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Introduction. This article emphasizes the importance of the informed and careful practice in pediatric dentistry and endodontics to prevent iatrogenic events. It highlights critical need to select appropriate obturation materials, especially in deciduous teeth, and the use of diagnostic tools such as panoramic radiographs and computed tomography scans, to avoid complications. Case report. The case of a pediatric patient of 8 years and one month old is presented, with a pulpectomy in the OD 53 obturated with gutta-percha, which hindered the eruption of teeth 13 and 14. This case is rare due to the unusual difficulty in eruption caused by gutta-percha obturation in a deciduous tooth, not commonly reported in literature. Conclusions. Meticulous dental practice supported by clear protocols is essential to ensure successful and safe treatment in pediatric patients. Proper selection of obturation materials, particularly in deciduous dentition, is crucial. The findings underscore the relevance of diagnostic tools such as panoramic radiography and computed tomography. Specialized attention and the use of resorbable materials in deciduous dentition are critical to prevent future complications. [ABSTRACT FROM AUTHOR]

The diagnosis of cardiovascular disease (CVD) is still limited. Therefore, this study demonstrates the presence of human ether-a-go-go-related gene 1 (hERG1) and heat shock protein 47 (Hsp47) on the surface of small extracellular vesicles (sEVs) in human peripheral blood and their association with CVD. In this research, 20 individuals with heart failure and 26 participants subjected to cardiac stress tests were enrolled. The associations between hERG1 and/or Hsp47 in sEVs and CVD were established using Western blot, flow cytometry, electron microscopy, ELISA, and nanoparticle tracking analysis. The results show that hERG1 and Hsp47 were present in sEV membranes, extravesicularly exposing the sequences 430AFLLKETEEGPPATE445 for hERG1 and 169ALQSINEWAAQTT- DGKLPEVTKDVERTD196 for Hsp47. In addition, upon exposure to hypoxia, rat primary cardiomyocytes released sEVs into the media, and human cardiomyocytes in culture also released sEVs containing hERG1 (EV-hERG1) and/or Hsp47 (EV-Hsp47). Moreover, the levels of sEVs increased in the blood when cardiac ischemia was induced during the stress test, as well as the concentrations of EV-hERG1 and EV-Hsp47. Additionally, the plasma levels of EV-hERG1 and EV-Hsp47 decreased in patients with decompensated heart failure (DHF). Our data provide the first evidence that hERG1 and Hsp47 are present in the membranes of sEVs derived from the human cardiomyocyte cell line, and also in those isolated from human peripheral blood. Total sEVs, EV-hERG1, and EV-Hsp47 may be explored as biomarkers for heart diseases such as heart failure and cardiac ischemia. [ABSTRACT FROM AUTHOR]

Objective: To determine adolescent satisfaction from the differentiated health care service at a primary health care institution in Lima. Materials and methods: An observational, descriptive and cross-sectional study conducted in 84 adolescents treated at the Centro de Salud El Progreso (Carabayllo). Satisfaction from health care was evaluated using the SERVQUAL questionnaire, which was adapted and validated, and showed reliability in its expectation and perception components. Results: Satisfaction from health care occurred in 28.57 % of the adolescents, most of whom were between 12 and 14 years old (83.33 %). Regarding the dimensions, empathy and security had the highest percentage of satisfaction (39.28 % and 36.90 %, respectively). The indicators physical appearance of the facilities (6.45 ± 0.82) and trust in health care (6.38 ± 0.84) presented the highest score in expectations. Conclusions: A small proportion of adolescents who attended the differentiated service were satisfied with the care received, while the empathy dimension was the one with the greatest number of adolescents who showed satisfaction. [ABSTRACT FROM AUTHOR]

The activity of L-type calcium channels is associated with the duration of the plateau phase of the cardiac action potential (AP) and it is controlled by voltage- and calcium-dependent inactivation (VDI and CDI, respectively). During β-adrenergic stimulation, an increase in the L-type current and parallel changes in VDI and CDI are observed during square pulses stimulation; however, how these modifications impact calcium currents during an AP remains controversial. Here, we examined the role of both inactivation processes on the L-type calcium current activity in newborn rat cardiomyocytes in control conditions and after stimulation with the β-adrenergic agonist isoproterenol. Our approach combines a self-AP clamp (sAP-Clamp) with the independent inhibition of VDI or CDI (by overexpressing CaVβ2a or calmodulin mutants, respectively) to directly record the L-type calcium current during the cardiac AP. We find that at room temperature (20-23°C) and in the absence of β-adrenergic stimulation, the L-type current recapitulates the AP kinetics. Furthermore, under our experimental setting, the activity of the sodium-calcium exchanger (NCX) does not affect the shape of the AP. We find that hindering either VDI or CDI prolongs the L-type current and the AP in parallel, suggesting that both inactivation processes modulate the L-type current during the AP. In the presence of isoproterenol, wild-type and VDI-inhibited cardiomyocytes display mismatched L-type calcium current with respect to their AP. In contrast, CDI-impaired cells maintain L-type current with kinetics similar to its AP, demonstrating that calcium-dependent inactivation governs L-type current kinetics during β-adrenergic stimulation. [ABSTRACT FROM AUTHOR]

The cardiac L-type calcium channel is a multi-subunit complex that requires co-assembling of the pore-forming subunit Ca V 1.2 with auxiliary subunits Ca V α 2 δ and Ca V β. Its traffic has been shown to be controlled by these subunits and by the activation of various G-protein coupled receptors (GPCR). Here, we explore the consequences of the prolonged activation of angiotensin receptor type 1 (AT 1 R) over Ca V 1.2 channel trafficking. Bioluminescence Resonance Energy Transfer (BRET) assay between β-arrestin and L-type channels in angiotensin II-stimulated cells was used to assess the functional consequence of AT 1 R activation, while immunofluorescence of adult rat cardiomyocytes revealed the effects of GPCR activation on Ca V 1.2 trafficking. Angiotensin II exposure results in β-arrestin 1 recruitment to the channel complex and an apparent loss of Ca V 1.2 immunostaining at the T-tubules. Accordingly, angiotensin II stimulation causes a decrease in L-type current, Ca 2+ transients and myocyte contractility, together with a faster repolarization phase of action potentials. Our results demonstrate that prolonged AT 1 R activation induces β-arrestin 1 recruitment and the subsequent internalization of Ca V 1.2 channels with a half-dose of AngII on the order of 100 nM, suggesting that this effect depends on local renin-angiotensin system. This novel AT 1 R-dependent Ca V 1.2-trafficking modulation likely contributes to angiotensin II-mediated cardiac remodeling.