1. Gastroprotective potential of frutalin, a D-galactose binding lectin, against ethanol-induced gastric lesions.
- Author
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de Vasconcellos Abdon AP, Coelho de Souza G, Noronha Coelho de Souza L, Prado Vasconcelos R, Araújo Castro C, Moreira Guedes M, Pereira Lima RC Jr, de Azevedo Moreira R, de Oliveira Monteiro-Moreira AC, and Rolim Campos A
- Subjects
- Animals, Anti-Ulcer Agents pharmacology, Antioxidants metabolism, Antioxidants pharmacology, Biomarkers metabolism, Capsaicin analogs & derivatives, Capsaicin pharmacology, Cimetidine therapeutic use, Ethanol, Galactose metabolism, Galectins pharmacology, Gastric Mucosa pathology, Glutathione metabolism, Glyburide pharmacology, Indomethacin pharmacology, Male, Malondialdehyde metabolism, Mice, Mice, Inbred Strains, NG-Nitroarginine Methyl Ester pharmacology, Oxidative Stress drug effects, Plant Extracts pharmacology, Stomach Ulcer chemically induced, Stomach Ulcer metabolism, Anti-Ulcer Agents therapeutic use, Antioxidants therapeutic use, Galectins therapeutic use, Gastric Mucosa drug effects, Phytotherapy, Plant Extracts therapeutic use, Stomach Ulcer drug therapy
- Abstract
The present study was designed to verify whether frutalin (FTL) affords gastroprotection against the ethanol-induced gastric damage and to examine the underlying mechanism(s). Gastric damage was induced by intragastric administration of 0.2 ml of ethanol (96%). Mice in groups were pretreated with FTL (0.25, 0.5 and 1 mg/kg; i.p.), cimetidine (100 mg/kg; p.o.), or vehicle (0.9% of NaCl, 10 mL/kg; p.o.), 30 min before ethanol administration. They were sacrificed 30 min later, the stomachs excised, and the mucosal lesion area (mm²) measured by planimetry. Gastroprotection was assessed in relation to inhibition of gastric lesion area. To study the gastroprotective mechanism(s), its relations to capsaicin-sensitive fibers, endogenous prostaglandins, nitric oxide, sulphydryls, ATP-sensitive potassium channels, adrenoceptors, opioid receptors and calcium channels were analyzed. Treatments effects on ethanol-associated oxidative stress markers GSH and MDA were measured in gastric tissue. FTL afforded a dose-unrelated gastroprotection against the ethanol damage. However, it failed to prevent the ethanol-induced changes in the levels of GSH and MDA. It was observed that the gastroprotection by FTL was greatly reduced in animals pretreated with capsazepine, indomethacin, L-NAME or glibenclamide. Considering the results, it is suggested that the FTL could probably be a good therapeutic agent for the development of new medicine for the treatment of gastric ulcer., (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Published
- 2012
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