Search

Your search keyword '"Morasso, Maria I"' showing total 318 results
Start Over Author "Morasso, Maria I"
318 results on '"Morasso, Maria I"'

4. Molecular mechanisms regulating wound repair: Evidence for paracrine signaling from corneal epithelial cells to fibroblasts and immune cells following transient epithelial cell treatment with Mitomycin C

Author

Pal-Ghosh, Sonali, Karpinski, Beverly A., Datta Majumdar, Himani, Ghosh, Trisha, Thomasian, Julie, Brooks, Stephen R., Sawaya, Andrew P., Morasso, Maria I., Scholand, Kaitlin K., de Paiva, Cintia S., Galletti, Jeremias G., and Stepp, Mary Ann

Published
2023
Full Text
View/download PDF

5. Transcriptional signature primes human oral mucosa for rapid wound healing

Author

Iglesias-Bartolome, Ramiro, Uchiyama, Akihiko, Molinolo, Alfredo A, Abusleme, Loreto, Brooks, Stephen R, Callejas-Valera, Juan Luis, Edwards, Dean, Doci, Colleen, Asselin-Labat, Marie-Liesse, Onaitis, Mark W, Moutsopoulos, Niki M, Gutkind, J Silvio, and Morasso, Maria I

Subjects
Medical Biotechnology, Biomedical and Clinical Sciences, Wound Healing and Care, Dental/Oral and Craniofacial Disease, Genetics, 1.1 Normal biological development and functioning, 2.1 Biological and endogenous factors, Inflammatory and immune system, Skin, Biopsy, Humans, Keratinocytes, Mouth Mucosa, Transcription Factors, Wound Healing, Biological Sciences, Medical and Health Sciences, Medical biotechnology, Biomedical engineering
Abstract

Oral mucosal wound healing has long been regarded as an ideal system of wound resolution. However, the intrinsic characteristics that mediate optimal healing at mucosal surfaces are poorly understood, particularly in humans. We present a unique comparative analysis between human oral and cutaneous wound healing using paired and sequential biopsies during the repair process. Using molecular profiling, we determined that wound-activated transcriptional networks are present at basal state in the oral mucosa, priming the epithelium for wound repair. We show that oral mucosal wound-related networks control epithelial cell differentiation and regulate inflammatory responses, highlighting fundamental global mechanisms of repair and inflammatory responses in humans. The paired comparative analysis allowed for the identification of differentially expressed SOX2 (sex-determining region Y-box 2) and PITX1 (paired-like homeodomain 1) transcriptional regulators in oral versus skin keratinocytes, conferring a unique identity to oral keratinocytes. We show that SOX2 and PITX1 transcriptional function has the potential to reprogram skin keratinocytes to increase cell migration and improve wound resolution in vivo. Our data provide insights into therapeutic targeting of chronic and nonhealing wounds based on greater understanding of the biology of healing in human mucosal and cutaneous environments.

Published
2018

6. A data mining paradigm for identifying key factors in biological processes using gene expression data.

Author

Li, Jin, Zheng, Le, Uchiyama, Akihiko, Bin, Lianghua, Mauro, Theodora M, Elias, Peter M, Pawelczyk, Tadeusz, Sakowicz-Burkiewicz, Monika, Trzeciak, Magdalena, Leung, Donald YM, Morasso, Maria I, and Yu, Peng

Subjects
Skin, Animals, Humans, Mice, Cluster Analysis, Gene Expression Profiling, Biological Phenomena, Thermogenesis, Cold Temperature, Data Mining, Gene Ontology
Abstract

A large volume of biological data is being generated for studying mechanisms of various biological processes. These precious data enable large-scale computational analyses to gain biological insights. However, it remains a challenge to mine the data efficiently for knowledge discovery. The heterogeneity of these data makes it difficult to consistently integrate them, slowing down the process of biological discovery. We introduce a data processing paradigm to identify key factors in biological processes via systematic collection of gene expression datasets, primary analysis of data, and evaluation of consistent signals. To demonstrate its effectiveness, our paradigm was applied to epidermal development and identified many genes that play a potential role in this process. Besides the known epidermal development genes, a substantial proportion of the identified genes are still not supported by gain- or loss-of-function studies, yielding many novel genes for future studies. Among them, we selected a top gene for loss-of-function experimental validation and confirmed its function in epidermal differentiation, proving the ability of this paradigm to identify new factors in biological processes. In addition, this paradigm revealed many key genes in cold-induced thermogenesis using data from cold-challenged tissues, demonstrating its generalizability. This paradigm can lead to fruitful results for studying molecular mechanisms in an era of explosive accumulation of publicly available biological data.

Published
2018

31. Hair keratin mutations in tooth enamel increase dental decay risk

Author

Duverger, Olivier, Ohara, Takahiro, Shaffer, John R., Donahue, Danielle, Zerfas, Patricia, Dullnig, Andrew, Crecelius, Christopher, Beniash, Elia, Marazita, Mary L., and Morasso, Maria I.

Subjects
Gene mutations -- Health aspects -- Research, Keratin -- Genetic aspects -- Research, Dental caries -- Genetic aspects -- Risk factors -- Research, Health care industry
Abstract

Tooth enamel is the hardest substance in the human body and has a unique combination of hardness and fracture toughness that protects teeth from dental caries, the most common chronic disease worldwide. In addition to a high mineral content, tooth enamel comprises organic material that is important for mechanical performance and influences the initiation and progression of caries; however, the protein composition of tooth enamel has not been fully characterized. Here, we determined that epithelial hair keratins, which are crucial for maintaining the integrity of the sheaths that support the hair shaft, are expressed in the enamel organ and are essential organic components of mature enamel. Using genetic and intraoral examination data from 386 children and 706 adults, we found that individuals harboring known hair disorder-associated polymorphisms in the gene encoding keratin 75 (KRT75), [KRT75.sup.A161T] and [KRT75.sup.E337K], are prone to increased dental caries. Analysis of teeth from individuals carrying the [KRT75.sup.A161T] variant revealed an altered enamel structure and a marked reduction of enamel hardness, suggesting that a functional keratin network is required for the mechanical stability of tooth enamel. Taken together, our results identify a genetic locus that influences enamel structure and establish a connection between hair disorders and susceptibility to dental caries., Introduction Dental caries are initiated at the surface of the tooth by microorganisms metabolizing food residues and releasing acids that dissolve enamel minerals (1). Their development is influenced by nutrition, [...]

Published
2014
Full Text
View/download PDF

38. Interferon lambda promotes immune dysregulation and tissue inflammation in TLR7-induced lupus

Author

Wang, Xinghao, primary, Goel, Rishi R., additional, O’Neil, Liam J., additional, Nakabo, Shuichiro, additional, Hasneen, Kowser, additional, Gupta, Sarthak, additional, Wigerblad, Gustaf, additional, Blanco, Luz P., additional, Kopp, Jeffrey B., additional, Morasso, Maria I., additional, Kotenko, Sergei V., additional, Yu, Zu-Xi, additional, Carmona-Rivera, Carmelo, additional, and Kaplan, Mariana J., additional

Published
2020
Full Text
View/download PDF

41. The homeodomain gene Xenopus Distal-less-like-2 (Xdll-2) is regulated by a conserved mechanism in amphibian and mammalian epidermis

Author

Morasso, Maria I., Jamrich, Milan, and Sargent, Thomas D.

Subjects
Xenopus -- Genetic aspects, Epidermis -- Research, Frogs -- Anatomy, Biological sciences
Abstract

An analysis of the expression of frog embryo epidermis of Xenopus distal-less-like-2 (Xdll-2), a gene encoding a homeodomain protein, confirms that a regulatory 5' flanking DNA site situated inside 933 bp at the onset of transcription specifies this expression. Induction dependent repression in frog ectoderm cells treated in vitro with activin A and calcium-inducible expression in cultured murine keratinocytes results for a globin reporter gene, in consequence to the action of this regulatory DNA.

Published
1994

47. The skin transcriptome in hidradenitis suppurativa uncovers an antimicrobial and sweat gland gene signature which has distinct overlap with wounded skin

Author

Coates, Margaret, primary, Mariottoni, Paula, additional, Corcoran, David L., additional, Kirshner, Hélène Fradin, additional, Jaleel, Tarannum, additional, Brown, David A., additional, Brooks, Stephen R., additional, Murray, John, additional, Morasso, Maria I., additional, and MacLeod, Amanda S., additional

Published
2019
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results