchers, governmental agencies, non-governmental organizations, and private consultants. Data were collected using several methods including camera trapping, museum collections, roadkill, line transect, and opportunistic records. Literature (peer-reviewed and grey literature) from Portuguese, Spanish and English were incorporated in this compilation. Most of the data set consists of detection data entries (n = 79,343; 79.7%) but also includes non-detection data (n = 20,262; 20.3%). Of those, 43.3% also include count data (n = 43,151). The information available in NEOTROPICAL CARNIVORES will contribute to macroecological, ecological, and conservation questions in multiple spatio-temporal perspectives. As carnivores play key roles in trophic interactions, a better understanding of their distribution and habitat requirements are essential to establish conservation management plans and safeguard the future ecological health of Neotropical ecosystems. Our data paper, combined with other large-scale data sets, has great potential to clarify species distribution and related ecological processes within the Neotropics. There are no copyright restrictions and no restriction for using data from this data paper, as long as the data paper is cited as the source of the information used. We also request that users inform us of how they intend to use the data., (© 2020 The Authors. Ecology © 2020 The Ecological Society of America.)
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- 2020
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23. rSodC is a potential antigen to diagnose Corynebacterium pseudotuberculosis by enzyme-linked immunoassay.
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Farias APF, Rocha Filho JTR, Marchioro SB, Moreira LS, Marques AS, Sá MDCA, Oliveira AADS, Alcântara ME, Mariutti RB, Arni RK, Trindade SC, and Meyer R
- Abstract
Caseous lymphadenitis (CL) is a chronic infectious disease that affects sheep and goats. Many serological tests have been developed to detect the disease; one of the most widely used is the enzyme-linked immunosorbent assay (ELISA), due to its advantages, which include acceptable cost-effectiveness, applicability, sensitivity and specificity. ELISA formulations using recombinant proteins can exhibit significant sensitivity and specificity when using a single purified antigen. DTxR, Trx, TrxR, LexA, SodC, SpaC, NanH, and PknG recombinant proteins can be considered target proteins for ELISA development due to its extracellular or on the cell surface location, which allows a better recognition by the immune system. Therefore, the objectives of this study were to evaluate the antigenic reactivity of Corynebacterium pseudotuberculosis recombinant proteins in goat and sheep serum. Of eight proteins evaluated, rSodC was selected for validation assays with small ruminant serum samples from the semiarid region of the state of Bahia, Brazil. Validation assays with goat serum samples showed that ELISA-rSodC presented sensitivity and specificity of 96% and 94%, respectively. Validation assays with sheep serum showed that ELISA-rSodC exhibited sensitivity and specificity of 95% and 98%, respectively. Analysis of 756 field serum samples showed that rSodC identified 95 positive samples (23%) in goats and 75 positive samples (21%) in sheep. The ELISA with recombinant SodC protein developed in this study discriminated positive and negative serum samples with high levels of sensitivity and specificity. This formulation is promising for epidemiological surveys and CL control programs.Trial registration AEC No 4958051018. 12/18/2018, retrospectively registered.
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- 2020
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24. Artificial neural network employment for element determination in Mugil cephalus by ICP OES in Pontal Bay, Brazil.
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Batista MAS, Santos LN, Chagas BC, Lôbo IP, Novaes CG, Guedes WN, de Jesus RM, Amorim FAC, Pacheco CSV, Moreira LS, and da Silva EGP
- Subjects
- Animals, Brazil, Employment, Humans, Neural Networks, Computer, Bays, Hydrogen Peroxide
- Abstract
Fish are important sources of protein, making them very significant in the human diet. Although the consumption of this food is beneficial for health, it is essential that the product does not contain inorganic components above the limits recommended by the current legislation. Therefore, a method for determination of elements in fish (Mugil cephalus) samples was optimized. A simplex centroid mixture design with restriction was applied for optimization of the acid digestion of samples in an open system under reflux in order to evaluate the best ratio between the reagents HNO
3 , H2 O2 and H2 O. The results indicated that more intense analyte signals were obtained when a mixture containing 3.6 mL of HNO3 (65% v/v), 0.4 mL of H2 O2 (30% v/v) and 6.0 mL of H2 O was used. The accuracy of the method was assessed with a CRM of oyster tissue (NIST 1566b). The method presented relative standard deviations (RSDs) of 3.54%; 3.82%; 4.81% and 3.50% for Zn, Fe, Cu and S, respectively. The detection limits were 0.002 mg kg-1 for Cu and Zn and 0.02 mg kg-1 for Fe and S. The proposed method was applied for the determination of Zn, Fe, Cu and S in fish samples. A Kohonen Self-Organizing Map (KSOM) with K-means implementation was applied to better delimit the boundary between groups and the spatial and temporal influence on how concentrations of the chemical elements were perceived. To verify the separation, the Davies-Bouldin and Silhouette indices were used, obtaining 0.5374 and 0.8541, respectively, indicating satisfactory separation.- Published
- 2020
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25. Reliable measurement of incisal bite force for understanding the control of masticatory muscles.
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Moreira LS, Elias LA, Germer CM, and Palomari ET
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- Adult, Electromyography, Female, Humans, Masseter Muscle, Muscle Contraction, Reproducibility of Results, Temporal Muscle, Bite Force, Masticatory Muscles physiology
- Abstract
Objective: In the present study, we aimed at evaluating the steadiness of incisal bite force during isometric contractions of masticatory muscles., Design: Two separate experiments were carried out in 11 healthy young women. A first experiment was performed to test the reliability of our protocol for measurement of incisal bite force steadiness. The second experiment aimed to evaluate the steadiness of incisal bite force at four submaximal (i.e., percentage of maximum voluntary contraction, MVC) levels (5 %MVC, 10 %MVC, 15 %MVC, and 20 %MVC), along with the bilateral myoelectric activity of two masticatory muscles (temporalis and masseter)., Results: The results from the first experiment showed that our protocol is substantially reliable (intraclass correlation coefficient, ICC > 0.80) for estimating force variability and moderate reliable (0.60 < ICC < 0.80) for estimating spectral properties of force signals. In the second experiment, we found that force standard deviation (SD) increased proportionally to the power of mean force, and coefficient of variation (CoV) was higher at low-intensity contractions and maintained at an approximately constant level for high-intensity contractions. The force-EMG relationships were linear for both muscles at the contraction intensities evaluated in the study (5 %MVC to 20 %MVC), and the median frequency did not change with contraction intensity., Conclusion: Therefore, we presented a reliable method to estimate the incisal bite force, along with additional data on force control and myoelectric activity of jaw elevator muscles during isometric steady contractions., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)
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- 2020
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26. Sinusoidal vibrotactile stimulation differentially improves force steadiness depending on contraction intensity.
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Germer CM, Moreira LS, and Elias LA
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- Adult, Female, Fingers, Humans, Isometric Contraction physiology, Male, Psychomotor Performance, Vibration, Muscle Contraction physiology, Physical Stimulation methods
- Abstract
Studies have reported the benefits of sensory noise in motor performance, but it is not clear if this phenomenon is influenced by muscle contraction intensity. Additionally, most of the studies investigated the role of a stochastic noise on the improvement of motor control and there is no evidence that a sinusoidal vibrotactile stimulation could also enhance motor performance. Eleven participants performed a sensorimotor task while sinusoidal vibrations were applied to the finger skin. The effects of an optimal vibration (OV) on force steadiness were evaluated in different contraction intensities. We assessed the standard deviation (SD) and coefficient of variation (CoV) of force signals. OV significantly decreased force SD irrespective of contraction intensity, but the decrease in force CoV was significantly higher for low-intensity contraction. To the best of our knowledge, our findings are the first evidence that sinusoidal vibrotactile stimulation can enhance force steadiness in a motor task. Also, the significant improvement caused by OV during low-intensity contractions is probably due to the higher sensitivity of the motor system to the synaptic noise. These results add to the current knowledge on the effects of vibrotactile stimulation in motor control and have potential implications for the development of wearable haptic devices. Graphical abstract In this work the effects of a sinusoidal vibrotactile stimulation on force steadiness was investigated. Index finger sensorimotor tasks were performed in three levels of isometric contraction of the FDI muscle: 5, 10 and 15 %MVC. An optimal level of vibration significantly improved force steadiness, but the decrease in force CoV caused by vibration was more pronounced in contractions at 5 %MVC.
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- 2019
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27. Development of procedure for sample preparation of cashew nuts using mixture design and evaluation of nutrient profiles by Kohonen neural network.
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Moreira LS, Chagas BC, Pacheco CSV, Santos HM, de Menezes LHS, Nascimento MM, Batista MAS, de Jesus RM, Amorim FAC, Santos LN, and da Silva EGP
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- Food Analysis statistics & numerical data, Hydrogen Peroxide chemistry, Limit of Detection, Metals analysis, Microwaves, Multivariate Analysis, Neural Networks, Computer, Reproducibility of Results, Spectrum Analysis methods, Trace Elements analysis, Anacardium chemistry, Food Analysis methods, Nuts chemistry
- Abstract
A procedure using ICP OES for sample preparation for the determination of copper, iron and manganese in cashew nuts was developed. Constrained simplex-centroid design was applied in the optimization of the digestion in microwave oven procedure, and the results evaluated from topological maps of the Kohonen network. The best proportion evaluated for the digestion of the sample with HNO
3 , H2 O2 and H2 O was 10:45:45 (%). With optimized conditions, the detection limits were 0.63, 4.3 and 0.37 mg kg-1 , and quantification 2.1, 14 and 1.2 mg kg-1 for Cu, Fe and Mg, respectively. The precision (% RSD) was 1.84, 2.31 and 2.73, for Cu, Fe and Mg, respectively. The procedure proposed had the accuracy confirmed using NIST 1568b (at 95% reliability) and was applied in the samples obtaining concentrations in the range of 10.7-19.4, 44.3-67.2 and 11.0-21.4 mg kg-1 for Cu, Fe and Mg, respectively., (Copyright © 2018 Elsevier Ltd. All rights reserved.)- Published
- 2019
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28. Microwave-assisted digestion using diluted HNO 3 and H 2 O 2 for macro and microelements determination in guarana samples by ICP OES.
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Santos HM, Coutinho JP, Amorim FAC, Lôbo IP, Moreira LS, Nascimento MM, and de Jesus RM
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- Brazil, Carbon analysis, Hydrogen Peroxide chemistry, Metals analysis, Micronutrients analysis, Microwaves, Nitric Acid chemistry, Seeds chemistry, Food Analysis methods, Paullinia chemistry, Spectrum Analysis methods
- Abstract
A microwave-assisted digestion procedure using diluted HNO
3 and H2 O2 was developed for multi-element determination in guarana samples by ICP OES. Optimization step was performed employing a mixture design with pseudocomponents using HNO3 , H2 O2 and H2 O. The analytical signal of each element, residual acidity and residual carbon content were optimized simultaneously using the desirability function. The best condition for digestion of a 250 mg sample mass resulted from a mixture constituted by 1.0 mL of HNO3 , 3.0 mL of H2 O2 and 6.0 mL of H2 O. This condition allowed final digests with residual acidity and residual carbon content of 0.4 mol L-1 and 6.5%, respectively. The method was validated and applied for the determination of K, Ca, Mg, S, P, Cu, Fe, Mn and Zn in 72 guarana seed samples from Bahia state. This work presents unpublished results about the mineral composition of guarana seed samples produced in Bahia state, Brazil., (Copyright © 2017 Elsevier Ltd. All rights reserved.)- Published
- 2019
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29. Heteronuclear molecules from matrix isolation sublimation and atomic diffusion.
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Oliveira AN, Sacramento RL, Moreira LS, Azevedo LOA, Wolff W, and Lenz Cesar C
- Abstract
We demonstrate the production of cryogenic beams of heteronuclear molecules from the matrix isolation sublimation (MISu) technique. A sapphire mirror serves as a substrate whereupon a solid Ne matrix is grown. Atoms of Li, H, Ca, and C are implanted into the matrix via subsequent laser ablation of different solid precursors such as Ca, Li, LiH, and graphite. The matrix is sublimated into vacuum generating a cryogenic beam of Ne carrying the previously isolated neutral atomic and molecular species. A compact and low energy electron source and time-of-flight mass spectrometer was designed to fit this system at low temperature. With electron ionization time-of-flight mass spectrometry, we analyze the species coming from MISu and demonstrate the formation of heteronuclear molecules in the matrix. In this first study, we produced LiCa from the sequential implantation of Li and Ca into the matrix and some clusters of C
n Lim after Li and C ablation. Also from ablation of a single LiH pellet, we observed clusters of Lin Hm . This novel technique opens up the opportunity to generate cryogenic beams of different molecules for precision physics and chemistry studies. Laser or microwave high resolution spectroscopy of a molecular beam benefits from low translational and rovibrational temperatures and forward velocities, such as the ones produced in this technique. Toward the prospect of enhancing the molecular formation, we introduce a new method to study the atomic diffusion of Li and Ca in the Ne matrix via laser spectroscopy during sublimation. We estimate a small diffusion coefficient at 7 K, but a surprisingly linear atomic dispersion during sublimation. The method is extensive to other species and matrices.- Published
- 2018
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30. Anti-Inflammatory and Antioxidant Actions of Methyl Jasmonate Are Associated with Metabolic Modifications in the Liver of Arthritic Rats.
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Sá-Nakanishi AB, Soni-Neto J, Moreira LS, Gonçalves GA, Silva FMS, Bracht L, Bersani-Amado CA, Peralta RM, Bracht A, and Comar JF
- Subjects
- Acetates pharmacology, Animals, Anti-Inflammatory Agents pharmacology, Antioxidants pharmacology, Arthritis, Rheumatoid pathology, Cyclopentanes pharmacology, Oxidative Stress, Oxylipins pharmacology, Rats, Acetates therapeutic use, Anti-Inflammatory Agents therapeutic use, Antioxidants therapeutic use, Arthritis, Rheumatoid drug therapy, Cyclopentanes therapeutic use, Liver pathology, Oxylipins therapeutic use
- Abstract
Methyl jasmonate (MeJA) is a fatty acid-derived cyclopentanone which shares structural similarities with prostaglandins and has been under study as a promising anti-inflammatory agent. This study investigated the actions of MeJA on systemic inflammation and oxidative status in rats with adjuvant-induced arthritis, a model for rheumatoid arthritis. MeJA (75 to 300 mg·kg
-1 ) was administrated orally during 18 days after arthritis induction with Freund's adjuvant. Articular and systemic inflammation was greatly increased in arthritic rats, likewise the oxidative stress in plasma and liver. The hepatic glucokinase activity and glycolysis were increased in arthritic rats. MeJA decreased most inflammatory parameters and abolished the increased protein carbonylation in plasma and liver, diminished the increased hepatic ROS content, and restored the hepatic GSH/GSSG ratio in arthritic rats. However, the MeJA treatment decreased the hepatic glucokinase activity and glycolysis and stimulated mitochondrial ROS production in healthy and arthritic rats. Oxygen uptake was increased by MeJA only in livers from treated arthritic rats. This action may bear relation to the increased activity of mitochondrial NADP+ -dependent enzymes to provide reducing equivalents for the glutathione cycle. These beneficial effects, however, are associated with a decreased glucose flux through the glycolysis in the liver of arthritic and healthy rats.- Published
- 2018
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31. Ethyl-acetate fraction of Trichilia catigua protects against oxidative stress and neuroinflammation after cerebral ischemia/reperfusion.
- Author
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Godinho J, de Sa-Nakanishi AB, Moreira LS, de Oliveira RMW, Huzita CH, Mello JCP, da Silva AOF, Nakamura CV, Previdelli IS, Ribeiro MHDM, and Milani H
- Subjects
- Acetates chemistry, Animals, Anti-Inflammatory Agents pharmacology, Antioxidants pharmacology, Brain Ischemia metabolism, CD11b Antigen metabolism, Catalase metabolism, Glial Fibrillary Acidic Protein metabolism, Glutathione metabolism, Hippocampus drug effects, Hippocampus metabolism, Male, Neuroprotective Agents pharmacology, Oxidative Stress drug effects, Phytotherapy, Plant Extracts pharmacology, Plant Stems chemistry, Rats, Wistar, Reperfusion Injury metabolism, Solvents chemistry, Superoxide Dismutase metabolism, Anti-Inflammatory Agents therapeutic use, Antioxidants therapeutic use, Brain Ischemia drug therapy, Meliaceae, Neuroprotective Agents therapeutic use, Plant Extracts therapeutic use, Reperfusion Injury drug therapy
- Abstract
Ethnopharmacological Relevance: Trichilia catigua A. Juss (Meliaceae) preparations have been used in folk medicine to alleviate fatigue, stress, and improve memory. Antinociceptive, antiinflammatory, and in vitro neuroprotective effects have been observed in animals. Cerebral ischemia/reperfusion (I/R) leads to severe neuropsychological deficits that are largely associated with oxidative stress, inflammation and neurodegeneration. We reported previously that an ethyl-acetate fraction (EAF) of T. catigua reduced brain ischemia-induced learning and memory impairments in the absence of histological protection., Aim of the Study: Continuing those studies, here we aimed to investigate the antioxidant and antiinflammatory properties of T. catigua in an in vivo model of I/R., Material and Methods: Rats were subjected to 15 min of brain ischemia (4-VO model) followed by up to 15 days of reperfusion. Vehicle was given by gavage 30 min before ischemia and at 1 h of reperfusion. In a first experiment, brain ischemia-induced changes in oxidative stress markers, i.e., reduced glutathione (GSH), oxidized glutathione (GSSG), superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), and protein carbonyl groups (PCGs) were measured on days 1, 3, and 5 post-ischemia. Similar time course analysis was done for neuroinflammation markers, i.e., microglia (OX42 immunorreactivity) and astrocytes (GFAP immunorreactivity), in the hippocampus. In a second experiment, the time points at which these markers of oxidative stress and neuroinflammation peaked were used to test the effects of T. catigua (400 mg/kg, p.o.)., Results: Oxidative stress markers peaked on day 1 post-ischemia. GSH decreased (-23.2%) while GSSG increased (+ 71.1%), which yielded a significant reduction in the GSH/GSSG ratio (-39.1%). The activity of CAT was largely reduced by ischemia (-54.6% to -65.1%), while the concentration of PCG almost doubled in the brain of ischemic rats (+99.10%) in comparison to sham. Treatment with the EAF of T. catigua normalized these changes in oxidative markers to the control levels (GSH: +27.5%; GSSG: -23.8%; GSH/GSSG: +44.6%; PCG: -80.3%). In the hippocampus, neuroinflammation markers peaked on day 5 post-ischemia, with microglial and astrocytic responses increasing to 54.8% and 37.1%, respectively. The elevation in glial cells response was completely prevented by EAF., Conclusion: These results demonstrate that T. catigua has both antioxidant and antiinflammatory activities after transient global cerebral ischemia in rats, which may contribute to the previously reported memory protective effect of T. catigua., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2018
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32. Dual effect of silymarin on experimental non-alcoholic steatohepatitis induced by irinotecan.
- Author
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Marcolino Assis-Júnior E, Melo AT, Pereira VBM, Wong DVT, Sousa NRP, Oliveira CMG, Malveira LRC, Moreira LS, Souza MHLP, Almeida PRC, and Lima-Júnior RCP
- Subjects
- Animals, Bacterial Infections drug therapy, Bacterial Infections pathology, Biomarkers metabolism, Camptothecin toxicity, Cytokines metabolism, Female, Irinotecan, Lipid Metabolism drug effects, Liver microbiology, Liver pathology, Liver Function Tests, Mice, Neutrophil Infiltration, Non-alcoholic Fatty Liver Disease pathology, Oxidative Stress drug effects, Sulfhydryl Compounds metabolism, Anti-Inflammatory Agents, Non-Steroidal therapeutic use, Antineoplastic Agents, Phytogenic toxicity, Camptothecin analogs & derivatives, Non-alcoholic Fatty Liver Disease chemically induced, Non-alcoholic Fatty Liver Disease drug therapy, Silymarin therapeutic use
- Abstract
Irinotecan-based regimens are commonly used for treatment of colorectal cancer, which is limited by mucositis and non-alcoholic steatohepatitis (NASH). Silymarin (SIL) prevents fatty liver disease in the clinical setting and in models of liver damage induced chemically. This study investigated the possible effect of SIL on irinotecan (IRI)-induced NASH. Swiss female mice were injected with saline (SAL 5ml/kg i.p.), IRI (50mg/kg i.p.), SIL (150mg/kg p.o.) or IRI (50mg/kg i.p.)+(SIL 1.5, 15 or 150mg/kg p.o.) thrice/week/7weeks. On the seventh week, blood samples were collected for transaminases assay and livers were collected for histopathology, measurement of the total lipids, malondyadehyde (MDA), non-protein sulfhydryl groups (NPSH), cytokines (IL-1β, IL 6 and IL-10), 3-nitrotyrosine (N-Tyr) and toll-like receptor 4 (TLR4) immunoexpression, quantification of NF-kB, α-smooth muscle actin (α-SMA), and Escherichia coli 16S rRNA gene (RRS) expression. IRI increased liver transaminases, neutrophil infiltration, lipid accumulation, MDA, IL-1β and IL-6 levels, N-Tyr and TLR4 immunostaining, NF-kB, α-SMA expression and RRS versus the SAL group (p<0.05). Additionally, SIL (1.5mg/kg) improved these parameters (p<0.05), except neutrophil infiltration and RSS versus the IRI group. Furthermore, the SIL (15mg/kg) only improved the inflammatory parameters, the expression of α-SMA and RRS versus the IRI group (p<0.05). The higher dose of SIL (150mg/kg) was even more deleterious than the intermediate dose. Therefore, silymarin showed a dual effect on liver damage induced by IRI. Hepatoprotection seems to involve the inhibition of oxidative stress and protein nitrosylation, preventing activation of hepatic fibrosis mechanisms., (Copyright © 2017 Elsevier Inc. All rights reserved.)
- Published
- 2017
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33. Cryogenic mount for mirror and piezoelectric actuator for an optical cavity.
- Author
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Oliveira AN, Moreira LS, Sacramento RL, Kosulic L, Brasil VB, Wolff W, and Cesar CL
- Abstract
We present the development of a mount that accommodates a mirror and a piezoelectric actuator with emphasis on physical needs for low temperature operation. The design uses a monolithic construction with flexure features that allow it to steadily hold the mirror and the piezoelectric actuator without glue and accommodate differential thermal contraction. The mount is small and lightweight, adding little heat capacity and inertia. It provides a pre-loading of the piezoelectric actuator as well as a good thermal connection to the mirror and a thermal short across the piezoelectric actuator. The performance of the assemblies has been tested by thermally cycling from room temperature down to 3 K more than a dozen times and over one hundred times to 77 K, without showing any derating. Such mounts are proposed for the cryogenic optical enhancement cavities of the ALPHA experiment at CERN for laser spectroscopy of antihydrogen and for hydrogen spectroscopy in our laboratory at UFRJ.
- Published
- 2017
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34. A longitudinal assessment of myoelectric activity, postural sway, and low-back pain during pregnancy.
- Author
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Moreira LS, Elias LA, Gomide AB, Vieira MF, and DO Amaral WN
- Subjects
- Adult, Computer Simulation, Female, Humans, Longitudinal Studies, Pregnancy, Reproducibility of Results, Sensitivity and Specificity, Aging, Low Back Pain physiopathology, Models, Biological, Muscle Contraction, Muscle, Skeletal physiopathology, Postural Balance, Pregnancy Complications physiopathology
- Abstract
Purpose: The present study aimed at investigating the control of upright quiet standing in pregnant women throughout pregnancy, and whether low-back pain exerts influence on this motor task., Methods: Myoelectric signals from postural muscles and stabilometric data were collected from 15 non-pregnant and 15 pregnant women during upright quiet standing. Electromyogram envelopes and center of pressure metrics were evaluated in the control group, as well as in pregnant women in their first and third trimester of pregnancy. A correlation analysis was performed between the measured variables and a low-back pain disability index., Results: Pregnant women exhibited a decreased maximum voluntary isometric activity for all postural muscles evaluated. Additionally, the activity of lumbar muscles during the postural task was significantly higher in the pregnant women in comparison to the non-pregnant controls. The soleus muscle maintained its activity at the same level as the gestation progressed. Higher postural oscillations were observed in the anteroposterior direction while mediolateral sway was reduced in the third trimester of pregnancy. No correlation was detected between the lowback pain disability index and neuromechanical variables., Conclusion: This study provides additional data regarding the functioning and adaptations of the postural control system during pregnancy. Also, we provide further evidence that postural control during quiet standing cannot be used to predict the occurrence of low-back pain. We hypothesize that the modifications in the neural drive to the muscles, as well as in postural sway may be related to changes in the biomechanics and hormonal levels experienced by the pregnant women.
- Published
- 2017
35. Bioinspired seeding of biomaterials using three dimensional microtissues induces chondrogenic stem cell differentiation and cartilage formation under growth factor free conditions.
- Author
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Leijten J, Teixeira LS, Bolander J, Ji W, Vanspauwen B, Lammertyn J, Schrooten J, and Luyten FP
- Subjects
- Animals, Biomarkers metabolism, Cartilage drug effects, Cell Aggregation drug effects, Cell Nucleus drug effects, Cell Nucleus metabolism, Chondrogenesis genetics, Gene Expression Regulation drug effects, Humans, Mice, Periosteum cytology, Protein Transport drug effects, SOX9 Transcription Factor metabolism, Stem Cells cytology, Transcription, Genetic drug effects, Transforming Growth Factor beta pharmacology, Biocompatible Materials pharmacology, Biomimetics methods, Cartilage growth & development, Cell Differentiation drug effects, Chondrogenesis drug effects, Intercellular Signaling Peptides and Proteins pharmacology, Tissue Engineering methods
- Abstract
Cell laden biomaterials are archetypically seeded with individual cells and steered into the desired behavior using exogenous stimuli to control growth and differentiation. In contrast, direct cell-cell contact is instructive and even essential for natural tissue formation. Namely, microaggregation and condensation of mesenchymal progenitor cells triggers chondrogenesis and thereby drives limb formation. Yet a biomimetic strategy translating this approach into a cell laden biomaterial-based therapy has remained largely unexplored. Here, we integrate the microenvironment of cellular condensation into biomaterials by encapsulating microaggregates of a hundred human periosteum-derived stem cells. This resulted in decreased stemness-related markers, up regulation of chondrogenic genes and improved in vivo cartilage tissue formation, as compared to single cell seeded biomaterials. Importantly, even in the absence of exogenous growth factors, the microaggregate laden hydrogels outperformed conventional single cell laden hydrogels containing supraphysiological levels of the chondrogenic growth factor TGFB. Overall, the bioinspired seeding strategy described herein represents an efficient and growth factor-free approach to efficiently steer cell fate and drive tissue formation for biomaterial-based tissue engineering strategies.
- Published
- 2016
- Full Text
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36. Geographic comparison of plant genera used in frugivory among the pitheciids Cacajao, Callicebus, Chiropotes, and Pithecia.
- Author
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Boyle SA, Thompson CL, Deluycker A, Alvarez SJ, Alvim TH, Aquino R, Bezerra BM, Boubli JP, Bowler M, Caselli CB, Chagas RR, Ferrari SF, Fontes IP, Gregory T, Haugaasen T, Heiduck S, Hores R, Lehman S, Melo FR, Moreira LS, Moura VS, Nagy-Reis MB, Palacios E, Palminteri S, Peres CA, Pinto L, Port-Carvalho M, Rodríguez A, Santos RR, Setz EZ, Shaffer CA, Silva FE, Silva RF, Souza-Alves JP, Trevelin LC, Veiga LM, Vieira TM, DuBose ME, and Barnett AA
- Subjects
- Animals, Ecosystem, Forests, Geography, Phylogeography, Diet veterinary, Fruit classification, Herbivory, Pitheciidae physiology, Plants classification
- Abstract
Pitheciids are known for their frugivorous diets, but there has been no broad-scale comparison of fruit genera used by these primates that range across five geographic regions in South America. We compiled 31 fruit lists from data collected from 18 species (three Cacajao, six Callicebus, five Chiropotes, and four Pithecia) at 26 study sites in six countries. Together, these lists contained 455 plant genera from 96 families. We predicted that 1) closely related Chiropotes and Cacajao would demonstrate the greatest similarity in fruit lists; 2) pitheciids living in closer geographic proximity would have greater similarities in fruit lists; and 3) fruit genus richness would be lower in lists from forest fragments than continuous forests. Fruit genus richness was greatest for the composite Chiropotes list, even though Pithecia had the greatest overall sampling effort. We also found that the Callicebus composite fruit list had lower similarity scores in comparison with the composite food lists of the other three genera (both within and between geographic areas). Chiropotes and Pithecia showed strongest similarities in fruit lists, followed by sister taxa Chiropotes and Cacajao. Overall, pitheciids in closer proximity had more similarities in their fruit list, and this pattern was evident in the fruit lists for both Callicebus and Chiropotes. There was no difference in the number of fruit genera used by pitheciids in habitat fragments and continuous forest. Our findings demonstrate that pitheciids use a variety of fruit genera, but phylogenetic and geographic patterns in fruit use are not consistent across all pitheciid genera. This study represents the most extensive examination of pitheciid fruit consumption to date, but future research is needed to investigate the extent to which the trends in fruit genus richness noted here are attributable to habitat differences among study sites, differences in feeding ecology, or a combination of both., (© 2015 Wiley Periodicals, Inc.)
- Published
- 2016
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37. [Public drinking water supply and egg laying by Aedes aegypti].
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Marques GR, Chaves LS, Serpa LL, Arduíno Mde B, and Chaves FJ
- Subjects
- Animals, Drinking Water parasitology, Female, Household Articles, Insect Vectors growth & development, Larva growth & development, Aedes anatomy & histology, Drinking Water analysis, Mosquito Control methods, Oviposition physiology, Water Pollutants, Chemical analysis, Water Quality, Water Supply analysis
- Abstract
Objective: To evaluate the effect of the quality of publicly supplied water in domestic water tanks on egg laying by female Aedes aegypti., Methods: Laboratory study on immature Ae. aegypti, collected from water-tanks in the municipality of Potim, SP, Southeastern Brazil. Each cage contained three types of water in which eggs could be laid: Three choice per test were simultaneously used to deposit the eggs, ovipositor (A) with water collected from a water tank in Taubaté, ovipositor (B) with distilled water (control) and ovipositor (C) water collected from a water tank in the municipality of Potim. Physiochemical parameters were analyzed. The Kruskall-Wallis test was used to analyze the mean number of eggs in each water sample and the Dwass-Steel-Critchlow-Fligner test was used in making comparisons. To evaluate egg laying, an ovipositional activity index was adopted., Results: A significant difference in the number of eggs was found between the liquid solutions tested (H = 45; p < 0.0001). The number of eggs found in water tank samples originating from deep wells (C), was statistically superior to water samples from water tanks originating from superficial wells (A) (p < 0.0001) and from the Control (C) p < 0.0001. There was no significant difference between the number of eggs in Control (B) and the surface water (A). In all three tests, the first position was the most productive in all tested solutions. Only water sample (C) produced a positive index (0.54), i.e., attractive to egg laying., Conclusions: Water quality influences egg laying by Aedes aegypti. The high concentrations of ammonium nitrate in public water supplies suggest that this chemical component was responsible for attracting pregnant female Aedes aegypti to lay eggs in these environments.
- Published
- 2013
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38. Terrestrial activity in pitheciins (Cacajao, Chiropotes, and Pithecia).
- Author
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Barnett AA, Boyle SA, Norconk MM, Palminteri S, Santos RR, Veiga LM, Alvim TH, Bowler M, Chism J, DI Fiore A, Fernandez-Duque E, Guimarães AC, Harrison-Levine A, Haugaasen T, Lehman S, Mackinnon KC, DE Melo FR, Moreira LS, Moura VS, Phillips CR, Pinto LP, Port-Carvalho M, Setz EZ, Shaffer C, DA Silva LR, DA Silva SD, Soares RF, Thompson CL, Vieira TM, Vreedzaam A, Walker-Pacheco SE, Spironello WR, Maclarnon A, and Ferrari SF
- Subjects
- Animals, Female, Male, Predatory Behavior, Seasons, South America, Behavior, Animal, Ecosystem, Pitheciidae
- Abstract
Neotropical monkeys of the genera Cacajao, Chiropotes, and Pithecia (Pitheciidae) are considered to be highly arboreal, spending most of their time feeding and traveling in the upper canopy. Until now, the use of terrestrial substrates has not been analyzed in detail in this group. Here, we review the frequency of terrestrial use among pitheciin taxa to determine the ecological and social conditions that might lead to such behavior. We collated published and unpublished data from 14 taxa in the three genera. Data were gleaned from 53 published studies (including five on multiple pitheciin genera) and personal communications of unpublished data distributed across 31 localities. Terrestrial activity was reported in 61% of Pithecia field studies (11 of 18), in 34% of Chiropotes studies (10 of 29), and 36% of Cacajao studies (4 of 11). Within Pithecia, terrestrial behavior was more frequently reported in smaller species (e.g. P. pithecia) that are vertical clingers and leapers and make extensive use of the understory than in in the larger bodied canopy dwellers of the western Amazon (e.g. P. irrorata). Terrestrial behavior in Pithecia also occurred more frequently and lasted longer than in Cacajao or Chiropotes. An apparent association was found between flooded habitats and terrestrial activity and there is evidence of the development of a "local pattern" of terrestrial use in some populations. Seasonal fruit availability also may stimulate terrestrial behavior. Individuals also descended to the ground when visiting mineral licks, escaping predators, and responding to accidents such as a dropped infant. Overall, the results of this review emphasize that terrestrial use is rare among the pitheciins in general and is usually associated with the exploitation of specific resources or habitat types., (© 2012 Wiley Periodicals, Inc.)
- Published
- 2012
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39. Enzyme-catalyzed crosslinkable hydrogels: emerging strategies for tissue engineering.
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Teixeira LS, Feijen J, van Blitterswijk CA, Dijkstra PJ, and Karperien M
- Subjects
- Animals, Humans, Biocatalysis drug effects, Cross-Linking Reagents metabolism, Enzymes metabolism, Hydrogels pharmacology, Tissue Engineering methods
- Abstract
State-of-the-art bioactive hydrogels can easily and efficiently be formed by enzyme-catalyzed mild-crosslinking reactions in situ. Yet this cell-friendly and substrate-specific method remains under explored. Hydrogels prepared by using enzyme systems like tyrosinases, transferases and lysyl oxidases show interesting characteristics as dynamic scaffolds and as systems for controlled release. Increased attention is currently paid to hydrogels obtained via crosslinking of precursors by transferases or peroxidases as catalysts. Enzyme-mediated crosslinking has proven its efficiency and attention has now shifted to the development of enzymatically crosslinked hydrogels with higher degrees of complexity, mimicking extracellular matrices. Moreover, bottom-up approaches combining biocatalysts and self-assembly are being explored for the development of complex nano-scale architectures. In this review, the use of enzymatic crosslinking for the preparation of hydrogels as an innovative alternative to other crosslinking methods, such as the commonly used UV-mediated photo-crosslinking or physical crosslinking, will be discussed. Photo-initiator-based crosslinking may induce cytotoxicity in the formed gels, whereas physical crosslinking may lead to gels which do not have sufficient mechanical strength and stability. These limitations can be overcome using enzymes to form covalently crosslinked hydrogels. Herewith, we report the mechanisms involved and current applications, focusing on emerging strategies for tissue engineering and regenerative medicine., (Copyright © 2011 Elsevier Ltd. All rights reserved.)
- Published
- 2012
- Full Text
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40. Wound infection by multiresistant Staphylococcus sciuri identified by molecular methods.
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Coimbra DG, Almeida AG, Jùnior JB, da Silva LA, Pimentel BJ, Gitaì DL, Moreira LS, Silva-Filho EA, and de Andrade TG
- Subjects
- Adult, Amputation, Surgical, Anti-Bacterial Agents pharmacology, Chloramphenicol pharmacology, Humans, Male, Methicillin-Resistant Staphylococcus aureus drug effects, Methicillin-Resistant Staphylococcus aureus genetics, RNA, Ribosomal, 16S genetics, Staphylococcal Infections drug therapy, Staphylococcal Infections surgery, Vancomycin pharmacology, Wounds and Injuries surgery, Drug Resistance, Bacterial genetics, Drug Resistance, Multiple genetics, Methicillin-Resistant Staphylococcus aureus isolation & purification, Staphylococcal Infections microbiology, Wounds and Injuries microbiology
- Abstract
We describe a case of wound infection by multidrug-resistant Staphylococcus sciuri in a patient admitted to hospital for injuries in Agreste Alagoas, Brazil, identified through broad-spectrum PCR and sequencing of 16S rDNA gene. Due to its high resistance profile, the infection was characterized as methicillin-resistant Staphylococcus presenting sensitive only to vancomycin and chloramphenicol. The injury resulting from trauma associated with infection resulted in amputation of the infected limb.
- Published
- 2011
41. Enzymatically-crosslinked injectable hydrogels based on biomimetic dextran-hyaluronic acid conjugates for cartilage tissue engineering.
- Author
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Jin R, Teixeira LS, Dijkstra PJ, van Blitterswijk CA, Karperien M, and Feijen J
- Subjects
- Animals, Biocompatible Materials chemistry, Carbohydrate Conformation, Carbohydrate Sequence, Cartilage pathology, Cattle, Cell Proliferation, Cells, Cultured, Chondrocytes chemistry, Chondrocytes cytology, Cross-Linking Reagents chemistry, Extracellular Matrix chemistry, Extracellular Matrix metabolism, Injections, Materials Testing, Models, Molecular, Molecular Sequence Data, Sympathomimetics chemistry, Tissue Scaffolds chemistry, Tyramine chemistry, Biomimetics, Cartilage chemistry, Dextrans chemistry, Hyaluronic Acid chemistry, Hydrogels chemistry, Tissue Engineering instrumentation, Tissue Engineering methods
- Abstract
Polysaccharide hybrids consisting of hyaluronic acid (HA) grafted with a dextran-tyramine conjugate (Dex-TA) were synthesized and investigated as injectable biomimetic hydrogels for cartilage tissue engineering. The design of these hybrids (denoted as HA-g-Dex-TA) is based on the molecular structure of proteoglycans present in the extracellular matrix of native cartilage. Hydrogels of HA-g-Dex-TA were rapidly formed within 2 min via enzymatic crosslinking of the tyramine residues in the presence of horseradish peroxidase and hydrogen peroxide. The gelation time, equilibrium swelling and storage modulus could be adjusted by varying the degree of substitution of tyramine residues and polymer concentration. Bovine chondrocytes incorporated in the HA-g-Dex-TA hydrogels remained viable, as shown by the Live-dead assay. Moreover, enhanced chondrocyte proliferation and matrix production were observed in the HA-g-Dex-TA hydrogels compared to Dex-TA hydrogels. These results suggest that HA-g-Dex-TA hydrogels have a high potential as injectable scaffolds for cartilage tissue engineering., (Copyright 2010 Elsevier Ltd. All rights reserved.)
- Published
- 2010
- Full Text
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42. Hypertonic environment elicits cyclooxygenase-2-driven prostaglandin E2 generation by colon cancer cells: role of cytosolic phospholipase A2-alpha and kinase signaling pathways.
- Author
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Gentile LB, Piva B, Capizzani BC, Furlaneto LG, Moreira LS, Zamith-Miranda D, and Diaz BL
- Subjects
- Caco-2 Cells, Humans, Immunoblotting, Immunoenzyme Techniques, Mitogen-Activated Protein Kinase 1 antagonists & inhibitors, Mitogen-Activated Protein Kinase 1 metabolism, Mitogen-Activated Protein Kinase 3 antagonists & inhibitors, Mitogen-Activated Protein Kinase 3 metabolism, Signal Transduction drug effects, p38 Mitogen-Activated Protein Kinases antagonists & inhibitors, p38 Mitogen-Activated Protein Kinases metabolism, Cyclooxygenase 2 metabolism, Dinoprostone metabolism, Group IV Phospholipases A2 metabolism, Hypertonic Solutions pharmacology
- Abstract
Cyclooxygenase (COX)-2-derived prostaglandin (PG)E(2) controls many aspects of colon cancer development, modulating from apoptosis resistance and cell proliferation to angiogenesis, invasion, and metastasis. Here, we investigated the role of different phospholipases (PL)A(2) in supplying arachidonic acid (AA) for COX-2-dependent PGE(2) generation and signaling pathways involved in activation of colon cancer cells by a physiologically relevant stimulus. To emulate the hypertonic environment found physiologically in colon, the human colon cancer cell line Caco-2 was maintained in hypertonic complete DMEM medium. Human colon cancer cell line Caco-2 exposed to a hypertonic environment responded with marked AA release, COX-2 induction and PGE(2) generation. Selective secretory (s)PLA(2) and calcium-independent (i)PLA(2) inhibitors did not modify PGE(2) generation, while either COX-2 or cytosolic (c)PLA(2) inhibitors completely inhibited PGE(2) generation. cPLA(2)-alpha was responsible for AA supply for PGE(2) generation, but had no role in COX-2 induction. Mitogen-activated protein (MAP) kinases, ERK 1/2, p38, and JNK, participated in the signaling events that lead to PGE(2) generation by modulating AA release, but only ERK 1/2 was involved in COX-2 upregulation. Our results indicate that hypertonic stress activates PGE(2) generation by Caco-2 cells through a mechanism dependent on MAP kinase-regulated AA mobilization, increased cPLA(2)-alpha activity, and COX-2 induction., (Copyright 2009 Elsevier Ltd. All rights reserved.)
- Published
- 2010
- Full Text
- View/download PDF
43. Cytosolic phospholipase A2-driven PGE2 synthesis within unsaturated fatty acids-induced lipid bodies of epithelial cells.
- Author
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Moreira LS, Piva B, Gentile LB, Mesquita-Santos FP, D'Avila H, Maya-Monteiro CM, Bozza PT, Bandeira-Melo C, and Diaz BL
- Subjects
- Animals, Cells, Cultured, Cytoplasm metabolism, Epithelial Cells metabolism, Immunoblotting, Intestinal Mucosa cytology, Lipids chemistry, Protein Kinase C metabolism, Rats, Signal Transduction, p38 Mitogen-Activated Protein Kinases metabolism, Arachidonic Acid metabolism, Arachidonic Acid pharmacology, Dinoprostone biosynthesis, Epithelial Cells drug effects, Lipid Metabolism, Oleic Acid pharmacology, Phospholipases A2, Cytosolic metabolism
- Abstract
Cytoplasmic lipid bodies (also known as lipid droplets) are intracellular deposits of arachidonic acid (AA), which can be metabolized for eicosanoid generation. PGE2 is a major AA metabolite produced by epithelial cells and can modulate restoration of epithelium homeostasis after injury. We studied lipid body biogenesis and their role in AA metabolic pathway in an epithelial cell line derived from normal rat intestinal epithelium, IEC-6 cells. Lipid bodies were virtually absent in confluent IEC-6 cells. Stimulation of confluent IEC-6 cells with unsaturated fatty acids, including AA or oleic acid (OA), induced rapid lipid body assembly that was independent on its metabolism to PGE(2), but dependent on G-coupled receptor-driven signaling through p38, PKC, and PI3 K. Newly formed lipid bodies compartmentalized cytosolic phospholipase (cPL)A(2)-alpha, while facilitated AA mobilization and synthesis of PGE(2) within epithelial cells. Thus, both lipid body-related events, including highly regulated biogenesis and functional assembly of cPLA (2)-alpha-driven enhanced AA mobilization and PGE(2)production, may have key roles in epithelial cell-driven inflammatory functions, and may represent relevant therapeutic targets of epithelial pathologies.
- Published
- 2009
- Full Text
- View/download PDF
44. A study of the activity of 2-(alkylamino)-1-phenyl-1-ethanethiosulfuric acids against infection by Schistosoma mansoni in a murine model.
- Author
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Moreira LS, Piló-Veloso D, de Mello RT, Coelho PM, and Nelson DL
- Subjects
- Animals, Disease Models, Animal, Drug Evaluation, Preclinical, Female, Male, Mice, Schistosoma mansoni drug effects, Sex Factors, Treatment Outcome, Anthelmintics therapeutic use, Schistosomiasis mansoni drug therapy, Sulfuric Acid Esters therapeutic use
- Abstract
The schistosomicidal activities of seven 2-(alkylamino)-1-phenyl-1-ethanethiosulfuric acids (1a-g) (R=propyl, isopropyl, butyl, isobutyl, t-butyl, sec-butyl, cyclohexyl, respectively) were determined in female Swiss mice infected with Schistosoma mansoni. The compounds were administered in a single oral dose of 800 mg/kg to groups of 15 mice infected with 50 cercariae each. All the compounds were found to be active, a high animal mortality being observed with 1e. These compounds have a high specificity against female worms (64-100% reduction vs. 33-61% reduction in male worms). The test was repeated, a 400-mg/kg sub-dose of 1f also being tested, and similar results were observed. A 94% reduction in the number of female worms was observed when compound 1c was administered in a single 800-mg/kg dose to animals infected with 80 cercariae. Finally, the test was repeated with single 800 mg/kg oral doses of compounds 1e (highly purified) and 1f and a 400-mg/kg sub-dose of 1c. The toxicity of 1e was confirmed, while the animals that received 1c and 1f presented reductions in the worm loads corresponding to 45.9% (male worms) and 84.8% (female worms) for 1c and 50.4% (male worms) and 94.2% (female worms) for 1f.
- Published
- 2007
- Full Text
- View/download PDF
45. Identification of novel candidate genes for globin regulation in erythroid cells containing large deletions of the human beta-globin gene cluster.
- Author
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de Andrade TG, Peterson KR, Cunha AF, Moreira LS, Fattori A, Saad ST, and Costa FF
- Subjects
- DNA-Binding Proteins metabolism, Female, Fetal Hemoglobin genetics, Gene Expression Profiling, Gene Library, Homeodomain Proteins metabolism, Humans, Kruppel-Like Transcription Factors, Male, Nuclear Proteins, Nucleic Acid Hybridization methods, Proteins metabolism, RNA, Messenger genetics, Reverse Transcriptase Polymerase Chain Reaction methods, Sensitivity and Specificity, Sequence Deletion, Thalassemia genetics, Transcription Factors metabolism, Transcription, Genetic, DNA-Binding Proteins genetics, Erythroid Cells metabolism, Globins genetics, Globins metabolism, Homeodomain Proteins genetics, Multigene Family, Proteins genetics, Transcription Factors genetics
- Abstract
The genetic mechanisms underlying the continued expression of the gamma-globin genes during the adult stage in deletional hereditary persistence of fetal hemoglobin (HPFH) and deltabeta-thalassemias are not completely understood. Herein, we investigated the possible involvement of transcription factors, using the suppression subtractive hybridization (SSH) method as an initial screen to identify differentially expressed transcripts in reticulocytes from a normal and a HPFH-2 subject. Some of the detectable transcripts may participate in globin gene regulation. Quantitative real-time PCR (qRT-PCR) experiments confirmed the downregulation of ZHX2, a transcriptional repressor, in two HPFH-2 subjects and in a carrier of the Sicilian deltabeta-thalassemia trait. The chromatin remodeling factors ARID1B and TSPYL1 had a very similar pattern of expression with an incremental increase in HPFH and decreased expression in deltabeta-thalassemia. These differences suggest a mechanism to explain the heterocellular and pancellular distribution of fetal hemoglobin in deltabeta-thalassemia and deletional HPFH, respectively. Interestingly, alpha-globin mRNA levels were decreased, similar to beta-globin in all reticulocyte samples analyzed.
- Published
- 2006
- Full Text
- View/download PDF
46. Isolation of Helicobacter pylori from the intestinal mucosa of patients with Crohn's disease.
- Author
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Oliveira AG, Rocha GA, Rocha AM, Sanna Md, Moura SB, Dani R, Marinho FP, Moreira LS, Ferrari Mde L, Castro LP, and Queiroz DM
- Subjects
- Adolescent, Adult, Aged, Case-Control Studies, Crohn Disease etiology, DNA, Bacterial analysis, Female, Helicobacter Infections microbiology, Helicobacter pylori genetics, Humans, Immunoglobulin G analysis, Male, Middle Aged, Polymerase Chain Reaction methods, RNA, Ribosomal, 16S genetics, Crohn Disease microbiology, Helicobacter pylori isolation & purification, Intestinal Mucosa microbiology
- Abstract
Background: Helicobacter species are associated with inflammatory bowel disease in rodents and in nonhuman primates. Therefore, we prospectively investigated the presence of Helicobacter species in the intestinal mucosa of patients with and without Crohn's disease by culture and polymerase chain reaction (PCR) assays., Materials and Methods: Mucosal fragments were obtained from the ileum, different colon regions, and rectum of 43 patients with Crohn's disease and of 74 patients without inflammatory bowel disease., Results: Helicobacter pylori strains, identified by 16S rRNA gene sequencing, were more frequently isolated and PCR-detected in the intestinal mucosa of patients with ulcerative colitis-like Crohn's disease than in intestinal mucosa of the control group. Otherwise, anti-H. pylori immunoglobulin G levels were significantly lower in fibrostenosing and fistulating Crohn's disease subgroups. No other Helicobacter species were found in the intestinal mucosa of the patients., Conclusions: Although our results suggest an association between the presence of H. pylori in the intestine and ulcerative colitis-like phenotype of Crohn's disease, H. pylori infection in the actual causality of Crohn's disease is still to be determined.
- Published
- 2006
- Full Text
- View/download PDF
47. Helicobacter species in the intestinal mucosa of patients with ulcerative colitis.
- Author
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Oliveira AG, das Graças Pimenta Sanna M, Rocha GA, Rocha AM, Santos A, Dani R, Marinho FP, Moreira LS, de Lourdes Abreu Ferrari M, Moura SB, Castro LP, and Queiroz DM
- Subjects
- Adolescent, Adult, Aged, Female, Humans, Male, Middle Aged, Colitis, Ulcerative microbiology, Helicobacter isolation & purification, Intestinal Mucosa microbiology
- Abstract
In a search for Helicobacter species in the intestinal mucosae of 42 patients with ulcerative colitis (UC) and 74 without UC, only H. pylori was found. Although the bacterium was detected in UC patients by culture (7.1%) and nested PCR (19.0%), its presence was not associated with the disease (P = 0.13).
- Published
- 2004
- Full Text
- View/download PDF
48. Clinical evaluation of patients with epidermolysis bullosa: review of the literature and case reports.
- Author
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Silva LC, Cruz RA, Abou-Id LR, Brini LN, and Moreira LS
- Subjects
- Anemia etiology, Child, Dental Caries etiology, Dental Caries therapy, Female, Humans, Male, Microstomia etiology, Tongue abnormalities, Dental Care for Chronically Ill, Epidermolysis Bullosa complications
- Abstract
Epidermolysis bullosa (EB) is a relatively rare inherited disorder, which includes blister and vesicle formation on the skin and mucous membranes as a result of trauma or heat. There are different forms of this disorder. Mild manifestations are relatively uncomfortable, usually involving the knees, elbows, and fingers. Severe forms of this disease compromise normal functioning of multiple organs, which may result in premature death. The lack of a specific treatment to cure EB makes genetic counseling and prenatal diagnosis of primary importance to control this disorder. Three case histories of persons with dystrophic recessive epidermolysis bullosa are reported, focusing on appropriate dental care for patients with EB.
- Published
- 2004
- Full Text
- View/download PDF
49. Factors associated with treatment failure of Helicobacter pylori infection in a developing country.
- Author
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Queiroz DM, Dani R, Silva LD, Santos A, Moreira LS, Rocha GA, Corrêa PR, Reis LF, Nogueira AM, Alvares Cabral MM, Esteves AM, and Tanure J
- Subjects
- 2-Pyridinylmethylsulfinylbenzimidazoles, Adolescent, Adult, Aged, Anti-Bacterial Agents administration & dosage, Anti-Infective Agents, Local administration & dosage, Anti-Ulcer Agents administration & dosage, Benzimidazoles administration & dosage, Clarithromycin administration & dosage, Drug Resistance, Bacterial, Drug Therapy, Combination, Female, Furazolidone administration & dosage, Humans, Male, Middle Aged, Omeprazole analogs & derivatives, Pantoprazole, Risk Factors, Sex Factors, Sulfoxides administration & dosage, Anti-Bacterial Agents therapeutic use, Anti-Infective Agents, Local therapeutic use, Anti-Ulcer Agents therapeutic use, Benzimidazoles therapeutic use, Clarithromycin therapeutic use, Developing Countries, Furazolidone therapeutic use, Helicobacter Infections drug therapy, Helicobacter pylori drug effects, Sulfoxides therapeutic use, Treatment Failure
- Abstract
Background: Although no regimen can eradicate in 100% of patients, factors that may affect the eradication rates have been poorly studied., Goal: To evaluate factors associated with treatment failure., Study: One hundred patients were treated with pantoprazole plus clarithromycin and furazolidone for eradication. Clarithromycin and furazolidone resistance was evaluated by the agar dilution method. Point mutations in 23S rRNA genes related to clarithromycin resistance were investigated by polymerase chain reaction and restriction length fragment polymorphism and A by polymerase chain reaction. The data were analyzed by logistic regression., Results: eradication occurred in 85 of 97 patients who completed the treatment (87.6%; 95% CI = 79.0-93.1). All strains were susceptible to furazolidone, and nine were resistant to clarithromycin (A2142G or A2143G mutation was detected in all of them). The treatment failure was significant and independently associated with clarithromycin resistance (OR = 7.79; 95% CI = 1.73-35.01), A-negative status (OR = 4.81; 95% CI = 1.14-20.14), and male gender (OR = 4.20; 95% CI = 1.01-17.78), but not with the type of disease, mean age, smoking, alcohol consumption, and the degree of the antral and oxyntic gastritis., Conclusion: Resistance to clarithromycin, A-negative status, and gender were predictive factors of eradication failure.
- Published
- 2002
- Full Text
- View/download PDF
50. Omeprazole, clarithromycin and furazolidone for the eradication of Helicobacter pylori in patients with duodenal ulcer.
- Author
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Dani R, Queiroz DM, Dias MG, Franco JM, Magalhães LC, Mendes GS, Moreira LS, De Castro LP, Toppa NH, Rocha GA, Cabral MM, and Salles PG
- Subjects
- Adolescent, Adult, Anti-Bacterial Agents administration & dosage, Anti-Bacterial Agents adverse effects, Anti-Ulcer Agents administration & dosage, Anti-Ulcer Agents adverse effects, Brazil, Clarithromycin adverse effects, Drug Therapy, Combination, Duodenal Ulcer pathology, Endoscopy, Female, Furazolidone adverse effects, Humans, Male, Middle Aged, Omeprazole adverse effects, Random Allocation, Time Factors, Clarithromycin administration & dosage, Duodenal Ulcer drug therapy, Furazolidone administration & dosage, Helicobacter Infections drug therapy, Helicobacter pylori, Omeprazole administration & dosage
- Abstract
Aim: To evaluate the efficacy of omeprazole plus clarithromycin and furazolidone in Helicobacter pylori eradication and duodenal ulcer healing in Brazilian patients., Methods: Forty H. pylori-positive patients with duodenal ulcer were randomized to receive 20 mg omeprazole o.m. or b.d. for 1 month plus 500 mg clarithromycin (b.d. ) and 200 mg furazolidone (b.d.) for 1 week., Results: Three months after the end of the treatment the eradication rates were 90% by intention-to-treat analysis, and 97% by per protocol analysis. Mild side-effects were observed in 25 patients, none of whom abandoned the protocol. No difference was observed between the 20 mg and 40 mg omeprazole daily doses. Cure or significant improvement of the symptoms and of the histological alterations were observed after H. pylori eradication., Conclusion: Our results demonstrate that clarithromycin and furazolidone in combination with omeprazole are a good alternative for H. pylori eradication in Brazilian patients with duodenal ulcer.
- Published
- 1999
- Full Text
- View/download PDF
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