25 results on '"Monné Rodríguez, Josep M."'
Search Results
2. Preclinical evaluation of 5-methyltetrahydrofolate-based radioconjugates—new perspectives for folate receptor–targeted radionuclide therapy
- Author
-
Guzik, Patrycja, Benešová, Martina, Ratz, Magdalena, Monné Rodríguez, Josep M., Deberle, Luisa M., Schibli, Roger, and Müller, Cristina
- Published
- 2021
- Full Text
- View/download PDF
3. Microengineered biosynthesized cellulose as anti-fibrotic in vivo protection for cardiac implantable electronic devices
- Author
-
Robotti, Francesco, Sterner, Ita, Bottan, Simone, Monné Rodríguez, Josep M., Pellegrini, Giovanni, Schmidt, Tanja, Falk, Volkmar, Poulikakos, Dimos, Ferrari, Aldo, and Starck, Christoph
- Published
- 2020
- Full Text
- View/download PDF
4. European Society of Toxicologic Pathology (Pathology 2.0 Molecular Pathology Special Interest Group): Review of In Situ Hybridization Techniques for Drug Research and Development
- Author
-
Monné Rodríguez, Josep M., primary, Frisk, Anna-Lena, additional, Kreutzer, Robert, additional, Lemarchand, Thomas, additional, Lezmi, Stephane, additional, Saravanan, Chandrassegar, additional, Stierstorfer, Birgit, additional, Thuilliez, Céline, additional, Vezzali, Enrico, additional, Wieczorek, Grazyna, additional, Yun, Seong-Wook, additional, and Schaudien, Dirk, additional
- Published
- 2023
- Full Text
- View/download PDF
5. Tissue Glue-Based Sealing Patch for the in vivo Prevention of Iatrogenic Prelabor Preterm Rupture of Fetal Membranes
- Author
-
Devaud, Yannick Robert, Avilla-Royo, Eva; https://orcid.org/0000-0002-1002-8491, Lionetti, Leonardo, Tronnier, Helena, Seehusen, Frauke; https://orcid.org/0000-0001-6701-7868, Monné Rodríguez, Josep M; https://orcid.org/0000-0002-2574-0780, Moehrlen, Ueli; https://orcid.org/0000-0001-6418-1136, Weisskopf, Miriam; https://orcid.org/0000-0001-6012-3051, Vonzun, Ladina; https://orcid.org/0000-0003-1301-8608, Strübing, Nele; https://orcid.org/0000-0003-0676-0329, Ochsenbein-Kölble, Nicole; https://orcid.org/0000-0002-7691-965X, Ehrbar, Martin; https://orcid.org/0000-0003-2707-4870, Devaud, Yannick Robert, Avilla-Royo, Eva; https://orcid.org/0000-0002-1002-8491, Lionetti, Leonardo, Tronnier, Helena, Seehusen, Frauke; https://orcid.org/0000-0001-6701-7868, Monné Rodríguez, Josep M; https://orcid.org/0000-0002-2574-0780, Moehrlen, Ueli; https://orcid.org/0000-0001-6418-1136, Weisskopf, Miriam; https://orcid.org/0000-0001-6012-3051, Vonzun, Ladina; https://orcid.org/0000-0003-1301-8608, Strübing, Nele; https://orcid.org/0000-0003-0676-0329, Ochsenbein-Kölble, Nicole; https://orcid.org/0000-0002-7691-965X, and Ehrbar, Martin; https://orcid.org/0000-0003-2707-4870
- Abstract
Introduction: One of the main concerns for all fetal surgeries is the risk of preterm delivery due to the preterm prelabor rupture of the fetal membranes (iPPROM). Clinical approaches to seal fetal membrane (FM) defects are missing due to the lack of appropriate strategies to apply sealing biomaterials at the defect site. Methods: Here, we test the performance of a previously developed strategy to seal FM defects with cyanoacrylate-based sealing patches in an ovine model up to 24 days after application. Results: Patches sealed tightly the fetoscopy-induced FM defects and remained firmly attached to the defect over 10 days. At 10 days after treatment, 100% (13/13) of the patches were attached to the FMs, and 24 days after treatment 25% (1/4) of the patches placed in CO$_2$ insufflation, and 33% (1/3) in NaCl infusion remained. However, all successfully applied patches (20/24) led to a watertight sealing at 10 or 24 days after treatment. Histological analysis indicated that cyanoacrylates induced a moderate immune response and disrupted the FM epithelium. Conclusion: Together, these data show the feasibility of minimally invasive sealing of FM defects by locally gathering tissue adhesive. Further development to combine this technology with refined tissue glues or healing-inducing materials holds great promise for future clinical translation.
- Published
- 2023
6. European Society of Toxicologic Pathology (Pathology 2.0 Molecular Pathology Special Interest Group): Review of In Situ Hybridization Techniques for Drug Research and Development
- Author
-
Monné Rodríguez, Josep M; https://orcid.org/0000-0002-2574-0780, Frisk, Anna-Lena; https://orcid.org/0000-0002-3695-1997, Kreutzer, Robert, Lemarchand, Thomas, Lezmi, Stephane, Saravanan, Chandrassegar; https://orcid.org/0000-0003-3214-5696, Stierstorfer, Birgit, Thuilliez, Céline, Vezzali, Enrico; https://orcid.org/0000-0002-5550-9387, Wieczorek, Grazyna, Yun, Seong-Wook, Schaudien, Dirk; https://orcid.org/0000-0001-7398-7262, Monné Rodríguez, Josep M; https://orcid.org/0000-0002-2574-0780, Frisk, Anna-Lena; https://orcid.org/0000-0002-3695-1997, Kreutzer, Robert, Lemarchand, Thomas, Lezmi, Stephane, Saravanan, Chandrassegar; https://orcid.org/0000-0003-3214-5696, Stierstorfer, Birgit, Thuilliez, Céline, Vezzali, Enrico; https://orcid.org/0000-0002-5550-9387, Wieczorek, Grazyna, Yun, Seong-Wook, and Schaudien, Dirk; https://orcid.org/0000-0001-7398-7262
- Abstract
In situ hybridization (ISH) is used for the localization of specific nucleic acid sequences in cells or tissues by complementary binding of a nucleotide probe to a specific target nucleic acid sequence. In the last years, the specificity and sensitivity of ISH assays were improved by innovative techniques like synthetic nucleic acids and tandem oligonucleotide probes combined with signal amplification methods like branched DNA, hybridization chain reaction and tyramide signal amplification. These improvements increased the application spectrum for ISH on formalin-fixed paraffin-embedded tissues. ISH is a powerful tool to investigate DNA, mRNA transcripts, regulatory noncoding RNA, and therapeutic oligonucleotides. ISH can be used to obtain spatial information of a cell type, subcellular localization, or expression levels of targets. Since immunohistochemistry and ISH share similar workflows, their combination can address simultaneous transcriptomics and proteomics questions. The goal of this review paper is to revisit the current state of the scientific approaches in ISH and its application in drug research and development.
- Published
- 2023
7. sj-docx-1-spp-10.1177_01926233231178282 – Supplemental material for European Society of Toxicologic Pathology (Pathology 2.0 Molecular Pathology Special Interest Group): Review of In Situ Hybridization Techniques for Drug Research and Development
- Author
-
Monné Rodríguez, Josep M., Frisk, Anna-Lena, Kreutzer, Robert, Lemarchand, Thomas, Lezmi, Stephane, Saravanan, Chandrassegar, Stierstorfer, Birgit, Thuilliez, Céline, Vezzali, Enrico, Wieczorek, Grazyna, Yun, Seong-Wook, and Schaudien, Dirk
- Subjects
Pharmacology and Pharmaceutical Sciences not elsewhere classified - Abstract
Supplemental material, sj-docx-1-spp-10.1177_01926233231178282 for European Society of Toxicologic Pathology (Pathology 2.0 Molecular Pathology Special Interest Group): Review of In Situ Hybridization Techniques for Drug Research and Development by Josep M. Monné Rodríguez, Anna-Lena Frisk, Robert Kreutzer, Thomas Lemarchand, Stephane Lezmi, Chandrassegar Saravanan, Birgit Stierstorfer, Céline Thuilliez, Enrico Vezzali, Grazyna Wieczorek, Seong-Wook Yun and Dirk Schaudien in Toxicologic Pathology
- Published
- 2023
- Full Text
- View/download PDF
8. Vaccination-based immunotherapy to target profibrotic cells in liver and lung
- Author
-
Sobecki, Michal; https://orcid.org/0000-0002-8177-0327, Chen, Jing M; https://orcid.org/0000-0002-2143-6984, Krzywinska, Ewelina; https://orcid.org/0000-0002-4311-2972, Nagarajan, Shunmugam; https://orcid.org/0000-0001-6676-954X, Fan, Zheng, Nelius, Eric, Monné Rodríguez, Josep M; https://orcid.org/0000-0002-2574-0780, Seehusen, Frauke; https://orcid.org/0000-0001-6701-7868, Hussein, Amro, Moschini, Greta, Hajam, Edries Y, Kiran, Ravi, Gotthardt, Dagmar; https://orcid.org/0000-0002-8416-1236, Debbache, Julien, Badoual, Cécile, Sato, Tatsuyuki; https://orcid.org/0000-0002-9229-9016, Isagawa, Takayuki, Takeda, Norihiko, Tanchot, Corinne, Tartour, Eric, Weber, Achim; https://orcid.org/0000-0003-0073-3637, Werner, Sabine; https://orcid.org/0000-0001-7397-8710, Loffing, Johannes; https://orcid.org/0000-0002-9415-6588, Sommer, Lukas; https://orcid.org/0000-0002-1143-7908, Sexl, Veronika; https://orcid.org/0000-0001-9363-0412, Münz, Christian; https://orcid.org/0000-0001-6419-1940, Feghali-Bostwick, Carol, Pachera, Elena, Distler, Oliver; https://orcid.org/0000-0002-0546-8310, Sobecki, Michal; https://orcid.org/0000-0002-8177-0327, Chen, Jing M; https://orcid.org/0000-0002-2143-6984, Krzywinska, Ewelina; https://orcid.org/0000-0002-4311-2972, Nagarajan, Shunmugam; https://orcid.org/0000-0001-6676-954X, Fan, Zheng, Nelius, Eric, Monné Rodríguez, Josep M; https://orcid.org/0000-0002-2574-0780, Seehusen, Frauke; https://orcid.org/0000-0001-6701-7868, Hussein, Amro, Moschini, Greta, Hajam, Edries Y, Kiran, Ravi, Gotthardt, Dagmar; https://orcid.org/0000-0002-8416-1236, Debbache, Julien, Badoual, Cécile, Sato, Tatsuyuki; https://orcid.org/0000-0002-9229-9016, Isagawa, Takayuki, Takeda, Norihiko, Tanchot, Corinne, Tartour, Eric, Weber, Achim; https://orcid.org/0000-0003-0073-3637, Werner, Sabine; https://orcid.org/0000-0001-7397-8710, Loffing, Johannes; https://orcid.org/0000-0002-9415-6588, Sommer, Lukas; https://orcid.org/0000-0002-1143-7908, Sexl, Veronika; https://orcid.org/0000-0001-9363-0412, Münz, Christian; https://orcid.org/0000-0001-6419-1940, Feghali-Bostwick, Carol, Pachera, Elena, and Distler, Oliver; https://orcid.org/0000-0002-0546-8310
- Abstract
Fibrosis is the final path of nearly every form of chronic disease, regardless of the pathogenesis. Upon chronic injury, activated, fibrogenic fibroblasts deposit excess extracellular matrix, and severe tissue fibrosis can occur in virtually any organ. However, antifibrotic therapies that target fibrogenic cells, while sparing homeostatic fibroblasts in healthy tissues, are limited. We tested whether specific immunization against endogenous proteins, strongly expressed in fibrogenic cells but highly restricted in quiescent fibroblasts, can elicit an antigen-specific cytotoxic T cell response to ameliorate organ fibrosis. In silico epitope prediction revealed that activation of the genes Adam12 and Gli1 in profibrotic cells and the resulting “self-peptides” can be exploited for T cell vaccines to ablate fibrogenic cells. We demonstrate the efficacy of a vaccination approach to mount CD8+ T cell responses that reduce fibroblasts and fibrosis in the liver and lungs in mice. These results provide proof of principle for vaccination-based immunotherapies to treat fibrosis.
- Published
- 2022
9. Influence of High Energy Diet and Polygenic Predisposition for Obesity on Postpartum Health in Rat Dams
- Author
-
Leuthardt, Andrea Stephanie, Bayer, Julia, Monné Rodríguez, Josep M, Boyle, Christina N; https://orcid.org/0000-0002-1060-2529, Leuthardt, Andrea Stephanie, Bayer, Julia, Monné Rodríguez, Josep M, and Boyle, Christina N; https://orcid.org/0000-0002-1060-2529
- Abstract
It is estimated that 30% of pregnant women worldwide are overweight or obese, leading to adverse health effects for both mother and child. Women with obesity during pregnancy are at higher risk for developing both metabolic and mental disorders, such as diabetes and depression. Numerous studies have used rodent models of maternal obesity to understand its consequences on the offspring, yet characterization of changes in the dams is rare, and most rodent models rely solely on a high fat diet to induce maternal obesity, without regarding genetic propensity for obesity. Here we present the influence of both peripartum high energy diet (HE) and obesity-proneness on maternal health using selectively bred diet-resistant (DR) and diet-induced obese (DIO) rat dams. Outbred Sprague-Dawley rats were challenged with HE diet prior to mating and bred according to their propensity to gain weight. The original outbred breeding dams (F0) were maintained on low-fat chow during pregnancy and lactation. By comparison, the F1 dams consuming HE diet during pregnancy and lactation displayed higher gestational body weight gain (P < 0.01), and HE diet caused increased meal size and reduced meal frequency (P < 0.001). Sensitivity to the hormone amylin was preserved during pregnancy, regardless of diet. After several rounds of selective breeding, DIO and DR dams from generation F3 were provided chow or HE during pregnancy and lactation and assessed for their postpartum physiology and behaviors. We observed strong diet and phenotype effects on gestational weight gain, with DIO-HE dams gaining 119% more weight than DR-chow (P < 0.001). A high-resolution analysis of maternal behaviors did not detect main effects of diet or phenotype, but a subset of DIO dams showed delayed nursing behavior (P < 0.05). In generation F6/F7 dams, effects on gestational weight gain persisted (P < 0.01), and we observed a main effect of phenotype during a sucrose preference test (P < 0.05), with DI
- Published
- 2022
10. The Deubiquitinase OTUB1 Is a Key Regulator of Energy Metabolism
- Author
-
Ruiz-Serrano, Amalia, Boyle, Christina N; https://orcid.org/0000-0002-1060-2529, Monné Rodríguez, Josep M; https://orcid.org/0000-0002-2574-0780, Günter, Julia; https://orcid.org/0000-0002-9397-5653, Jucht, Agnieszka E; https://orcid.org/0000-0002-4553-4319, Pfundstein, Svende, Bapst, Andreas M, Lutz, Thomas A; https://orcid.org/0000-0002-5056-8548, Wenger, Roland H; https://orcid.org/0000-0001-7592-4839, Scholz, Carsten C; https://orcid.org/0000-0001-6579-8015, Ruiz-Serrano, Amalia, Boyle, Christina N; https://orcid.org/0000-0002-1060-2529, Monné Rodríguez, Josep M; https://orcid.org/0000-0002-2574-0780, Günter, Julia; https://orcid.org/0000-0002-9397-5653, Jucht, Agnieszka E; https://orcid.org/0000-0002-4553-4319, Pfundstein, Svende, Bapst, Andreas M, Lutz, Thomas A; https://orcid.org/0000-0002-5056-8548, Wenger, Roland H; https://orcid.org/0000-0001-7592-4839, and Scholz, Carsten C; https://orcid.org/0000-0001-6579-8015
- Abstract
Dysregulated energy metabolism is a major contributor to a multitude of pathologies, including obesity and diabetes. Understanding the regulation of metabolic homeostasis is of utmost importance for the identification of therapeutic targets for the treatment of metabolically driven diseases. We previously identified the deubiquitinase OTUB1 as substrate for the cellular oxygen sensor factor-inhibiting HIF (FIH) with regulatory effects on cellular energy metabolism, but the physiological relevance of OTUB1 is unclear. Here, we report that the induced global deletion of OTUB1 in adult mice (Otub1 iKO) elevated energy expenditure, reduced age-dependent body weight gain, facilitated blood glucose clearance and lowered basal plasma insulin levels. The respiratory exchange ratio was maintained, indicating an unaltered nutrient oxidation. In addition, Otub1 deletion in cells enhanced AKT activity, leading to a larger cell size, higher ATP levels and reduced AMPK phosphorylation. AKT is an integral part of insulin-mediated signaling and Otub1 iKO mice presented with increased AKT phosphorylation following acute insulin administration combined with insulin hypersensitivity. We conclude that OTUB1 is an important regulator of metabolic homeostasis.
- Published
- 2022
11. Influence of High Energy Diet and Polygenic Predisposition for Obesity on Postpartum Health in Rat Dams
- Author
-
Leuthardt, Andrea S., primary, Bayer, Julia, additional, Monné Rodríguez, Josep M., additional, and Boyle, Christina N., additional
- Published
- 2022
- Full Text
- View/download PDF
12. Vaccination-based immunotherapy to target profibrotic cells in liver and lung
- Author
-
Sobecki, Michal, Chen, Jing M, Krzywinska, Ewelina, Nagarajan, Shunmugam, Fan, Zheng, Nelius, Eric, Monné Rodríguez, Josep M, Seehusen, Frauke, Hussein, Amro, Moschini, Greta, Hajam, Edries Y, Kiran, Ravi, Gotthardt, Dagmar, Debbache, Julien, Badoual, Cécile, Sato, Tatsuyuki, Isagawa, Takayuki, Takeda, Norihiko, Tanchot, Corinne, Tartour, Eric, Weber, Achim, Werner, Sabine, Loffing, Johannes, Sommer, Lukas, Sexl, Veronika, Münz, Christian, Feghali-Bostwick, Carol, Pachera, Elena, Distler, Oliver, and University of Zurich
- Subjects
10017 Institute of Anatomy ,10049 Institute of Pathology and Molecular Pathology ,10051 Rheumatology Clinic and Institute of Physical Medicine ,10184 Institute of Veterinary Pathology ,610 Medicine & health ,10263 Institute of Experimental Immunology - Published
- 2022
- Full Text
- View/download PDF
13. Feline hypertrophic cardiomyopathy : an out-of-hand cardias remodelling scenario?
- Author
-
Monné Rodríguez, Josep M, University of Zurich, and Monné Rodríguez, Josep M
- Subjects
UZHDISS UZH Dissertations ,570 Life sciences ,biology ,10184 Institute of Veterinary Pathology - Published
- 2021
14. OTUB1 regulates lung development, adult lung tissue homeostasis, and respiratory control
- Author
-
Ruiz‐Serrano, Amalia, primary, Monné Rodríguez, Josep M., additional, Günter, Julia, additional, Sherman, Samantha P. M., additional, Jucht, Agnieszka E., additional, Fluechter, Pascal, additional, Volkova, Yulia L., additional, Pfundstein, Svende, additional, Pellegrini, Giovanni, additional, Wagner, Carsten A., additional, Schneider, Christoph, additional, Wenger, Roland H., additional, and Scholz, Carsten C., additional
- Published
- 2021
- Full Text
- View/download PDF
15. Perfusate-adsorption during ex vivo lung perfusion improves early post-transplant lung function
- Author
-
Iskender, Ilker, Arni, Stephan, Maeyashiki, Tatsuo, Citak, Necati, Sauer, Mareike, Monné Rodríguez, Josep M, Frauenfelder, Thomas, Opitz, Isabelle, Weder, Walter, Inci, Ilhan, and University of Zurich
- Subjects
10042 Clinic for Diagnostic and Interventional Radiology ,10255 Clinic for Thoracic Surgery ,570 Life sciences ,biology ,10184 Institute of Veterinary Pathology - Published
- 2021
16. Constitutive depletion of Slc34a2/NaPi-IIb in rats causes perinatal mortality
- Author
-
Pastor-Arroyo, Eva Maria, Monné Rodríguez, Josep M; https://orcid.org/0000-0002-2574-0780, Pellegrini, Giovanni; https://orcid.org/0000-0001-9593-5578, Bettoni, Carla, Levi, Moshe; https://orcid.org/0000-0001-6403-2261, Hernando, Nati; https://orcid.org/0000-0002-1692-0544, Wagner, Carsten A, Pastor-Arroyo, Eva Maria, Monné Rodríguez, Josep M; https://orcid.org/0000-0002-2574-0780, Pellegrini, Giovanni; https://orcid.org/0000-0001-9593-5578, Bettoni, Carla, Levi, Moshe; https://orcid.org/0000-0001-6403-2261, Hernando, Nati; https://orcid.org/0000-0002-1692-0544, and Wagner, Carsten A
- Abstract
Absorption of dietary phosphate (Pi) across intestinal epithelia is a regulated process mediated by transcellular and paracellular pathways. Although hyperphosphatemia is a risk factor for the development of cardiovascular disease, the amount of ingested Pi in a typical Western diet is above physiological needs. While blocking intestinal absorption has been suggested as a therapeutic approach to prevent hyperphosphatemia, a complete picture regarding the identity and regulation of the mechanism(s) responsible for intestinal absorption of Pi is missing. The Na$^{+}$/Pi cotransporter NaPi-IIb is a secondary active transporter encoded by the Slc34a2 gene. This transporter has a wide tissue distribution and within the intestinal tract is located at the apical membrane of epithelial cells. Based on mouse models deficient in NaPi-IIb, this cotransporter is assumed to mediate the bulk of active intestinal absorption of Pi. However, whether or not this is also applicable to humans is unknown, since human patients with inactivating mutations in SLC34A2 have not been reported to suffer from Pi depletion. Thus, mice may not be the most appropriate experimental model for the translation of intestinal Pi handling to humans. Here, we describe the generation of a rat model with Crispr/Cas-driven constitutive depletion of Slc34a2. Slc34a2 heterozygous rats were indistinguishable from wild type animals under standard dietary conditions as well as upon 3 days feeding on low Pi. However, unlike in humans, homozygosity resulted in perinatal lethality.
- Published
- 2021
17. OTUB1 regulates lung development, adult lung tissue homeostasis, and respiratory control
- Author
-
Ruiz-Serrano, Amalia, Monné Rodríguez, Josep M, Günter, Julia, Sherman, Samantha P M, Jucht, Agnieszka E; https://orcid.org/0000-0002-4553-4319, Fluechter, Pascal, Volkova, Yulia L; https://orcid.org/0000-0001-5772-2889, Pfundstein, Svende, Pellegrini, Giovanni, Wagner, Carsten A; https://orcid.org/0000-0002-9874-8898, Schneider, Christoph, Wenger, Roland H; https://orcid.org/0000-0001-7592-4839, Scholz, Carsten C; https://orcid.org/0000-0001-6579-8015, Ruiz-Serrano, Amalia, Monné Rodríguez, Josep M, Günter, Julia, Sherman, Samantha P M, Jucht, Agnieszka E; https://orcid.org/0000-0002-4553-4319, Fluechter, Pascal, Volkova, Yulia L; https://orcid.org/0000-0001-5772-2889, Pfundstein, Svende, Pellegrini, Giovanni, Wagner, Carsten A; https://orcid.org/0000-0002-9874-8898, Schneider, Christoph, Wenger, Roland H; https://orcid.org/0000-0001-7592-4839, and Scholz, Carsten C; https://orcid.org/0000-0001-6579-8015
- Abstract
OTUB1 is one of the most highly expressed deubiquitinases, counter-regulating the two most abundant ubiquitin chain types. OTUB1 expression is linked to the development and progression of lung cancer and idiopathic pulmonary fibrosis in humans. However, the physiological function of OTUB1 is unknown. Here, we show that constitutive whole-body Otub1 deletion in mice leads to perinatal lethality by asphyxiation. Analysis of (single-cell) RNA sequencing and proteome data demonstrated that OTUB1 is expressed in all lung cell types with a particularly high expression during late-stage lung development (E16.5, E18.5). At E18.5, the lungs of animals with Otub1 deletion presented with increased cell proliferation that decreased saccular air space and prevented inhalation. Flow cytometry-based analysis of E18.5 lung tissue revealed that Otub1 deletion increased proliferation of major lung parenchymal and mesenchymal/other non-hematopoietic cell types. Adult mice with conditional whole-body Otub1 deletion (wbOtub1del/del ) also displayed increased lung cell proliferation in addition to hyperventilation and failure to adapt the respiratory pattern to hypoxia. On the molecular level, Otub1 deletion enhanced mTOR signaling in embryonic and adult lung tissues. Based on these results, we propose that OTUB1 is a negative regulator of mTOR signaling with essential functions for lung cell proliferation, lung development, adult lung tissue homeostasis, and respiratory regulation. Keywords: FIH; HIF1AN; deubiquitinating enzyme; hypoxia; respiratory distress syndrome; respiratory failure.
- Published
- 2021
18. Preclinical evaluation of 5-methyltetrahydrofolate-based radioconjugates—new perspectives for folate receptor–targeted radionuclide therapy
- Author
-
Guzik, Patrycja, primary, Benešová, Martina, additional, Ratz, Magdalena, additional, Monné Rodríguez, Josep M., additional, Deberle, Luisa M., additional, Schibli, Roger, additional, and Müller, Cristina, additional
- Published
- 2020
- Full Text
- View/download PDF
19. Therapeutic Potential of 47Sc in Comparison to 177Lu and 90Y: Preclinical Investigations
- Author
-
Siwowska, Klaudia, primary, Guzik, Patrycja, additional, Domnanich, Katharina A., additional, Monné Rodríguez, Josep M., additional, Bernhardt, Peter, additional, Ponsard, Bernard, additional, Hasler, Roger, additional, Borgna, Francesca, additional, Schibli, Roger, additional, Köster, Ulli, additional, van der Meulen, Nicholas P., additional, and Müller, Cristina, additional
- Published
- 2019
- Full Text
- View/download PDF
20. Immunohistochemical expression of MDR1-Pgp 170 in canine cutaneous and oral melanomas: pattern of expression and association with tumour location and phenotype
- Author
-
Finotello, R, Monné Rodríguez, Josep M, Vilafranca, M, Altimira, J, Ramirez, G A, Haines, A, Ressel, L, University of Zurich, and Finotello, R
- Subjects
General Veterinary ,3400 General Veterinary ,570 Life sciences ,biology ,10184 Institute of Veterinary Pathology - Published
- 2017
21. Therapeutic potential of 47Sc in comparison to 177Lu and 90Y: Preclinical investigations
- Author
-
Siwowska, Klaudia, Guzik, Patrycja, Domnanich, Katharina A., Monné Rodríguez, Josep M., Bernhardt, Peter, Ponsard, Bernard, Hasler, Roger, Borgna, Francesca, Schibli, Roger, Köster, Ulli, van der Meulen, Nicholas P., and Müller, Cristina
- Subjects
folate receptor ,SPECT ,47Sc ,radionuclide therapy ,177Lu ,IGROV-1 tumor xenografts ,90Y ,DOTA-folate ,3. Good health ,preclinical therapy - Abstract
Targeted radionuclide therapy with 177Lu- and 90Y-labeled radioconjugates is a clinically-established treatment modality for metastasized cancer. 47Sc is a therapeutic radionuclide that decays with a half-life of 3.35 days and emits medium-energy β−-particles. In this study, 47Sc was investigated, in combination with a DOTA-folate conjugate, and compared to the therapeutic properties of 177Lu-folate and 90Y-folate, respectively. In vitro, 47Sc-folate demonstrated effective reduction of folate receptor-positive ovarian tumor cell viability similar to 177Lu-folate, but 90Y-folate was more potent at equal activities due to the higher energy of emitted β−-particles. Comparable tumor growth inhibition was observed in mice that obtained the same estimated absorbed tumor dose (~21 Gy) when treated with 47Sc-folate (12.5 MBq), 177Lu-folate (10 MBq), and 90Y-folate (5 MBq), respectively. The treatment resulted in increased median survival of 39, 43, and 41 days, respectively, as compared to 26 days in untreated controls. There were no statistically significant differences among the therapeutic effects observed in treated groups. Histological assessment revealed no severe side effects two weeks after application of the radiofolates, even at double the activity used for therapy. Based on the decay properties and our results, 47Sc is likely to be comparable to 177Lu when employed for targeted radionuclide therapy. It may, therefore, have potential for clinical translation and be of particular interest in tandem with 44Sc or 43Sc as a diagnostic match, enabling the realization of radiotheragnostics in future., Pharmaceutics, 11 (8)
22. Preclinical evaluation of 5-methyltetrahydrofolate-based radioconjugates-new perspectives for folate receptor-targeted radionuclide therapy
- Author
-
Guzik, Patrycja, Benešová, Martina, Ratz, Magdalena, Monné Rodríguez, Josep M., Deberle, Luisa M., Schibli, Roger, and Müller, Cristina
- Subjects
5-Methyltetrahydrofolate ,Albumin-binding entity ,Folate receptor ,Lutetium-177 ,Radionuclide therapy ,3. Good health - Abstract
Purpose The folate receptor (FR) is frequently overexpressed in a variety of tumor types and, hence, an interesting target for radionuclide therapy. The aim of this study was to evaluate a new class of albumin-binding radioconjugates comprising 5-methyltetrahydrofolate (5-MTHF) as a targeting agent and to compare their properties with those of the previously established folic acid-based [Lu-177]Lu-OxFol-1. Methods [Lu-177]Lu-6R-RedFol-1 and [Lu-177]Lu-6S-RedFol-1 were investigated in vitro using FR-positive KB tumor cells. Biodistribution studies were performed in KB tumor-bearing mice, and the areas under the curve (AUC(0 -> 120h)) were determined for the uptake in tumors and kidneys. [Lu-177]Lu-6R-RedFol-1 was compared with [Lu-177]Lu-OxFol-1 in a therapy study over 8 weeks using KB tumor-bearing mice. Results Both radioconjugates demonstrated similar in vitro properties as [Lu-177]Lu-OxFol-1; however, the tumor uptake of [Lu-177]Lu-6R-RedFol-1 and [Lu-177]Lu-6S-RedFol-1 was significantly increased in comparison with [Lu-177]Lu-OxFol-1. In the case of [Lu-177]Lu-6S-RedFol-1, also the kidney uptake was increased; however, renal retention of [Lu-177]Lu-6R-RedFol-1 was similar to that of [Lu-177]Lu-OxFol-1. This led to an almost 4-fold increased tumor-to-kidney AUC(0 -> 120h)ratio of [Lu-177]Lu-6R-RedFol-1 as compared with [Lu-177]Lu-6S-RedFol-1 and [Lu-177]Lu-OxFol-1. At equal activity, the therapeutic effect of [Lu-177]Lu-6R-RedFol-1 was better than that of [Lu-177]Lu-OxFol-1, reflected by a slower tumor growth and, consequently, an increased median survival time (49 days vs. 34 days). Conclusion This study demonstrated the promising potential of 5-MTHF-based radioconjugates for FR-targeting. Application of [Lu-177]Lu-6R-RedFol-1 resulted in unprecedentedly high tumor-to-kidney ratios and, as a consequence, a superior therapeutic effect as compared with [Lu-177]Lu-OxFol-1. These findings, together with the absence of early side effects, make [Lu-177]Lu-6R-RedFol-1 attractive in view of a future clinical translation., European Journal of Nuclear Medicine and Molecular Imaging, 48 (4), ISSN:1619-7070, ISSN:1619-7089
23. Cardiac Truncus Arteriosus in an Eastern Black Rhinoceros (Diceros bicornis michaeli)
- Author
-
Ranieri Verin, Julian Chantrey, J.M. Monné Rodriguez, Steve Unwin, University of Zurich, and Monné Rodríguez, Josep M
- Subjects
congenital, hereditary, and neonatal diseases and abnormalities ,040301 veterinary sciences ,Heart malformation ,3400 General Veterinary ,Day of life ,Eastern black rhinoceros (Diceros bicornis michaeli) ,Histopathology ,10184 Institute of Veterinary Pathology ,Cardiac Truncus Arteriosus ,030204 cardiovascular system & hematology ,Truncus arteriosus ,Pathology and Forensic Medicine ,0403 veterinary science ,Gross examination ,03 medical and health sciences ,0302 clinical medicine ,medicine.artery ,Medicine ,Animals ,cardiovascular diseases ,Perissodactyla ,Black rhinoceros ,Aorta ,Persistent Truncus Arteriosus ,biology ,General Veterinary ,business.industry ,04 agricultural and veterinary sciences ,Anatomy ,biology.organism_classification ,Truncus Arteriosus, Persistent ,2734 Pathology and Forensic Medicine ,medicine.anatomical_structure ,Ventricle ,cardiovascular system ,570 Life sciences ,business - Abstract
Summary This case report describes congenital truncus arteriosus in a 1-month-old Eastern black rhinoceros (Diceros bicornis michaeli). From the first day of life the animal was underweight and from the 22nd day of life displayed respiratory signs that exacerbated with time leading eventually to collapse and death. Post-mortem examination revealed a single truncus arteriosus originating from the right ventricle leading to two separated pulmonary arteries and the aorta, with the ventricular septum showing a focal communicating defect. Based on the gross examination and current human classifications, the truncus arteriosus was classified as type III or A2. This is the first description of persistent truncus arteriosus in an Eastern black rhinoceros.
- Published
- 2017
24. The Deubiquitinase OTUB1 Is a Key Regulator of Energy Metabolism.
- Author
-
Ruiz-Serrano A, Boyle CN, Monné Rodríguez JM, Günter J, Jucht AE, Pfundstein S, Bapst AM, Lutz TA, Wenger RH, and Scholz CC
- Subjects
- Adenylate Kinase metabolism, Animals, Blood Glucose, Body Weight, Cell Size, Cells, Cultured, Cysteine Endopeptidases metabolism, Energy Metabolism, Fibroblasts cytology, Fibroblasts drug effects, Fibroblasts metabolism, Insulin adverse effects, Mice, Phosphorylation, Proto-Oncogene Proteins c-akt metabolism, Adenosine Triphosphate metabolism, Cysteine Endopeptidases genetics, Gene Deletion, Insulin administration & dosage, Insulin Resistance genetics, Mixed Function Oxygenases metabolism
- Abstract
Dysregulated energy metabolism is a major contributor to a multitude of pathologies, including obesity and diabetes. Understanding the regulation of metabolic homeostasis is of utmost importance for the identification of therapeutic targets for the treatment of metabolically driven diseases. We previously identified the deubiquitinase OTUB1 as substrate for the cellular oxygen sensor factor-inhibiting HIF (FIH) with regulatory effects on cellular energy metabolism, but the physiological relevance of OTUB1 is unclear. Here, we report that the induced global deletion of OTUB1 in adult mice ( Otub1 iKO) elevated energy expenditure, reduced age-dependent body weight gain, facilitated blood glucose clearance and lowered basal plasma insulin levels. The respiratory exchange ratio was maintained, indicating an unaltered nutrient oxidation. In addition, Otub1 deletion in cells enhanced AKT activity, leading to a larger cell size, higher ATP levels and reduced AMPK phosphorylation. AKT is an integral part of insulin-mediated signaling and Otub1 iKO mice presented with increased AKT phosphorylation following acute insulin administration combined with insulin hypersensitivity. We conclude that OTUB1 is an important regulator of metabolic homeostasis.
- Published
- 2022
- Full Text
- View/download PDF
25. Therapeutic Potential of 47 Sc in Comparison to 177 Lu and 90 Y: Preclinical Investigations.
- Author
-
Siwowska K, Guzik P, Domnanich KA, Monné Rodríguez JM, Bernhardt P, Ponsard B, Hasler R, Borgna F, Schibli R, Köster U, van der Meulen NP, and Müller C
- Abstract
Targeted radionuclide therapy with
177 Lu- and90 Y-labeled radioconjugates is a clinically-established treatment modality for metastasized cancer.47 Sc is a therapeutic radionuclide that decays with a half-life of 3.35 days and emits medium-energy β- -particles. In this study,47 Sc was investigated, in combination with a DOTA-folate conjugate, and compared to the therapeutic properties of177 Lu-folate and90 Y-folate, respectively. In vitro,47 Sc-folate demonstrated effective reduction of folate receptor-positive ovarian tumor cell viability similar to177 Lu-folate, but90 Y-folate was more potent at equal activities due to the higher energy of emitted β- -particles. Comparable tumor growth inhibition was observed in mice that obtained the same estimated absorbed tumor dose (~21 Gy) when treated with47 Sc-folate (12.5 MBq),177 Lu-folate (10 MBq), and90 Y-folate (5 MBq), respectively. The treatment resulted in increased median survival of 39, 43, and 41 days, respectively, as compared to 26 days in untreated controls. There were no statistically significant differences among the therapeutic effects observed in treated groups. Histological assessment revealed no severe side effects two weeks after application of the radiofolates, even at double the activity used for therapy. Based on the decay properties and our results,47 Sc is likely to be comparable to177 Lu when employed for targeted radionuclide therapy. It may, therefore, have potential for clinical translation and be of particular interest in tandem with44 Sc or43 Sc as a diagnostic match, enabling the realization of radiotheragnostics in future.- Published
- 2019
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.